Labshake search
Citations for Thermo Fisher :
4051 - 4100 of 5346 citations for Fab pHast human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Globin mRNA was depleted from a portion of each total RNA sample using the GLOBINclear™-Human kit (Thermo Fisher). Following the removal of globin transcripts transcriptome profiles were generated via mRNA sequencing ...
-
bioRxiv - Cell Biology 2022Quote: Human and mouse lung samples were inflated with a 4% w/v paraformaldehyde (PFA) solution in PBS (14190250, Thermo Fisher) then placed in 4% PFA overnight for fixation ...
-
bioRxiv - Bioengineering 2022Quote: ... The xeno-free epidermal bioink was formulated with 2 x 106/ mL human KCs in 500 μL of EpiLife medium (#MEPI500CA; ThermoFisher) supplemented with HKSdaFREE and HKGE (AvantBio ...
-
bioRxiv - Microbiology 2022Quote: Human monocytes/macrophages U937 and Thp1 were cultured in RPMI-Roswell Park Memorial Institute media supplemented with 10% FBS (Gibco) at 37°C in a humidified incubator (Panasonic ...
-
bioRxiv - Molecular Biology 2022Quote: Wild type and MED14-dTAG human colorectal carcinoma HCT-116 cells [25] were grown in RPMI 1640 medium (Gibco, 21875034) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
-
bioRxiv - Bioengineering 2022Quote: Human iPSC-derived Brain Microvascular Endothelial-like cells were treated with 25 μg/mL fluorescent transferrin conjugate (Thermo Fisher Scientific) and incubated at 37oC for 30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3 times with PBS and incubated for 1 hour with Alexa 488 nm or Alexa 594 nm conjugated secondary antibodies specific for human and rat IgG (Invitrogen) diluted 1:2,000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
-
bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney 293T (HEK 293T) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco™, Cat no. #12800017) supplemented with 10% (v/v ...
-
bioRxiv - Immunology 2022Quote: ... The abundance of select cytokines was then measured in 50μL of the supernatants with a Human Cytokine Magnetic 10-Plex Panel (ThermoFisher, LHC0001M) according to the instructions of the manufacturer ...
-
bioRxiv - Cancer Biology 2022Quote: ... the ST1 cells were transfected with NICD pCMV Neo/intracellular domain of human Notch1 (NIC-1) plasmid using Lipofectamine 200 (Invitrogen), and then were harvested 48 hours after transfection for western blot and in vitro angiogenesis assay.
-
bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
-
bioRxiv - Cancer Biology 2022Quote: ... as well as NCM-356 (ATCC CVCL-D875) non-transformed human colonic mucosal cells were maintained in McCoy’s media (Thermo Scientific) supplemented with 10% FBS (Gemini Bio-Products) ...
-
bioRxiv - Immunology 2020Quote: DNA encoding antibody variable domains was amplified by PCR from the pHEN1 phage display vector and cloned into a human IgG1 expression vector based on pCEP4 (Invitrogen). After validation of the cloning by Sanger sequencing ...
-
bioRxiv - Neuroscience 2020Quote: The human cells SH-SY5Y were grown in D-MEM (Dulbecco’s Modified Eagle medium; Gibco®, Life technologies Paisley, UK) supplemented with 10% fetal bovine serum and 1% of penicillin/streptomycin solution (100 U/mL) ...
-
bioRxiv - Neuroscience 2020Quote: The human cells SH-SY5Y were grown in D-MEM (Dulbecco’s Modified Eagle medium; Gibco®, Life technologies Paisley, UK) supplemented with 10% fetal bovine serum and 1% of penicillin/streptomycin solution (100 U/mL) ...
-
bioRxiv - Neuroscience 2020Quote: RNA from human cerebral organoids was extracted according to the manufacturer’s protocol supplied with TRIzol reagent (15596018, Thermo Fisher Scientific). The concentration and purity of the RNA samples were measured using Spectrophotometer (Beckman Coulter).
-
bioRxiv - Molecular Biology 2021Quote: ... and S309 and VHH72-Fc or variants thereof was detected with an AF633 conjugated goat anti-human IgG antibody (Invitrogen). Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec ...
-
bioRxiv - Biochemistry 2021Quote: ... the cells in each well were transfected with 20 pmol siRNA targeting to human XPO1 (GenePharma, Shanghai, China) using lipofectamine 2000 (Invitrogen). At 6 h after transfection ...
-
bioRxiv - Molecular Biology 2020Quote: Efficiency of all qPCR probes was tested by using a simplex 2-fold serial dilution of Quantigene commercial reference human RNA (Invitrogen) or DNA (Promega ...
-
bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
-
bioRxiv - Biochemistry 2020Quote: In vitro 3D epidermal skin equivalents were generated as previously described.[28] Human neonatal keratinocytes (HEKn, ThermoFisher Scientific, Loughborough, UK) were maintained as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: We purified polyclonal IgG from human plasma samples using Pierce™ Protein G Ultra Link™ Resin (Thermo Fisher Scientific) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... Fibrin formation was visualized by spiking whole blood with human fibrinogen conjugated to Alexa Fluor 647 (Cat. No. F35200, Invitrogen, used at final concentration of 7.5 μg/mL) ...
-
bioRxiv - Cancer Biology 2021Quote: ... PLK2 in a pDONR223 vector was obtained from human ORFeome (Open Biosystems-Horizon Discovery) and was cloned into pCW57.1 using the Gateway recombination reaction (Life Technologies) and verified by sequencing prior to transfection ...
-
bioRxiv - Neuroscience 2020Quote: ... and the SH-SY5Y human neuroblastoma cell line was maintained in 10% FBS Minimum Essential/F12 medium (MEM/F12, Gibco). Both cells’ media included 1% antibiotic/antimycotic mix (Gibco) ...
-
bioRxiv - Microbiology 2021Quote: ADAP1CRISPR and CtrlCRISPR TM were left resting or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
-
bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously21 ...
-
bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
-
bioRxiv - Immunology 2020Quote: ... Acquired DDA .raw files were searched against the human UniProtKB/SwissProt database (v2012_07) using Byonic (Protein Metrics) embedded in Proteome Discoverer (ThermoFisher Scientific) to obtain peptide sequence information ...
-
bioRxiv - Biochemistry 2021Quote: ... The slide was washed thrice with PBS-T for 1 minute and further incubated with goat anti-human IgG (Fc)-Cy5 (Invitrogen) antibodies at 1:2500 dilution ...
-
bioRxiv - Microbiology 2021Quote: Individual plasmids encoding 11 human genes or empty control vector were transfected individually into U-87 MG cells with Lipofectamine 3000 (Invitrogen) 48 hours prior to infection with VEEV-TC-83-nLuc.
-
bioRxiv - Microbiology 2021Quote: Huh-7 cells seeded in 6 well plates were transfected with human WWOX-specific or random siRNAs (DHARMACON) at a final concentration of 50nM per well using Lipofectamine 2000 reagent (INVITROGEN). At 24 hours post siRNA transfection ...
-
bioRxiv - Microbiology 2022Quote: ... Primary antibodies were detected by probing with biotin-conjugated anti-rat antibody (1:1,000) and Alexa 488-conjugated anti-human antibody (1:1,000) followed by Alexa 594-conjugated streptavidin (Invitrogen, 1:1000). Slides were then stained with 1ug/mL DAPI ...
-
bioRxiv - Microbiology 2022Quote: ... gondii parasites of the type I RH strain Δku80/Δhxgprt genetic background (ATCC PRA-319) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) maintained in DMEM (GIBCO) supplemented with 3% inactivated fetal calf serum (IFS ...
-
bioRxiv - Genetics 2022Quote: ... Genotyping was done using Axiom™ Human Genotyping SARS-CoV-2 Research Array as instructed by the manufacturer (Thermo Scientific).
-
bioRxiv - Immunology 2022Quote: A recombinant fragment of human SP-D (rfhSP-D) was expressed under bacteriophage T7 promoter in Escherichia coli BL21 (λDE3) pLysS (Invitrogen), transformed with for plasmid containing cDNA sequences for neck ...
-
bioRxiv - Immunology 2022Quote: Human bronchial epithelial cells (BEAS-2B cells, ATCC® CRL-9609™) were cultured in LHC-9 medium (Life Technologies) and human lung fibroblast (MRC-5 cells ...
-
bioRxiv - Immunology 2022Quote: ... were generously provided by Anita Koshy (University of Arizona) and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco] ...
-
bioRxiv - Immunology 2022Quote: ... and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco], 20% Medium 199 [Gibco], 10% FBS [Gibco] ...
-
bioRxiv - Immunology 2022Quote: ... and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco], 20% Medium 199 [Gibco] ...
-
bioRxiv - Genetics 2022Quote: ... hiPSC-CMs were then transfected with siRNAs directed against PAX9 and EGR2 (ON-TARGETplus Human) using lipofectamine RNAi Max (ThermoFisher). Each siRNA was tested individually in 8-plicates ...
-
bioRxiv - Cell Biology 2022Quote: Toxoplasma gondii RHΔhxgprt strain was maintained by growth in confluent primary human foreskin fibroblasts (HFFs) in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2022Quote: ... Sorted Tregs were pre-activated for 48h using α-CD3/CD28 coated beads (Dynabeads Human T-Activator, Thermo Fisher scientific) in a 1:1 ratio (beads to cells) ...
-
bioRxiv - Microbiology 2022Quote: Murine macrophage-like RAW 264.7 cells and human A549 lung epithelial carcinoma cells were cultivated in RPMI 1640 medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2019Quote: BJ-5ta hTERT-immortalized human fibroblasts were licensed from ATCC (CRL-4001) and cultivated in Dulbecco’s Modified Eagles Medium Glutamax® (Thermofisher) with 20% Medium 199 (Earles Salts ...
-
bioRxiv - Immunology 2019Quote: ... T cells were stimulated for 2 days at a starting density of approximately 1 million cells per mL of media with anti-human CD3/CD28 Dynabeads (ThermoFisher), at a bead to cell ratio of 1:1 ...
-
bioRxiv - Microbiology 2019Quote: ... and HEK293T (embryonic human kidney epithelial, ATCC®CRL-3216) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Microbiology 2019Quote: ... The immortalized human upper airway epithelial cell line A549 (ATCC# CCL-185) was maintained in minimal essential medium (MEM; Gibco) supplemented with 10% fetal bovine serum ...
-
Peptide Arrays of Three Collections of Human Sera from Patients Infected with Mosquito-Borne VirusesbioRxiv - Microbiology 2019Quote: ... The arrays were then incubated for 1 h with goat anti-human IgG tagged with Alexa Fluor® 488 (Invitrogen) as secondary antibody ...