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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for CD8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Viral N protein was detected using a rabbit anti-SARS-CoV N antibody [32] as a primary antibody with AlexaFluor 568 anti-rabbit IgG or anti-rabbit IgG-HRP (Thermo Fisher) as secondary antibodies together with DAPI to stain the nucleus by indirect immunofluorescence as described previously [33].
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bioRxiv - Immunology 2020Quote: ... PBMCs were fixed at 48hpi and stained intracellularly using CHIKV E1-specific rabbit antibody (kindly gifted by dr. G. Pijlman from Wageningen University) and secondary chicken anti-rabbit AF647 (Life Technologies). Samples were measured on Aurora flow cytometer (Cytek®) ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-IgG 1:500, rabbit anti-Dp 212 1:500 (Dimova et al, 2003) and Dynabeads protein G (Invitrogen # 10003D). Chromatin precipitate was eluted from Dynabeads with elution buffer 1 (10mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... we confirmed targeting of the optic-fiber-tetrode bundle to LC by colocalization of the electrolytic lesion with immunostaining against tyrosine hydroxylase (rabbit anti-TH, ab152, Millipore, followed by donkey anti-rabbit Alexa 488, Life Technologies, or goat anti-rabbit Alexa 594 ...
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bioRxiv - Neuroscience 2022Quote: ... matched sections were labelled in parallel with rabbit anti-μOR (1:1000, immunoStar) followed by donkey anti-rabbit Alexa Fluor 647 (1:500, Thermo Fisher). Image data were acquired using a 20X objective and z-stack tile approach on a Zeiss LSM700 confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: ... A subset of sections was stained for calbindin+ cells (1⁰ rabbit anti-calbindin (Swant #CB38) 1:500 + 2⁰ anti-rabbit-633 (Invitrogen #A21071) 1:500) ...
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bioRxiv - Neuroscience 2024Quote: ... 1:750, 226004, Synaptic Systems; rabbit anti-PV, 1:1000, ab11427, ITK; or rabbit anti-HDAC2, 3 µg/µL, AB_2533908, Thermo Fisher) in PBS-BT for 18 hours at RT ...
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bioRxiv - Microbiology 2024Quote: ... respectively as described above followed by incubation with a goat anti-rabbit fluorescent secondary antibody (Invitrogen Goat anti-Rabbit Alexa Fluor Plus 568™, Cat# A-11036, 1:1000 or Invitrogen Goat anti-Rabbit Alexa Fluor™ Plus 647 ...
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bioRxiv - Immunology 2023Quote: ... or with rabbit-anti-human TG3 (PA596450, Thermo Fischer Scientific) followed by detection with Alexa 488 donkey anti-rabbit (Molecular Probes) antibody ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The secondary antibodies were diluted to 1:250 (Goat anti-rabbit IgG Cy5; Invitrogen A10523; goat anti-mouse IgG AlexaFluor 488, Invitrogen A11029; goat anti-rabbit IgG AlexaFluor 488, Invitrogen A11034). Nuclei were stained with Hoechst 33258 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... were selected and processed as described above with primary antibody Rabbit anti-Cox-2 (1:1k, Cayman Chem, 160123) and secondary antibody AF555 donkey anti-rabbit (1:200, ThermoFisher, A31572).
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated for 2 h with Alexa Fluor 488 donkey anti-rabbit IgG or Alexa Fluor 568 donkey anti-rabbit (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock; Goat anti rabbit AF488, Thermo Fisher Scientific A11034, RRID:AB_2576217). Slides were imaged on an ANDOR Dragonfly microscope (Oxford Instruments ...
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bioRxiv - Microbiology 2019Quote: ... followed by immunostaining with a rabbit polyclonal MUC2 antibody and goat anti-rabbit Alexa-Fluor 488 antibody (Life Technologies, Paisley, United Kingdom). Slides were imaged using a BX51 upright microscope and a Coolsnap EZ camera (OLYMPUS ...
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bioRxiv - Microbiology 2019Quote: ... The SFTSV foci were detected using rabbit anti-SFTS NP antibody (Abclon) and goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (Thermo Fisher Scientific). Viral plaques were visualized by incubation with NBT/BCIP solution (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Golgin-97 was stained with rabbit anti-GM97 (CST, #D8P2K) and visualized with goat anti-rabbit conjugated to Alexa Fluor 405 (Life Technologies, #P10994). HA-Rab29 was stained with mouse anti-HA (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated anti-rabbit (Cytiva, NA934V) or fluorescence-based (Cy3-conjugated anti-mouse or Cy5-conjugated anti-rabbit; Invitrogen, Thermo Fisher Scientific) secondaries were used at 1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated anti-rabbit (Cytiva, NA934V) or fluorescence-based (Cy3-conjugated anti-mouse or Cy5-conjugated anti-rabbit; Invitrogen, Thermo Fisher Scientific) secondaries were used at 1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Klebsiella was labelled with rabbit anti-Klebsiella serum (1:1000) and visualised with goat anti rabbit Alexa Fluor 350 life-tech (1:1000) (Molecular Probes, A11046). Fluorescence images were captured using the ×100 objective lens on a Leica DM5500 microscope equipped with the appropriate filter sets ...
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bioRxiv - Bioengineering 2021Quote: ... The antibody probes used were primary rabbit-anti-tGFP antibody (Pierce PA5−22688,) and secondary polyclonal antibody AlexaFluor-647-labeled donkey-anti-rabbit (Invitrogen A-31573). Bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... immunostained with rabbit anti-O78 antibody followed by cross absorbed goat anti-rabbit AlexaFluor 594-conjugated IgG (H+L) (ThermoFisher A-11072). Membranes with fluorescently labeled samples were excised and mounted onto glass slides with ProLong Gold (ThermoFisher Scientific P36930) ...
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bioRxiv - Cell Biology 2021Quote: ... heart sections were incubated with rabbit anti-TFEB (SC48784, 1:500 dilution) antibody for 1 h and subsequently Alexa Fluor 568 goat anti-rabbit (Invitrogen, A-21069) or Alexa Fluor 488 goat anti-mouse (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Antibodies used for immunofluorescence are as listed: rabbit polyclonal anti-p62 (MBL, PM045, lot 022) and AlexaFluor 568-conjugated anti-rabbit IgG (Invitrogen, A-11036).
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bioRxiv - Immunology 2020Quote: ... GFP-expressing ST4/74 ΔaroA was visualised by immunostaining with rabbit polyclonal anti-GFP and Alexa594 labelled anti-rabbit IgG (both Thermo Fisher Scientific). GFP-expressing ST4/74 Δ then counted in 12 sections of Peyer’s patch per mouse (n=3–4 mice/group ...
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bioRxiv - Cancer Biology 2021Quote: ... Spain)] and secondary antibodies [Alexa Fluor 568 goat anti-rabbit IgG or Alexa Fluor 647 goat anti-rabbit IgG (Life Technologies, CA, USA)] were incubated for 30 min at RT ...
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bioRxiv - Zoology 2022Quote: ... while GFP was detected using an anti-GFP rabbit polyclonal primary antibody and an Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific). Cell nuclei were counterstained with DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... Caveolin-1 rabbit polyclonal antibody (# PA1-064) and anti-pFAK (Y397) rabbit monoclonal antibody (# 44624) obtained from Thermo Fisher (Grand Island, NY).
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bioRxiv - Immunology 2020Quote: ... secondary mAbs (anti-mouse and anti-rabbit IgG H&L, Alexa Fluor® 488 - Abcam; anti-rabbit IgG H&L, Alexa Fluor® 647 - Invitrogen) was added in 0.5% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 488 goat anti-rabbit IgG and 568 anti-rabbit IgG (3% dilution in intracellular solution, A11008 and A11011 respectively, Life technologies, Germany). To visualize the Ca2+-hotspots and the presynaptic ribbons ...
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bioRxiv - Molecular Biology 2019Quote: ... and rabbit anti-D5R antibody and donkey anti-rabbit secondary antibody tagged with Alexa Fluor™ 488 (#R37118, ThermoFisher Scientific Waltham, MA). The tissues were counterstained with wheat germ agglutinin (WGA ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies were used at 1:2000 dilution and peroxidase-conjugated secondary antibodies (goat anti-rabbit, ThermoFisher Scientific #31460; rabbit anti-goat, Thermo Scientific #31402) at 1:5000 ...
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bioRxiv - Immunology 2022Quote: ... Samples were denatured and loaded onto the Jess plate with appropriate reagents and primary antibodies: Anti-S100a8 Polyclonal Antibody – Rabbit (Fisher PIPA579948) diluted 1:20 and anti-S100a9 Polyclonal Antibody – Rabbit (Invitrogen PA5-79950) diluted 1:50 ...
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bioRxiv - Microbiology 2022Quote: ... cells were permeabilized as described above and sequentially stained with primary rabbit α-GFP antibodies (1:250; MBL) and secondary goat α-rabbit Alexa Fluor 488 antibodies (2 µg/ml; Invitrogen) for 1h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... the parasite mitochondrion was visualized using a polyclonal Rabbit anti-HSP60 antibody (Novus NBP2-12734) and AlexaFluor 647-conjugated Goat anti-Rabbit 2° antibody (Invitrogen Life Technologies A21244), the nucleus was stained with ProLong Gold Antifade Mountant with DAPI (Invitrogen Life Technologies P36931) ...
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bioRxiv - Microbiology 2023Quote: ... cells were probed with rabbit anti-p239 polyclonal antibodies and Alexa Fluor®555-labeled goat anti-rabbit IgG (Thermo Fisher Scientific). Cellular nuclei were counterstained with 4□ ...
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bioRxiv - Neuroscience 2023Quote: ... (3) positive staining for IBA1 (rabbit primary, Wako, 019-1974, 1:2000, donkey anti-rabbit 647 Life Technologies, A-31573, 1:1000) via 2D immunofluorescence imaging and (4 ...
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bioRxiv - Microbiology 2024Quote: ... probed with anti-GFPmut2 primary antibody (1:1000 dilution, rabbit pAb, custom Pocono RF&L) with anti-rabbit conjugated to HRP (1:1000 dilution, Invitrogen, Cat #A16104) or streptavidin-HRP (1:1000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG, A11122 Invitrogen, 1:000, or polyclonal anti-GFP rabbit IgG, A-6455 ThermoFisher, 1:2000) antibodies were then used ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in rabbit polyclonal anti-Blbp (Suppl. Table 1) overnight followed by 1 hr in donkey anti-rabbit 555 (1:800; Life Technologies, #A31572) and coverslipped with Fluorogel ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor goat anti-rabbit 594, A11037; Alexa Fluor goat anti-rabbit 488, A11008; Alexa Fluor goat anti-rat 594, A11007; Invitrogen, Waltham, MA) before mounting with DAPI Fluoromount-G (0100-20 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-akt (9272, CellSignaling, 1:1000), rabbit anti-Phospho-akt (SER473) (9277S, CellSignaling, 1:1000) and rabbit anti-cathepsin G (703590, Invitrogen, 1:1000), overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and then stained with a rabbit polyclonal anti-TgMIC5 antibody (1:1000) and goat anti-rabbit IgG conjugated with Alexa 488 (green) (Invitrogen, 1:1000) to label all parasites ...
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bioRxiv - Cell Biology 2023Quote: ... #A-11058, 1:400; Alexa Fluor 488 anti-rabbit, Thermo Fisher, #A-21206, 1:400; Alexa Fluor 546 anti-rabbit, Thermo Fisher, #A-11056 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies used for immunofluorescence are as listed: rabbit polyclonal anti-LC3 (MBL, PM036, lot 025) and AlexaFluor 568-conjugated anti-rabbit IgG (Invitrogen, A-11036). All lipids were purchased from Avanti ...
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bioRxiv - Microbiology 2024Quote: ... was prepared by washing twice in RIPA before incubating with 15uL of anti-RIG-I rabbit serum (969) or normal rabbit serum (Life Technologies #31883) diluted in 500uL RIPA Buffer with head-over-tail mixing for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Immunology 2024Quote: All CoMiX were generated from HEK293T cells transfected with four μg of recombinant plasmid DNA using Lipofectamine-3000 (Invitrogen, Thermo Fisher Scientific BVBA, Merelbeke, Belgium) following the manufacturer’s instructions ...