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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies, CA, USA). The expression of the genes of hexokinase 2 (HK2 ...
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bioRxiv - Immunology 2020Quote: 0.5 - 1 μg of total RNA was prepared by column purification (MachereyNagel) and transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (RevertAid Reverse Transcriptase, Thermo Fisher Scientific, #EP0441) in a total volume of 10 or 20 μl ...
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bioRxiv - Physiology 2020Quote: ... One microgram of RNA was reverse transcribed into cDNA using oligo-dT and MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase, Invitrogen, Thermo Fisher Scientific). Before cDNA synthesis ...
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bioRxiv - Microbiology 2020Quote: ... diluted to 125 ml with virus dilution medium and added to confluent monolayers of Vero-E6 cells in twenty-five T175 flasks (Nunc; 5 ml/flask). After 1 hour of incubation at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Microbiology 2024Quote: ... A 10 µL RT–qPCR reaction to identify the RNA enteric viruses of interest contained 2.5 µL of fast virus 1-step master mix (4X) (Life Technologies, Carlsbad, CA, USA), 500 nM of each specific primers ...
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bioRxiv - Microbiology 2024Quote: ... half of the virus supernatant after the concentration step was digested with amplification grade DNAse I (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA), for 10 minutes at 37 °C before DNA extraction to reduce host DNA contamination ...
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bioRxiv - Microbiology 2023Quote: ... Virus detection was performed by quantitative RT-qPCR on a QuantStudio® 5 Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA), according toconditions described in Guedes et al ...
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bioRxiv - Cancer Biology 2022Quote: ... 266-6 CAS9 cells were infected with FUW-TetO-KRASG12D-BleoR virus and cells were selected with 5 mg/ml zeocin (Invitrogen, San Diego, CA) for 10 days ...
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bioRxiv - Pathology 2023Quote: ... RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Life Technologies, Carlsbad, CA, USA) on the LC96 instrument (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of c DNA from 1000 ng of pooled total RNA was carried out by using 10 μM of reverse primer from each reconstructed RNA virus and RevertAid Reverse Transcriptase (Thermo Fisher scientific, USA) (Cat ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed to detect the presence of influenza A virus (M gene) using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific). Positive influenza samples were then selected for whole genome sequencing using next generation sequencing (NGS ...
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bioRxiv - Neuroscience 2023Quote: ... The infusion line was primed with a loading line solution that was identical to the virus suspension solution [Corning phosphate-buffered saline without Ca2+ or Mg2+ with 350 mM NaCl (Gibco, ThermoFisher Scientific, Waltham, MA) containing 0.001% F68 surfactant (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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bioRxiv - Microbiology 2023Quote: ... in a total volume of 25 µL containing 5 µL of template employing Taqman Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, USA). Oligonucleotide primer and probe sequences and thermocycling conditions are detailed in Table S2 ...
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bioRxiv - Bioengineering 2022Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Synthetic Biology 2024Quote: MetBo2 (polyoma middle T oncogene–induced mouse mammary tumor on a syngeneic Friend Virus B NIH Jackson (FVB) background)[23] cells were maintained in 1X Dulbecco’s Modified Eagle Medium (DMEM) (Thermofisher Scientific; Cat. No. 11995065) with 10 % Fetal Bovine Serum (FBS ...
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bioRxiv - Systems Biology 2023Quote: ... and yield was determined by quantitative real-time PCR using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA) and TaqMan Gene Expression Assay ...
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bioRxiv - Immunology 2024Quote: ... lentiviral particles were generated through the transient transfection of plasmid into 293T virus- producing cells (ATCC, Manassas, VA, USA) in the presence of Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA), vesicular stomatitis virus G (VSV-G) ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was used to detect and determine virus titers using the AgPath ID one-step RT-PCR kit (ThermoFisher Scientific, Waltham, MA) from the cell culture supernatants as previously described [28] ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific, cat# 28025013). For quantitative Real Time Polymerase Chain Reaction (RT-qPCR) ...
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bioRxiv - Immunology 2024Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies, CA, USA). The expression of the genes HIF-1α and LDH-A was determined using the PCR SYBR Green sequence detection system (Eurogentec ...
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bioRxiv - Cancer Biology 2024Quote: One microgram of total RNA was reverse transcribed at 37□°C for 60□min in the presence of random hexamers and Moloney murine leukaemia virus reverse transcriptase (Invitrogen, Carlsbad, CA, USA). Specific oligonucleotide primers for
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bioRxiv - Cell Biology 2019Quote: ... protein was extracted using 0.2M Sodium Hydroxide and protein concentrations were determined using the Pierce™ 660 protein assay (Fisher Scientific), according to manufactures instructions ...
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bioRxiv - Pathology 2020Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coommassie Protein Assay reagent (Thermo Scientific). Pooled fractions were concentrated to 4 mL using 10 kD molecular weight cut-off spin columns (Millipore ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: Collected protein flow-throughs (FT) were subjected to protein precipitation for downstream protein content determination using bicinchoninic acid assay (BCA, Thermo Fisher) assay and trypsin protein digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentrations of the lysates were measured by BCA protein assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then unified to 0.5 µg/µL ...
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bioRxiv - Biochemistry 2022Quote: Protein concentration after cell lysis and after protein precipitation was determined using the Micro BCA™ Protein assay kit (Thermo Scientific) according to manufacturer’s guidelines.
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bioRxiv - Neuroscience 2019Quote: ... Newly synthesized proteins were then enriched using the Click-it protein enrichment kit for chemistry capture of azide modified proteins (Invitrogen C10416) following the manufactures protocol with small modifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein expression was performed according to Culturex protocol while protein purification was done using Pierce Protein A Agarose kit (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Measurement of final protein concentration was performed using BCA protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™, #23227), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Soluble marker protein levels were normalized to total protein levels measured by Quant-iT protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Immunology 2024Quote: ... Protein concentrations across all samples were normalised to the same protein concentration (500 µg/ml) with protein determination quantified using the well-established Pierce BCA Protein Assay (Thermo Fisher) following the recommended guidelines provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; ThermoFisher Scientific; cat # 23227). Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were cleared by centrifugation and protein concentration was quantified using the Qubit™ Protein and Protein Broad Range Assay Kit (ThermoFisher). Protein samples were sent to the UNSW Bioanalytical Mass Spectrometry Facility for liquid chromatography-mass spectrometry (LC-MS/MS ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were tested for protein concentration using 5 μL of protein sample mixed with 10 μL Coomassie Protein Assay reagent (ThermoFisher Scientific). Pooled fractions were concentrated to 4 mL using 10 kDa molecular weight cut-off spin columns (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration in supernatant was determined using bicinchoninic acid (BCA) protein assay (PierceTM BCA Protein Assay Kit, Catalog #23225, ThermoFisher Scientific). 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins/complex were subject to SDS-PAGE electrophoresis followed by protein staining (SYPRO Ruby Protein Gel Stain, Thermo Fisher Scientific) and immunoblotting.
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bioRxiv - Physiology 2024Quote: ... The protein content of the EV preparations was quantified by bicinchoninic acid protein assay (Pierce™ BCA Protein Assay Kit, Thermofisher).
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bioRxiv - Developmental Biology 2024Quote: ... The purified proteins were stored at −80°C and the protein concentration was determined using Bradford plus protein assay reagents (23238, Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration in the lysates was measured using a bicinchoninic acid protein assay kit (Micro BCA Protein Assay Kit, Thermo Scientific). Equal amounts (750 micrograms to 1 mg depending on the experiment ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were determined with the Pierce BCA Protein Assay Kit (Thermo Scientific), and extracts were loaded onto NuPAGE Bis–Tris SDS gels and immunoblots were visualized by LiCOR Odyssey system.
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), and the pHrodo-TDM1 fluorescent signal was normalized to the amount of protein at each time point.
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bioRxiv - Cancer Biology 2021Quote: ... The total protein concentrations were determined using BCA protein assay kit (Thermo Scientific), and IFN-α and IFN-γ protein concentrations were measured using a human IFN-α ELISA kit (1110012 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher Scientific) and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... protein concentration of the supernatant was determined using BCA protein assay (Thermo Fisher) and 50 μg (1C and S2F ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein content was quantified using a BCA Protein Assay Kit (Thermo Scientific) and equal amounts of total protein were loaded onto 4 – 15% Tris-Glycine gels (BioRad) ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were determined using the BCA protein assay (Thermo Fisher Scientific). Protein lysates were analyzed by SDS PAGE and Western blot using fluorescently-labeled secondary antibodies ...