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Citations for Thermo Fisher :
3951 - 4000 of 5129 citations for VAV2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Samples were then separated into 8 fractions using the High pH reversed-phase peptide Fractionation Kit (ThermoFisher Scientific, cat# 84868). The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Non-deuterated samples peptide identification was performed via data dependent tandem MS/MS experiments and analyzed by Proteome Discoverer 2.5 (Thermo Fisher). Mass analysis of the peptide centroids was carried by HDExaminer v3.3 (Sierra Analytics ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped in a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap 100, Thermo Fisher Scientific) with buffer A (2/98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Genetics 2023Quote: ... Peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific, Waltham, MA) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: TMT-labeled peptides were analyzed using an EASY-nLC 1200 UPLC coupled with an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). LC buffer A (0.1% formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... MS data of the peptides were acquired on a Q Exactive mass spectrometer coupled to EASY-nLC (Thermo Fisher Scientific) using a data-dependent top 10 methods that dynamically choose the most abundant precursor ions from the survey scan (300–1800 m/z ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were desalted with an Oasis PRiME HKB μElution Plate and analysed on a QExactive HF HPLC-MS (Thermo Scientific). Separation by HPLC prior to MS occurred on an Easy-nLC1200 system (Thermo Scientific ...
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bioRxiv - Physiology 2023Quote: ... the labeled sample was separated into five fractions using the high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Bound peptides were eluted with 1 ...
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bioRxiv - Biochemistry 2023Quote: We acidified the digested samples with concentrated trifluoroacetic acid (TFA) and cleaned up the peptides with C18 SOLA Solid-Phase Extraction (SPE) columns (ThermoFisher). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were further separated onto an Easy-Spray RSLC C18 column (75 µm i.d., 50 cm length, ThermoFisher Scientific) using a 30 min linear gradient (15% to 35% solvent B (0.1% formic acid in acetonitrile) ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were loaded onto a trapping cartridge (Acclaim PepMap C18 100Å, 5 mm x 300 µm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN ...
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bioRxiv - Microbiology 2023Quote: ... and peptide concentrations for each sample were estimated based on absorbance measurements at 280 nm (NanoDrop™, Thermo Fisher Scientific). For each sample ...
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bioRxiv - Immunology 2022Quote: Peptide separation in buffer A (0.1% formic acid) was achieved using an EASY-nLC 1200 HPLC system (Thermo Fisher Scientific) and a column (75 µm inner diameter ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μg of peptides from each sample were reacted for 1 h with the appropriate TMT-NHS isobaric reagent (ThermoFisher) in 40% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with warmed PBS before the addition of peptide in DMEM (without phenol red) with 10% FBS (ThermoFisher) for 2-4 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting peptides were analyzed by a top-15 data dependent LC-MS/MS strategy using a QExactive plus mass spectrometer (ThermoFisher). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were injected on a PepMap 100 C18 trap column (100 µm × 2 cm, 5 µM particles, Thermo Fisher Scientific) and separated with an EASY-Spray analytical column (PepMap RSLC C18 ...
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bioRxiv - Developmental Biology 2023Quote: ... Final peptide concentration was measured by absorbance at 280 nm on a Nanodrop ND-2000 spectrometer (Thermo Fisher, Waltham, MA).
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bioRxiv - Biochemistry 2023Quote: ... Each sample of peptides was loaded onto a C18 trap column (75 μm ID × 2 cm, 3 μm, Thermo Scientific) and then separated on a C18 analytical column (75 μm ID×50 cm ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested tryptic peptides were analyzed by LC-MS/MS on Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific) using the same configuration and settings as previously reported85 ...
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bioRxiv - Biochemistry 2023Quote: ... and applied to TiO2 spin tips (3 mg peptides per tip) for phosphopeptide enrichment according to the manufacturer’s protocol (High-SelectTM TiO2 Phosphopeptide Enrichment Kit, A32993, Thermo Scientific). Eluted phosphopeptides were transferred to Lo-bind Eppendorf tubes ...
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bioRxiv - Biochemistry 2023Quote: ... endoprotease Lys-C and Trypsin digested peptides were analyzed on an LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Bremen). The raw data were analyzed with MaxQuant (version 2.0.3.0) ...
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bioRxiv - Microbiology 2023Quote: The enriched and unenriched samples were fractionated with the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer instructions for TMT-labeled peptides ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 25 µg of peptides for each sample was labeled with 0.2 mg of TMT-10plex reagents (Thermo Fisher Scientific) for 1 h at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50 cm × 75 μm ID; Thermo Fisher Scientific). Using a constant flow rate of 300 nL/min ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA encoding the mature protein (without the signal peptide) was amplified using gene-specific primers incorporating restriction enzyme sites and subcloned into the pPICZα A vector (Invitrogen) using EcoRI and XbaI enzymes and T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... 1 µg peptides were injected on an Ultimate 3500 RSLC coupled to a Q-Exactive HF-X (Thermo Fisher Scientific). Peptides were first trapped using a PepMap 100 C18 trap column and then separated with a PepMap C18 analytical column (75 µm x 50 cm ...
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bioRxiv - Physiology 2023Quote: ... with peptides introduced via an EasySpray Nano source coupled to a Q-Exactive Plus Quadrupole Orbitrap mass spectrometer (Thermo Scientific) and subjected to data dependent tandem mass spectrometry (MS/MS) ...
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bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...
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bioRxiv - Neuroscience 2023Quote: The peptides were analysed using liquid chromatography/mass spectrometry (MS) using with an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) coupled to an UltiMate3000 RSLCnano LC system (Dionex Co. ...
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic peptides were analyzed with a Vanquish Neo UHPLC system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Peptides were separated using an increasing acetonitrile gradient from 2% to 25% over 80 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Resulting tryptic peptides were separated by reverse-phase high-performance liquid chromatography (RP-HPLC; UltimateTM 3000 RSLCnano system; Thermo Scientific) using a 50 cm analytical column (in-house packed with Poroshell 120 EC-C18 ...
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bioRxiv - Immunology 2023Quote: ... One microgram of peptide sample was loaded on a C18 column (15 cm, 3.0 μm Acclaim PepMap, Thermo Fisher Scientific), separated with a 0–40% gradient of buffer B (80% acetonitrile ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a PepMap C18 trap column (300 µm x 5 mm, 5 µm particle size, Thermo Fisher) and separated on a 50 cm Easy-Spray column (ES803 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was transferred to a new tube and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84688) following manufacturer’s recommendation.
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bioRxiv - Cancer Biology 2023Quote: ... The eluting peptides were ionized at a potential of + 2 kVa into a Q Exactive mass spectrometer (Thermo Fisher, Germany). Intact masses were measured at resolution 70.000 (at m/z 200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sep-Pak Vac tC18 solid phase extraction cartridges (Waters) and the concentration determined using the Pierce quantitative colorimetric peptide assay (ThermoFisher). Peptides were dried by vacuum centrifugation.
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bioRxiv - Biochemistry 2023Quote: ... We analyzed MS/MS data collected from ND samples to identify the likely sequence of the patent peptides using SEQUEST (Bioworks v3.3.1, Thermo Fisher Scientific) with a peptide tolerance of 8 ppm and a fragment tolerance of 0.1 AMU ...
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bioRxiv - Cell Biology 2024Quote: ... The digested peptides were then desalted using Pierce C18 Spin Tips & Columns system according to the manufacturer’s instructions (ThermoFisher Scientific). Three independent biological replicates for each condition were analyzed by mass spectrometry.
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bioRxiv - Neuroscience 2024Quote: ... Peptides were first trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Biochemistry 2024Quote: Enriched crosslinked peptides were injected onto a C18 PepMap100-trapping column (0.3 x 5 mm, 5 μm, Thermo Scientific™) connected to an in-house packed C18 analytical column (75 μm x 300 mm ...
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bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed on a EASYspray C18 column (50 cm x 75 um, Thermo Fisher Scientific, San Jose, CA) over a 65-minute gradient at a flow rate of 250 nL/min ...
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bioRxiv - Cancer Biology 2024Quote: T cells from KOG mouse TDLNs (5 × 105) were co-cultured with SIINFEKL peptide (1 μg/mL, Thermo Fisher Scientific) in a 96-well plate (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... peptides were separated on a 75 µm ID x 25 cm C18 Acclaim PepMap® RSLC column (Thermo Scientific, USA) with a 4-40% linear gradient of solvent B (0.1% formic acid in 80% ACN ...
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bioRxiv - Biochemistry 2024Quote: The tryptic peptides were injected onto a pre-column (PepMap C18, 5 mm × 300 μm × 5 μm, Thermo Fisher Scientific) and separated ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptides were desalted using Sep-Pak C18 cartridges (Waters, MA, USA) and dried in a SpeedVac (Thermo Scientific).
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched in 0.5% trifluoroacetic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/μL ...
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bioRxiv - Biochemistry 2024Quote: ... and the resulting peptides were analysed by LC-MSMS on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) using CID+ETD activation ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were digested into peptides by adding 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) solubilized in 40 µl of ABC buffer to the filters and incubating for 16 hours in a wet chamber at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µg of peptides for each sample were labeled with 50 µg of TMTpro16-plex reagents (Thermo Fisher Scientific) per channel ...