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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for Mouse Anti Hepatitis C Virus Core Protein Antibody 1862 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... rinsed 3 times in PBT and incubated for 4 h at room temperature with anti-mouse or anti-rat secondary antibody coupled to Dylight 488 (Thermo Fisher Scientific), or an secondary alpaca nanobody anti-mouse 488 SMS1AF488-1-100 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Cross-absorbed secondary antibodies Alexa Fluor™ 568nm- and 647nm-conjugated goat anti-rabbit and anti-mouse (Life Technologies #A11004 and #A21240) were used at 1/600 ...
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bioRxiv - Neuroscience 2024Quote: ... the same procedure was followed but using anti-β-Tubulin (1:1000, DSHB, USA) as the primary antibody and Goat anti-Mouse AF 546 (1:300, Invitrogen, Germany) as secondary antibody.
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bioRxiv - Molecular Biology 2024Quote: ... donkey anti-mouse Alexa Fluor 546 (1:500) or goat anti-rabbit Alexa Fluor 488 (1:500) secondary antibodies (Thermo Fisher Scientific). For staining with anti-centromere antibody ...
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bioRxiv - Microbiology 2024Quote: ... Sections were rinsed before incubation with a 1:1,000 dilution of an appropriate Alexa Fluor 546–conjugated (catalog A11018) or A488-conjugated (catalog A11070) goat anti-mouse or anti-rabbit antibody (Thermo Fisher Scientific). After a final wash with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were then incubated in appropriate secondary antibodies (goat anti-rabbit-HRP, Invitrogen G21234, 1:10,000; goat anti-mouse IgG1, Invitrogen PA174421, 1:5,000) for 30 minutes at room temperature before washing and enhanced chemiluminescence with (SuperSignal™ West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Biochemistry 2022Quote: ... Two milligrams of total protein were incubated overnight at 4°C with Pierce Protein G magnetic beads (ThermoFisher), followed by successive washes ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were stored at −80°C for later protein extraction (TPER Tissue Protein Extraction Reagent (Thermo Scientific: 78510) + HALT (1x)) ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants (2 mg protein) were removed and incubated overnight at 4°C with Dynabeads Protein G (Thermo Scientific) coated with anti-FLAG antibody or IgG heavy chain according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Protein-DNA-antibody complexes were then precipitated with Dynabeads Protein G (Thermofisher 10003D) or Dynabeads MyOne Streptavidin C1 (Thermofisher 65001 ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibody-bound chromatin was purified using protein A and protein G Dynabeads (Invitrogen) followed by two washing steps with increasing concentration of NaCl and one washing step with TE buffer as described before (12) ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody-bound proteins were captured with Dynabeads Protein A (Thermo Fisher Scientific) for 2 hr at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Protein digestion was performed overnight at 37°C with trypsin/Lys-C protease mix (cat# A40009, Thermo Fisher Scientific) in 125 µL 50 mM TEAB (1:30 enzyme:protein ratio) ...
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bioRxiv - Biochemistry 2023Quote: ... and the antibody was c-Myc monoclonal antibody 9E10 (Life Technologies, catalog number 132500).
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bioRxiv - Microbiology 2021Quote: ... Antibodies to viral proteins were directly conjugated using antibody labeling kits (ThermoFisher). For viability staining ...
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bioRxiv - Microbiology 2020Quote: ... or P1 SARS-CoV-2/München- 1.1/2020/929 (Munich) virus was diluted to 500 µl in 15 ml with virus dilution medium (Opti-MEM, Gibco) supplemented with 2% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Next day cells were washed with phosphate-buffered saline (PBS) and infected with virus in Virus Production Serum Free Media (VPSFM; Gibco) supplemented with 0.25-0.5 ug/mL TPCK-Trypsin ...
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bioRxiv - Biochemistry 2024Quote: ... Ten micrograms of total RNA was reverse transcribed (RT) at 37 °C using random hexamer primers and Moloney murine leukemia virus retrotranscriptase (Invitrogen-Life Technologies, Buenos Aires, Argentina) in a 20 µL reaction mixture ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was incubated at 4°C for 1 h with anti-BmAgo3 antibody23 conjugated to Dynabeads protein G (Thermo Fisher Scientific). The superparamagnetic beads were washed five times with wash buffer (30 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 mins at 4°C and resulting supernatants were incubated with 50 μL of anti-HA magnetic beads or protein A magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C with rotation (10 rpm) ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of lysates were incubated with anti-53BP1 for 2 hours at 4°C and subsequently with Protein A magnetic beads (Thermo Fisher Scientific) for 1 hour at 4 °C ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488 anti-mouse IgG2b, Alex Fluor 555 anti-mouse IgG2a, or Alex Fluor 647 anti-rabbit IgG; 1:1,000, Invitrogen). Slides were observed using an LSM 710 Zeiss confocal laser microscope ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-TRF1 antibody (Goat Pab sc-1977, SantaCruz), or anti-PAR (Mouse Mab 10H ALX-804220, Alexis) recovered with Protein-G dynabeads (Invitrogen), run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins ...
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bioRxiv - Systems Biology 2020Quote: ... Secondary detection of bait proteins was performed in 2.5% bovine serum albumin (BSA) in PBS using goat anti-mouse Alexa FluorTM 488 (Molecular Probes, ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD proteins were either precomplexed with StrepMAB-Classic HRP (IBA) and secondary goat anti-mouse IgG Alexa Fluor 555 (Invitrogen) in a 4:2:1 molar ratio or conjugated with pA-LS NP in a 1:1 molar ratio ...
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bioRxiv - Physiology 2022Quote: ... plasma C-reactive protein (CRP) was measured using an ELISA (Invitrogen, EM20RB). The ELISA was performed according to the manufactures protocol and the results were measured using a SpectraMax iD3 microplate reader (OD 450 nm) ...
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bioRxiv - Immunology 2019Quote: ... and Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen). Amplification of cDNAs was performed by quantitative real-time PCR reactions on a Light Cycler instrument (LC480 ...
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bioRxiv - Microbiology 2020Quote: ... Virus was diluted in infection media (DMEM (Invitrogen) supplemented with 35% BSA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... using Sendai virus-mediated transduction (Thermofisher Scientific, #A16517) or nucleofection (Amaxa ...
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bioRxiv - Microbiology 2020Quote: ... and 50% virus solution in RPMI media (Gibco). Mosquitoes were left to feed for 1.5 h using Hemotek membrane feeder system (Discovery Workshops ...
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bioRxiv - Immunology 2021Quote: ... virus was diluted in RPMI-1640 media (Gibco) and maintained on ice ...
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bioRxiv - Neuroscience 2022Quote: ... virus was mixed with 4% fast green (Invitrogen) dissolved in saline and injected using a glass micropipette at a rate of 100 nl/min ...
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bioRxiv - Genetics 2024Quote: ... using Sendai virus-mediated transduction (Thermofisher Scientific, #A16517) or nucleofection (Amaxa ...
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bioRxiv - Neuroscience 2023Quote: ... Virus particles were resuspended in Neurobasal A (Invitrogen) and stored at -80 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... then virus pellets were suspended in HBSS (Gibco). The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara) ...
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bioRxiv - Genomics 2023Quote: ... infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... or Protein G (Thermo Fisher Scientific, Cat. #10002D, for mouse). HEK293T lysates were supplemented with 1.5 mM CaCl2 and 1 μM calmodulin (final concentrations ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding mouse PRMT proteins were subcloned into pFASTBAC1 (Invitrogen) with a FLAG tag ...
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Plant Biology 2019Quote: ... 10 μL of anti-GFP antibody (MBL-598) and 50 μL of Dynabeads Protein G (Thermo Fisher Scientific) were used ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Protein products with HA tag were detected using 1:500 anti-HA antibody (Life Technologies, Catalog number: 326700), NURF-1.D isoform with FLAG tag was detected using 1:1000 PIERCE ANTI-DYKDDDDK antibody (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and the proteins analyzed by Western blotting using 1:2,000 goat anti-gp120 polyclonal antibody (Thermo Fisher Scientific) and 1:2,000 HRP-conjugated rabbit anti-goat IgG (Thermo Fisher Scientific ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... The leftover samples were precipitated with anti-FLAG antibody which was prebound to Protein G Dynabeads (Thermo Fisher). The 50 µL of saved chromatin and immunoprecipitated DNA were de-crosslinked in a 65°C water bath overnight ...
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bioRxiv - Microbiology 2020Quote: ... PhoU2-HA proteins were detected with an anti-HA rabbit polyclonal antibody (Invitrogen, 71-5500, 1:500 dilution) as primary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... With the following exceptions: the nitrocellulose-coated coverslips were incubated with protein or with anti-GST antibody (Invitrogen) for 4 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Cbf11-TAP protein was verified by western blot with an anti-TAP antibody (Thermo Scientific, CAB1001). Prototrophic cbf11-TAP strain was then prepared by standard crossing and revalidated.