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Citations for Thermo Fisher :
3951 - 4000 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated with both WA2.22 Fc fusion (100 nM) and AT8 (1:200 dilution, Invitrogen) in M ...
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bioRxiv - Bioengineering 2023Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:10,000 ...
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bioRxiv - Immunology 2022Quote: ... S.Tm were washed with PBS and reconstituted in DMEM/F-12 (STEMCELL) supplemented with 3% FCS (Thermo Fisher). For 2D enteroid-derived monolayer infections ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Biowest Riverside) and 1% penicillin/streptomycin (Thermo Fisher Scientific), after incubation with Fc receptor blocking reagent (Miltenyi Biotech) ...
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bioRxiv - Microbiology 2023Quote: Cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... followed by three washes with 1% FCS/PBS and secondary staining using Alexa-546-anti-rabbit (Invitrogen, A11035), Alexa-488-anti-mouse (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), Epcam-BV421 (clone G8.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... were grown in DMEM (Dulbecco’s Modified Eagle’s Medium, Hyclone) supplemented with 10% (v/v) FCS (Fetal Calf Serum, Gibco), 3.7 g/l sodium bicarbonate ...
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bioRxiv - Cancer Biology 2023Quote: Viably frozen samples obtained from patients diagnosed with AML or normal CD34+ hematopoietic progenitor cells were thawed in 37°C water bath as fast as possible and placed immediately in 20% FCS Iscove’s Modified Dulbecco’s Medium (Gibco IMDM ...
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bioRxiv - Cancer Biology 2023Quote: ... suspended in 20μl sterile complete media (MEM-Alpha (Biological Industries)) supplemented with 10% Fetal Calf Serum (FCS) (Gibco) and 1% L-Glutamine/Penicillin/Streptomycin (Biological Industries) ...
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bioRxiv - Immunology 2024Quote: ... and absorbance was measured at 450 nm and 570 nm using the Multiskan FC Microplate Photometer (Thermo Scientific). To correct for optical imperfections ...
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bioRxiv - Molecular Biology 2024Quote: In order to block non-specific Fc-mediated interaction,1 μg of normal Rabbit IgG (ThermoFisher Cat: 31235) is added to the cell sample and incubated for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... and the absorbance at 750 nm was measured using a plate reader (Multiskan FC, ThermoFisher, Waltham, MA, USA). Total polyphenols content was expressed as gallic acid equivalent per gram of watermelon flesh sample.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAX [Gibco] supplemented with 5% fetal bovine calf serum (FCS; [Gibco]) and 50 μg/mL gentamicin [Gibco] ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463 ...
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bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: The human anti-DNP and anti-WTA GlcNAc-β-4497 monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (21) ...
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bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
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bioRxiv - Bioengineering 2020Quote: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041).
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bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: Silencer Select siRNA negative control (4390844) and those targeting GFAT1 (human, s5708; mouse, s66610) or GFAT2 (human, s19305) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... Other monoclonal human anti-DNP and anti-WTA IgG of the four IgG subclasses were produced recombinantly in human Expi293F cells (Life Technologies) as described before 51 with minor changes ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell binding experiment was done with the human T lymphocyte cell Jurkat and anti-human IgG antibody-Ax488 (ThermoFisher Scientific). Ax488 signal changes upon binding were detected using a flow cytometer (BD Accuri C6 ...
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bioRxiv - Cell Biology 2023Quote: HCT116 human colorectal cancer and SUIT-2 human pancreatic ductal adenocarcinoma cells were grown in McCoy’s 5A medium (Gibco, Cat. # 26600080) supplemented with 10% v/v dialyzed Foetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... Human serum used for all conditions was from the same lot of pooled human AB serum (Fisher Scientific, Cat#: BP2525-100). For heat inactivation ...
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bioRxiv - Cell Biology 2024Quote: HepG2 (Human hepatoma cell line, ATCC, Manassas, Virginia, United States of America) and HEK293A (Human embryonic kidney cell line, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.
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bioRxiv - Cell Biology 2021Quote: ... the cell number was determined by manually counting of 10×13 tile images (4X) using Fiji Software.27 Cells were harvested using TrypLE™ Express Enzyme (Gibco, Thermo Fisher Scientific) and subcultured in the same conditions ...
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bioRxiv - Molecular Biology 2022Quote: INS-1E or INS-1 832/13 cells were seeded in a 12-well plate and transfected with siRNA using RNAiMAX (Thermo Fisher Scientific, Cat# 13778150). ON-TARGET plus SMARTpool siRNA was used for knocking down rat Wfs1 (Dharmacon ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... The enriched cells were then subjected to magnetic bead separation (EasySep Mouse Streptavidin Rapidspheres Isolation Kit, Stem Cell, 19860) to remove the EpCAM+ cells (CD326 1:200, ThermoFisher Scientific, 13-5791-82). The EpCAM-depleted cells were stained with antibodies against CD45 and a viability dye ...
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bioRxiv - Molecular Biology 2024Quote: ... EpCam+ epithelail cells were isolated by EasySep™ Release Mouse Biotin Positive Selection Kit according to manufacturer protocol and EpCAM ab (Invitrogen 13-5791-82).
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bioRxiv - Neuroscience 2024Quote: ... The capillary was then filled with AAV8-CMV.III.eGFP-Cre.WPRE.SV40 with a titer of 1.00E+13 (Penn Vector Core, University of Pennsylvania) diluted 1:10 in PBS with Fast Green FCF (Fisher Scientific, Cat. No. BP123-10) for visualization (1.00E+12 final titer after dilution) ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were diluted in 1 % non-fat dry milk in TBS-T: mouse anti-TFR1 antibody (1:1,000, Thermo Fisher Scientific, 13-6800, RRID:AB_2533029), rabbit anti-FPN antibody (1:2,000 ...
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bioRxiv - Plant Biology 2024Quote: ... a pH reading was collected for each well using a Fisher accumet excel XL 25 dual channel pH/ion meter (13-636-XL25; Fisher Scientific, Waltham, MA, USA) and a Fisher accumet microglass ...
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions of human PBMC were stimulated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 0.01ug/ml soluble human anti-CD3 (R&D clone UCHT1) and 0.1 ug/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) at a concentration of 1×106 cells/ml in RPMI-1640 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y human neuroblastoma (ECACC) and RH-30 human rhabdomyosarcoma (kindly donated by Marc-David Ruepp) were maintained in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Cell lines used in this study were THP-1 (human monocytic leukemia) cells, HeLa (Henrietta Lacks, human cervical carcinoma cells, CCL2) and HEK293T Flp-In T-REx 293 (Thermo Fisher Scientific). THP-1 cells were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Immunology 2021Quote: ... Human primary CD8+ T Cells were negatively isolated from PBMCs using Dynabeads™ Untouched™ Human CD8 T Cells Kit (ThermoFisher Scientific) or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec) ...