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Citations for Thermo Fisher :
3951 - 4000 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... enrichment and chip loading was performed on an Ion Proton Chef system using the Ion PI™ Hi-Q™ Chef Kits (ThermoFisher Scientific); sequencing was performed on an Ion Proton PI™ V3 chip ...
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bioRxiv - Immunology 2022Quote: ... Human PBMCs and C57BL/6 mouse PBMCs not immediately used in any experiments were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Cell Biology 2020Quote: ... purified His-calmodulin was dialyzed in a Spectra/Por™ 4 RC Dialysis Membrane Tubing (08-667D, Thermo Fisher Scientific, Waltham, MA, USA) against 1,000 volumes of PBS supplemented with 0.5 mM EGTA at 4 °C for 4 h and replaced with fresh 1,000 volumes of PBS twice.
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Molecular Biology 2024Quote: ... were amplified from differentiated P19 mouse embryonal carcinoma cells15 and ligated into the multiple cloning site of the expression vector pcDNA3.1 myc-His B (Thermo Fisher Scientific; Waltham, MA). Snapin was cloned in-frame with the downstream myc-His tags ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Genetics 2024Quote: ... Membrane was stripped using PVDF stripping solution (DOT Scientific 65000-500) according to manufacturer instructions and reprobed with anti-6x-His primary antibody (Thermo Fisher MA1-21315) and conducted identically to the first probing ...
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bioRxiv - Neuroscience 2023Quote: An PPP2R2B-AS1 fragment containing the region 44 bp upstream of the CTG repeat and 145 bp downstream from the repeat was PCR-amplified and cloned into pcDNA3.1(-) myc-his A vector (Life Technologies, Grand Island, NY) at EcoRV site ...
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bioRxiv - Immunology 2023Quote: ... The resulting libraries were multiplexed and sequenced with Ion Proton technology and Ion Hi-Q chemistry (Ion Torrent, Thermo Fisher Scientific, Vienna, Austria). CUTADAPT 4.0 was used to trim sequencing reads of low-quality bases and poly-A tails with the options -q 20 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pull-down assay follows the protocol provided by Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA). The fluorescence intensity (excitation = 485 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
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bioRxiv - Cell Biology 2023Quote: Primary antibodies used in western blotting (WB) and immunofluorescence (IF) were anti-6x His mouse monoclonal (Invitrogen, #MA1-21315, 1:5000 in WB), anti-GST mouse monoclonal (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... to digest the phosphorylated OsPrx20-His and analyzed by the LC-MS/MS system (LTQ Orbitrap XL ETD; ThermoFisher Scientific, Waltham, MA, USA) as described previously (Gampala et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... Green fluorescent protein (GFP) standard curve was created from dilutions of Aequorea Victoria GFP His-tag recombinant protein (ThermoFisher Scientific, Cat. No. A42613) and measured on the BioTek Cytation5 Imaging Reader ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2020Quote: ... Reverse-phase chromatography was performed with an Ultimate 3000 Nano LC System (Thermo Fisher Scientific, Rockwell, IL, USA), which was coupled to a Q Exactive HF mass spectrometer (Q ExactiveTM HF Hybrid Quadrupole-OrbitrapTM Mass spectrometer ...
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bioRxiv - Cancer Biology 2021Quote: ... and the protein concentration for each western blot was specified via BCA assay (Thermo Scientific, Rockford, IL, USA). Each sample was composed of equal amounts of protein in addition to varying amounts of sodium dodecyl sulfate buffer (SDS sample buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... After adding ∼100ul of secondary antibodies diluted in 1% BSA+Triton-X (Thermo Fisher Scientific, Rockford, IL, USA), the sections were incubated for 2.5 hours at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... resolved on 4-20% gradient gels and stained overnight with Gelcode blue stain (Thermo Fisher Scientific, Rockford, Il). Five bands were cut out spanning the length of the lane and sent for protein mass spectrometry analysis.
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bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
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bioRxiv - Molecular Biology 2021Quote: The peptide concentration was determined by using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, IL) and 180 µg peptides from each sample were loaded onto MacroSpin column (NestGroup ...
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bioRxiv - Immunology 2021Quote: ... IL-17A gene expression levels were measured by quantitative RT-PCR with Power SYBR Green technology (Applied Biosystems). Probes were obtained from Applied Biosystems as Assays-on-Demand™ Gene Expression Assays (glyceraldehyde-3-phosphate dehydrogenase GAPDH ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce, Rockford, IL, USA) and analyzed by 10% SDS-PAGE with Coomassie blue staining.
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bioRxiv - Plant Biology 2020Quote: ... followed by addition of 100× diluted secondary antibody: FITC-linked anti-rat IgG (Thermo Fisher Scientific, Rockford, IL) for an additional 1 h ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The concentrations of protein in homogenate samples were determined using Pierce BCA protein assay (Thermo Scientific, Rockford, IL).
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bioRxiv - Cell Biology 2021Quote: ... by flow cytometry using an anti-pan cytokeratin AE1/AE3 monoclonal antibody (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cancer Biology 2021Quote: ... The final detection was performed by enhanced chemiluminescence with SuperSignal West Femto reagent (Thermo Scientific, Rockford, IL, USA). Equal protein loading was confirmed by stripping the blot and re-probing it with 1:1,000 anti-actin antibody.
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bioRxiv - Biochemistry 2020Quote: ... The antigen-antibody complexes were detected using the Pierce ECL chemi-luminescent detection system (Thermo Scientific, Rockford, IL) and visualized on the ChemiDoc XRS+ gel documentation system (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... The HCT116 cells were grown in the Lab-Tek Chambered Coverglass System (154526, Thermo Scientific, Rockford, IL, USA), and the cells were then incubated with 100 nM LysoTracker Deep Red Probe (L12492 ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein concentration for each western blot sample was determined via BSA assay (Thermo Scientific, Rockford, IL, USA). Samples were then equalized and 6x sodium dodecyl sulfate buffer (SDS sample buffer ...
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bioRxiv - Immunology 2022Quote: Cytokine concentrations in culture supernatants were determined using specific ELISA kits (IL-1β and TNFα, Thermo Fisher Scientific) according to instructions provided by the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... Normal body temperature (36-37°C) was maintained with a circulating warm water blanket (Thermo Scientific, Rockford, IL).
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bioRxiv - Neuroscience 2022Quote: ... and fractionated using the Pierce High-pH Reversed-Phase Peptide Fractionation (HPRP) Kit (Thermo Fisher Scientific, Rockford, IL) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were homogenized for approximately 45 s with a polytron homogenizer (Powergen, Fisher Scientific, and Hanover Park, IL). Throughout homogenization ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by growth in the absence of IL-2 for 6 - 9 h in RPMI 1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... These pieces were digested with 10 ml HBSS containing 2 mg/mL collagenase I (Thermo Scientific, Rockford, IL) for 30 minutes at 37°C with gentle stirring and supernatants were sieved through a 100-μm nylon mesh ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were measured on a quadrupole Orbitrap mass spectrometer (Q Exactive HF, Thermo Fisher Scientific, Rockford, IL, USA) coupled to an EASYnLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2020Quote: ... T4 DNA Ligase and JetFlex™ Genomic DNA Purification Kit was obtained from Invitrogen (Thermo Scientific, IL, America). KOD DNA polymerase was purchased from Toyobo ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes, and fixed with methanol:glacial acetic acid, (5:1, v/v) (FisherThermo Fisher Scientific, Rockford, IL). Cells were dropped onto slides ...
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bioRxiv - Biochemistry 2021Quote: ... Protein content was determined by the micro bicinchoninic acid protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA).