Labshake search
Citations for Thermo Fisher :
3951 - 4000 of 5131 citations for FADD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: Peptide/Protein identification and MS1-based label-free quantification were carried out using Proteome Discoverer 2.5 (PD2.5) (Thermo Fisher Scientific) or Skyline version 22.2 (MacCoss lab software) ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteolytic peptides were finally concentrated in a trapping column (C18 Pepmap, 300 μm i.d. × 5 mm length, Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2023Quote: ... Samples were then separated into 8 fractions using the High pH reversed-phase peptide Fractionation Kit (ThermoFisher Scientific, cat# 84868). The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... Non-deuterated samples peptide identification was performed via data dependent tandem MS/MS experiments and analyzed by Proteome Discoverer 2.5 (Thermo Fisher). Mass analysis of the peptide centroids was carried by HDExaminer v3.3 (Sierra Analytics ...
-
bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped in a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap 100, Thermo Fisher Scientific) with buffer A (2/98 MeCN:H2O in 0.1% formic acid ...
-
bioRxiv - Genetics 2023Quote: ... Peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific, Waltham, MA) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
-
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: TMT-labeled peptides were analyzed using an EASY-nLC 1200 UPLC coupled with an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). LC buffer A (0.1% formic acid ...
-
bioRxiv - Biochemistry 2023Quote: ... MS data of the peptides were acquired on a Q Exactive mass spectrometer coupled to EASY-nLC (Thermo Fisher Scientific) using a data-dependent top 10 methods that dynamically choose the most abundant precursor ions from the survey scan (300–1800 m/z ...
-
bioRxiv - Molecular Biology 2022Quote: ... Peptides were desalted with an Oasis PRiME HKB μElution Plate and analysed on a QExactive HF HPLC-MS (Thermo Scientific). Separation by HPLC prior to MS occurred on an Easy-nLC1200 system (Thermo Scientific ...
-
bioRxiv - Physiology 2023Quote: ... the labeled sample was separated into five fractions using the high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Bound peptides were eluted with 1 ...
-
bioRxiv - Biochemistry 2023Quote: We acidified the digested samples with concentrated trifluoroacetic acid (TFA) and cleaned up the peptides with C18 SOLA Solid-Phase Extraction (SPE) columns (ThermoFisher). Briefly ...
-
bioRxiv - Cell Biology 2023Quote: ... The peptides were further separated onto an Easy-Spray RSLC C18 column (75 µm i.d., 50 cm length, ThermoFisher Scientific) using a 30 min linear gradient (15% to 35% solvent B (0.1% formic acid in acetonitrile) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Peptides were loaded onto a trapping cartridge (Acclaim PepMap C18 100Å, 5 mm x 300 µm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN ...
-
bioRxiv - Microbiology 2023Quote: ... and peptide concentrations for each sample were estimated based on absorbance measurements at 280 nm (NanoDrop™, Thermo Fisher Scientific). For each sample ...
-
bioRxiv - Immunology 2022Quote: Peptide separation in buffer A (0.1% formic acid) was achieved using an EASY-nLC 1200 HPLC system (Thermo Fisher Scientific) and a column (75 µm inner diameter ...
-
bioRxiv - Biochemistry 2023Quote: ... 20 μg of peptides from each sample were reacted for 1 h with the appropriate TMT-NHS isobaric reagent (ThermoFisher) in 40% (v/v ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were washed with warmed PBS before the addition of peptide in DMEM (without phenol red) with 10% FBS (ThermoFisher) for 2-4 hours at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... The resulting peptides were analyzed by a top-15 data dependent LC-MS/MS strategy using a QExactive plus mass spectrometer (ThermoFisher). Briefly ...
-
bioRxiv - Developmental Biology 2023Quote: ... Peptides were injected on a PepMap 100 C18 trap column (100 µm × 2 cm, 5 µM particles, Thermo Fisher Scientific) and separated with an EASY-Spray analytical column (PepMap RSLC C18 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Final peptide concentration was measured by absorbance at 280 nm on a Nanodrop ND-2000 spectrometer (Thermo Fisher, Waltham, MA).
-
bioRxiv - Biochemistry 2023Quote: ... Each sample of peptides was loaded onto a C18 trap column (75 μm ID × 2 cm, 3 μm, Thermo Scientific) and then separated on a C18 analytical column (75 μm ID×50 cm ...
-
bioRxiv - Cancer Biology 2023Quote: ... and digested tryptic peptides were analyzed by LC-MS/MS on Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific) using the same configuration and settings as previously reported85 ...
-
bioRxiv - Biochemistry 2023Quote: ... and applied to TiO2 spin tips (3 mg peptides per tip) for phosphopeptide enrichment according to the manufacturer’s protocol (High-SelectTM TiO2 Phosphopeptide Enrichment Kit, A32993, Thermo Scientific). Eluted phosphopeptides were transferred to Lo-bind Eppendorf tubes ...
-
bioRxiv - Biochemistry 2023Quote: ... endoprotease Lys-C and Trypsin digested peptides were analyzed on an LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Bremen). The raw data were analyzed with MaxQuant (version 2.0.3.0) ...
-
bioRxiv - Microbiology 2023Quote: The enriched and unenriched samples were fractionated with the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer instructions for TMT-labeled peptides ...
-
bioRxiv - Cell Biology 2023Quote: ... Approximately 25 µg of peptides for each sample was labeled with 0.2 mg of TMT-10plex reagents (Thermo Fisher Scientific) for 1 h at 25 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50 cm × 75 μm ID; Thermo Fisher Scientific). Using a constant flow rate of 300 nL/min ...
-
bioRxiv - Molecular Biology 2023Quote: ... cDNA encoding the mature protein (without the signal peptide) was amplified using gene-specific primers incorporating restriction enzyme sites and subcloned into the pPICZα A vector (Invitrogen) using EcoRI and XbaI enzymes and T4 ligase (Thermo Fisher Scientific) ...
-
bioRxiv - Physiology 2023Quote: ... 1 µg peptides were injected on an Ultimate 3500 RSLC coupled to a Q-Exactive HF-X (Thermo Fisher Scientific). Peptides were first trapped using a PepMap 100 C18 trap column and then separated with a PepMap C18 analytical column (75 µm x 50 cm ...
-
bioRxiv - Physiology 2023Quote: ... with peptides introduced via an EasySpray Nano source coupled to a Q-Exactive Plus Quadrupole Orbitrap mass spectrometer (Thermo Scientific) and subjected to data dependent tandem mass spectrometry (MS/MS) ...
-
bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...
-
bioRxiv - Neuroscience 2023Quote: The peptides were analysed using liquid chromatography/mass spectrometry (MS) using with an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) coupled to an UltiMate3000 RSLCnano LC system (Dionex Co. ...
-
bioRxiv - Molecular Biology 2023Quote: ... Tryptic peptides were analyzed with a Vanquish Neo UHPLC system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Peptides were separated using an increasing acetonitrile gradient from 2% to 25% over 80 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... Resulting tryptic peptides were separated by reverse-phase high-performance liquid chromatography (RP-HPLC; UltimateTM 3000 RSLCnano system; Thermo Scientific) using a 50 cm analytical column (in-house packed with Poroshell 120 EC-C18 ...
-
bioRxiv - Immunology 2023Quote: ... One microgram of peptide sample was loaded on a C18 column (15 cm, 3.0 μm Acclaim PepMap, Thermo Fisher Scientific), separated with a 0–40% gradient of buffer B (80% acetonitrile ...
-
bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a PepMap C18 trap column (300 µm x 5 mm, 5 µm particle size, Thermo Fisher) and separated on a 50 cm Easy-Spray column (ES803 ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was transferred to a new tube and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84688) following manufacturer’s recommendation.
-
bioRxiv - Cancer Biology 2023Quote: ... The eluting peptides were ionized at a potential of + 2 kVa into a Q Exactive mass spectrometer (Thermo Fisher, Germany). Intact masses were measured at resolution 70.000 (at m/z 200 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sep-Pak Vac tC18 solid phase extraction cartridges (Waters) and the concentration determined using the Pierce quantitative colorimetric peptide assay (ThermoFisher). Peptides were dried by vacuum centrifugation.
-
bioRxiv - Biochemistry 2023Quote: ... We analyzed MS/MS data collected from ND samples to identify the likely sequence of the patent peptides using SEQUEST (Bioworks v3.3.1, Thermo Fisher Scientific) with a peptide tolerance of 8 ppm and a fragment tolerance of 0.1 AMU ...
-
bioRxiv - Cell Biology 2024Quote: ... The digested peptides were then desalted using Pierce C18 Spin Tips & Columns system according to the manufacturer’s instructions (ThermoFisher Scientific). Three independent biological replicates for each condition were analyzed by mass spectrometry.
-
bioRxiv - Neuroscience 2024Quote: ... Peptides were first trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
-
bioRxiv - Biochemistry 2024Quote: Enriched crosslinked peptides were injected onto a C18 PepMap100-trapping column (0.3 x 5 mm, 5 μm, Thermo Scientific™) connected to an in-house packed C18 analytical column (75 μm x 300 mm ...
-
bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed on a EASYspray C18 column (50 cm x 75 um, Thermo Fisher Scientific, San Jose, CA) over a 65-minute gradient at a flow rate of 250 nL/min ...
-
bioRxiv - Cancer Biology 2024Quote: T cells from KOG mouse TDLNs (5 × 105) were co-cultured with SIINFEKL peptide (1 μg/mL, Thermo Fisher Scientific) in a 96-well plate (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2024Quote: ... peptides were separated on a 75 µm ID x 25 cm C18 Acclaim PepMap® RSLC column (Thermo Scientific, USA) with a 4-40% linear gradient of solvent B (0.1% formic acid in 80% ACN ...
-
bioRxiv - Biochemistry 2024Quote: The tryptic peptides were injected onto a pre-column (PepMap C18, 5 mm × 300 μm × 5 μm, Thermo Fisher Scientific) and separated ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting peptides were desalted using Sep-Pak C18 cartridges (Waters, MA, USA) and dried in a SpeedVac (Thermo Scientific).
-
bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched in 0.5% trifluoroacetic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/μL ...
-
bioRxiv - Biochemistry 2024Quote: ... and the resulting peptides were analysed by LC-MSMS on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) using CID+ETD activation ...