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Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for Anti ChAT SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... RNA was extracted using the RNeasy micro extraction kit and reverse transcribed it to generate cDNA using a standard kit following the manufacturer’s instructions (Superscript III, Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 100 mg of ground tissue per sample was extracted using the Kit Pure link RNA Mini Kit (Ambion) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The elastase levels within the supernatant were measured using an Elastase Assay kit (EnzChek Elastase Assay kit, Life Technologies) according to manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: Plasmids for microinjection were extracted using the PureLink HQ Mini Plasmid Purification Kit or HiPure Plasmid Midiprep kit (Invitrogen). Genomic DNAs were extracted from mix-staged animals with PureLink Genomic DNA Mini Kit (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... DNA concentration was assessed with Qubit broad range kit (Qubit™ dsDNA BR Assay Kit, Q32850, Thermo Fisher Scientific) or nanodrop ...
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bioRxiv - Genetics 2020Quote: ... and mRNA was synthesized and purified using an In Vitro RNA Transcription Kit (mMESSAGE mMACHINE T7 Ultra kit, Ambion). SgRNA oligos were amplified and transcribed in vitro using the GeneArt Precision gRNA Synthesis Kit (Thermo ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Pierce™ LDH Cytotoxicity Assay Kit and Amplex™ Red Cholesterol Assay Kit were purchased from Invitrogen (Waltham, MA). 96-well black microplates were purchased from Greiner bio-one (Kremsmünster ...
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Modeling PPRV pathogenesis in mice to assess the contribution of innate cells and anti-viral T cellsbioRxiv - Immunology 2020Quote: ... The mRNA was converted into cDNA using a first strand synthesis kit (Verso cDNA synthesis kit, Thermo Fisher Scientific). The expression of hypoxanthine phosphoribosyltransferase 1 (HPRT 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... following the instructions provided in the kit Click-IT EdU Alexa Fluor 488 Flow Cytometry Assay kit (Thermo Fisher). For DNA damage analysis ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were measured using the Bio-Rad Protein Assay Kit or BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts (25 μg or 10 μg ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was quantified using the “Protein Quantification BCA Assay” kit (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined with the MicroBCA Assay kit (Pierce BCA protein estimation kit, 23225, ThermoFisher Scientific, United Kingdom).
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bioRxiv - Microbiology 2024Quote: ... Sequencing cleanup was done using the QIAquick PCR Purification Kit followed by quantitation using the Qubit dsDNA HS Assay Kit on the Qubit 2.0 Fluorometer from Invitrogen by Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... kit and cDNA was synthesized using the SuperScript™ III Reverse Transcriptase kit (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of the DNA was measured using a QUBIT kit 1x dsDNA HS Assay kit (ThermoFisher, Waltham, MA) and fragment size was assessed on a Femto Pulse (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... Membrane and cytosolic fractions were isolated using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific kit #89842).
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bioRxiv - Molecular Biology 2023Quote: ... cells were permeabilized with 1X permeabilization buffer (PB) (kit Foxp3/Transcription Factor Staining Buffer kit, Thermo Fisher, 00-5523) for 30 min RT ...
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bioRxiv - Neuroscience 2023Quote: ... Amyloid Aβ40 and Aβ42 in the hippocampus and striatum and D2R in the striatum were quantified by ELISA with the following kits: Aβ40 human ELISA kit (Thermofisher); Aβ42 human ultrasensitive ELISA Kit (Thermofisher) ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the protein was estimated using the BCA kit (Pierce BCA Protein Assay Kit - Thermo Fisher Scientific) according to the supplier’s recommendations as well as by comparing the protein on a 12.5% acrylamide Coomassie-stained SDS-PAGE gel with BSA standards ...
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bioRxiv - Cell Biology 2024Quote: ... Amplified DNA/ cDNA was then purified with MicroCLEAN Kit (Clent Life Science) and further processed with the BigDye Terminator v1.1 Cycle Sequencing Kit (ThermoFisher). Sequencing was performed with the ABI PRISM 3730 DNA Analyzer (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time RT-PCR was performed using a reverse transcription kit and real-time PCR kit (Applied Biosystems, USA) with three replicates ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time RT-PCR was performed using a reverse transcription kit and real-time PCR kit (Applied Biosystems, USA). The following primers were used.
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was purified using GeneJet Plasmid Miniprep kit (Thermo) or the Purelink HiPure Plasmid Midiprep kit (Invitrogen K210005). PCR was performed according to manufacturer directions using Q5 ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs isolated from healthy donors were stimulated for 24h using anti-CD3/anti-CD28 antibody-coated beads (Invitrogen) at a bead ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used were goat anti-rabbit Alexa 488 and goat anti-mouse Alexa 546 (1:200, Invitrogen). Images were acquired using a Leica SP5 II Confocal microscope under 25x objective ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor donkey anti-sheep 488 secondary or Alexa Fluor donkey anti-rabbit 594 secondary (Thermo Fisher Scientific) diluted (1:500 ...
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bioRxiv - Immunology 2021Quote: ... goat anti-rabbit (11010, Alexa Fluor 564) and goat anti-mouse (21242, Alexa Fluor 647) from Thermo Fisher, Massachusetts ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with goat anti-mouse IgG AF555 and goat anti-rabbit IgG AF488 (Invitrogen Life Technologies). DNA was counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with goat anti-mouse IgG AF555 and goat anti-rabbit IgG AF488 (Invitrogen Life Technologies). DNA was counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then incubated with anti-CD11b(AF488) and anti-F4/80(PerCP/Cy5.5) fluorescently conjugated antibodies (Invitrogen) and assayed with a BD LSRII flow cytometer.
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bioRxiv - Cancer Biology 2020Quote: ... anti-rat and anti-rabbit secondary antibodies and Alexa Fluor conjugated phalloidin were obtained from Invitrogen (Carlsbad, CA).
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bioRxiv - Developmental Biology 2021Quote: ... Goat anti-mouse and goat anti-rabbit immunoglobulin G (IgG) antibodies conjugated to Alexa Fluor 488 (Life Technologies) and diluted at 1:100 were used ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were detected by donkey anti-mouse AlexaFluor 594 and donkey anti-sheep AlexaFluor 488 antibodies (Invitrogen). For the visualization of the three-dimensional lymphatic vasculature ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies (goat anti-chicken coupled to Alexa 568; goat anti-rat coupled to Alexa 594 Invitrogen A11007), diluted 1:1000 in washing buffer ...
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bioRxiv - Immunology 2020Quote: ... the remaining 90% was incubated with anti-ThPOK antibody bound to goat anti-rabbit M280 magnetic beads (Invitrogen) overnight at 4°C prior to washing with RIPA buffer and overnight decrosslinking at 65°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary (anti-flag mouse 1:200, Cell Signalling) and secondary antibody (anti-mouse Alexa 488 1:500, Thermofisher) were diluted in blocking buffer solution and incubated overnight at 4°C or 1 hour at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary anti-mouse antibody conjugated to HRP (goat anti-mouse-HRP, 32430; ThermoFisher Scientific) at 1:20,000 in 5% milk/1xTBST for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-mouse or anti-rabbit secondary antibodies conjugated with Alexa 488 or Alexa 594 (1:500; Thermo Fisher) were incubated for 1h at room temperature Nuclei were stained with 1 μg/ml DAPI for 10 min ...
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bioRxiv - Immunology 2021Quote: ... stained with anti-cleaved caspase 3 secondary antibody (goat anti-rabbit Alexa Fluor 647, Thermo Fisher; 1:400) and anti-Ecadherin secondary antibody (goat anti-mouse Alexa Fluor 555 ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-rabbit and anti-mouse antibodies conjugated to Alexa Fluor 568 and 647 dyes (1:500; Molecular Probes) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary goat anti-mouse IgG and rabbit anti-goat IgG conjugated to horseradish peroxide (ECL; Thermo Fisher Scientific), or a fluorophore specific to the Odyssee system (IRDye 680RD and IRDye 800RD ...
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bioRxiv - Molecular Biology 2019Quote: ... and incubated with primary antibodies (anti-ZO-1, 1:50, Life Technologies; anti-occludin, 1:50, Life Technologies; anti-claudin-5 ...
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bioRxiv - Biochemistry 2019Quote: ... Staining for immunofluorescence was performed with anti-desmin or anti-α smooth muscle actin (both from Thermo Scientific), followed by goat-anti-mouse-alexa647 or donkey-a-rabbit-alexa647 (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... Rabbit anti-GFP tagged FITC (1:500) and mouse anti-Gal4 (1:200) were acquired from Thermo Scientific. Dissected larvae were mounted in Vectashield (Vector Labs ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 388 Donkey anti-Sheep IgG and Fluor 555 donkey anti-mouse IgG (all from Life technologies). For immunoblot ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa-488 donkey anti-mouse IgG and Alexa-594 donkey anti-rabbit IgG (1:500, Invitrogen, Carlsbad, CA). Immunofluorescent-labeled tissues were analyzed using a Leica DM5500 upright microscope (Leica Microsystems ...
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bioRxiv - Genetics 2020Quote: ... Alexa Fluor 488 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG from Life Technologies were used at 1:500 dilutions ...
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibody AlexaFluor647 (goat anti-mouse or goat anti-rabbit IgG, 2 ng/ml, Invitrogen, Thermo Fisher Scientific) was used at 1:200 dilution in PBS-Tween ...
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibody AlexaFluor647 (goat anti-mouse or goat anti-rabbit IgG, 2 ng/ml, Invitrogen, Thermo Fisher Scientific) was used at 1:200 dilution in PBS-Tween ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...