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Citations for Thermo Fisher :
3951 - 4000 of 8578 citations for 7 CHLOROHEPTANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: COS-7 (ATCC® CRL-1651™) cells were grown in DMEM medium (Thermo Fisher Scientific) supplemented with 10% FBS (fetal bovine serum ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: COS-7 cells were transiently transfected with the relevant DNA constructs using Lipofectamine 2000 (Invitrogen, 11668). Cell lysates (1 mg ...
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bioRxiv - Synthetic Biology 2022Quote: ... following manufacturer’s recommendations in triplicate on a QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher). Primer sequences are listed in Supplementary File 2 – Primer List.
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bioRxiv - Microbiology 2022Quote: ... 24 hours and 7 days were labelled with 0.5 μM BODIPY FL (Thermo Fisher, Waltham, MA), a hydrophobic dye that stains lipids ...
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bioRxiv - Plant Biology 2022Quote: RT-qPCR experiments were performed with three biological replicates using a QuantStudio 7 cycler (Applied Biosystems). The 10 μl reaction mixture contained 5 μl 2X SYBR Green/ROX master mix (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... The homogenate was then sonicated 10 times on setting 7 (Sonic Dismembrator Model 100, Fisher Scientific) and incubated at 4°C while rocking for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... All RT-PCR assays were done using the Viia 7 Real-time PCR System (Applied Biosystems). A standard curve was generated in order to obtain the exact number of copies in the tested sample ...
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bioRxiv - Immunology 2024Quote: ... DNA quantity was then measured using a Viia 7 Real-Time PCR System (Thermo Fisher Scientific) and the number of cycles required to achieve 1/3 of maximal SYBR Green fluorescence was determined and libraries were amplified accordingly ...
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bioRxiv - Synthetic Biology 2022Quote: ... The concentration of the transcription was measured as 7 µM using a Qubit RNA assay (ThermoFisher). A splint oligonucleotide (Z_Splint ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The RT-PCR reaction was performed using a QuantStudio™ 7 Flex system (Thermo Fisher Scientific) and data analysis was performed using the manufacturer’s web-based software (https://geneglobe.qiagen.com/analyze) ...
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bioRxiv - Molecular Biology 2022Quote: ... All qPCR analyses were performed on an Applied Biosystems QuantStudio 7 Flex Cycler (Thermo Fisher Scientific), and relative expression values were calculated using the comparative CT method (Schmittgen and Livak 2008).
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bioRxiv - Immunology 2022Quote: ... Thermal cycling and fluorescence detection were performed using the ViiA 7 real-time PCR system (ThermoFisher) according to the manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... Gene expression analyses were performed on a ViiA 7 real-time PCR system (Thermo Fisher Scientific) using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... and 300 mM NaCl – pH 7) supplemented with 160 µL of protease inhibitor cocktail (Thermo Scientific). To release the soluble protein into the media ...
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bioRxiv - Cell Biology 2024Quote: ... intracellular ROS and GSH levels in keratinocytes were measured using H2DCFDA (2’, 7’- dichlorodihydrofluorescein diacetate; Invitrogen) and CellTracker Blue (4-chloromethyl- 6.8- difluoro- 7- hydroxycoumarin ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative real-time RT-PCRs were performed in a QuantStudio 7 Flex System (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific ...
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Srs2 binding to PCNA and its sumoylation contribute to RPA antagonism during the DNA damage responsebioRxiv - Molecular Biology 2024Quote: ... to examine Srs2 level and active Rad53 form or 7% Tris-acetate gels (Thermo Fisher EA03555) to detect Rad9 phosphorylation.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The qPCR reactions were performed on a ViiA™ 7 Real-Time PCR System (Applied Biosystems) using a 96-well format ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were passaged every 7 days with collagenase type IV (Thermo Fisher: 17104019, 1.5 mg/ml) and gravitational sedimentation by washing 3 times in wash media composed of DMEM/F12 supplemented with 5% fetal bovine serum (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... for 7 min at 37°C and the digestion was blocked with 10% FBS (#10500064, Gibco) in DMEM (#41966-029 ...
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bioRxiv - Neuroscience 2024Quote: ... All qPCR reactions were run in triplicate and used SYBR-green on a QuantStudio 7 (ThermoFisher). The 2^(-ΔΔCt ...
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bioRxiv - Developmental Biology 2024Quote: ... follicles were incubated with 500 nM CellEvent™ Caspase-3/7 Green Detection Reagent (C10427, Invitrogen). Oocytes were stained with 150 nM Sir-Tubulin (CY-SC002 ...
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bioRxiv - Developmental Biology 2024Quote: ... the ovaries were sectioned at 7 μm and mounted on Superfrost Plus slides (Thermo Fisher Scientific). The slides were deparaffinized and rehydrated followed by antigen retrieval for 10 min in sodium citrate buffer (10 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification was performed in a 384 well format in a QuantStudio 7 instrument (Applied Biosystems) at the following settings ...
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bioRxiv - Biochemistry 2023Quote: ... 7 mM NaOH) followed by an incubation of 5 hrs with Dynabeads Protein G (Invitrogen, 10004D). Beads were then washed with Wash Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using the QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). The setting of the cycles used was the default for comparative Ct studies ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was carried out on a ViiA 7 Real-Time PCR System (Thermofisher Scientific), using PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... qRT-PCR were run in a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative quantification was determined using the ΔΔCt method and normalized to the endogenous controls GAPDH and RPLP0 ...
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bioRxiv - Molecular Biology 2023Quote: ... or 15 nM siRNA Control using 7 ul of Lipofectamine™ RNAiMAX transfection reagent (ThermoFisher Scientific) for each well according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was carried out using a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) and KAPA SYBR Fast qPCR reagents (KAPA Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Analysis of qPCR amplifications was performed with QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using the 2-ΔΔCT method with 36B4 serving as an internal control ...
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bioRxiv - Cancer Biology 2022Quote: ... and reactions performed on a ViiA− 7 Real-Time PCR System (all, Applied Biosystems, Waltham, MA). Data were acquired using the system’s RUO software and analyzed using the 2−ΔΔCT method ...
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bioRxiv - Developmental Biology 2023Quote: ... testicular tissues were digested with 7 ml collagenase type IV (290U/ml, Gibco cat # 17104-019) for 10 min at 37 °C with gentle agitation (250 rpm ...
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bioRxiv - Biophysics 2023Quote: ... and Septin-7 (VectorBuilder, Cat# VB220525-1339dzw) was performed in Opti-MEM (Gibco, Cat# 31985-070) using SuperFect reagent (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were run in a ViiA 7 Real-Time PCR system (Applied Biosystems, Foster City, CA) using fast reaction presets ...
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bioRxiv - Neuroscience 2023Quote: ... the hiPSCs were maintained for 7 days in a medium containing Neurobasal (Thermo Fisher Scientific, 12348017) and 1% neural induction supplement (NIS – Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... All qPCR reactions were run in triplicate and used SYBR-green on a QuantStudio 7 (ThermoFisher). The 2^(-ΔΔCt ...
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bioRxiv - Genetics 2023Quote: ... and 7 μM sections were cut using a cryostat onto Colorfrost Plus slides (Thermo Fisher Scientific). Sections were permeabilized in 0.25% Triton X-100 in PBS at room temperature for 12 min ...
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bioRxiv - Developmental Biology 2023Quote: COS-7 and HEK293 cells were grown in on plastic dishes in high-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was run in a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific 4453545) with cycle settings following manufacturer’s protocols for the RT-qPCR kit ...
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bioRxiv - Physiology 2023Quote: ... followed by 7 days of decalcification with Shandon’s TBD-2 (Thermo Fisher Scientific, Waltham, MA, USA) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split every 5-7 days by incubation with 0.5 μM EDTA (Thermo Fisher Scientific) for 4 min at room temperature and clumps were dissociated into small clumps by pipetting ...
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bioRxiv - Biochemistry 2022Quote: ... These SCX fractions (n=7) were cleaned up using C18 spin columns (89870, Pierce, ThermoFisher, USA), dried using SpeedVac at 40 °C and resuspended in formic acid (0.1 % ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT using ‘SnakeSkin’ dialysis tubing with a 7 kDa molecular weight cut-off (ThermoFisher). His6-tagged cleavage products as well as TEV protease were removed with Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... Real-time qPCR was performed in a QuantStudio 7 Flex real-time PCR system (Thermo Fisher) using iQ SYBR Green Supermix (Bio-rad #1708880 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytotoxicity of CellTrace™ Far Red-labelled tumor cells was measured by 7-AAD staining (Invitrogen) and flow cytometry ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl) containing N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM; Invitrogen) at a final concentration of 10 μM and incubated for 30 min in the dark on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Integrin αV was stained with a commercial antibody (ThermoFisher, USA, clone RMV-7, 1:1000 diluted). Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech ...
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bioRxiv - Genetics 2022Quote: ... The samples were analyzed with the ViiA 7 Real-Time PCR System and software (Applied Biosystems).
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bioRxiv - Developmental Biology 2023Quote: ... RNA and H2Omq according to the protocol on ViiA 7 Real-Time PCR System (Applied Biosystems). Primer sequences used are specified in Suppl ...