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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: PER2::LUC rhythms were monitored for 42 hours prior to the addition of 10 ng/mL of IL-1α (R&D) or matching vehicle (UltraPure DNase/RNase-Free Distilled Water, Invitrogen). Explants were heat shocked immediately or three hours after IL-1α addition.
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bioRxiv - Cancer Biology 2024Quote: ... RNA was eluted in 30µl of RNase-free water and the concentration was measured using a NanoDrop (Thermo Fisher Scientific). A total of 1µg of RNA in 20µl reaction volume was reverse transcribed using the iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... The DNA was diluted to 5 ng/µL (5x working stock) in RNase/DNase free water (Thermo Fisher Scientific #10977015) and 2 µl was loaded into a 384-well plate ...
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bioRxiv - Immunology 2024Quote: ... IFN-y+ CD3+ CD8+ T cells were sorted by FACS at 1 cell/well into 96-well PCR plates containing 15 µL of DNase/RNase-free distilled water (Invitrogen) with 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... IFN-y+ CD3+ CD8+ T cells were sorted by FACS at 1 cell/well into 96-well PCR plates containing 15ul of DNase/RNase-free distilled water (Invitrogen) with 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... and treated with RNase-free DNase (Ambion). Reverse transcription was performed from 100 to 500 ng of RNA using the Quanta qScript kit ...
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bioRxiv - Microbiology 2022Quote: ... RNase-free (1 U/µl) kit (ThermoFisher) prior to reverse transcription reaction ...
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bioRxiv - Cell Biology 2022Quote: ... RNase-free (Thermo Fisher Scientific, Cat. # EN0525), was added to 1 μg of template RNA ...
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bioRxiv - Neuroscience 2021Quote: ... RNase-free kit (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... After DNAse I RNAse-free treatment (Invitrogen) of 1 μg total RNA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... treated with RNase-free DNase I (ThermoFisher), cleaned and concentrated by phenol/chloroform extraction and precipitated with ammonium acetate and ethanol ...
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bioRxiv - Immunology 2020Quote: ... Treatment with RNase-free DNase I (Invitrogen) was used to remove contaminating genomic DNA and cDNA was prepared with the Superscript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μl RNAse-free glycogen (ThermoFisher Scientific) and 30 μl cold 100% ethanol was added ...
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bioRxiv - Neuroscience 2022Quote: ... RNAse free DNase I (Ambion™, Invitrogen) was added (0.1 mM final concentration ...
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bioRxiv - Neuroscience 2022Quote: ... RNAse free DNase I (Ambion™, Invitrogen) was added (0.1 mM final concentration ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1M Tris pH 8.0 RNase-free (Invitrogen), 5M NaCl (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... RNase-free (Thermo Scientific™, cat # EN052) for 30 min at 37 °C and then reversed transcribed with M-MLV Reverse Transcriptase (Invitrogen™ ...
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bioRxiv - Plant Biology 2023Quote: ... NaCl (5 M) RNase-free (Invitrogen, AM9759), NP40 (IGEPAL® CA-630 ...
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bioRxiv - Plant Biology 2023Quote: ... KCl (2 M) RNase-free (Invitrogen, Invitrogen), MACS® SmartStrainers (Milteny Biotec ...
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bioRxiv - Plant Biology 2023Quote: ... KCl (2 M) RNase-free (Invitrogen, Invitrogen), MACS® SmartStrainers (Milteny Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... immersed in RNAse free cold PBS (Invitrogen), and amygdalar area was dissected under a scope and immediately flash frozen in liquid nitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... RNase-free (Thermo Fisher Scientific, Cat# EN0521), following total RNA extraction ...
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bioRxiv - Plant Biology 2024Quote: ... RNase free kit (Thermo Fisher Scientific, America) following the manufacturer’s instructions cDNA synthesis was performed according to the manufacturer’s instructions using the Invitrogen SuperScript IV kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Sterile distilled water and RNAse AWAYTM (Invitrogen Corporation, USA) was used to wash sample surface and remove possible RNA-degrading contaminants.
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bioRxiv - Molecular Biology 2021Quote: ... The total yield of circular DNA was dissolved in DNase-RNase free water and then determined the concentration by Qubit fluorometer (Invitrogen, USA).
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bioRxiv - Cell Biology 2022Quote: ... was resuspended in UltraPure DNase/RNase-Free Distilled Water and transfected into cells using Lipofectamine 2000 reagent (Thermo Fisher Scientific, 11668030) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The isolated RNA was dissolved in 12 mkL of distilled RNAse-free water and reverse transcribed using miRCat-33 primer (IDT) with Superscript III Reverse Transcriptase (Life Technologies) in its buffer for 1h at 50°C ...
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bioRxiv - Microbiology 2020Quote: ... the samples were purified to obtain only the bacterial cells by first diluting them 1/4 in UltraPure™ DNase/RNase-Free distilled water (Invitrogen), then centrifuging twice at 4 226 g for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 50 ng/μl of gRNA and 200 ng/μl of single stand oligonucleotide diluted in non-DEPC RNase free water (Ambion, AM938) was centrifuged for 1 hour and 30 minutes at 15,000 rpm at 4°C and injected into the cytosol of zygotes (Volume of injection mixture ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was eluted from the columns using 30 μl of RNase-free water and quantified using a NanoDrop spectrophotometer (Thermo Scientific). cDNA was synthesized with the ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Biophysics 2020Quote: ... The mRNA pellets were resuspended in RNase-and DNase-free water and the concentration was measured using Nanodrop 2000c (Thermo Scientific). 8 M urea 10% polyacrylamide gel electrophoresis was used for mRNA quality control.
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bioRxiv - Microbiology 2022Quote: ... Total RNA was eluted in RNAse-free water and quantified using Qubit™ RNA HS Assay Kit (Thermo Fisher, USA, #Q32852). RNA integrity was assessed by non-denaturing 1.2 % (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA was eluted in RNase-free water and used for cDNA synthesis following the instructions of the Reverse Transcription Reagents Kit (Applied Biosystems) in 20 μl reactions ...
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bioRxiv - Microbiology 2020Quote: ... DNA was suspended in 30 μL RNase and DNase free molecular biology grade water and purity was assessed using a NanoDrop ND 1000 (Thermo Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... 0.5 µl of 20× primer/probe suspension and 4.5 µl of cDNA diluted in DNase/RNase-free water (10977015, Thermo Fisher Scientific). The thermocycler program consisted of an initial UDG incubation at 50°C for 2 min ...
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bioRxiv - Immunology 2022Quote: ... 1:10 dilution of the cDNA in RNAse free water was used for qPCR reaction using the SYBR green PCR master mix (Life Technologies), and reactions were performed on a Quant Studio1 sequence detection system (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... was amplified in a 20 μl reaction that contained: 11.85 μl of ultra-pure DNase/RNase free distilled water (10977023, Invitrogen, Walthan, Massachusetts); 2 μl of 10x AccuPrime PCR Buffer II (12346-086 ...
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bioRxiv - Plant Biology 2019Quote: ... and non-labeled RNAs (-EU) bound to the beads were resuspended in RNAse-free water and treated with TURBO DNase (Thermo Scientific). The DNAse was heat inactivated and reverse transcription was performed with random hexamer primers using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... 1 µM TSO (5’- AAGCAGTGGTATCAACGCAGAGTACATrGrG+G-3’, Exiqon as described in (Picelli et al., 2014) and UltraPure DNase/RNase-Free Distilled Water (Invitrogen, 10977035). The Smart-seq2 samples were centrifuged for 700 g for 10 seconds and then placed in a thermal cycler ...
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bioRxiv - Cell Biology 2019Quote: ... 1 µM IS PCR primer (5’-AAGCAGTGGTATCAACGCAGAGT-3’, IDT, as described in (Picelli et al., 2014) and UltraPure DNase/RNase-Free Distilled Water (Invitrogen, 10977035). The RNA samples were vortexed and centrifuged (700 g for 10 seconds ...
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bioRxiv - Genomics 2021Quote: ... All remaining steps followed the manufactures guidelines with elution in 30μl of RNAse-free molecular biology grade water (Thermo Fisher, U.K.).
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bioRxiv - Molecular Biology 2022Quote: ... The resulting RNA pellets were resuspended in RNAse-free water and quantified using a NanoDrop™ 2000 Spectrophotometer (Thermo Scientific™) before 10 μg of total RNA was treated with DNaseI for 15 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... After the elution with DNase/RNase free water for 2 min at RT the sample volumes were reduced using a SpeedVac (Thermo Scientific). The RNA concentration was measured using NanoDrop2000 (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... The round two PCR products were purified using AMPure XP beads following the manufacturer’s protocol and eluted in 25 μL UltraPure DNase/RNase-Free distilled water (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2023Quote: ... RNA was eluted with 20 μl of RNase-free water and yield was determined fluometrically using Qubit RNA high-sensitivity assay kit (Thermo Fisher).
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bioRxiv - Pathology 2022Quote: ... The RNA was dissolved in 30-50 μl RNase-free water and the consistency was analyzed by NanoDrop 2000 equipment (Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... RNA samples were diluted to a final concentration of 20 ng/μL with RNase-free water (Gibco®, life Technologies™). DNA contamination in the RNA samples was checked with RNase A (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... the RNA pellets were dissolved in RNase/DNase free ultrapure water and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). One microgram of RNA was converted into cDNA using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... air-dry pellets of genomic DNA were dissolved overnight in UltraPure™ DNase/RNase-free distilled water (Thermo Fisher Scientific, USA) overnight at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We eluted the RNA in 40 μl of DNase/RNase-free water and treated it with DNase I (Thermo Scientific, USA) following the manufacturer’s recommendations ...