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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Urotensin 2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Permeabilized embryos were incubated in blocking buffer (0.2% Triton X-100 in PBS + 2% BSA Fraction V 7.5% (Thermo Fisher Scientific, 15260-037) + 5% goat serum (Jackson Immunoresearch/Dianova ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA pol I and II visualization was accomplished by blocking (2) and incubating samples with rabbit anti-p-UBF1 (1:100 dilution; Invitrogen, PA5-105512) and mouse anti-RNA pol II (1:100 dilution ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated by a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The digested peptides were fractionated using a high-pH reversed phase (RP) peptide fractionation kit (Thermo Scientific). Fractions collected from the high-pH RP fractionation were further separated using on-line second-dimension low-pH reversed-phase chromatography via an Easy-nLC 1200 nanoflow-UHPLC system (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated using a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide concentration was quantified with a plate reader using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific). Finally ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 25 fmol/µl standard peptide mixture (Pierce™ Peptide Retention Time Calibration Mixture, ThermoFisher, 88321) and diluted to a final volume of 23 µl with water ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide concentration was measured using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific cat. # 23275). The digested peptides were subjected to simple C18 clean up using a C18 disk plug (3M Empore C18 ...
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bioRxiv - Neuroscience 2023Quote: ... desalted peptides was separated into 9 fractions with the high pH reversed-phase peptide fractionation kit (ThermoFisher). Fractions with increasing percentages of acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptide concentration in the supernatant was quantified using a quantitative colorimetric peptide assay from Thermo Fisher Scientific (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was recovered for peptide desalting using Pierce™ Peptide Desalting Spin Column (ThermoFisher: 89851) as described by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% TFA and peptide content was estimated with a Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The concentration of the peptides was determined using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Cat#23275). Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Cell Biology 2020Quote: ... and 1X PBS) before blocking for one hour with blocking buffer (Bovine Serum Albumin (Fisher Scientific, Fair Lawn, NJ), Normal Donkey Serum (Jackson ImmunoResearch Inc. ...
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bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... An aliquot of each peptide preparation was injected onto a PepMap RSLC C18 analytical column (2 µm, 100 Å, 75 µm x 25 cm, Thermo Scientific) and subject to a 90 min ...
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bioRxiv - Biochemistry 2019Quote: ... The peptides were loaded on a reversed-phase trap column (75 μm × 2 cm, 3 μm, C18, 100 Å, Thermo Scientific, Germany), and then the eluent was transferred to a reversed-phase analytical column (50 μm × 150 mm ...
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bioRxiv - Systems Biology 2021Quote: ... Peptide sample was loaded onto the pre-analytical column (100 µm x 2 cm, nanoViper C18, 5 µm, 100A; Thermo Fisher Scientific) at a flow rate of 5 µl/min ...
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bioRxiv - Molecular Biology 2022Quote: ... The peptides were separated on a 75 μm × 15 cm RP-HPLC analytical column packed with 2 μM C18 particles (Thermo Fisher Scientific) using an increasing gradient of 0.1% FA in ACN (8-35% over 100 min ...
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bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were separated by a PepMapTM RSLC C18 EasySpray column (2 µm, 100Å, 75 µm x 50 cm) (Thermo Fisher Scientific, UK), operating at 50°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm × 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were separated on an EASY Spray C18 column (50 cm x 75 cm, 2 µm particle size) (ThermoFisher Scientific, Toronto) using an EASY nLC-1200 ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were separated on an EASY-Spray PepMap RSLC C18 column (2 µm particle size, 75 µm × 50 cm; Thermo Scientific, ES801) heated to 55 °C using a flow rate of 400 nL/min ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were separated on an Acclaim PepMap C18 column (id 75 μm, length 50 cm, particle size 2 μm, Thermo Fisher Scientific) using a 10%-45% gradient of solvent B (80% acetonitrile ...
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bioRxiv - Microbiology 2023Quote: ... the valve switched to allow peptides to be separated on an Acclaim PepMap C18 nano column (2 μm, 75 μm x 25 cm, Thermo Fisher Scientific) at 35 °C in either 60-minute or 90-minute gradients of 5% to 35% buffer B (98% ACN with 0.1% formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... the valve switched to allow peptides to be separated on an Acclaim PepMap C18 nano column (2 µm, 75 µm x 25 cm, Thermo Fisher Scientific) at 35 °C in a 90 min gradient of 5% to 40% buffer B (98% ACN with 0.1% formic acid ...
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bioRxiv - Systems Biology 2023Quote: ... Peptides were separated on an analytical column (Easy-Spray™ PepMap™ RSLC C18, 2 μm, 75 μm × 50 cm, Thermo Scientific) at a voltage of about 2.4 kV and at a flow rate of 0.3 μL/min with mobile phases of 0.1% FA in water and in 95% ACN using a linear gradient ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were loaded onto an Acclaim PepMap 100 trap column (75 μm × 2 cm nanoViper, C18, 3 μm 100 Å, ThermoFisher Scientific) at flow rate of 2 μL/min ...
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bioRxiv - Microbiology 2023Quote: ... 4 µl of peptide mixtures were loaded onto an Acclaim PepMap precolumn (75 µm × 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm × 50 cm ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 µg of peptide sample was analyzed with a RSLCnano UHPLC system coupled to a Q-Exactive mass spectrometer (Thermo Fisher Scientific) using an acetonitrile gradient (3.2% - 60% ...
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bioRxiv - Cancer Biology 2019Quote: ... the sections were incubated overnight at room temperature with the indicated primary antibody prepared in blocking/permeabilization solution: mouse monoclonal antibody against HK-2 (1:100; Thermo Fisher, MA5-15679) or mouse monoclonal antibody against GLUT-3 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... a secondary antibody was applied to the sections for 2 hours (AlexaFluor goat anti-mouse 594 diluted 1:400 in blocking buffer; Life Technologies, Carlsbad, CA). Sections were again washed in PBS over 30 minutes prior to the application of mounting media and coverslips.
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bioRxiv - Cell Biology 2022Quote: ... Then samples were incubated for 30 min in blocking buffer containing 2% (w/v) BSA and then incubated with Alexa Fluor 488 phalloidin (1:400 dilution, Thermo Fisher Scientific, A12379) overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were rinsed in 0.02M KPBS and incubated for 2 hr at room temperature in blocking buffer containing goat anti-rabbit Alexa-Fluor conjugated secondary antibodies (Life Technologies, Carlsbad, CA). Sections were coverslipped using ProLong antifade mounting medium (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at RT and then in the blocking buffer containing monoclonal anti-GFP mouse antibody (GF28R, 1:3000, Thermo Fisher, #MA5-15256) and biotin-conjugated anti-ALFA nanobody (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... incubated at room temperature for one hour with Alexa Fluor 647-conjugated donkey anti-mouse IgG diluted in blocking buffer (2 μg/ml, Molecular Probes, Cat #A31571) followed by washes in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were first incubated in blocking solution (2% bovine serum albumin [Sigma-Aldrich] and 0.3% triton X-100 [ThermoFisher] in 1X PBS) for 45 min at constant agitation and 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Microbiology 2024Quote: ... diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich], and 0.05% Tween 20 [Thermo Fisher Scientific]) was added and plates incubated for 2 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: The peptide concentration was determined by using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, IL) and 180 µg peptides from each sample were loaded onto MacroSpin column (NestGroup ...
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bioRxiv - Immunology 2019Quote: ... containing LCMV peptides (1:1000, Peptide 2.0 Inc.) and Protein Transport Inhibitor Cocktail (1:500, Thermo Fisher Scientific). Cells were incubated for 4 hours at 37°C and then surface antigens were stained as described above ...
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bioRxiv - Microbiology 2021Quote: ... Labeled peptides of all samples were mixed equally and desalted by peptide desalting spin columns (Thermo Fisher, 89,852). A C18 column (Waters BEH C18 ...
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bioRxiv - Plant Biology 2022Quote: LC-MS/MS analysis of peptides and phosphopeptides – Peptides mixtures were analyzed by nanoflow Easy-nLC (Thermo Scientific) and Orbitrap hybrid mass spectrometer (Q-exactive HF ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide concentrations were determined using a fluorimetric peptide assay kit following the manufacturer’s instructions (Thermo Scientific, Cat#23290).
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bioRxiv - Plant Biology 2019Quote: ... followed by peptide fractionation using the Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Scientific, Rockford, IL). Eight fractions of peptides from each sample were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS ...