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Citations for Thermo Fisher :
351 - 400 of 4833 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and Pierce peptide desalting spin columns (89852, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the media was changed and two days later the cells were collected and plated on gamma irradiated MEF feeders (Gibco, A34181) in human ES media (DMEM/F12 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The secondary antibodies goat anti-rabbit IgG H&L (HRP) (GE NA934,1:4000) and HRP-labeled goat anti-human IgG (gamma chain) cross-adsorbed secondary antibody (Invitrogen, 62-8420) (1:1000) ...
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bioRxiv - Bioengineering 2023Quote: ... using the Bio-Rad Protein Assay Dye Reagent Concentrate (5000006) with 2 mg/mL Pierce bovine gamma globulin standard ampules from Thermo Fisher Scientific (23212 ...
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bioRxiv - Immunology 2023Quote: ... Eight days after subcutaneous immunisation of C57BL/6 mice with 50 μg of 4-Hydroxy-3-nitrophenyl acetyl hapten conjugated chicken gamma globulin (NP-CGG) in Imject Alum Adjuvant (ThermoFisher Scientific), 4.3 µg MAR hypoxia detecting probe (Goryo Chemical ...
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bioRxiv - Microbiology 2023Quote: ... Cells were always normalized to their optical density at 750 nm using a Helios Gamma UV-Vis Spectrophotometer (Thermo Fisher Scientific). Experiments in day-night conditions were performed in a separate day-night chamber ...
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bioRxiv - Bioengineering 2024Quote: ... under the proliferative condition at 33°C with 10 units/mL mouse interferon gamma supplemented in fresh in RPMI 1640 basal media containing HEPES and GlutaMAX (Gibco, #72400120) with 10% FBS and 1% pen-strep ...
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bioRxiv - Biochemistry 2023Quote: Cells were seeded and allowed to recover for 24 hours prior to being treated with 10 ng/mL interferon gamma (Gibco PHC4031) or vehicle (40 mM Tris pH 7.4) ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Developmental Biology 2021Quote: Phospho-peptides were fractionated using alkaline reversed phase chromatography (Pierce™ High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) into 12 fractions using increasing concentrations of acetonitrile from 10%-80% in basic binding buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... All ten vials of samples were combined and approximately 100 μg peptide digests from this mix were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to instructions from manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were briefly centrifuged at 13,000 g and peptide-containing supernatant was used for further analysis Peptides were fractionated into eight fractions using High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher). Peptide fractions were dried in SpeedVac (Eppendorf ...
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bioRxiv - Bioengineering 2021Quote: ... then incubated with 50 µL of 1 µM biotinylated peptide in peptide binding buffer (1% sheared salmon sperm DNA (Invitrogen, AM9680), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... and remaining ~1.1 mg of multiplexed peptides were used for phospho-peptide enrichment by High-Select Fe-NTA Phosphopeptide enrichment kit (Thermo Fisher) after manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The molecular mass of purified peptide was determined by 18% SDS-PAGE and the peptide concentration was obtained by Bicinchoninic protein assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: ... TMT-labeled peptides were pre-fractionated with the basic pH reversed-phase C18 peptide fractionation kit following manufacturer’s manual (Thermo Fisher, 84868). Typically ...
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bioRxiv - Biochemistry 2019Quote: ... peptide concentration and primary amine reactivity were quantified using the Pierce quantitative fluorometric peptide assay (Thermo Fisher Scientific; cat. no. 23290) and Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... were raised in rabbits by immunization with keyhole limpet hemocyanin-conjugated peptides and affinity-purified by columns conjugated with the peptides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The phosphopeptide enrichment step flow through and washes were dried down and approximately 120 µg peptides were fractionated using the TMT fractionation protocol of Pierce high pH basic reversed-phase peptide fractionation kit (Thermo Fisher Scientific™ Cat no 84858 ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 µL (90 µg) of reconstituted mixture of labeled peptides was fractionated using Pierce High pH Reversed-Phase Peptide Fractionation kit (84868, Thermo Fisher) according to the manufacturer’s protocol for TMT-labeled peptides ...
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bioRxiv - Biochemistry 2023Quote: The RC9 peptides with the sequence GGKGMGFGL was prepared using solid-state peptide synthesis with Fluorenylmethyloxycarbonyl (FMOC)-based chemistry by Life Technologies, Inc and purified by reversed-phase high pressure liquid chromatography (HPLC ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 7.5) followed by elution in 100 µl TBS + 150 ng/µL competitor peptide (Pierce 3x DYKDDDDK Peptide, Thermo Fisher Scientific) at 37 °C for 30 min.
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bioRxiv - Cell Biology 2023Quote: ... Dried peptides were resuspended in DI water and then quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The deuterium exchange levels were determined by subtracting the centroid mass of undeuterated peptide from the centroid mass of deuterated peptide using HDExaminer (Version PD1.4, Thermo-Fisher Scientific, USA).
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bioRxiv - Synthetic Biology 2023Quote: The determination of serum TNF-α and IFN-γ concentration was performed using Mouse IFN gamma Uncoated ELISA and Mouse TNF alpha Uncoated ELISA (Invitrogen). The protocols recommended by the vendor were strictly adhered to.
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bioRxiv - Biophysics 2023Quote: ... Absorbance values over 600-900nm were measured using a Thermo Spectronic Helios Gamma UV-Vis Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). This was compared to analytical data from Aymard et al ...
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bioRxiv - Plant Biology 2019Quote: ... Phospho-peptides were enriched from the remaining sample (900 µg) using High-Select Fe-NTA Phospho-peptide Enrichment Kit (A32992, Thermo Fisher Scientific). The TMT labeled samples were analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2019Quote: ... Lymphocyte samples were prepared as 50 ng/µL total sample peptide concentration with Pierce Peptide Retention Time Calibration Mixture (PRTC, Thermo Fisher Scientific) spiked in at a final concentration of 2 fmol/µL and queued in blocked and randomized order with two technical replicates analyzed per sample ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were fractionated into a final eight fractions using a Pierce High pH reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868), according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... A portion of the peptides from the samples were pooled and fractionated using a Pierce High pH Reversed-Phase (HPRP) Peptide Fractionation Kit (Thermo Fisher Scientific) to generate a spectral library ...
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bioRxiv - Microbiology 2022Quote: Peptide concentrations were measured for fraction two of each sample using the Quantitative Fluorometric Peptide Assay kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... An aliquot of the tryptic digests was used to determine the concentration of the peptide mixtures by using Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through containing endogenous peptides was collected and peptide concentration was measured using a bicinchoninic acid (BCA) protein assay (Thermo Scientific Pierce) as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... The rat tail collagen I protein and GFOGER peptide peptide were subsequently blocked using methyl-PEG24-amine (MA-PEG24) (Thermo Fisher Scientific). Post functionalization ...
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bioRxiv - Neuroscience 2023Quote: ... The combined isobaric-labeled peptide solution was fractionated into eight fractions per manufacturer’s instructions using high pH reversed-phase peptide fractionation columns (ThermoFisher Scientific, Cat# PI84868). All eight peptide solutions were dried in a speed vac ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared by 9ul per sample and separated using the High pH Reversed-Phase Peptide Isolation Kit (Thermo Fisher Scientific) as directed ...
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bioRxiv - Neuroscience 2023Quote: ... The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific, cat# 23275).
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bioRxiv - Cell Biology 2023Quote: ... Those peptides were divided into several fractions using the Pierce High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... The combined flowthrough and washes were dried down in a speed vac to completion for subsequent bench-top fractionation of non-phospho peptides by alkaline reversed phase chromatography (PierceTM High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) with a modified elution scheme as described in81 and MS analysis.
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bioRxiv - Neuroscience 2023Quote: ... The TMT labeled peptide pools were separated into eight fractions with the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific), evaporated ...
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bioRxiv - Molecular Biology 2024Quote: ... Pooled TMT-labelled peptides were cleaned and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to manufacturer’s instruction for TMT experiments ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were incubated in blocking buffer (10% Goat Serum (Invitrogen) in PBS-Triton ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor 647 Phalloidin (Invitrogen; 1:200 in blocking buffer) was applied to sections before mounting ...
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bioRxiv - Evolutionary Biology 2022Quote: ... diluted 1:300 in blocking buffer (REF A11012, Invitrogen, USA). Embryos were washed in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... followed by blocking in PBS Superblock (ThermoFisher Scientific, Cat. #37515) containing 4% normal goat serum (Jackson Laboratories ...
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bioRxiv - Genomics 2021Quote: ... 200 μL SuperBlock™ (TBS.) Blocking Buffer – Blotting (Invitrogen,37537) was added to each well and incubated for 2 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... After blocking with casein in phosphate buffered saline (PBS) (ThermoFisher) for 1 h at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... Sections were blocked in SEABLOCK blocking buffer (Thermo Fisher Scientific) or in 5% bovine serum albumin (BSA ...