Labshake search
Citations for Thermo Fisher :
351 - 400 of 595 citations for Terlipressin Acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Acetate and lactate were measured using an ICS-1100 series ion chromatograph (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a polysulfonate ion-exclusion column (Metrosep A Supp 5) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The mobile phase consisted of buffer A: 5 mM ammonium acetate in water (LC/MS grade, Thermo Scientific) and buffer B ...
-
bioRxiv - Developmental Biology 2020Quote: ... were used with Tris-Acetate SDS Running Buffer (pH 8.24) and the HiMark Pre-Stained Protein Standard (Invitrogen, LC5699). Wet transfer on a PVDF membrane was done overnight at 4 mA ...
-
Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... The pellet was resuspended in cold tagmentation buffer (33 mM Tris-acetate (pH = 7.8) (BP-152, Thermo Fisher Scientific), 66 mM K-acetate (P5708 ...
-
bioRxiv - Genomics 2020Quote: ... The pellet was resuspended in cold tagmentation buffer (33 mM Tris-acetate (pH = 7.8) (BP-152, Thermo Fisher Scientific), 66 mM K-acetate (P5708 ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.25 M cell equivalents were run on each lane of a 7% NuPage Tris-Acetate gel (Invitrogen, Carlsbad, CA) and then transferred to an Immobilon-FL PVDF membrane (EMD Millipore ...
-
bioRxiv - Developmental Biology 2019Quote: ... chilled on ice for 5 min and electrophoresed in a 3 – 8% Tris-Acetate NuPAGE gel (Novex Life Technologies). Transfer of the proteins onto a nitrocellulose membrane was done in transfer buffer (5% methanol ...
-
bioRxiv - Physiology 2019Quote: ... 25 µg or 50 µg of ventricular mouse heart lysate samples were loaded on a 1.0 mm-thick 10% Tris-acetate gel for high molecular weight proteins (Invitrogen). Gels were run at 150 V for 1 hour and then subsequently transferred to nitrocellulose membranes using the Biorad Turbo Transfer System (Biorad ...
-
bioRxiv - Immunology 2021Quote: ... and proteins with a molecular weight above 100 kDa were separated on 3-8% Tris/Acetate gels (Life Technologies) in MOPS Buffer (Invitrogen) ...
-
bioRxiv - Neuroscience 2020Quote: ... Digestion products were resolved in 3-8% tris-acetate SDS-PAGE followed and visualized by Zinc staining (Life Technologies). Images acquisition (Chemi-Doc Camera ...
-
bioRxiv - Biochemistry 2020Quote: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 3-8% Tris-Acetate gels (Invitrogen, Carlsbad, CA), as described previously [27] ...
-
bioRxiv - Microbiology 2021Quote: ... A total of 20 μg of whole cell lysate were resolved on a 3-8% Tris-Acetate NuPAGE (Invitrogen) and analyzed by immunoblotting with anti-SPTBN1 ...
-
bioRxiv - Immunology 2020Quote: ... following SDS-PAGE in reducing and non-reducing conditions using NuPAGE™ 3-8% Tris-Acetate gels (Life Technologies). Purified proteins were dialyzed overnight against PBS using Slide-A-Lyzer® dialysis cassettes (10 kDa MW cut-off ...
-
bioRxiv - Molecular Biology 2021Quote: ... PolyA RNA was ethanol precipitated with 2.5 M Ammonium Acetate and 70% ethanol in a solution containing 50 μg/ml GlycoBlue Co-precipitant (AM9515, Invitrogen). RNA was resuspended in 10 μl and fragmented with 10x Fragmentation Buffer (AM8740 ...
-
bioRxiv - Cell Biology 2020Quote: ... and run in pre-chilled 1X MOPS Buffer (Thermo, NP0001) or NuPage Tris-Acetate SDS running buffer (Invitrogen LA0041). Samples were typically run at 100 Volts for 90 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... RNA was recovered by precipitation with isopropanol in presence of 0.3 M sodium acetate pH 5.5 and 20 μg Glycoblue (Thermo Scientific). Recovered RNA was dephosphorylated with T4 PNK (NEB ...
-
bioRxiv - Biophysics 2022Quote: ... all samples were immediately buffer exchanged into an nMS-compatible solution (150 mM ammonium acetate, pH 7.5, 0.01% Tween-20) with a 40 kDa MWCO (ThermoFisher Scientific). For nMS analysis ...
-
bioRxiv - Molecular Biology 2020Quote: Purified PDX complexes at 0.2-0.4 mg/mL concentrations (10 µL each in 1xTBS with 4 mM DTT) were buffer exchanged into 200 mM ammonium acetate (pH 6.7) using Zeba 75 µL microspin columns from Thermofisher. Proteins were then loaded into pulled glass capillaries (GlassTip™ ...
-
bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA libraries in the flowthrough were precipitated at −20 °C overnight in ethanol with 0.3 M sodium acetate and 1 µl linear acrylamide (AM9520, Ambion). Purified libraries were further quantified and inspected on a Tapestation (Agilent Technologies ...
-
bioRxiv - Immunology 2019Quote: ... while proteins above 200 kDa were blotted on a 3-8% Tris-Acetate gels in MOPS Buffer (Life Technologies). Proteins were separated at 200V for approximately one hour and transferred onto either PVDF or nitrocellulose membrane using the TransBlot Turbo Transfer (BioRad ...
-
bioRxiv - Molecular Biology 2020Quote: The total volume of extracted DNA was precipitated with 1/10th volume of 3M sodium acetate (Invitrogen, Carlsbad, California) and double volume of chilled molecular biology grade ethanol (Sigma-Aldrich ...
-
bioRxiv - Genetics 2020Quote: ... Samples (5 μL each) were loaded into wells of a NuPAGE Tris-acetate 3 – 8% polyacrylamide gel (ThermoFisher Scientific) and electrophoresed for 60 min at 15 volts/cm ...
-
bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
-
bioRxiv - Molecular Biology 2022Quote: ... 25% Acetonitrile in Water (Triethylammonium Acetate TEAA – 1.0M, Sigma-Aldrich, Acetonitrile – HPLC Grade, Fisher Chemical, EDTA – 0.5M, pH 8.0, Thermo Scientific). The column was equilibrated with 40% Buffer B ...
-
bioRxiv - Developmental Biology 2022Quote: ... Ultrathin sections were stained with uranyl acetate and lead citrate and analysed with a Tecnai Spirit (Thermo Fisher Scientific) operated at 120 kV.
-
bioRxiv - Molecular Biology 2023Quote: ... DNA libraries in the flowthrough were precipitated at −20°C for 18 hours in ethanol with 0.3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion). Purified libraries were further quantified and inspected on an Agilent 4200 TapeStation (Agilent Technologies ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... K18 tau and αSyn fibrils were diluted to 2.5-10 µM in 5 mM acetate buffer (Fisher Scientific, Switzerland) at pH 4.5 and coupled for 750 s at a flow rate of 5 µL/min on all surfaces except for ZD150D ...
-
bioRxiv - Cell Biology 2023Quote: ... 25% of the total immunoprecipitated material (per condition) was loaded onto a 3-8% Tris-Acetate gel (Thermo Fisher), fully resolved gels were transferred to nitrocellulose membranes ...
-
bioRxiv - Zoology 2023Quote: ... The remaining cirDNA was precipitated by ethanol-acetate and then the concentration was determined by Qubit Fluorometer (Invitrogen, USA).
-
bioRxiv - Cell Biology 2023Quote: ... the RNA extracted using the TRIzol method was further purified using the ammonium acetate precipitation method (Ambion, Catalog: AM9070G). The quality of RNA was assessed using Tapestation from Agilent Technologies ...
-
bioRxiv - Cell Biology 2023Quote: ... boiled at 95°C for 5 min and loaded into a NuPAGE 3-8% Tris-Acetate Gel (Thermo Scientific) along with HiMark™ Pre-stained Protein Standard (Thermo Scientific LC5699) ...
-
bioRxiv - Neuroscience 2023Quote: ... The cytosol and membrane fractions were separated on the NuPAGE Novex Tris-acetate 3–8% gel (Thermo Fisher Scientific) or the PAG mini SuperSep Ace Tris-glycine 5–20% gel (FUJIFILM Wako Pure Chemical) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Gels were run at 150 V for 2h in 1× NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific). Protein was transferred onto a PVDF membrane (Merck ...
-
bioRxiv - Biochemistry 2023Quote: ... sample was buffer exchanged into 100 mM ammonium acetate using ZebaTM spin column (7 kDa MWCO, Thermo Fisher Scientific). Sample was loaded into Au/Pd-coated borosilicate emitters (Thermo Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 95C and subjected to NuPage 3-8% Tris-Acetate gel (#EA0375PK2, Invitrogen). Separated proteins were transferred to cellulose membrane using a semi-dry transfer method and blocked in TBS-T with 5% dry milk containing 2% normal goat serum for 1hr ...
-
bioRxiv - Cell Biology 2023Quote: ... for 30 min at room temperature using 10 mM Sodium Acetate [pH 5.0] buffer containing 5μg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Thermofisher, 22980) as coupling agent ...
-
bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
-
bioRxiv - Cancer Biology 2023Quote: ... samples were run in a 3-8% Tris-acetate gel with 1X NuPAGE MES Running buffer (Life Technologies, Invitrogen). A wet transfer was done overnight at 30 V ...
-
bioRxiv - Neuroscience 2023Quote: ... Electroporation solutions were filtered with 0.45 µm and 0.20 µm filters (Nalgene Syringe Filters, Cellulose Acetate, 4 mm, Thermo Scientific) before loading into the glass pipettes ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting supernatants were passed through a 0.22-μm cellulose acetate filter (Thermo Fisher Scientific catalog number F2517-1) by centrifugation at 6,700 × g for 10 min at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... SDS-PAGE was carried out on either NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in Table S5.
-
bioRxiv - Molecular Biology 2024Quote: ... and either strepdavidin (10 μg ml-1 in 10 mM sodium acetate pH 5.0) or a monoclonal anti-V5 antibody (R960252, Invitrogen; 2 μg ml-1 in 10 mM sodium acetate pH 5.0 ...
-
bioRxiv - Neuroscience 2024Quote: ... Solutions were then successively filtered using 0.45 and 0.20 µm cellulose acetate syringe filters (Invitrogen, 171-0020 & 171-0045). CA1 PNs were then electroporated as previously described20 ...
-
bioRxiv - Microbiology 2020Quote: ... at which point the aqueous layer was removed and transferred to a new tube containing 100 μl of 5 M ammonium acetate (Ambion), 20 μl of 5 mg/ml glycogen (Ambion) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
-
bioRxiv - Cell Biology 2020Quote: ... IP and lysate samples were separated by SDS-PAGE on precast 3-8% tris-acetate and 4-12% bis-tris acrylamide gels (Invitrogen) followed by transfer to 0.2 μm nitrocellulose membranes (Amersham) ...
-
bioRxiv - Genetics 2020Quote: ... Samples were loaded on 4-12% NuPAGE Bis-Tris gels or 3-8% NuPAGE Tris acetate gels (Thermo Fisher Scientific) and transferred to the nitrocellulose membranes (GE Healthcare ...
-
bioRxiv - Genomics 2021Quote: ... and the amplified fragments were visualized by electrophoresis on agarose gels (2% for DBP1 and 1% for RBP2a) in 1x TAE buffer (40 mM Tris-acetate, 1 mM EDTA) with 5 μg/ mL ethidium bromide (Invitrogen) in a horizontal system (Bio-Rad ...