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Citations for Thermo Fisher :
351 - 400 of 5337 citations for TNF alpha TNFSF2 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: Primary antibodies were purchased as follows: rat anti-alpha tubulin (MA1-80017, Thermo Fisher Scientific), mouse anti-alpha tubulin (62204 ...
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bioRxiv - Bioengineering 2020Quote: ... and kept in growth media consisting of minimum essential alpha medium (αMEM; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: BMDMs were generated by culturing mouse bone marrow cells in alpha-MEM (Fisher Scientific, MT15012CV) containing 10% (vol/vol ...
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bioRxiv - Cancer Biology 2022Quote: ... and mouse anti-alpha smooth muscle actin (Thermo Fisher Scientific; 14-9760-82; 1:400). The secondary antibodies were horseradish peroxidase- conjugated goat anti-rabbit IgG antibodies (Nichirei ...
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bioRxiv - Bioengineering 2022Quote: ... Isolated rat bone marrow MSCs were cultured in alpha MEM (ThermoFisher Scientific, Burlington ON, Canada) with 10% fetal bovine serum (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... washed 2x with PBS and changed to serum free alpha-MEM (Invitrogen, Waltham, MA, USA) with 1% pencilin-streptomycin ...
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bioRxiv - Physiology 2023Quote: Balb/c mice were injected subcutaneously (s.c.) with 40IU of EPO (Recombinant HuEpo-alpha, Gibco) for four consecutive days ...
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bioRxiv - Neuroscience 2022Quote: ... MSCs were cultured in alpha-modified Eagle’s medium (α-MEM, Gibco Invitrogen, Carlsbad, CA, USA) supplemented with 1% L-Glutamine (Macopharma) ...
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bioRxiv - Neuroscience 2022Quote: ... MSCs were cultured in alpha-modified Eagle’s medium (α-MEM, Gibco Invitrogen, Carlsbad, CA, USA) supplemented with 1% L-Glutamine (Macopharma) ...
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bioRxiv - Plant Biology 2023Quote: ... 0.03% (w/v) L-glutamine (Alpha Caesar #A14201) 2% (w/v) sucrose (Fisher Scientific #10634932), and 100 μM acetosyringone ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated with alpha-bungarotoxin conjugated with Alexa594 (α-btx594, 4h; Thermo Fisher Scientific) to stain the acetylcholine receptors (AChRs) ...
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bioRxiv - Cancer Biology 2022Quote: ... with the addition of Super Bright 600 anti-CD49f (integrin alpha 6) antibody (Thermo Scientific, 63-0495-42 ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in Alpha Minimum Essential Medium (α-MEM; GIBCO BRL, Grand Island, NY, USA) supplemented with 15% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Bioengineering 2019Quote: ... The resulting VH-(G4S)3-VL ScFv fragment was further fused at the N-terminus of the murine TNF gene through a S4G-linker and the final construct VH-(G4S)3-VL-(S4G)3-TNF was then cloned into the mammalian expression vector pcDNA3.1 (+) vector (Invitrogen). A VH-(G4S)3-VL ScFv fragment specific for hen egg lysozyme (KSF)(34) ...
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bioRxiv - Microbiology 2020Quote: ... In particular IFN-γ and TNF-α were evaluated by Mouse Custom ProcartaPlex 9-plex (Invitrogen, Thermo Fisher Scientific) and normalized at 2500 ug/ml of quantified proteins in lung supernatants.
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bioRxiv - Microbiology 2020Quote: ... In particular IFN-γ and TNF-α were evaluated by Mouse Custom ProcartaPlex 9-plex (Invitrogen, Thermo Fisher Scientific) and normalized at 2500 ug/ml of quantified proteins in lung supernatants.
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... this is equivalent to total of 190,000 cells) in the morning and stimulated overnight with 10 ng/mL TNF (PHC3015, Invitrogen). The RBC control was prepared by culturing a 2% haematocrit suspension in RPMI medium overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Developmental Biology 2021Quote: ... chicken TNF-α (LITAF, assay ID number Gg03364359_m1) and the reference gene chicken GAPDH (assay ID number Gg03346982_m1) (Thermofisher Scientific). The PCR cycle parameters were an initial denaturing step at 95 °C for 10 min followed by 45 cycles of 95 °C for 15 s and 60 °C for 1 min ...
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bioRxiv - Bioengineering 2019Quote: ... mice received an injection of rat anti-mouse tumor necrosis factor (TNF) antibody (2.5 mg/kg intraperitoneal, clone MP6-XT22, Thermo Fisher) or a control isotype antibody (IgG1 kappa ...
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bioRxiv - Immunology 2019Quote: Cells were stimulated with 100ng/mL of TNC-TNF for 2h and RNA was extracted using GENEzol Reagent (Geneaid) and cleaned using PureLink RNA Mini Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in culture supernatant was quantified by commercial ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). The measurement of OD (Optical density ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α) were measured using a custom 8-plex panel mouse ProcartaPlex assay (ThermoFisher Scientific, catalog# PPX-08-MXGZGFX), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were then stimulated with 50-100 ng/mL tumor necrosis factor (TNF; Miltenyi) or 100 μg/mL of the Dectin-1 ligand Curdlan (Wako biochemical) in flatbottom plates (Nunc) at 37°C ...
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bioRxiv - Microbiology 2024Quote: TNF-α concentration in cell culture supernatants was determined using Ready-SET-Go! ELISA kits (ThermoFisher, Cat. 88-7324-88) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... The amount of TNF-α and IL-6 in serum was quantified using commercial mouse ELISA kits (Thermo Fisher Scientific). For ex vivo experiments using splenic myeloid cells ...
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bioRxiv - Biophysics 2022Quote: HeLa (ECACC 93021013) and MCF-7 (ECACC 86012803) cells were cultured in MEM Alpha medium (Gibco), with GlutaMax (no nucleosides) ...
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bioRxiv - Neuroscience 2019Quote: ... samples were incubated in Alexa594-alpha-bungarotoxin (Invitrogen, B-13423, 50μg/ml, 1:500 in PBS). Tissue was washed 3x in PBS and mounted on a slide with Vectashield mounting media ...
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bioRxiv - Immunology 2022Quote: ... The cells were then resuspended with the same volume of pre-warmed MEM-alpha (Gibco, 12561) + 10% HI-FBS (Gibco ...
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bioRxiv - Immunology 2021Quote: The cloning reactions were used to transform OneShot DH5 Alpha cells (Thermo Fisher Scientific, Cat#: 12297016) according to manufacturer’s directions and plated on agar plates containing ampicillin and grown overnight ...
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bioRxiv - Neuroscience 2021Quote: ... the inoculum was removed and cells were overlaid with semisolid medium constituted of alpha-MEM (Gibco) containing 1.25% carboxymethyl cellulose (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... The NK- 92 MI (ATCC CRL-2408) cell line was grown in Alpha-MEM medium (Gibco) with 2mM L-Glutamine ...
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bioRxiv - Molecular Biology 2022Quote: ... NHDF (WT and PML KO cells) were cultured in alpha-modified Minimum Essential Medium (Life Technologies) supplemented with 15% fetal calf serum and GlutaMAX (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Wild-type HeLa cells (ATCC) and stable HeLa cell lines were maintained in MEM-alpha (Gibco), supplemented with 10% v/v fetal bovine serum at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2023Quote: ... alpha-MEM supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin/streptomycin (p./s.) (Gibco). All cultures were maintained in a 37□ incubator with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... The osteochondral explants were cultured overnight in alpha-Minimum Essential Medium (α-MEM; Gibco, Massachusetts, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Denuded oocytes were maintained at the GV stage in alpha-minimal essential medium (α-MEM) (Gibco) supplemented with 0.2 mM sodium pyruvate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... fixed oocytes were first incubated with a mouse monoclonal against alpha-tubulin (LifeTechnologies-Invitrogen, 13-800), followed by an incubation with chicken anti-mouse IgG conjugated to Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Immunology 2021Quote: ... 17-7311-81) and TNF-α (eBioscience, 12-7321-81)) were stained before fixation/permeabi-lization (Intracellular Fixation & Permeabilization Buffer Set, ThermoFisher).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... IL-6 and TNF-α in RAW264.7 cells culture supernatants were analyzed using ELISA kits (No. EMIL6RA, 88-7324-22, bms6002; Thermo Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... ELISAs were conducted following the manufacturers’ protocols, with the IL-1b and IFN-y (ELISAKIT.com, EK-0033 and EK-0002, respectively) and TNF-a (ThermoFisher Scientific KMC4022). Samples were diluted 1 in 5 and 1 in 10 for IL-1b ...