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Citations for Thermo Fisher :
351 - 400 of 7730 citations for Sulfo NHS LC Biotin sodium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with ice-cold PBS containing 1 mM CaCl2 and 0.5 mM MgCl2 and incubated with 1 mg/ml Sulfo-NHS-SS- biotin (Thermo Fisher Scientific, PI21331) for 15 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... using a Superdex S75 10/300 column in 1XPBS and chemically biotinylated using EZ-Link™ Sulfo-NHS-Biotin (Thermo Fisher Scientific) following manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: RPE1-hTERT cells were washed generously with ice-cold PBS prior to labelling with cell-impermeable 0.2 mg/mL Sulfo-NHS-SS Biotin (ThermoFisher Scientific, no. 21217) in PBS (pH 7.4) ...
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bioRxiv - Physiology 2024Quote: OK cells cultured on permeable supports under static or FSS conditions were washed with cold PBS and the apical surface biotinylated with 1 mg/mL EZ-Link Sulfo-NHS-SS-biotin (Thermo Scientific, 21331) in 0.5 mL TEA-buffered saline (TBS ...
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bioRxiv - Neuroscience 2024Quote: Cerebellar acute slices were washed in 37°C pre-warmed ACSF (see above) for 30 min before incubation in ACSF containing 1 mg/ml EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Fisher Sci) for 1h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... incubated with EZ-link NHS-biotin (Thermofisher) 2.5mM for 30 minutes on ice ...
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bioRxiv - Genetics 2020Quote: EZ-Link NHS-Biotin (ThermoFisher Scientific 20217);
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bioRxiv - Cancer Biology 2022Quote: ... The SKBR3 cell-membrane proteins were isolated by either the biotinylation of amino (EZ-Link Sulfo-NHS-SS-Biotin, Thermo Fisher Scientific, Rockford, IL) or oxidized glycan (EZ-Link Alkoxamine-PEG4-Biotin ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed once with PBS containing both calcium and magnesium and labeled with 0.5 mg of membrane-impermeable EZ-linkTM Sulfo-NHS-Biotin (Thermo Fisher Scientific, Rockford, IL, USA) per plate for 30 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: A biotin tracer (EZ-Link™ NHS-Biotin; Thermo Fisher Scientific, Australia) was used to test the patency of the blood-testis barrier in Axdnd1-/- mice as described previously (44) ...
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bioRxiv - Biochemistry 2021Quote: ... S1 subunit and the S protein (InVivo Biotech) were biotinylated using 10 molar excess of NHS-LC-Biotin (Thermo Scientific). Immunoassay plates were coated with 2.5 μg/ml recombinant angiotensin converting enzyme-2 (ACE2 ...
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bioRxiv - Immunology 2021Quote: ... recombinant HAΔTM H1N1 A/Michigan/45/2015 and HAΔTM H3N2 A/Hong Kong/4801/2014 were biotinylated with a 20-fold excess of 10 mM EZ-Link NHS-LC-biotin (ThermoFisher) and unbound biotin was removed using a Zeba Spin Desalting Column (ThermoFisher) ...
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bioRxiv - Biophysics 2023Quote: ... Claudins were labeled with NHS-PEG4-Biotin (ThermoFisher) using 4:1 biotin:claudin molar excess overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific) was dissolved in DMSO and added to the concentrated nanobody at a molar ratio of 10:1 ...
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bioRxiv - Microbiology 2019Quote: ... and monoclonal mouse IgG α-PAN-pLDH (PA-2, AccessBio, Somerset, NJ) were biotinylated using the EZ-Link Sulfo-NHS-Biotin Kit (21435, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions with minor modifications (See Additional file 1 ...
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bioRxiv - Immunology 2024Quote: ... and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA ...
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bioRxiv - Cell Biology 2023Quote: ... The carboxyl groups on the bead surfaces were functionalized with amine-reactive groups via N- ethyl-N’-(3-(dimethylamino)propyl)carbodiimide (EDC) and sulfo-N-hydroxysuccinimide (sulfo-NHS, Thermo Fisher) crosslinking for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and 5 mg/mL Sulfo-NHS (N-hydroxysulfosuccinimide; ThermoFisher Scientific; Waltham, MA, USA). Activated microspheres were washed twice in 0.05 M MES (2-(N-morpholino)ethanesulfonic acid ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated using the EZ-link Sulfo-NHS biotinylation kit (Thermo Scientific, 2143) according to the protocol provided ...
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bioRxiv - Immunology 2023Quote: ... was biotinylated with 80-fold molar excess of NHS-PEG4-Biotin solution from the EZ-Link™ NHS-PEG4-Biotin kit (ThermoFisher, #A39259). Excess biotin was removed by buffer exchange of protein into sterile 1X PBS using Zeba™ Spin Desalting Columns ...
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bioRxiv - Immunology 2020Quote: ... and soluble cytosolic protein (SCP) fractions were extracted from Chinese hamster ovary (CHO) cells and biotinylated using NHS-LC-Biotin (Thermo Fisher Scientific) reagent ...
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bioRxiv - Biophysics 2022Quote: ... Labeled tubulin was prepared by incubating polymerized microtubules with tetramethylrhodamine succinimidyl ester (C-1171; Thermo Fischer) or NHS-LC-biotin (21336; Thermo Fisher Scientific) for 30 min at 37°C (32) ...
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bioRxiv - Cell Biology 2019Quote: ... Labeling efficiency with biotin-PEG4-NHS (Thermo Scientific, A39259) was not calculated.
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bioRxiv - Immunology 2020Quote: ... was biotinylated using NHS-PEG4-Biotin (Thermo Fisher, 21330). The protein was de-salted using Zeba Quick Spin columns (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeling efficiency with biotin-PEG4-NHS (Thermo Scientific: A39259) was not calculated.
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bioRxiv - Immunology 2024Quote: ... Biotinylated using EZ-Link NHS Biotin (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: Fluorescence signal was measured from biotinylated peptides (biotin-NHS or biotin-SS-NHS) using Quantitative Fluorometric Peptide Assay (Thermo Scientific Pierce, cat.no.: 23290) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... A freshly prepared 15mM stock of Sulfo-NHS-acetate in BCO (Thermo Fisher, # 26777) was added to a final concentration of 0.5mM ...
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bioRxiv - Immunology 2020Quote: ... and 5 mg/mL Sulfo-NHS (N-hydroxysulfosuccinimide; Thermo Fisher Scientific; Waltham, MA, USA). Activated microspheres were washed twice in 0.05 M MES (2-(N-morpholino ...
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bioRxiv - Microbiology 2021Quote: ... were carboxy-coupled to magnetic Luminex microplex carboxylated beads (Luminex Corporation) using NHS-ester linkages with Sulfo-NHS and EDC (Thermo Fisher) and then incubated with serum (IgG1 ...
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bioRxiv - Immunology 2021Quote: ... were carboxyl coupled to magnetic Luminex microplex carboxylated beads (Luminex Corporation) using NHS-ester linkages with Sulfo-NHS and EDC (Thermo Fisher), and then incubated with serum for 2 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Antigens of target were covalently linked to carboxyl group-labeled MagPlex microspheres (Luminex) through NHS-ester linkages using Sulfo-NHS and EDC (Thermo Fisher). Serum and BAL samples were diluted (serum isotypes/subclasses and FcγR binding ...
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bioRxiv - Immunology 2024Quote: ... Antigens of target were covalently linked to the carboxyl group-labeled MagPlex microspheres (Luminex) through NHS-ester linkages using Sulfo-NHS and EDC (Thermo Fisher) as described for Luminex ...
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bioRxiv - Biochemistry 2021Quote: ... Detection antibodies were conjugated to biotin using EZ-Link NHS-PEG4 Biotin (Thermo Fisher Scientific). The following antibodies were used as capture antibodies for tetraspanins ...
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bioRxiv - Biochemistry 2021Quote: ... All targets were chemically biotinylated using NHS-Biotin (ThermoFisher #20217) according to the manufacturer protocol ...
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bioRxiv - Biophysics 2019Quote: ... or EZ-Link™ NHS-Biotin (Thermo Scientific, Cat# 20217) at amino-C6-dT (amine modification with amino-modifier C6-T ...
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bioRxiv - Biophysics 2021Quote: ... was biotinylated using biotin-NHS (Thermo Scientific, catalog number: 21343). Flow chambers for microscopy were assembled by sandwiching a piece of double-sided tape between a slide and a plasma cleaned coverslip (Fisherbrand ...
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bioRxiv - Biochemistry 2022Quote: ... incubated with 40 μM NHS-PEG4-Biotin (cat. #A39259, ThermoFisher) for 1h on ice and then resuspended with 2 mL of 3C Elution buffer (600 μg PreScission protease diluted in CSF-XB ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were biotinylated using NHS-PEG4-Biotin (Thermo Scientific, USA) following the manufacturer recommendations ...
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bioRxiv - Bioengineering 2023Quote: Uricase was biotinylated using EZ-link NHS-biotin (Thermo Scientific). Unreacted NHS-biotin was removed using Zeba spin desalting columns ...
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bioRxiv - Bioengineering 2024Quote: Rasburicase was biotinylated using EZ-link NHS-biotin (Thermo Scientific). Unreacted NHS-biotin was removed using Zeba spin desalting columns ...
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bioRxiv - Immunology 2021Quote: ... were covalently coupled to Luminex microplex carboxylated bead regions (Luminex Corporation) using NHS-ester linkages with Sulfo-NHS and EDC (Thermo Fisher Scientific) according to manufacturer recommendations ...
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bioRxiv - Immunology 2021Quote: ... were covalently coupled to Luminex microplex carboxylated bead regions (Luminex Corporation) using NHS-ester linkages with Sulfo-NHS and EDC (Thermo Fisher Scientific) according to manufacturer recommendations ...
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bioRxiv - Immunology 2020Quote: ... Purified antigens were biotinylated using an EZ-Link Sulfo-NHS-LCBiotinylation kit (Thermo Fisher Scientific) using a 1:1.3 M ratio of biotin to Ag ...
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bioRxiv - Biophysics 2023Quote: ... Excessive Sulfo-DBCO-NHS was then removed using 7K MWCO Zeba Spin Desalting columns (ThermoFisher). Azide modified DNA strands was added to DBCO modified anti-CD28 at a molar ratio of 12:1 ...
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bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was biotinylated using N-hydroxysuccinimide polyethylene glycol biotin (NHS-PEG4-biotin, ThermoFisher) by mixing 5.6 μM claudin-4 with 16.8 μM NHS-PEG4-biotin ...
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bioRxiv - Biophysics 2021Quote: ... Alexa Fluor 405 NHS ester (A30000) and EZ-Link NHS-PEG4-Biotin were purchased from Thermo Fisher. ATTO 647N NHS ester (18373-1MG-F) ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... using the EZ-Link Hydrazine-LC-Biotin kit (Thermofisher). Solid phase binding assays were performed essentially as described previously [47] ...
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bioRxiv - Neuroscience 2022Quote: Deeply anesthetized 8-9 week old male and female C57BL/6JOlaHsd mice were perfused for 5 min with sulfo-NHS-biotin (4 mg/20 g mouse body weight, Thermo Scientific, cat no. 21217, dissolved in 20 ml PBS). Brains were dissected and handled as described under tissue preparation section.
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bioRxiv - Microbiology 2022Quote: ... Unbound biotin or SULFO-TAG was removed using Zeba spin desalting columns (ThermoFisher Scientific), and the incorporation ratio for each label was measured (See Dataverse file) ...