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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Recombinant Monkey CCL19 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
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bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: African green monkey kidney cells (Vero; Nuvonis Technologies) were maintained in OptiPRO SFM (Life Tech) containing 2× GlutaMAX (Gibco). Human embryonic kidney cells (293T ...
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bioRxiv - Neuroscience 2020Quote: ... A third monkey was injected with a cholera toxin subunit B-Alexa Fluor 647 conjugate (CTB-AF647; C34778, Invitrogen) at the quadrigeminal cistern ...
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bioRxiv - Microbiology 2021Quote: The African vervet monkey kidney Vero (CCL-81) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM high glucose, Gibco) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: 293T (human kidney epithelial) and Vero-SLAM (African green monkey kidney) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2020Quote: ... MCF-10 human mammary epithelial cells and COS-7 fibroblast-like monkey cells were maintained in DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 2% penicillin/streptomycin.
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bioRxiv - Microbiology 2021Quote: The African green monkey Vero E6 cell line (ATCC CRL-1586) was maintained in Dulbecco’s minimal essential medium (DMEM) (Gibco), high glucose supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... human hepatoma Huh-7.5 (66) and African green monkey kidney Vero cells were all maintained in Dulbecco’s modified Eagle’s medium (DMEM; ThermoFisher, 11965118) supplemented with heat-inactivated 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Vero76 cells (African green monkey kidney, ATCC CRL-1587) were cultured in Dulbecco’s Modified Eagle Medium (Gibco, 41965-039) supplemented with 10% Fetal Bovine Serum (Gibco ...
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bioRxiv - Microbiology 2020Quote: Vero E6 cells (African green monkey kidney, ATCC) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Scientific, USA) containing 10% fetal bovine serum (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... African Green monkey kidney cells (Vero E6, ATCC) were grown in MEM+ Earl’s Salts + L-Glutamine (Gibco 11095-080). This media was supplemented with penicillin/streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... and Venus-Tau-Teal [17] were expressed in CV-1 African green monkey kidney fibroblasts using Lipofectamine 3000 (ThermoFisher). The cells were maintained in phenol red-free Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum to 10% by volume and gentamycin to 50 μg/ml.
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bioRxiv - Microbiology 2023Quote: ... Baby Hamster Kidney (BHK-21) cells and African green monkey kidney (Vero) cells (ATCC) were grown in Eagle’s minimal essential medium (MEM; Gibco) with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... African green monkey kidney epithelial cells (Vero cells, ATCC CCL-81) were maintained in minimal essential medium (MEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... The VeroE6 cell line (African green monkey kidney cells) was grown in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: Clarified serum from each animal was subjected to the Cytokine 29-Plex Monkey Panel (ThermoFisher Scientific, Waltham, MA, USA). Each sample was run in duplicate according to manufacturer instructions and average values were ascertained via the standard curve generated for each cytokine per run ...
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bioRxiv - Cell Biology 2024Quote: ... African green monkey kidney fibroblast-like cell line (COS-7) was maintained in minimal essential medium (MEM, Gibco/BRL) supplemented with 5% (v/v ...
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bioRxiv - Zoology 2024Quote: ... derived from Aedes albopictus and Vero cells (ATCC-CCL-81) derived from green monkey (Chlorocebus sabaeus) kidney were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, France) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2024Quote: ... Clarified serum from each animal was subjected to the Cytokine 29-Plex Monkey Panel (ThermoFisher Scientific, Waltham, MA, USA), measuring TGF-β ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).