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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... All liquid cultures were agitated at 250 rpm (E. coli) or 180 rpm (P. putida) in an orbital MaxQ™ 8000 shaker incubator (ThermoFisher Scientific, Waltham, MA, USA). Solid media contained 15 g L−1 agar ...
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bioRxiv - Cell Biology 2023Quote: ... Expression constructs were made by LR reaction using the following destination vectors: pDEST15 (for N-terminal GST-fusion in E. coli, cat#11802014, Thermo Fisher Scientific, Waltham, MA, USA) and pcDNA-DEST53 (for N-terminal GFP fusion in mammalian cells ...
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bioRxiv - Microbiology 2020Quote: ... All proteins were expressed in Escherichia coli one-shot BL21star (DE3) cells (Invitrogen, Carlsbad, CA, USA), as previously described [13] ...
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bioRxiv - Biophysics 2022Quote: ... The protein expression was done in Escherichia coli BL21(DE3) pLysS cells (Invitrogen, Grand Island, NY). Samples for MAS ssNMR were uniformly 13C ...
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bioRxiv - Bioengineering 2021Quote: ... The positive control consisted of 100 – 500 ng recombinant Protein A (ThermoFisher Scientific, Santa Clara, CA). The negative control consisted of ~2 μg wt-TMV from purified N ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell supernatant containing recombinant mAb was passed over Protein A Agarose resin (Thermo Fisher Scientific). Protein A resin was extensively washed with 25 mM Phosphate pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant Cripto protein or transfected with Cripto expression plasmid (pcCripto) using Lipofectamine2000 (Life Technologies, Carlsbad, USA) according to the manufacturer`s instructions ...
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bioRxiv - Pathology 2022Quote: TRP120 and TRX recombinant proteins were desalted using Zeba spin desalting columns (Thermo Fisher Scientific, MA) as indicated by the manufacturer protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 µL of the cleared lysates were added with 20 µL Recombinant Protein G Agarose (Invitrogen) and rotated for 30 min at 4 °C to preclear ...
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bioRxiv - Microbiology 2021Quote: ... we conjugated recombinant RBD proteins with Alexa Fluor 488 or Alexa Fluor 594 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Quantification of the recombinant proteins was performed by Pierce™ BCA Assay (Thermo Fisher Scientific 23225) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Microbiology 2022Quote: ... we conjugated recombinant RBD proteins with Alexa Fluor 488 or Alexa Fluor 647 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The Pbc43opt recombinant protein was extracted from inclusion bodies using the Inclusion Body Solubilization Reagent (ThermoFisher). The solubilized protein was also purified using TALON® metal affinity resin ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... cell lysate supernatants were mixed with antibodies and recombinant protein G-Sepharose 4B beads (Thermo Fisher) at 4°C for overnight ...
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bioRxiv - Plant Biology 2020Quote: ... Serum was used for antibody affinity purification to the STS1 recombinant protein using magnetic beads (Invitrogen). Validation of antibody was carried out by immunoblot with total protein extract from sts1 complemented lines and sts1 mutant as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... All recombinant proteins were examined by SDS-PAGE along with Pierce BSA mass standards (Thermo Fisher), and their concentrations were calculated from infrared scanning of Coomassie-stained gels (Odyssey Fc Imaging System ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guideline 66 ...
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bioRxiv - Neuroscience 2022Quote: ... and the recombinant proteins were purified using HisPur Cobalt Superflow Agarose (Thermo Scientific, Waltham, MA, USA), dialyzed against 1× phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant tau was labeled using an Alexa Fluor 647 Protein Labeling Kit (Cat No. A20173, Invitrogen) with 500 mg of recombinant protein input at a concentration of 1 mg/mL according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Recombinant Omicron RBD proteins from variants BA.2 and BA.4.5 were obtained from Fisher Scientific. To determine the specificity of mAbs ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 150 ng/µl sgRNA and 500 ng/ul recombinant TrueCut Cas9 protein (Thermo Fisher, A36496) using Invitrogen Neon Electroporator with 2 pulses of 1200 V ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant Bd37 and Venus (control) proteins were purified by using ProBond Purification system (Thermo Fisher Scientific) according to the manufacture’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... recombinant Cas9 protein (100 ng/μl; GeneArt Platinum Cas9 Nuclease, Thermo Fisher Scientific, Waltham, MA, USA), and ssODNs (400 ng/μl ...
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin was visualized with rat anti-E-cadherin (Invitrogen #13-1900), both at 1:100 concentrations as per manufacturer protocol ...
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin (Invitrogen ECCD2) and GAPDH (Thermo Fisher MA5-15738 ...
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bioRxiv - Developmental Biology 2019Quote: ... 2% (E-Gel, GIBCO). DNA fragments from the complete and size-selected library were detected and confirmed ...
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bioRxiv - Physiology 2023Quote: ... E (Life Technologies, USA). Each resulting barcoded library was quantified and assessed for quality using the Agilent Bioanalyzer ...
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bioRxiv - Developmental Biology 2024Quote: ... E-CAD (Invitrogen, 14324980), FOXP2 (1:250 ...
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bioRxiv - Biophysics 2020Quote: ... coli (GeneArt, ThermoFisher) and inserted in pET21a(+ ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli TOP10 (Invitrogen). For wild-type TOPO-blaOXA-48 ...
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bioRxiv - Microbiology 2021Quote: ... coli (Invitrogen C404003). Individual colonies were miniprepped and sequenced.
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bioRxiv - Molecular Biology 2020Quote: ... coli (Thermo Fisher). These plasmids were used in the Bac-to-bac expression system ...
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bioRxiv - Molecular Biology 2021Quote: ... coli cells (ThermoFisher) were transformed with the ligation reactions and single colonies selected using 34 μg/ml chloramphenicol on LB-agar ...
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bioRxiv - Genomics 2020Quote: ... coli RNaseH (Invitrogen)] ...
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bioRxiv - Immunology 2020Quote: ... coli cells (Invitrogen) were transformed with the plasmid ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli Pir1+ (Invitrogen). eSLC strains were grown in LB media with 50 μg/mL kanamycin (pSC01 ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli TOP10 (Invitrogen) was used for cloning and competent cells were prepared in-house (106 – 107 cfu/μg) ...
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bioRxiv - Genetics 2021Quote: ... coli (Invitrogen LS18263012) were transformed with 5 μL of reaction.
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bioRxiv - Genetics 2020Quote: ... coli cells (ThermoFisher), verified by sequencing and purified with a Plasmid Midi kit (Qiagen).
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bioRxiv - Neuroscience 2020Quote: ... coli (Invitrogen, P35361) bioparticles were diluted to 0.25mg/ml and pHrodo-labelled dextran (10,000MW ...
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bioRxiv - Cell Biology 2020Quote: ... coli cells (Invitrogen), along with the tetracycline-inducible ...
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bioRxiv - Neuroscience 2020Quote: ... coli (ThermoFisher; P35366) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (ThermoFisher), purified ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (ThermoFisher), purified ...
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bioRxiv - Bioengineering 2020Quote: ... coli DH5α (Invitrogen) was used for routine gene cloning works ...
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bioRxiv - Bioengineering 2020Quote: ... coli DH5α (Invitrogen) was used as a host strain for routine gene cloning ...