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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Human foreskin fibroblasts (HFFs) monolayers were grown in DMEM GlutaMAXTM (4.5 g/L D-glucose) (Life Technologies #10-566-024) supplemented with 2 mM L-glutamine ...
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bioRxiv - Genetics 2022Quote: Cells were lysed and proteins were extracted from fresh fibroblast cells with RIPA buffer (Thermo Scientific) supplemented with protease and phosphatase inhibitor mini-tablet (Pierce ...
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bioRxiv - Genetics 2020Quote: ... Primary neonatal Human Umbilical Vein Endothelial Cells (HUVEC) were sourced from three suppliers to provide genetic diversity (Lonza, Gibco, PromoCell). HUVECs were expanded in endothelial cell growth medium (Sigma ...
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bioRxiv - Immunology 2021Quote: ... as a Cas9-RNP complex (1:1 Cas9:gRNA molar ratio) were delivered primary human CD3+ T cells after 2 days of Anti-CD3/-CD28 Dynabead (ThermoFisher) stimulation ...
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... T47D (ATCC HTB-133) or primary cells from human tumors were fixed by adding 8% PFA (Thermo Scientific cat#28908) directly to the samples (1:1 v/v with media ...
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bioRxiv - Cancer Biology 2023Quote: ... Viably frozen primary AML cells were thawed and CD3-depleted with biotinylated anti-human CD3 (SK7) and biotin-binder Dynabeads (Invitrogen) and subsequently injected via the lateral tail vein into 2.8 Gy irradiated (24h before transplant ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... and fibroblast cell transfections were performed by using Lipofectamine 3000 (cat#: L3000015, Invitrogen), and si-RNA knockdown experiments were performed using Lipofectamine RNAiMAX reagent (cat# ...
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bioRxiv - Cell Biology 2020Quote: RNA was obtained from fibroblast cells using Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were seeded in 2mL of mouse embryonic fibroblast (MEF) medium [DMEM (Invitrogen) supplemented with 10% FBS (Omega) ...
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bioRxiv - Cell Biology 2019Quote: ... All fibroblasts were grown in T25 or T75 cell culture flasks (ThermoFisher Scientific) with Minimum Essential Medium Eagle supplemented with 10% fetal bovine serum ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were cultured on irradiated CF1 Mouse Embryonic Fibroblasts (Thermo Fisher Scientific 15943412) in KOSR-based medium containing 80% DMEM/F12 (Thermo Fisher Scientific 31330095) ...
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bioRxiv - Developmental Biology 2022Quote: ... ESCs were routinely maintained on an in-house generated mitotically inactive primary mouse embryo fibroblast feeder layer in ES medium (81.8% Knockout DMEM (Gibco, 10829-018); 15% gamma irradiated fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Human dermal primary fibroblasts isolated from a PD patient (ND39528) and a healthy control (ND36091) were cultured in DMEM (11995-065, Thermo Fisher) supplemented with 10% fetal bovine serum (900-108 ...
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bioRxiv - Microbiology 2019Quote: Human primary B cells from adenoids were separated from T cells by rosetting with sheep erythrocytes (purchased from Thermo Scientific Oxoid, cat. no. SR0051D) and purified by Ficoll density gradient centrifugation as described in the previous paragraph ...
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bioRxiv - Bioengineering 2024Quote: Engineered cardiac tissues and hiPSC-cardiomyocytes cultured with patient IgG were fixed and incubated with secondary anti-human IgG (Thermo Fisher Scientific, cat. no. A-11013) to visualize cellular autoantibody targets ...
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bioRxiv - Neuroscience 2022Quote: BWCFF-S ACTB Thr120Ile patient-derived fibroblasts were thawed in fibroblast medium I (DMEM (Gibco), supplemented with 10% FCS (PAA) ...
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bioRxiv - Cell Biology 2019Quote: ... cardiac Troponin T (cTnT, 1:100; Thermo Scientific, MS-295-P1), Wheat Germ Agglutinin (WGA ...
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bioRxiv - Developmental Biology 2022Quote: ... cardiac progenitors were then re-plated in RPMI/B-27 (Gibco) with 100 μg/mL Vitamin C (Vit ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Flour 488 for cardiac sections of hypertrophic mice (Life Technologies) or CF405 for all other in vitro cultures (Biotium) ...
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bioRxiv - Physiology 2020Quote: Total cardiac RNA was extracted by TriReagent (Thermo Scientific, Vilnius, Lithuania) and the quality and quantity of RNA were determined by agarose gel electrophoresis and NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-cardiac Troponin T (Thermo Scientific, MS-295-P, 1:100), or TUBB3 (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-cardiac troponin T (1:500; ThermoFisher MA5-12960), mouse monoclonal anti-vinculin (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... both human primary myogenic progenitors isolated from female donors (n=2-5 per experiment) and commercialised human primary skeletal muscle progenitors (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... gondii tachyzoites were cultured in human foreskin fibroblasts (HFF) and in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAX (Invitrogen, California, United States) supplemented with 10% fetal bovine serum and maintained at 37°C with 5% CO2.
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in a confluent monolayer of human foreskin fibroblasts (HFFs) maintained in Dulbecco’s Modified Eagle medium GlutaMAX™(DMEM+ GlutaMAX™, Gibco) supplemented with 10% foetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2021Quote: DiPS 1016 SevA hiPSC line derived from human skin fibroblasts were seeded and kept in culture on Geltrex (1% Invitrogen, USA)-coated culture flasks using mTeSR (StemCell Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and ME49 hxgprt-ko ku80-ko (ME49) were grown in confluent human foreskin fibroblasts (HFF, ATCC SCRC-1041) in DMEM (Gibco 41965039) supplemented with 5% fetal calf serum and 40 μg/ml gentamycin (Gibco 15710-049) ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Parasites were maintained in monolayer cultures of human foreskin fibroblasts (HFFs, ATCC® SCRC-1041) in Dulbecco’s Modified Eagles Medium (DMEM, GIBCO, USA) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: The microdevices containing normal human lung fibroblasts with or without macrophages were fixed using a solution of 2% glutaraldehyde (ACROS organics) in PBS (containing Ca2+/Mg2+ ...
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bioRxiv - Neuroscience 2022Quote: ... Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... gondii tachyzoites were cultured within human foreskin fibroblasts (HFFs) at 37°C under 5% CO2 in D10 medium consisting of DMEM (Fisher Scientific) supplemented with 10% heat-inactivated bovine calf serum (Cytiva) ...
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bioRxiv - Biochemistry 2020Quote: Mouse fibroblast cells and hTERT transformed RPE1 cells were cultured in DMEM and Ham’s F12/DMEM (Invitrogen), respectively ...
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bioRxiv - Bioengineering 2023Quote: NIH3T3 fibroblast cell lines and HaCaT keratinocyte cell lines were cultured using DMEM high glucose media (Gibco) containing 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Immunology 2022Quote: ... Primary B cells were plated at 2-3×106 cells/ml in primary B cell medium (DMEM (Gibco) containing 10% FBS (Sigma) ...
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bioRxiv - Bioengineering 2019Quote: ... Primary MMP13 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-14247) was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human ...
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bioRxiv - Biochemistry 2020Quote: ... coated with primary antibody CD63 (Exosome-Human CD63, Thermo Fisher - Scientific - Invitrogen™) and CD81 (Exosome-Human CD81 ...
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bioRxiv - Biochemistry 2020Quote: ... coated with primary antibody CD63 (Exosome-Human CD63, Thermo Fisher - Scientific - Invitrogen™) and CD81 (Exosome-Human CD81 ...
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bioRxiv - Molecular Biology 2023Quote: The RNA was extracted from human primary OCs using the TRIzol Reagent (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 600 ng total RNA of human primary mesenchymal stem cells were reversely transcribed using the RevertAid RT Reverse Transcription Kit (K1691, Thermo Scientific) according to the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell death analysis was performed by staining primary human hepatocytes with Dead Cell Apoptosis Kit with Annexin V FITC and PI (V13242, Thermo Fisher). PI+ve cells were then quantified with the flow cytometer (Fortessa ...
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bioRxiv - Cancer Biology 2021Quote: ... tissue culture flasks until they reached 70-80% confluence in human ectocervical primary cell medium (Advanced DMEM/F-12 (Invitrogen, 12634) supplemented with 12 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... primary T cells were activated and stimulated with a 1:1 ratio of anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher) to cells in X-vivo 15 medium supplemented with 5% human AB serum (Heat inactivated) ...
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bioRxiv - Cell Biology 2020Quote: ... primed PSCs were maintained on MMC-treated primary mouse embryonic fibroblasts (PMEFs) in DFK20 media consisting of DMEM/F12 (Thermo Fisher Scientific), 20% Knockout Serum Replacement (KSR ...
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bioRxiv - Neuroscience 2024Quote: iPSCs from a healthy donor were already available and obtained upon reprogramming of primary fibroblasts with CytoTune®-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific) after receiving written informed consent (Ethic committee approval n ...