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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The cells were then washed with PBS five times for 10 mins per wash and stained with biotin-XX goat anti-mouse IgG antibody (Invitrogen, B2763). The cells were then washed with PBS five times for 10 mins per wash and stained with Qdot™ 525 (QD525 ...
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bioRxiv - Biophysics 2022Quote: ... The cells were then washed with PBS five times for 10 mins per wash and stained with Biotin-XX goat anti-mouse IgG antibody (Invitrogen, B2763) at room temperature in PBS with 1:200 for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... incubated for 5 min with 25 pM (single-label) or 5 pM (dual-label) biotin-tagged α-FLAG monoclonal antibody (ThermoFisher Scientific #MA1-91878-BTIN ...
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bioRxiv - Neuroscience 2023Quote: ... Some tissues were incubated with a mouse biotin-conjugated monoclonal antibody against HuC/HuD (1:200, Fisher Scientific, East Greenwich, RI) for 48 h at 4 °C ...
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bioRxiv - Biophysics 2023Quote: We performed the biotinylation of single chain antibodies and of Gal-9 with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher). For this ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were first stained for COL1A1 than biotin-conjugated SCA1 primary antibody was followed by incubation with 1 µg/ml Alexafluor633-conjugated streptavidin (ThermoFisher Scientific) for 1 hour before counterstaining with DAPI ...
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bioRxiv - Bioengineering 2022Quote: ... eBCs were stained with polyclonal Synaptotagmin-4 antibody (1:50; Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... incubated with anti-cytokine antibodies (IL-4 (11B11, BD and Invitrogen), IL-13 (eBio13A ...
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bioRxiv - Microbiology 2022Quote: ... and anti-mIgG-AF647 antibodies (4 μg/ml, Invitrogen #A-31571). Cell staining and washing were performed in PBS-0.1% BSA at 4°C ...
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bioRxiv - Physiology 2024Quote: ... were incubated overnight at 4°C and the secondary antibodies (Invitrogen) were incubated for 1 h at room temperature.
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bioRxiv - Cell Biology 2024Quote: ... for overnight at 4°C and Alexa-labeled secondary antibodies (Invitrogen, A11006 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4 μl of the Cy5 goat anti-rabbit antibody (Invitrogen, #A10523) and 4 μl of the Alexa Fluor goat anti-mouse antibody (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... were incubated overnight at 4°C and the secondary antibodies (Invitrogen) were incubated for 1 h at room temperature ...
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bioRxiv - Genetics 2021Quote: ... The membrane was hybridized with a biotin-conjugated telomere probe (5’-biotin-CACACCCACACCCACACC-3’) and was imaged using a Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Scientific) and a Li-Cor C-DiGit Chemiluminescent Western Blot scanner.
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bioRxiv - Developmental Biology 2021Quote: ... or 1mM CaCl2 with 10 mg/mL Biotin (EZ-Link™ Sulfo-NHS-LC-Biotin, No-Weigh Format; Thermo Fisher #A39257) for the contralateral experimental testis was injected ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 5 mg of membrane-impermeable sulfosuccinimidyl-6-(biotin-amido) hexanoate reagent at the concentration of 0.3 mg/mL (EZ-link sulfo-NHS-SS-biotin; 21331, Thermo Fisher Scientific) was applied to the cells with gentle shaking at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... the cell surface of exponentially growing cells was labeled with biotin by incubating the cells with 0.5 mg/ml Sulfo-NHS-LC biotin (#21335, Thermo Fisher scientific) for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed five times with lysis buffer containing 1 M NaCl and incubated with 25 µl of biotin elution buffer (lysis buffer with 5 mM D-biotin [Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Biotin-modification of aldolase (#28403842, Cytiva, Marlborough, USA) with EZ-Link™ Maleimide-PEG2-Biotin (#A39261, Thermo Fisher Scientific, Waltham, USA) was achieved through incubation at RT for 1 h and subsequent size-exclusion chromatography on a 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... This Cys residue was labeled with biotin by incubation of MSP with a 20-fold molar excess of maleimide-PEG11-biotin (ThermoFisher Scientific) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... from an overnight culture were washed with PBS and labeled with 8mg/ml sulfosuccinimidyl-6-[biotin-amido] hexanoate (Sulfo-NHS-LC-LC-Biotin, 21338, ThermoFisher Scientific) for 30 minutes at room temperature (RT) ...
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bioRxiv - Genomics 2022Quote: ... washed twice in sterile PBS and 300×106 cells were used for 30 min biotin labeling (5 mg/mL, EZ-Link Sulfo-NHS-LC-Biotin, Thermo Fisher). Excess Biotin was quenched with 0.1 M Glycine and cells were resuspended in sterile PBS before inoculation into fresh YPD medium containing 1 μM estradiol to initiate the Mother Enrichment Program ...
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bioRxiv - Cancer Biology 2020Quote: ... and nuclear extracts were incubated for 1.5 hr at 4°C with 1 µg of H3K4ox (ART-K(OH)-QTARKSTGGKAP-biotin) or irrelevant (AHIVMVDAYKPTK-NH(CH2)2NH-biotin) peptides that had been previously recovered with streptavidin magnetic beads (Invitrogen; 65305) for 30 min at 4°C ...
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bioRxiv - Genetics 2020Quote: AAG AGC TCT TCA GAA ATG GAT GCC ATG TGT GTC TCC TCT TCC TGA-3’) derived from the intron 10 of PIK3C2B were biotin-labeled using Biotin 3’-End DNA Labeling Kit (Thermo Scientific). Biotin-labeled ssODNs were then annealed with reverse complimentary ssODNs to generate biotin-labeled probes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... surface proteins were labeled with biotin by adding 1 mg/ml membrane impermeant EZ-LinkTM Sulfo-NHS-SS-Biotin (Thermo Fisher) in 137 mM NaCl and 15 mM sodium borate ...
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bioRxiv - Microbiology 2021Quote: ... ~108 CFU of the indicated strains grown to late log phase under competence-inducing conditions were washed in IO and incubated with 50 μg/mL biotin-maleimide (biotin-mal, Thermo Fisher) for 45 min at room temperature ...
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bioRxiv - Immunology 2020Quote: Ferric Hb and ferrous Hb were individually biotinylated using sulfo-NHS-LC-Biotin (Sulfosuccinimidyl-6- {biotin-amido} hexanoate; Thermo Fisher Scientific) and immobilized on streptavidin beads in spin columns (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Surface biotinylation was performed on using Sulfo-NHS-LC-LC biotin (Thermo, EZ-Link Sulfo NHS-LC-LC-Biotin) and streptavidin-agarose beads (Thermo Fisher). Samples were run on an 8% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2024Quote: ... 200 μg of total protein in a maximum volume of 50 μL was used for the Click-it reaction between Click-iT® HPG alkyne and Biotin Azide (PEG4 carboxamide-6-Azidohexanyl Biotin) (Invitrogen) was performed using Click-iT™ Protein Reaction Buffer Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Bait proteins were randomly conjugated to biotin and free biotin removed using EZ-Link Micro NHS-PEG4-Biotinylation Kit (ThermoFisher # 21955) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Single-stranded DNA oligonucleotide probes were synthesized by IDT and biotin end-labeled using the Pierce 3’ Biotin end-labeling DNA kit (Thermo Scientific) following manufacturer’s protocol with the following changes ...
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bioRxiv - Molecular Biology 2023Quote: ... The probes were purified and labelled with biotin by using the Biotin 30-End DNA Labeling Kit (Thermo Scientific, Rockford, USA)f ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and wells were washed twice with ice-cold PBS and labelled with 0.5 mg/ml biotin (EZ-Link sulfo-NHS-SS-biotin, Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... was biotinylated with 80-fold molar excess of NHS-PEG4-Biotin solution from the EZ-Link NHS-PEG4-Biotin kit (ThermoFisher, #A39259). Excess biotin was removed by buffer exchange of protein into sterile 1X PBS using Zeba Spin Desalting Columns ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was biotinylated (FXIIa-biotin) and the degree of labeling assessed using the EZ-Link Sulfo-NHS-LC-Biotin labelling kit (ThermoFisher Scientific) with 1-2 molecules of biotin per protein molecule ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were simultaneously incubated with 100 μM A-769662 (or DMSO) and either 1 μg/mL biotin-xx-Tfn or 5 ng/mL biotin-xx-EGF (both from Life Technologies) for the indicated timepoints at 37C ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with primary antibodies overnight at 4 °C (1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
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bioRxiv - Cell Biology 2021Quote: ... primary antibodies overnight at 4°C followed by Alexa Fluor-647 secondary antibodies (ThermoFisher Scientific, 1:500) for 2hrs at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were then applied at 4°C overnight in 1x PBS supplemented with Horse serum (4%, ThermoFisher, 16050114). The following synaptic antibodies were used ...
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bioRxiv - Physiology 2020Quote: ... Brain sections stained with guinea pig anti-tdTomato antibody (1:1,000; Frontier Institute) were incubated with biotin-labelled anti-guinea pig IgG (1:250; Invitrogen, Carlsbad, CA, USA) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... The FAP primary antibody was detected with a secondary biotin-conjugated anti-goat/sheep mouse IgG and 1:1000 Streptavidin PE-Cy7 (Thermo Fisher Scientific). EdU was detected using the Click-IT Plus Flow Cytometry Assay with AlexaFluor® 488 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by separation on 20% 7 M urea SDS-PAGE gel and immunoblot assay with anti-Biotin antibody (Thermo Fisher Scientific, 692033).
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with washing buffer (PBS containing 1%FBS) and incubated with biotin labelled anti-CD11b antibody (RM2815; Invitrogen; 1:100) for 40min with constant mixing using Hula mixer (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes from 3- to 4-week-old wild type mice were stained with biotinylated antibodies for lineage markers (excluding CD4) and depleted of lineage-positive cells by magnetic separation using Biotin Binder Dynabeads (Thermo Fisher Scientific). Flt3+ or Flt3-ETP were pre-sorted for yield and then 200 cells/well were sort-purified directly onto the OP9-DLL4 culture plate ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cell layers were washed gently with PBS once before addition of endogenous biotin blocking solutions (Endogenous Biotin Blocking kit, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... Northern blotting was conducted as our previously reported method [66] with biotin-labeled DNA probes using Biotin-11-dUPT (Thermo Scientific USA). High-molecular weight RNAs were separated in 1 % formaldehyde agarose gels and transferred to Hybond-N+ membranes (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... was biotinylated with 80-fold molar excess of NHS-PEG4-Biotin solution from the EZ-Link™ NHS-PEG4-Biotin kit (ThermoFisher, #A39259). Excess biotin was removed by buffer exchange of protein into sterile 1X PBS using Zeba™ Spin Desalting Columns ...