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Citations for Thermo Fisher :
351 - 400 of 7266 citations for Human Coronavirus NL63 Nuceloprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... coli (C737303; Invitrogen) cultures and verified with DNA digestion and gel electrophoresis before clonal expansion and purification with QIAGEN Plasmid Maxi Kit was performed (12163 ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (Invitrogen, 18265017) cells.
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bioRxiv - Evolutionary Biology 2024Quote: ... coli cells (ThermoFisher) using standard protocols ...
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bioRxiv - Genomics 2020Quote: ... and gel imager (E-Gel Safe Imager connected to E-Gel iBase, Invitrogen), according to the recommendations provided by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... of the SARS coronavirus strain Tor2 (NC_004718.3) was ordered as a synthetic DNA string fragment (GeneArt synthesis; Thermo Fisher Scientific, Darmstadt, Germany) and cloned into the pEXPR103 expression vector (IBA Lifesciences ...
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bioRxiv - Immunology 2021Quote: ... and coronavirus RBDs were expressed and purified from Expi293F cells cultured in 33% Expi293 Expression / 66% FreeStyle Expression medium (Thermo Fisher Scientific) and grown in baffled polycarbonate shaking flasks (Triforest ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated (1:1000 dilution) for 1 hour with a mouse anti-human E-Cad ectodomain antibody (HECD-131700, 1:1000 dilution; Life Technologies). After two washes ...
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bioRxiv - Immunology 2022Quote: A recombinant fragment of human SP-D (rfhSP-D) was expressed under bacteriophage T7 promoter in Escherichia coli BL21 (λDE3) pLysS (Invitrogen), transformed with for plasmid containing cDNA sequences for neck ...
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bioRxiv - Molecular Biology 2020Quote: The catalytic domain of the human ST6Gal I (residue range 132-406) was expressed in a BL21(DE3) Escherichia coli strain (Invitrogen), using a polycistronic Ptac vector containing the CyDisCo system (Gąciarz et al ...
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bioRxiv - Developmental Biology 2022Quote: ... donated foetal tissue stored in Hibernate-E medium (Hib-E, Thermo Fisher Scientific A1247601) was collected from local maternity hospital soon after passing ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then blocked with 5% dried milk in PBS-0.1%Tween followed by incubation with SARS Coronavirus NP Monoclonal Antibody (E16C) (ThermoFisher Scientific, Catalog # MA1-7403) or SARS Coronavirus Spike polyclonal Serum (BEI Resources ...
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bioRxiv - Microbiology 2023Quote: ... A stock Bovine Coronavirus (BCoV) vaccine was prepared by adding 3 mL of 1X Phosphate Buffered Saline (PBS; Fisher Scientific; Catalog # BP399-500) to one vial of lyophilized Zoetis Calf-Guard Bovine Rotavirus-Coronavirus Vaccine (Catalog # VLN 190/PCN 1931.20 ...
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bioRxiv - Immunology 2022Quote: ... E-cadherin (Invitrogen, PA5- 85088), EpCAM (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Cyclin E (Invitrogen, MA5-32358), GAPDH (GeneTex ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... William’s E Medium (Gibco, A1217601) supplemented with Primary Hepatocyte Maintenance Supplements (Gibco ...
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Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against InflammationbioRxiv - Cell Biology 2023Quote: ... E-selectin (Invitrogen #MA1-06506), ZO-1 (Invitrogen #MA3-39100-A488) ...
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bioRxiv - Cell Biology 2023Quote: ... E-cadherin (ThermoFisher, PA5-32178) 1:5000 ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA; anti E-Cadherin antibody: CD324 #16-3249-82, Invitrogen, USA) to bind to their respective epitopes ...
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bioRxiv - Biophysics 2021Quote: ... Coli TOP10 cells (Invitrogen) were used for plasmid propagation and isolation ...
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bioRxiv - Biophysics 2021Quote: ... Coli DH10Bac cells (Invitrogen). Recombinant baculoviruses were produced and amplified in Sf9 cells ...
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bioRxiv - Bioengineering 2020Quote: ... coli cells (Thermo Fisher). Cells were grown in 2xYT medium with appropriate antibiotics at 37°C with shaking until OD600 reached ∼ 0.4 – 0.6 ...
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bioRxiv - Cancer Biology 2021Quote: ... coli (Thermo Fisher Scientific) were transformed with the ligation product ...
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bioRxiv - Microbiology 2020Quote: ... coli cells (Invitrogen, UK). After transformation ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Thermo Fisher #C737303), and several colonies were picked up and sent for sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Thermo Fisher #C737303), and positive clones were screen by colony PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli (Thermo Fisher Scientific). Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... coli cells (Life Technologies). Cells were grown in LB broth to an OD600 of 0.7-0.8 and induced with 1mM IPTG for 2hr at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21Star (DE3, Invitrogen). Following cell disruption ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21Star (DE3, Invitrogen) and the complex purified by affinity chromatography using the chelating Ni2+ Sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (Life Technologies) and plated on ampicillin plates for selection ...
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bioRxiv - Molecular Biology 2020Quote: ... coli (Life Technologies, Invitrogen) and their cross-reactivity against GppNHp-bound KRAS determined by ELISA as previously described24.
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bioRxiv - Molecular Biology 2019Quote: ... coli expression (Life Technologies), and cloned into the pGAT3 vector for expression fused to an N-terminal His6-GST tag 64 ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli DH5α (Invitrogen) and SCS110 (Stratagene ...
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bioRxiv - Genomics 2020Quote: ... coli DNA ligase (Invitrogen), 0.3 μl 10 U/μl E ...
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bioRxiv - Evolutionary Biology 2019Quote: ... coli DH10B (ElectroMAX, Invitrogen).
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bioRxiv - Synthetic Biology 2019Quote: ... coli (Life Technologies, UK) was used for cloning ...
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bioRxiv - Plant Biology 2019Quote: ... coli (Thermo Fisher Scientific) and constructs were verified by sequencing (Eurofins Genomics).
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bioRxiv - Synthetic Biology 2019Quote: ... coli TOP10 cells (Invitrogen) were used for plasmid cloning and propagation ...
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bioRxiv - Systems Biology 2019Quote: ... coli cells (Invitrogen, #A10460) via heat shock bacterial transformation ...
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bioRxiv - Cell Biology 2020Quote: ... coli (Thermo Fisher Scientific) correctness of the integration was verified via sequencing (sequencing service ...
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bioRxiv - Biochemistry 2020Quote: ... coli competent cells (Invitrogen) were transformed following the recommended protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells (ThermoFisher Scientific) were transformed with pET-26b(+)-derived DNA constructs using heat shock ...
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bioRxiv - Plant Biology 2019Quote: ... coli (Thermo Fisher Scientific). Bacteria were grown overnight at 37°C on LB plates with 50 μg/mL kanamycin and plasmids from randomly selected positive clones were purified using QIAprep Spin Miniprep kit (QIAGEN ...
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bioRxiv - Neuroscience 2019Quote: ... coli strain (ThermoFisher, USA) to avoid ITR-mediated recombination ...
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bioRxiv - Cell Biology 2019Quote: ... coli BL21 (DE3) (Invitrogen). Cells were grown at 37°C to mid-log phase and induced with 1 mM IPTG for 2 hr ...
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bioRxiv - Biophysics 2019Quote: ... coli strain TOP10 (Invitrogen) was used as the host strain for all plasmids ...
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bioRxiv - Cancer Biology 2019Quote: ... coli strain (Thermo Fisher). Lentiviral particles were synthetized through transfection of 20µg of CAS9 expression vector ...
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bioRxiv - Biochemistry 2019Quote: ... coli Machl cells (ThermoFisher) were transformed with plasmid pLK35-P7A7-MS2 or pKK3535-040329-MS2 ...