Labshake search
Citations for Thermo Fisher :
351 - 400 of 1712 citations for Bordetella Pertussis Toxin Frozen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: The retrograde tracers were cholera toxin b subunit (CTb) conjugated to Alexa Fluor 488 (CTb-488) (Cat#C34775 ThermoFisher Scientific) or Alexa Fluor 555 (CTb-555 ...
-
bioRxiv - Neuroscience 2021Quote: ... pairwise injections of 80 nL of 0.5% (wt/vol) fluorescently labeled cholera toxin B subunit (CTB-488, ThermoFisher C22841, CTB-647, ThermoFisher C34788). In C57BL6/J mice ...
-
bioRxiv - Biophysics 2020Quote: ... The lipid ordered phase was visualized through GM1 crosslinking by incubating the PMS in 10 µg/ml of Alexa-488 labeled cholera-toxin subunit B (Invitrogen) for 2 h at 37°C and IFITM3-iSNAP was stained with 3 µM SNAP-cell 647-SIR (New England Biolabs ...
-
bioRxiv - Neuroscience 2020Quote: ... Cholera Toxin subunit-B (Luppi et al., 1995) with a Alexa Fluor-647 (AF-647) conjugate (ThermoFisher, catalog number C34778) was prepared by dissolving 100μg in 20μL PBS ...
-
bioRxiv - Neuroscience 2020Quote: The retrograde tracer cholera toxin subunit B (CTB) conjugated with Alexa Fluor™ 555 (CTB-555) was purchased from Invitrogen, Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibodies against binary toxin subunits CDTa and CDTb and anti-Clostridia GDH monoclonal mouse antibody (Clone: J44D, Invitrogen) were obtained from Merck ...
-
bioRxiv - Biophysics 2020Quote: ... DiIC18(1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotri-carbocyanine Iodide) and the Alexa 488-labeled fragment B of cholera toxin were purchased from Thermo Fisher scientific (Waltham ...
-
bioRxiv - Neuroscience 2022Quote: Projection neurons were retrograde labeled from their axon terminals by pressure injection of Alexa-conjugated Cholera Toxin B (Ctb, Invitrogen) or Fast Blue (Polysciences ...
-
bioRxiv - Neuroscience 2022Quote: ... SCPN (CBN + CSN) or CSN only were retrogradely labeled by bilateral injection of Cholera Toxin B subunit (CTB; Thermo Scientific) into either cerebral peduncles (at 3 injection sites ...
-
bioRxiv - Microbiology 2020Quote: ... GM1 coated microtiter plates were incubated with a 1:20 dilution of culture supernatant and detected using primary anti-cholera toxin and secondary HRP-conjugated goat anti-rabbit IgG (Invitrogen). 1-Step Ultra TMB-ELISA (Thermo Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... nwS1 was stereotaxically injected with 80-100 nl cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 (CTB-488, Invitrogen), and wS1 with CTB-Alexa Fluor 555 (CTB- 555 ...
-
Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections ...
-
bioRxiv - Neuroscience 2023Quote: P12 rats were deeply anesthetized with isoflurane and the sclera and cornea were pierced with a syringe containing a 1% solution of cholera toxin B subunit (Invitrogen) conjugated to either Alexa Fluor 488 (green ...
-
bioRxiv - Neuroscience 2023Quote: ... Drd1afl/fl mice received bilateral injections of Alexa Fluor 594-conjugated Cholera Toxin Subunit B in PBS (CTB-594, 1.0 mg mL−1, no. C-34776, Thermo Fisher) in either LH or VTA brain regions.
-
bioRxiv - Pathology 2023Quote: Vip3Aa toxin was biotinylated with a 1:30 (protein:biotin) molar ratio of EZ-Link Sulfo- NHS-LC-Biotin (Life Technologies) for 30 minutes on ice ...
-
bioRxiv - Bioengineering 2023Quote: ... the bacterial toxin ccdB was cloned with the enzymes KpnI and NheI using a dedicated commercial ccdB-resistant strain (ThermoFisher); the generated plasmid LentiCRISPRV1-gRNA-TetR-intron-ccdB was used to obtain the KpnI/NheI digested backbone for lentiviral library ...
-
bioRxiv - Neuroscience 2023Quote: ... regenerating axons were anterogradely labeled by intravitreal injection of 2 µl Alexa Fluor 555-conjugated cholera toxin β subunit (CTB, 0.5% in PBS; Invitrogen).
-
bioRxiv - Genomics 2023Quote: ... 0.5 mg/mL hydrocortisone (SigmaAldrich, #H0888), 100 ng/mL Cholera toxin (SigmaAldrich, #C8052, and 10 µg/mL insulin (ThermoFisher #12585014). To generate MCF10A cells stably expressing PiggyBac-NLS-ZapCV2 ...
-
bioRxiv - Neuroscience 2021Quote: ... Frozen sections were then mounted on SuperFrost Plus™ slides (Thermo Fisher). Gad1 In situ hybridisation on frozen sections was performed as previously described (Wallace and Raff ...
-
bioRxiv - Genetics 2021Quote: RNA was extracted from frozen liver using TRIzol reagent (Invitrogen, cat. # 15596026) following the manufacturer’s directions ...
-
bioRxiv - Genetics 2021Quote: Frozen tissue was homogenized in 1 ml of TRIzol reagent (Thermo Fisher) using a Bead Mill 24 homogenizer (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2021Quote: ... frozen samples were resuspended in 1 mL of Trizol (ThermoFisher; Cat #: 15596026) and subjected to three freeze-thaw cycles ...
-
bioRxiv - Microbiology 2020Quote: ... Imaging of frozen samples was performed on either a Titan (ThermoFisher Scientific) microscope equipped with a Gatan Ultrascan camera and operating at 300 kV or a Talos (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Total protein from frozen tissues was prepared with T-PER (ThermoFisher Scientific) supplemented with protease and phophatase inhibitors (Roche) ...
-
bioRxiv - Neuroscience 2020Quote: Snap frozen V1 tissues were homogenized in either TRIzol LS Reagent (ThermoFisher), or NP40 (ThermoFisher ...
-
bioRxiv - Neuroscience 2021Quote: ... and was frozen in 500 µl Trizol (Thermo Fisher, Cat. No. #15596026) on dry ice and stored at -80 °C until further processing ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue was then flash frozen with 2-methylbutane (O3551-4, Fisher Scientific) and stored at −75°C until sectioning.
-
bioRxiv - Neuroscience 2021Quote: Total RNA was prepared from flash frozen tissue with Trizol (Life Technologies), reversed transcribed with the high-capacity cDNA kit (Applied Biosystems) ...
-
bioRxiv - Pathology 2021Quote: ... Samples were snap-frozen in isopentane after cryoprotection with Cryomatrix (Thermo Scientific). For brain ...
-
bioRxiv - Neuroscience 2020Quote: ... Nerves were frozen-embedded in cryoprotectant medium (Thermo Fisher Scientific, Waltham, MA) in isomethylbutane at -80°C ...
-
bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from frozen root samples using TriZol (Ambion, USA) with DNase treatment according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were plunge frozen with a Vitrobot Mark IV (Thermo Fisher Scientific) with 100% humidity at 30°C and back-blotted for 3 to 5s.
-
bioRxiv - Cell Biology 2021Quote: Frozen grids were observed with a CorrSight inverted microscope (Thermo Fisher Scientific) using EC Plan-Neofluar 5x/0.16NA and EC Plan-Neofluar 40x/0.9NA air objectives (Carl Zeiss Microscopy) ...
-
bioRxiv - Neuroscience 2022Quote: ... Frozen samples were later homogenized in ice-cold RIPA buffer (#89901, ThermoFisher) supplemented with phosphatase inhibitors (PhosSTOP ...
-
bioRxiv - Systems Biology 2022Quote: ... frozen tissues were lysed and homogenized in T-Per (Thermo Fisher Scientific) with phosphatase and protease inhibitor (100 µl per 10 mg tissue ...
-
bioRxiv - Biochemistry 2022Quote: ... Grids were then plunge frozen using Vitrobot Mark IV (Thermo Fisher Scientific) with the following setting ...
-
bioRxiv - Biochemistry 2022Quote: ... Grids were then plunge frozen using Vitrobot Mark IV (Thermo Fisher Scientific) with the following setting ...
-
bioRxiv - Immunology 2022Quote: ... Snap-frozen samples were lysed in 10 mM CHAPS/PBS (AppliChem, Gibco) with 1x protease inhibitor (Roche) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Frozen embryos were thawed and lysed in 1 mL TRIzol (Life Technologies) and total RNA was extracted using the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... DRGs were either snap frozen or stored in RNAlater (Ambion, Austin, TX). The DPN donors include 2 males and 3 females (Age ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein was extracted from flash frozen tumors using a homogenizer (Fisher Scientific) in lysis buffer (1% Triton X-100 ...
-
bioRxiv - Bioengineering 2022Quote: ... and frozen in 90% heat inactivated FBS (ThermoFisher Scientific, Burlington, ON, Canada) + 10% DMSO (Sigma Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total RNA was extracted from frozen liver tissue with TRIzol reagent (Invitrogen) and reverse-transcribed using PrimeScript™ RT reagent kit (Takara BIO Inc. ...
-
bioRxiv - Physiology 2020Quote: RNA was extracted from frozen gastrocnemius lateralis muscles using TRIZOL (Invitrogen, USA) and homogenized with an automatic potter homogenizer (B ...
-
bioRxiv - Developmental Biology 2021Quote: ... Frozen flies were incubated with 1 mL of TRIzol Reagent (Life Technologies) and homogenized with a dounce ...
-
bioRxiv - Developmental Biology 2019Quote: Snap-frozen testis parenchyma was homogenised in TRIzol Reagent (Thermo Fisher Scientific) and RNA extracted following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: Flash frozen brains were placed in Trizol (Life Technologies, Grand Island, NY) and RNA was extracted according to manufacturer instructions ...
-
Maternal transmission of a paramutant phenotype requires intact DNMT2 functions in the male germlinebioRxiv - Genetics 2020Quote: RNAs were isolated from frozen sperm using mirVana miRNA Isolation Kit (Invitrogen). Bisulfite conversion of sncRNAs was conducted using the EZ RNA Methylation Kit (ZYMO Research) ...
-
bioRxiv - Immunology 2021Quote: The frozen cells were resuspended and washed in RPMI medium 1640 (Gibco). Anti-CD3 (BD ...
-
bioRxiv - Immunology 2020Quote: ... Isolated PBMCs were frozen in cell recovery Media containing 10% DMSO (GIBCO), supplemented with 90% heat-inactivated fetal bovine serum and stored liquid nitrogen before assays analyses.