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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for 7 7 Dimethyl 6 8 dioxaspiro 3.5 nonan 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... PE Cyanine 7 anti-mouse CD3 (Invitrogen, 565411), APC anti-mouse CD11b (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... on the QuantStudio 7 Pro system (ThermoFisher Scientific). Relative gene expression was calculated by the ΔΔ Ct method ...
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bioRxiv - Immunology 2024Quote: ... and a QuantStudio 7 Pro qPCR (Applied Biosystems) were used to quantify cDNA following manufacturer recommendations ...
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bioRxiv - Microbiology 2024Quote: ... For 7-AAD assays (Thermo Fisher, Cat#A1310), cells were harvested and washed twice with 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... on a ViiA 7 system (Thermo Fisher Scientific). ATP5O and TUBB3 were employed as calibrator genes ...
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bioRxiv - Cell Biology 2024Quote: ... Sept-7 1/800 (Thermo scientific, PA5-54755), GAPDH 1/5000 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cοtransfected with 7 μg CRISPR plasmid and 7 μg non-linearized HDR template using Lipofectamine 3000 according to the instructions (Thermo Fisher). 48 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... and the cell pellet was resuspended in annexin buffer with Annexin V-FITC and 7-AAD according to the manufacturer’s specifications (FITC Annexin V 7-AAD-Thermo Fisher®). Flow cytometry was performed using Attune flow cytometer (Thermo Fisher®) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with FACS buffer and incubated with the live-dead marker 7-aminoactinomycin D (7-AAD; Thermo Fisher Scientific) for 10 min at 4 °C in the dark ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis induction was determined by activation of Caspase 3 and 7 using the CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen).H460 cells were plated at 5 × 103 cells/well in black 96 well plates with clear bottoms (Costar ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... 100-µl drop of 0.5% low-melting agarose (FMC, Fig. 1D,E, Fig. S1G) containing a 6:7 ratio of spinal cord medium [MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... 2015) (Supplemental Table 6 and 7) junctions and a high-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, USA) and Sanger sequencing (Solgent ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 x 106 HEK293T17 cells were transfected on a 6-well plate with 7 µl Lipofectamine 3000 (Thermo Fisher Scientific), the packaging plasmids psPax2 (3 µg ...
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bioRxiv - Cancer Biology 2023Quote: ... following manufacturer’s protocols and gene expression quantified via Applied Biosystems Quant Studio 6/7 Real-Time PCR System (Applied Biosystems), for TaqMan (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... BHK-21 cells (7×105/well) were transfected in tissue culture-treated 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) with the LCMV Arm plasmids pCAGGS-NP (0.8 µg) ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Biophysics 2023Quote: ... the media was removed and the cells were incubated with 8 µM CellEventTM Caspase 3-7 green detection reagent (Thermo Fisher) in PBS containing 5% FBS for 30 min at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... for 1h at 37°C or 2 μM CellEvant CASPASE-3/7 Green detection reagent (Thermo Fisher) for 30 minutes at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... The surface was incubated with 7 μL of a 2 mg/ml neutravidin solution (Thermo Fisher Scientific). Excess neutravidin was then washed off with 100 μL of 1X PBS buffer.
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bioRxiv - Biophysics 2020Quote: COS-7 (ATCC, CRL-1651) and HeLa (ATCC, CCL-2) cells were grown in DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... or 10 μM 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) succinimidyl ester (DDAO-SE) (Invitrogen) in HMI11 and incubated for 20 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The bacterial cell pellets were resuspended in 1X PBS and 2’,7’-dichlorofluorescein diacetate (Thermo Fisher Scientific) was added at a final concentration of 100 µM ...
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bioRxiv - Microbiology 2023Quote: Intracellular ROS levels were quantified using the redox-sensitive dye 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Thermofisher Scientific). Exponentially growing PA14 cultures were left untreated or treated with 0.25 µg/ml Gm ...
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bioRxiv - Biophysics 2023Quote: ... The reaction mixture was then transferred to 2 ml ZebaSpin MWCO 7 kD (Thermo Fisher Scientific, USA) columns to separate the labeled protein from the free dye ...
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bioRxiv - Biophysics 2023Quote: The cells were incubated with nonfluorescent cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, 5μM, ThermoFisher Scientific, UK) probe for 30 min prior to ES ...
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bioRxiv - Microbiology 2023Quote: ... LLC-MK-2 cells (rhesus monkey kidney; CCL-7/ATCC) were cultured in MEM (GIBCO #11095-080) supplemented with 100 units/ml penicillin and 100 μg/ml streptomycin (P/S) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained at 37°C for 30 min with 2 µM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCF-DA; Thermo Fisher Scientific) or 1 µM MitoSOX (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
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bioRxiv - Cancer Biology 2019Quote: ... T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher, C10423) in 96 well plates ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then resuspended in aCSF and incubated with DAPI (1:1000 dilution of 1mg/mL DAPI, Thermo Fisher, #62248 or 7-AAD 7-Aminoactinomycin D, A1310, Thermo Fisher) for FACS sorting ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... The nucleoids were subsequently stained for 5 min at room temperature with 7-amino-actinomycin D (1 mg/ml 7-AAD in DMSO, Thermo Fisher Scientific) diluted 1:400 in PBS buffer ...
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bioRxiv - Microbiology 2023Quote: ... The qPCR reactions for the data corresponding to Figure 7 and Figure 7 – figure supplement 1 were carried out in 384-well plates on a QuantStudio™ 5 (Applied Biosystems). The thermal cycling conditions were as follows ...
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bioRxiv - Microbiology 2023Quote: ... and undigested chromatin were adjusted to 810μl with MCN-digestion buffer with 0.005M EGTA and cross-linked by adding 135 μl of buffered 7 % formaldehyde solution (7 % CH2O, 140 mM Na2HPO4 pH 8.67) using 16 % CH2O methanol-free ampules (Thermo Scientific Cat #28906), to a final concentration of 1 % ...
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bioRxiv - Cancer Biology 2021Quote: ... QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher).
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bioRxiv - Cancer Biology 2021Quote: ... in ViiA 7 Real-time PCR Instrument (Applied Biosystems). The Standard deviation calculation and quantification were done from three independent experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-Claudin-7 (Invitrogen, 34-9100, Carlsbad, CA, USA), anti-VDR (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: ... Human hepatocellular carcinoma cells (Huh-7; Thermo Fisher Scientific) and African green monkey kidney cells (Vero E6 ...
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bioRxiv - Developmental Biology 2021Quote: ... on a ViiA 7 real-time PCR System (ThermoFisher). The list and sequences of the primers used are presented in Table S1 ...
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bioRxiv - Microbiology 2020Quote: ... containing 7% gamma-irradiated fetal bovine serum (FBS, Gibco). SS2-1 stored in 50% glycerol was kept in JAAS-IVM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... for 7 min and washed in PBS (Thermo Fisher Scientific ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... for 7 minutes to a nitrocellulose membrane (Invitrogen, IB301002). Membranes were subsequently blocked with a 5% milk solution in tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Plant Biology 2019Quote: ... in ViiA 7 Real-Time PCR System (Applied Biosystems). Relative expression was calculated in enzyme or 17-β-estradiol treated leaves with respect to mock/control (buffer or 0.1% DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... then for 7 days in Neurobasal Medium (Gibco, 21103049) supplemented with 1% GlutaMAX (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained with 7-AAD (ThermoFisher Scientific, #A1310) and AnnexinV-PE (BioLegend ...