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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for 6H Pyrano 3 2 g benzothiazole 8CI 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The lungs were inflated with 1-3 ml 2% UltraPure Low Melting Point Agarose (Invitrogen). Lungs and livers were fixed overnight at 4°C on a shaker ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were aspirated and 2-3 crystals of Dil stain (Life Technologies-Molecular Probes) were added to each culture well and incubated on a shaker for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were aspirated and 2-3 crystals of Dil stain (Life Technologies-Molecular Probes) were added to each culture well and incubated on a shaker for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Passaging was performed every 2 to 3 days using Accutase (00-4555-56, ThermoFisher Scientific). The cells were Mycoplasma tested prior to running experiments ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was carefully removed and resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 20 min at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were subcultured every 2-3 days and transfected with lipofectamine 2000 transfection reagent (Invitrogen) in Opti-MEM medium (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 ng equivalent was synthesized using Super Script 3 Supermix (18080400, Thermo Fisher, USA) with stem-loop primers and sly actin_R primers as internal controls (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 2–3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer ...
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bioRxiv - Genomics 2023Quote: ... mESCs were routinely passaged at ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher). Before experiments ...
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10-3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days by detachment with 0.05% trypsin-EDTA (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... Cytotoxicity was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher: M6494) reduction analysis according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Organoid images were digitally acquired every 2–3 days on an EVOS M7000 (ThermoFisher, AMF7000) with DiamondScope software (version 2.0.2094.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... passage 2-3 NPCs were detached with Stempro Accutase cell dissociation reagent (A1110501, Life Technologies) and kept in cold PBS 1%FBS for the duration of the protocol ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Neuroscience 2020Quote: ... 9.4% (1.023 g/ml) and 7% (1.017 g/ml) OptiprepTM (1.320 g/ml) (Thermo Fisher Scientific). After centrifugation at 800 x g for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Frozen tissues (3 - 5 mm3, 0.1 - 0.3 g) were submerged in 10 ml of TRIZOL Reagent (Life Technologies, Carlsbad, CA, USA), an amount three times larger than that recommended by the supplier ...
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bioRxiv - Biochemistry 2019Quote: ... cells were cultured for 48h in the absence and for 3 weeks in the presence of 1 mg/mL G-418 selection antibiotic (Thermo Fisher). Stable cells expressing GFP-fusion proteins were trypsinized and single GFP+cells with scatter properties (FSC and SSC ...
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bioRxiv - Immunology 2019Quote: ... 15 μg of pMul1-FLAG and 15 μg of pAsc-Myc mixed with FuGENE HD at a ratio of 1:3 in 500 μl Opti-MEM (Thermo Fisher) were transfected ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated cells were collected by centrifugation (200 g, 3 min at 25 °C) and resuspended in complete medium comprising neurobasal medium (Gibco, 21103) supplemented with 2% B-27 plus (Gibco A3582801) ...
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bioRxiv - Microbiology 2020Quote: Parasites after treatment were washed in 5 mL complete medium at 400 g for 3 min and stained with 5 μM JC-1 (ThermoFisher Scientific) in complete medium in the dark at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 more times in PBS for 10 minutes each and mounted on cover slides with Floromount G (Thermo Fisher Scientific). Slips were imaged an Olympus VS120 slide scanning microscope.
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Genomics 2022Quote: The following steps were conducted in 0.2 mL tubes (Axygen Biosciences): 3 μL of D2 buffer from a REPLI-g Single Cell Kit (Thermo Fisher Scientific) was added to 4 μL of droplet suspension ...
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bioRxiv - Genomics 2019Quote: ... Six μg of the isolated RNA was used for 5’- and 3’ RACE reactions with a FirstChoiceTM RLM-RACE Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... 2.5 µg of freshly SEC-purified MBP-RIC-3-His6 (bait) was added to 15 µL of HisPurTM Cobalt resin (Thermo Scientific) equilibrated with and re-suspended in 200 µL of binding buffer (Buffer A ...
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bioRxiv - Cell Biology 2019Quote: ... 1 µM TSO (5’- AAGCAGTGGTATCAACGCAGAGTACATrGrG+G-3’, Exiqon as described in (Picelli et al., 2014) and UltraPure DNase/RNase-Free Distilled Water (Invitrogen, 10977035). The Smart-seq2 samples were centrifuged for 700 g for 10 seconds and then placed in a thermal cycler ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA (2 μg of each plasmid) and FuGENE (3 μL FuGENE/μg DNA) were mixed thoroughly in 100 μL Opti-MEM (Thermo Fisher) and incubated at room temperature for 15 min ...
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bioRxiv - Genomics 2021Quote: ... 5 × 106 cells were plated in 10 cm dishes and co-transfected with 3 μg VSV-G plasmid and 9 μg of the respective construct Lipofectamine LTX (Thermo Fisher) the following day ...
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bioRxiv - Immunology 2021Quote: ... Coverslips were washed 3 times and mounted on slides using Fluoromount-G™ mounting medium with DAPI (Thermofisher; 00-4959-52). Images were acquired on an SP5 confocal microscope and analysed using ImageJ software.
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bioRxiv - Evolutionary Biology 2022Quote: ... A poly(G)-tail was added at the 3’ end of the RNA according to a Poly(A) Polymerase protocol (Thermo Scientific), using GTP instead of ATP ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (3 μg for ChIP-qPCR and 9 μg for ChIP-seq) were coupled to protein G dynabeads (Thermo Fisher Scientific) for 6 hours at 4°C and then incubated with fragmented chromatin over night at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting membrane pellet was resuspended in buffer M10 at 3 mL/g (of starting mitochondria) and the protein concentration was determined using a BCA assay (Pierce Thermo Fisher). The resuspended membranes were stored at 10 mg/mL of total protein at -80°C in a final glycerol concentration of 30% (v/v ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted again and resuspended in 3 × 106 cells/mL before adding CC4F-AM (LiveBLAzer FRET-B/G Loading Kit with CCF4-AM, ThermoFisher Scientific). Cells were incubated for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Neuroscience 2023Quote: ... To revive hiPSCs from cryopreservation cells were thawed by incubation for 2 minutes at 37°C and retrieved by centrifugation (200 x g for 3 minutes) prior to resuspension in Essential 8 (E8, (A1517001, Life Technologies) medium with 1’RevitaCell ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (0.5 μg/cm2) and Lipofectamine 2000 (1 μg RNA:3 μL reagent) were added separately to 50 μL opti-MEM (Thermo Fisher) and incubated for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting supernatant was mixed with 0.25% G-250 Sample Additive and loaded on a NativePAGE 3-12% Bis-Tris gel (BN1001BOX, Invitrogen, CA, USA) for electrophoresis.
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bioRxiv - Immunology 2023Quote: ... slides were washed 3 times with 0.2% Tween-20 in PBS and then mounted in Fluoromount G (Invitrogen, 00-4958-02). For signal amplification of CD163 and multiplexing with primary antibodies from the same species (Rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... hPSCs were centrifuged at 300 x g for 3 min and resuspended in 500 µL accutase (ThermoFisher Scientific, #00-4555-56) for 3 min at RT ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sections were washed again in PBST 3 times and were mounted using Fluoromount-G with DAPI (Thermo Fisher Scientific, Waltham, MA). They were imaged on a BZ-X800 Analyzer Keyence fluorescence microscope (Keyence ...
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bioRxiv - Molecular Biology 2024Quote: ... After centrifugation at 300 x g for 3 minutes the pellet containing embryonic neurons was resuspended in cortical neurons growth media: Neurobasal (Gibco 21103049), B27 Supplement (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... Coverslips were stained with 2 μg/mL of DAPI for 10 min and mounted with Fluoromount-G (ThermoFisher). Stained slides were imaged using the Leica HyVolution SP8 gated STED Microscope and manually scored for co-localization events and cell count using LasX Software.
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bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2 µl of RNAPII S5P (61085, Actif motif) and 20 µl Protein-G-Dyna beads (Life Technologies) were used per ChIP ...
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bioRxiv - Bioengineering 2021Quote: ... CRL-11268) with a pHR’SIN:CSW transfer vector and psPAX.2 and pMD2.G packaging plasmids using Lipofectamine 3000 (Invitrogen). 24 hr post transfection ...
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bioRxiv - Immunology 2020Quote: ... the collected supernatant was centrifuged at 120,000 × g for 2 h with an ultracentrifuge (Thermo Scientific Sorvall WX100), and the contents at the bottom of the centrifuge tube were resuspended in 500 μL of PBS ...
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bioRxiv - Biochemistry 2020Quote: ... 2 g/L Bovine Serum Albumin (BSA, Fischer Scientific) and 200 mg/L Salmon Sperm DNA solution (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... goat anti-Synaptopodin 2 µg) or control IgG coupled to Protein G-coupled magnetic beads (Dynabeads, Life Technologies). Unbound material was removed and the beads washed three times with homogenization buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2 diluted whole cell extract in buffer C was added to Dynabeads Protein G (Thermo Fisher Scientific) pre-incubated with HEXIM1 antibody ...