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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for Recombinant Rabbit IFNB1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant PrP substrates containing the D178N or E200K mutations were created by site-directed mutagenesis with the GeneTailor system (Invitrogen) using the bank vole (BV ...
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bioRxiv - Microbiology 2023Quote: ... and intracellular staining was performed using recombinant hu-mouse anti SeV-NP antibody conjugated with AlexaFluor-647 (1:1000) (Invitrogen) in combination with either anti-Krt5 (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of the reverse transcription reaction was used to amplify regions of interest using gene-specific primers in a 50 µL PCR reaction using recombinant Taq DNA Polymerase (Invitrogen) (see Table S2) ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2 x 106 cells/mL in Lonza Insect-XPRESS medium supplemented with 0.1 mM biotin ...
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bioRxiv - Biophysics 2023Quote: ... These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen, Catalog # 10359016).
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Neuroscience 2023Quote: ... Each tissue was homogenized in 500 μL of lysis buffer containing 400 units of RNase inhibitor (RNaseOUT Recombinant Ribonuclease Inhibitor, 10777-019, Invitrogen) and 0.1% Triton-X100 in low sucrose buffer (0.32 M sucrose ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant plasmid and the empty pBI121 vector underwent digestion using the restriction enzymes XbaI and SacI (Thermo Scientific TM). The purified digested insert and vector ligated using T4 DNA ligase at a 3:1 ratio ...
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bioRxiv - Microbiology 2023Quote: Recombinant baculoviruses (AcNPV) were generated using pFastBac1 AcGFP-JEVcore-FOS and a Bac-to-Bac system (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced using random hexamer primers (Mycrosynth) with recombinant M-MuLV reverse transcriptase (RevertAid, Thermo-Fisher Scientific, Cat #K1691) and used for quantitation of TTP-bound RNA and total RNA by RT-qPCR with HOT FIREPol EvaGreen qPCR Supermix (Medibena ...
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bioRxiv - Microbiology 2024Quote: ... 1μg recombinant PolyHis-p62 was adsorbed to 5 μl pre-equilibrated packed Ni-NTA resin (Thermo Fisher Scientific, Cat# 88221) via 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen). After digestion ...
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bioRxiv - Immunology 2024Quote: ... or 50 ml of 0.5 mg/ml MSP2 recombinant antigen (McCarthy et al., 2011a) in PBS were coated to 96-well flat bottom MaxiSorb plates (Nunc) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit and rat and Alexa Fluor 532 conjugated to antibodies raised against mouse and rabbit (Invitrogen) were used at 1/200 dilution in the saturation media ...
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bioRxiv - Neuroscience 2019Quote: ... Milipore) and a secondary Alexa Fluor 546 goat-anti-rabbit (1:400 anti-rabbit, Molecular probes) using an antibody diluent (Zytomed Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 680 donkey anti-rabbit or Alexa Fluor 800 donkey anti-rabbit (1:10,000; Invitrogen). To confirm equal protein loading ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit cardiac fibroblasts were treated with rabbit fibroblast media supplemented with either dimethylsulfoxide (DMSO, Thermo Fisher) vehicle or Etoposide (MedChemExpress) ...
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bioRxiv - Cancer Biology 2021Quote: ... and secondary antibody α-rabbit (1:5000, goat anti-rabbit IgG DyLight800 SA5-35571, Invitrogen, USA). Signal detection was performed with the LI-COR Odyssey Infrared Imaging System (LI-COR Bioscience ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LAMP1 was stained with rabbit anti-LAMP1 and anti-rabbit Alexa Fluor 568 (Thermo Fisher Scientific).
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: ... a rabbit anti-FHV polyclonal antibody and an Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen) were used to probe FHV alpha ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-rabbit-A647 (ref. A31573) and anti-rabbit-A488 (ref. A21206) were from Molecular Probes (Invitrogen). Alexa-conjugated antibodies A568 (ref ...
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bioRxiv - Cell Biology 2024Quote: ... AF488 donkey anti Rabbit IgG and AF647 polyclonal donkey anti-rabbit IgG antibody (all from Invitrogen) were used.
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bioRxiv - Neuroscience 2023Quote: ... tryptophan hydroxylase 2 (primary: rabbit anti-TpH, Novus #NB100-74555; secondary: goat anti-rabbit 546, Invitrogen #A-11035 or goat-anti rabbit 405 ...
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody against rabbit (Alexa-Fluor 488, donkey anti rabbit IgG, 1:1000, Invitrogen (Thermofisher) A21206 ...
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody against rabbit (Alexa-Fluor 488, donkey anti rabbit IgG, 1:1000, Invitrogen (Thermofisher) A21206 ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 555 goat anti-rabbit (1:500, Invitrogen). After mounting ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were then transferred into fresh Eppendorfs and frozen at −80°C (for radiolabeled glucose to protein) or their protein concentrations calculated (Western blot) using a Pierce BCA Protein Assay kit (Thermo Scientific, Cat#23225, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... pellets were reconstituted in 50[µl of 0.1[N NaOH and kept for protein quantification using a BCA protein assay kit (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). Dialysate concentrations are expressed as ng/ml and tissular concentrations as ng/mg of total proteins.
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bioRxiv - Neuroscience 2023Quote: Total protein levels in cortex homogenates were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Protein amounts were determined using a Qubit4 Fluorometer and Qubit Protein Assay kit (Thermo Fisher Scientific). GUS activity was calculated in (nmol 4-MU ...
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bioRxiv - Microbiology 2020Quote: ... Lysate protein concentrations were determined using a BCA protein assay kit according to manufacturer’s instructions (ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was collected for protein quantification using Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Developmental Biology 2020Quote: Changes in protein expression were monitored using Click-iT Protein Synthesis Assay kit (Thermo Fisher Scientific). Oocytes and 2 cell embryos were cultured in M16 medium with the methionine analogue HPG at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... and DNA-protein complexes were pulled down using Protein A/G agarose beads (Thermo Fisher, 20423). Agarose beads were washed with the following buffers ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined in the supernatant by BCA Protein Assay Kit (Thermo Fisher Scientific, #23227). 100 μg of protein was transferred into a new tube and the final volume was adjusted to 100 μL with 100 mM triethylammonium bicarbonate (TEAB) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants were collected for measuring the protein concentration with BCA Protein Assay Kit (ThermoFisher Scientific). Cytosolic and nuclear extracts were prepared using NE-PER reagents (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the supernatant measured using the Pierce BCA protein assay kit (Thermo Scientific) was used as a control.
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bioRxiv - Immunology 2021Quote: ... and sample protein concentration determined using the Pierce® BCA Protein Assay Kit (Thermo scientific, UK). SDS-PAGE was then performed ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were mixed with 20 μL Protein G-conjugated magnetic beads (DB10009, Thermo Fisher Scientific) with 2.0 μg antibody ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentration for each sample was determined using the Pierce BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Supernatants were collected and protein quantification was performed using a BCA protein assay kit (Thermo Scientific). A total of 2,000 μg of protein extract was used to perform the immunoprecipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... The extracted proteins were quantified by Pierce™ Modified Lowry Protein Assay Kit (Thermo Fisher Scientific) prior to precipitation by the phenol/chloroform method (Wessel and Flügge 1984) ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate total protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific, 23225).
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bioRxiv - Evolutionary Biology 2021Quote: ... Total protein extracts were prepared by using B-PER® Bacterial Protein Extraction Reagent (Thermo Scientific) as per manual ...
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bioRxiv - Neuroscience 2020Quote: ... The protein concentration of pooled triplicates was determined using the BCA protein assay (Thermo Fisher Scientific) and 8 μg of total protein subjected to immunoblotting against cleaved caspase-3 and −7 (1:200 dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific, Rockford, IL, USA). Samples were prepared for SDS-PAGE in Laemmli sample buffer (Bio-Rad ...