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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... High titre (>3 × 108 viral particles per ml) recombinant baculovirus was obtained using the Bac-to-Bac expression system (Invitrogen) in Sf9 (Spodoptera frugiperda ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant baculovirus generation and large-scale expression in Sf9 cells were carried out using the Bac-to-Bac™ (Invitrogen) expression system.
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bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic fragments were further shuffled into the pLenti-Gateway vector via Gateway LR recombinant reaction using Gateway LR Clonase II Enzyme Mix (Invitrogen). In the case of KCNA2 and OVOL1 ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Microbiology 2023Quote: Recombinant baculoviruses (AcNPV) were generated using pFastBac1 AcGFP-JEVcore-FOS and a Bac-to-Bac system (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Each tissue was homogenized in 500 μL of lysis buffer containing 400 units of RNase inhibitor (RNaseOUT Recombinant Ribonuclease Inhibitor, 10777-019, Invitrogen) and 0.1% Triton-X100 in low sucrose buffer (0.32 M sucrose ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of the reverse transcription reaction was used to amplify regions of interest using gene-specific primers in a 50 µL PCR reaction using recombinant Taq DNA Polymerase (Invitrogen) (see Table S2) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant PrP substrates containing the D178N or E200K mutations were created by site-directed mutagenesis with the GeneTailor system (Invitrogen) using the bank vole (BV ...
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bioRxiv - Microbiology 2023Quote: ... and intracellular staining was performed using recombinant hu-mouse anti SeV-NP antibody conjugated with AlexaFluor-647 (1:1000) (Invitrogen) in combination with either anti-Krt5 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2 x 106 cells/mL in Lonza Insect-XPRESS medium supplemented with 0.1 mM biotin ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen, Catalog # 10359016).
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bioRxiv - Microbiology 2024Quote: ... 1μg recombinant PolyHis-p62 was adsorbed to 5 μl pre-equilibrated packed Ni-NTA resin (Thermo Fisher Scientific, Cat# 88221) via 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced using random hexamer primers (Mycrosynth) with recombinant M-MuLV reverse transcriptase (RevertAid, Thermo-Fisher Scientific, Cat #K1691) and used for quantitation of TTP-bound RNA and total RNA by RT-qPCR with HOT FIREPol EvaGreen qPCR Supermix (Medibena ...
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bioRxiv - Microbiology 2024Quote: ... while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen). After digestion ...
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bioRxiv - Immunology 2024Quote: ... or 50 ml of 0.5 mg/ml MSP2 recombinant antigen (McCarthy et al., 2011a) in PBS were coated to 96-well flat bottom MaxiSorb plates (Nunc) overnight at 4°C ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit and rat and Alexa Fluor 532 conjugated to antibodies raised against mouse and rabbit (Invitrogen) were used at 1/200 dilution in the saturation media ...
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bioRxiv - Neuroscience 2019Quote: ... Milipore) and a secondary Alexa Fluor 546 goat-anti-rabbit (1:400 anti-rabbit, Molecular probes) using an antibody diluent (Zytomed Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 680 donkey anti-rabbit or Alexa Fluor 800 donkey anti-rabbit (1:10,000; Invitrogen). To confirm equal protein loading ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit cardiac fibroblasts were treated with rabbit fibroblast media supplemented with either dimethylsulfoxide (DMSO, Thermo Fisher) vehicle or Etoposide (MedChemExpress) ...
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bioRxiv - Cancer Biology 2021Quote: ... and secondary antibody α-rabbit (1:5000, goat anti-rabbit IgG DyLight800 SA5-35571, Invitrogen, USA). Signal detection was performed with the LI-COR Odyssey Infrared Imaging System (LI-COR Bioscience ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LAMP1 was stained with rabbit anti-LAMP1 and anti-rabbit Alexa Fluor 568 (Thermo Fisher Scientific).
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: ... a rabbit anti-FHV polyclonal antibody and an Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen) were used to probe FHV alpha ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-rabbit-A647 (ref. A31573) and anti-rabbit-A488 (ref. A21206) were from Molecular Probes (Invitrogen). Alexa-conjugated antibodies A568 (ref ...
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bioRxiv - Cell Biology 2024Quote: ... AF488 donkey anti Rabbit IgG and AF647 polyclonal donkey anti-rabbit IgG antibody (all from Invitrogen) were used.
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody against rabbit (Alexa-Fluor 488, donkey anti rabbit IgG, 1:1000, Invitrogen (Thermofisher) A21206 ...
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody against rabbit (Alexa-Fluor 488, donkey anti rabbit IgG, 1:1000, Invitrogen (Thermofisher) A21206 ...
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bioRxiv - Neuroscience 2023Quote: ... tryptophan hydroxylase 2 (primary: rabbit anti-TpH, Novus #NB100-74555; secondary: goat anti-rabbit 546, Invitrogen #A-11035 or goat-anti rabbit 405 ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 555 goat anti-rabbit (1:500, Invitrogen). After mounting ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were then transferred into fresh Eppendorfs and frozen at −80°C (for radiolabeled glucose to protein) or their protein concentrations calculated (Western blot) using a Pierce BCA Protein Assay kit (Thermo Scientific, Cat#23225, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: Total protein levels in cortex homogenates were quantified using a Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... pellets were reconstituted in 50[µl of 0.1[N NaOH and kept for protein quantification using a BCA protein assay kit (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). Dialysate concentrations are expressed as ng/ml and tissular concentrations as ng/mg of total proteins.
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bioRxiv - Plant Biology 2020Quote: ... Protein amounts were determined using a Qubit4 Fluorometer and Qubit Protein Assay kit (Thermo Fisher Scientific). GUS activity was calculated in (nmol 4-MU ...
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bioRxiv - Microbiology 2020Quote: ... Lysate protein concentrations were determined using a BCA protein assay kit according to manufacturer’s instructions (ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was collected for protein quantification using Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Developmental Biology 2020Quote: Changes in protein expression were monitored using Click-iT Protein Synthesis Assay kit (Thermo Fisher Scientific). Oocytes and 2 cell embryos were cultured in M16 medium with the methionine analogue HPG at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... and DNA-protein complexes were pulled down using Protein A/G agarose beads (Thermo Fisher, 20423). Agarose beads were washed with the following buffers ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined in the supernatant by BCA Protein Assay Kit (Thermo Fisher Scientific, #23227). 100 μg of protein was transferred into a new tube and the final volume was adjusted to 100 μL with 100 mM triethylammonium bicarbonate (TEAB) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants were collected for measuring the protein concentration with BCA Protein Assay Kit (ThermoFisher Scientific). Cytosolic and nuclear extracts were prepared using NE-PER reagents (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the supernatant measured using the Pierce BCA protein assay kit (Thermo Scientific) was used as a control.
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bioRxiv - Immunology 2021Quote: ... and sample protein concentration determined using the Pierce® BCA Protein Assay Kit (Thermo scientific, UK). SDS-PAGE was then performed ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were mixed with 20 μL Protein G-conjugated magnetic beads (DB10009, Thermo Fisher Scientific) with 2.0 μg antibody ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentration for each sample was determined using the Pierce BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Supernatants were collected and protein quantification was performed using a BCA protein assay kit (Thermo Scientific). A total of 2,000 μg of protein extract was used to perform the immunoprecipitation ...