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Citations for Thermo Fisher :
3901 - 3950 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... + 500 U/mL Gibco™ recombinant human (rh) IL-2 (Thermo Fisher Scientific, cat# PHC0021). For primary NK cells ...
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bioRxiv - Bioengineering 2024Quote: ... adding 5 mM GMP and 4 U/µL RNaseOUTTM recombinant ribonuclease inhibitor (Thermo Fisher Scientific) to the transcription reaction ...
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bioRxiv - Bioengineering 2024Quote: ... and 100U/ml PeproGMP™ Human IL-2 Recombinant Protein (Thermo Fisher; 200-02-1MG). The T cell culture was transferred to G-Rex bioreactor vessels (Wilson Wolf ...
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bioRxiv - Neuroscience 2024Quote: Cholera toxin subunit B (recombinant) (CTb) Alexa Fluor 488 (Cat#C34775, Therma Fisher Scientific, USA) and CTb Alexa Fluor 555 (Cat#CC34776 ...
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bioRxiv - Biochemistry 2024Quote: ... four high-titer recombinant baculoviruses were used for co-infection of High Five cells (ThermoFisher). After 48 hours infection ...
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bioRxiv - Neuroscience 2024Quote: ... The Sf21 insect cells were transfected with recombinant bacmid using Cellfectin reagent (Thermo Fisher Scientific) to obtain recombinant baculovirus ...
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bioRxiv - Microbiology 2020Quote: ... Ethanolamine was then removed from the e-pili samples with dialysis tubing (Slide-A-Lyzer, 2KDa, ThermoFisher) suspended in deionized water ...
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bioRxiv - Synthetic Biology 2021Quote: ... Annealing efficiency was confirmed by visualizing the dsDNA on a 4% EX Agarose E-Gel (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genetics 2021Quote: ... Membranes were imaged using MicroChemi (DNR Bio-Imaging Systems) and E-Gel Imager GelCapture Acquisition System (ThermoFisher).
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bioRxiv - Neuroscience 2020Quote: ... DNA size selection was performed after PCR amplification using E-Gel Precast Agarose Electrophoresis System (Invitrogen #A42100). Samples were sequenced single-end using 50 bp reads on the HiSeq-2500 and Hi-Seq 4000 platforms (Illumina ...
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bioRxiv - Cell Biology 2019Quote: ... and the cells were grown in collagen-coated plates with William’s Medium E (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Cell Biology 2019Quote: ... and the cells were grown in collagen-coated plates with William’s Medium E (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Genomics 2019Quote: ... Differentiating cells (4.4 × 104/cm2) were grown for 1 week in William’s E medium (Gibco 12551-032) supplemented with 10% fetal bovine serum (Eurobio CVFSVF0001) ...
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bioRxiv - Immunology 2019Quote: ... resuspended in William’s E medium containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific) and plated ...
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bioRxiv - Developmental Biology 2020Quote: ... and anti-E-cadherin (rat, 1:50; formaldehyde-hand peeled fixation; DHSB) antibodies and AlexaFluor568 phalloidin (Invitrogen) were used ...
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bioRxiv - Bioengineering 2020Quote: ... Hematoxylin and eosin (H&E) staining was performed on the Gemini autostainer (Thermo Fisher Scientific, Waltham, MA). Safranin O staining was performed by hand using a kit (StatLab).
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bioRxiv - Cancer Biology 2021Quote: ... Hematoxylin and eosin (H&E) staining was performed using Mayer’s hematoxylin solution and Eosin Y (Thermo Fisher). IHC slides were then scanned on a Pannoramic SCAN scanner (Perkin Elmer) ...
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bioRxiv - Systems Biology 2020Quote: ... The ~200 bp indexed amplicons were purified by size selection (2% agarose, E-Gel SureSelect II, ThermoFisher), quantified (QuBit ...
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bioRxiv - Cancer Biology 2020Quote: ... Integrity of gDNA was analyzed with a 1 % E-Gel™ EX Agarose Gel (Thermo Fisher Scientific). To obtain DNA from formalin-fixed ...
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bioRxiv - Microbiology 2020Quote: The purified JEV structural proteins (C, M, E, ED III) from the S2 insect expression system (Invitrogen) were quantified by using the bicinchoninic acid (BCA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the upper channel of the Liver-Chip was maintained in William’s E Medium (WEM) containing Glutamax (Gibco), ITS+ (Corning) ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were run either on pre-cast E-PAGE 8% 48-well gels (ThermoFisher Scientific, CAT# EP04808) or in hand-poured 8% SDS-PAGE gels ...
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bioRxiv - Cancer Biology 2020Quote: ... and imaged using an E-Gel Imager System with Blue Light Base (Life Technologies, Carlsblad, CA, USA).
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bioRxiv - Immunology 2019Quote: ... Cells were stained using an anti-E antibody (4G2; ATCC) conjugated to Alexa Fluor 647 (ThermoFisher; A20186) at 1:400 for 30min ...
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bioRxiv - Microbiology 2020Quote: ... site-directed mutagenesis of prM/E in a pHLsec vector51 was performed using Pfx DNA polymerase (Invitrogen). The pHLsec-prM/E wild-type and point mutants were then further amplified via polymerase chain reaction (PCR) ...
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bioRxiv - Immunology 2020Quote: ... Frozen tissue blocks were then sectioned at 10 μm on a Shandon Cryotome E (Thermo Fisher Scientific). Sections were stored at −80°C until use.
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bioRxiv - Cancer Biology 2022Quote: ... were added to each field on the slide before adding coverslips (Fisher Scientific, Cat. # 12-544-E). For the second method ...
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bioRxiv - Bioengineering 2022Quote: ... pBAE:miR-29b complexes were freshly prepared and loaded in 2% E-Gel Precast Agarose Gels (Thermo Fisher), run following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The quality of gene expression libraries is confirmed using 2% E-Gel (ThermoFisher Scientific, Waltham, MA, USA). We quantified the sequencing libraries using Qubit (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... and the synthesized HLA-E gene were digested separately using HindIII and EcoRI enzymes (ThermoFisher, Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of PCR products was run on a 2% E-gel EX Agarose Gel (ThermoFisher #G401002) for 10 minutes according to the settings programmed into the gel system ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples loaded onto EX Agarose Gels were mixed with the E-Gel sample buffer (1X) (Thermo Scientific). Agarose gels were visualized under blue light using a Fas-DIGI illuminator (Geneflow) ...
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bioRxiv - Pathology 2024Quote: ... assembled and filled with Williams Medium E glutamax-I buffer (WME-solution Thermo Fisher, Waltham, Massachusetts, USA) with 25 mM D-glucose (Merck ...
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bioRxiv - Immunology 2023Quote: ... rat monoclonal anti-MHC class II (I-A/I-E) (1:250, M5.114.15.2, Invitrogen #14-5321-82), and following secondary antibodies were used ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
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bioRxiv - Developmental Biology 2023Quote: ... Some amplified libraries were size selected for 300 - 800 bp on a 2% EX E-gel (Thermofisher) and purified using QIAquick gel extraction kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were stained with either hematoxylin and eosin using Abcam H&E Staining Kit (Fisher Scientific, NC1881153) or Luxol Fast Blue (LFB ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μm sections were prepared with the Epredia HM 340 E electronic rotation microtome (Thermo Fisher Scientific) and transferred onto Superfrost Plus Slides (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with hematoxylin and eosin (H&E) and coverslip using Permount (Fisher Scientific, Pittsburgh, PA).
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bioRxiv - Neuroscience 2022Quote: ... cortices of embryonic day (E) 18/19 Wistar rats were dissociated in trypsin with 0.25% EDTA (Gibco), washed after 20 min of digestion in plating medium (see below) ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were added to 2% E-Gel agarose gels with SYBR Safe DNA Stain (Invitrogen) and run in an E-Gel PowerSnap system (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: Purified GCTM-5+ iLPCs were expanded in LPC medium comprising base medium of Williams’ Medium E (ThermoFisher), with 10% foetal calf serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from the ventricles of embryonic day (E) 16.5 hearts were isolated using TRIzol (Life Technologies) as described ...
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bioRxiv - Genomics 2023Quote: ... the cassette was loaded onto the E-Gel Power Snap Electrophoresis Device (Thermo Fisher Scientific, Cat # G8100) and run for 13 minutes on the 1-2% agarose gel setting ...
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bioRxiv - Immunology 2023Quote: U251 cell variants (U251 WT and U251 GspHLA-E) were labeled with two different CellTrace Violet (ThermoFisher) concentrations (0.1 μM ...
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bioRxiv - Biochemistry 2024Quote: ... and inorganic pyrophosphatase (PPase) were added according to the EnzCheck pyrophosphate assay kit (ThermoFisher, Cat # E-6645). The samples were briefly centrifuged for 3 min at 10,000 x g to remove any precipitated Sg MT ...
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bioRxiv - Biophysics 2019Quote: ... N-terminal-tagged MBP-TRPA1 under the control of a CMV promoter was expressed in HEK293F cells using the BacMam system (Thermo Fisher). Protein expression was induced at cell density 1.5-2.5 × 106 cells ml- with media supplemented with 5 mM sodium butyrate and 5 µM ruthenium red ...
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bioRxiv - Developmental Biology 2021Quote: ... HA and Myc tagged proteins were visualized on an Amersham Hyperfilm MP (28906845, Cytiva) using SuperSignal WestDuo (34075, Thermo Fisher Scientific).
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bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3x in PBS with 2% FBS and incubated with secondary antibody (Alexa Fluor 488-conjugated goat anti-mouse IgG) and fluorescently-tagged phalloidin (Molecular Probes), in a humidity chamber for 1hr ...