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Citations for Thermo Fisher :
3901 - 3950 of 5129 citations for JIP3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... reduced and alkylated followed by overnight trypsin digestion and peptide extraction following manufacturer’s instructions (In-Gel Tryptic Digestion kit, ThermoFisher Scientific). Extracted peptides were vacuum dried and redissolved in 2% acetonitrile in 0.1% formic acid in water.
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bioRxiv - Systems Biology 2024Quote: ... Peptides was were loaded via an Acclaim PepMap 100 trap column (C18, 5 μm, 100Å; Thermo Scientific, San Jose, CA) onto a Waters BEH C18 column (100 µm × 100 mm ...
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bioRxiv - Microbiology 2024Quote: Peptide mixtures were analyzed using two-dimensional (2D) liquid chromatography (LC) on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) coupled with a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Sorted lung DC1 were loaded with 10 nM MHC class I-restricted OVA peptide (OVA257-264, SIINFEKL) in complete IMDM media (Gibco) for 1.5 hours at 37°C in a Eppendorf tube ...
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bioRxiv - Developmental Biology 2024Quote: Peptides were detected by tandem mass spectrometry using a Q Exactive Plus hybrid quadrupole-orbitrap mass spectrometer (Thermo Fisher Scientific). The Nanospray Flex ion source (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coupled to an UltiMate 3000 HPLC system for on-line liquid chromatographic separation .Tryptic peptides for each sample were loaded onto a C18 trap column (ThermoFisher Scientific Acclaim PepMap 100 ...
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bioRxiv - Molecular Biology 2024Quote: ... digested peptides were acidified with 0.8% TFA (final) and analyzed by LC– MS/MS on an EASY-nLC 1000 (Thermo Scientific) with a two-column setup ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were applied on an Acclaim PepMap 100 C18 trap column (75 µm ID × 2 cm, 3 µm; Thermo Scientific) in 0.1% formic acid and 2% acetonitrile in H2O at a constant pressure of 80 MPa and separated by a linear gradient of 2%–6% buffer B in buffer A for 3 min ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides was dissolved in buffer A (0.1% Formic acid) loading into a a reverse phase trap column (Thermo Scientific Acclaim), the column was connected to a C18-reversed phase analytical column (Thermo Scientific Easy Column) ...
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bioRxiv - Microbiology 2024Quote: Digested peptides were analyzed with 90-minute gradients in C18 nanoflow reversed-phase HPLC (Dionex Ultimate 3000,3500 RSLC nano, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive Orbitrap HF ...
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bioRxiv - Cancer Biology 2024Quote: ... the resin underwent trypsin digestion and peptides were run on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) at the UNMC Proteomics Core and analyzed using Proteome Discoverer (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Coding regions of IPI-O1 and IPI-O1nlswithout a signal peptide and StCWC15a/b were amplified with primers containing attB tails and BP-cloned into pDONR207 (Invitrogen). To generate BiFC constructs ...
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bioRxiv - Genetics 2024Quote: ... Sample preparation and labeling of the peptides with TMT isobaric mass tags was performed as manufacturer’s instructions (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2024Quote: ... 25,000 rested T cells were co-cultured with DC pulsed with 10 ug/ml cognate peptides or treated with PHA as a positive control (1:100, ThermoFisher). After 5 days ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were loaded with autosampler and trapped in an Acclaim PepMap 100 trap column (75µm ξ 2 cm, Thermo Scientific) at 4 µL/min with 100% solvent A (0.1% formic acid in water ...
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bioRxiv - Cell Biology 2023Quote: LC-MS/MS analyses of peptide mixtures coming from in-gel digestions were done using RSLCnano system (Thermo Fisher Scientific) on-line connected to Impact II Qq-Time-Of-Flight mass spectrometer (Bruker ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a µPrecolumn C18 PepMap100 (5 μm, 100 Å, 300 μm×5 mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Biophysics 2024Quote: ... Eluting peptides were analyzed in data-dependent acquisition mode on an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific, Dreieich, Germany) coupled to the nano-HPLC by a Nano Flex ESI source ...
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bioRxiv - Biophysics 2024Quote: ... Eluting peptides were analyzed in data-dependent acquisition mode on an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific, Dreieich, Germany) that is connected to the nano-HPLC by a Nano Flex ESI source ...
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bioRxiv - Cell Biology 2024Quote: ... and phospho-peptides were subjected to LC-MS/MS analysis using an Orbitrap QE LC-MS/MS system (Thermo Scientific) at the proteomics core facility of UT Southwestern Medical Center ...
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bioRxiv - Cancer Biology 2024Quote: Peptides were reconstituted with 2% formic acid and analyzed in triplicates on an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) coupled to an UltiMate 3000 UHPLC system (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The peptide extracts are reduced in volume by vacuum centrifugation and a small portion of the extract is used for fluorometric peptide quantification (ThermoFisher). One microgram of sample based on the fluorometric peptide assay was loaded for each LC/MS/MS analysis.
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bioRxiv - Cell Biology 2024Quote: ... Phosphopeptide fractions as well as the flow-through fraction were desalted with PierceTM Peptide Desalting Spin Columns (Thermo Fisher Scientific) according to manufacturer’s instruction and then completely dried under speed-vacuum.
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bioRxiv - Cell Biology 2024Quote: The peptides and proteins were identified by using the software Proteome discoverer v2.5 (Thermo Fisher Scientific, San José, CA, USA) with UniProtKB/Swiss-Prot ...
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bioRxiv - Bioengineering 2024Quote: ... quenchbodies were eluted from the beads using 50 μL of 1.5 mg/mL Pierce™ 3 x DYKDDDDK Peptide (ThermoFisher).
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bioRxiv - Biochemistry 2024Quote: ... Peptide mixtures of three independent replicates per protein complex were analyzed on an LTQ-Orbitrap XL (Thermo Scientific, Bremen, Germany), which was directly coupled to an UltiMateTM 3000 HPLC or RSLCnano system (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides eluted from the column were analyzed using a Q Exactive HF-X or Orbitrap Exploris 480 (Thermo Fisher Scientific) for overlapping window DIA-MS (20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Both peptide mixtures were separately analysed on LC-MS/MS system (RSLCnano connected to Orbitrap Exploris 480; Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... Conjugates were purified from excess of free antigenic peptides by several dialysis steps at 4 °C against PBS using Slide-A-Lyzer 20,000 MWCO dialysis cassettes (Thermo Scientific). STxB(70C)-SL8 and STxB(70C)-G15F conjugate formation and the absence of remaining free SL8 or G15F after purification were validated by UPLC-MS.
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bioRxiv - Neuroscience 2023Quote: ... An equal amount of each peptide sample was incubated with individual TMT plex reagents according to the manufacturer’s instructions (ThermoFisher Scientific). After incubating for 60 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: Peptides for the qualitative analysis were separated by reversed phase LC on an RSLCnano system (Ultimate 3000, Thermo Fisher Scientific) coupled online to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were analysed by nanoflow-LC-MS/MS using a Orbitrap Q-Exactive-HF™ Mass Spectrometer (Thermo Scientific TM) coupled to a Dionex™ Ultimate™ 3000 ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated on a capillary column (0.075 mm × 500 mm, Acclaim Pepmap 100, reverse phase C18, NanoViper, ThermoFisher Scientific) according to a 2-40% buffer B gradient (0.1 % formic acid ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Each sample containing a 100 μg peptide mixture was labeled using tandem mass tag reagent according to the manufacturer’s instructions (Thermo Scientific). The negative control group samples were labeled C1 ...
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bioRxiv - Molecular Biology 2022Quote: ... spectra were subjected to peptide and protein identification using the MS Amanda search engine triggered by proteome discover (version 1.4., Thermo Scientific). Here ...
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bioRxiv - Biochemistry 2023Quote: LC-MS/MS analysis of TMT-labeled peptides was conducted on an Orbitrap Fusion Lumos tribrid mass spectrometer (Thermo Scientific) coupled with an Easy-nLC 1200 UPLC system (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: The raw LC-MS/MS data was analyzed against the Uniprot GaHV2 database (taxon 10390; 1300 sequences) using the Byonic peptide search algorithm (Protein Metrics) integrated into Proteome Discoverer 2.4 (Thermo Scientific). Optimal main search settings were initially determined with Byonic Preview (Protein Metrics ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated on a PepmapTM C18 reversed phase column (3 μm, 100 Å, 75 μm × 50 cm) (Thermo Scientific). Samples were fractionated with mobile phase A consisting of 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted and ionized by electrospray followed by mass spectrometric analysis on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). MS data were acquired using the FTMS analyzer and MS/MS data using the ion trap analyzer ...
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bioRxiv - Microbiology 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Fisher Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged proteins were then eluted with room temperature Elution Buffer with 2.5 mg/mL 3XFLAG Peptide (Thermo Fisher # A36806) for 15 min at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Each peptide extract was loaded on a 300 µm ID x 5-mm PepMap C18 pre-column (Thermo Scientific, USA) at a flow rate of 10 µL/min ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted and separated on an C18 Acclaim PepMap RSLC analytical column (75 µm x 250mm, Thermo Fisher Scientific) with a flow rate of 300 nl/min in a 120 min gradient of 3% buffer A (0.1% formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 200 ng peptide material was measured on a Q Exactive HF-X Orbitrap mass spectrometer (Thermo Fisher), coupled to the LC-system with a nano-spray ion-source using coated emitter tips (PepSep ...
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bioRxiv - Biochemistry 2023Quote: ... The digestion was quenched in 0.5% formic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/µL ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed and then treated with peptide in NP-40 and Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoFisher) for 10 minutes at room temperature while rocking ...
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bioRxiv - Genetics 2023Quote: ... Bin3-3xHA complexes were purified by two sequential competitive elutions with 75 µl of Pierce HA Peptide (Thermo Scientific, 26184) diluted to 2 mg/ml in Nuclease-Free Water (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were analyzed using a 140 min water-acetonitrile gradient delivered by a Dionex Ultimate 3000 UHPLC (Thermo Fisher Scientific) operated initially at 400 nL/min flow rate with 1% buffer B (acetonitrile solution with 3% DMSO and 0.1% formic acid ...
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bioRxiv - Plant Biology 2023Quote: Peptide/Protein identification and MS1-based label-free quantification were carried out using Proteome Discoverer 2.5 (PD2.5) (Thermo Fisher Scientific) or Skyline version 22.2 (MacCoss lab software) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteolytic peptides were finally concentrated in a trapping column (C18 Pepmap, 300 μm i.d. × 5 mm length, Thermo Fisher Scientific).