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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: Drosophila phospholipids and diacylglycerols were analyzed by direct-infusion into an electrospray ionization triple quadrupole mass spectrometer (Applied Biosystems 4000 Q-trap mass spectrometer ...
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bioRxiv - Microbiology 2024Quote: ... Genotype of mice was confirmed with PCR where DNA was extracted from mouse tissue using Direct PCR (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... the Q Exactive plus was operated in a data-dependent mode and under direct control of the Thermo Excalibur 3.1.66 (Thermo Scientific). The MS data were acquired using the following parameters ...
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bioRxiv - Synthetic Biology 2021Quote: All the agar plates were prepared by either Universal Plate Pourer (KREO Technologies Inc.) or manually in Nunc™ OmniTray™ Single-Well Plate (ThermoFisher Scientific 264728). To minimize noise of colony size ...
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bioRxiv - Immunology 2021Quote: ... and 40 μL of this mixture was added to the plates in a 1:1 ratio (Thermo-Fisher Scientific, catalog nos. 10008D and 10009D) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Sorted LSK cells were cultured in 48-well-plates for 48 hours in a 1:1 ratio of Stem Pro-34 SFM (Gibco/ Thermo Scientific, Waltham, MA, USA) and Iscoves modified Dulbecco medium (IMDM ...
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bioRxiv - Microbiology 2022Quote: ... Antibiotics were then added to each bacterial suspension at a 1:1 ratio (100 uL bacteria:100 uL antibiotic) in a 96-well plate (Fisher Scientific, #07-000-108) resulting in a final concentration of 5×105 CFU/mL of bacteria and antibiotics ranging from 0.03125-1400 ug/mL ...
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bioRxiv - Cell Biology 2020Quote: The frozen supernatants were used for IL-2 ELISAs according to the manufacturer’s protocol (KMC0021, Thermofisher).
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bioRxiv - Cancer Biology 2019Quote: ... the concentration of Interferon-γ (IFNγ) in the media was measured by ELISA (Invitrogen, 88-7314). The T cells from 24-hour co-cultures were harvested ...
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bioRxiv - Immunology 2021Quote: ... The colorimetric reaction was terminated by the addition of 100 μL of ELISA Stop Solution (Invitrogen). The absorbance at 450 nm and 650 nm was read by spectrophotometry using a SpectraMax iD3 microplate reader (Molecular Devices).
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bioRxiv - Immunology 2019Quote: ... the concentration of IFN-γ in the cell culture supernatant was determined by ELISA (Invitrogen, #EHIFNG2). An IFN-γ standard was included in the ELISA and linear curve fitting using the GraphPad Prism 7.0 a software was used to derive the IFN-γ concentration in the samples from the absorbance at 450 nm (A450 ...
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bioRxiv - Cancer Biology 2020Quote: ... O.D values were determined at a wavelength of 590 nm using an ELISA reader (Thermo Scientific) The percentage survival was determined as the ratio of treated cells versus vehicle control after background subtraction ...
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bioRxiv - Biochemistry 2021Quote: ... Background binding level in ELISA assays was measured using Human IgG Isotype Control (ThermoFisher #02-7102).
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bioRxiv - Neuroscience 2020Quote: ... Pierce HRP ECL substrate was used to detect luminescence in ELISA (Thermo Fisher Scientific, USA, #32209). Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA (Thermo Fisher Scientific #88-8086-77). Alternatively ...
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bioRxiv - Microbiology 2022Quote: ... followed by subsequent addition of Turbo 3,3′,5,5′ tetramethylbenzidine (TMB)-ELISA substrate solution (Thermo Fisher Scientific) and 2M sulfuric acid stop solution ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were used to perform ELISA to quantify the levels of IL-1β (Thermo Fisher Scientific), IL-6 (Biolegend) ...
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bioRxiv - Biochemistry 2024Quote: ... Signal was developed by treating cells with 200 μl TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue colour appeared ...
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bioRxiv - Immunology 2022Quote: ... TNF concentrations in the supernatants were measured by ELISA (Fisher Scientific, Waltham, MA, Cat# 88-7346).
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were harvested for IL-1β measurement using a specific ELISA (Thermo Fisher, 88-7621-22). Apoptotic cells were generated by retroviral transduction or MTX treatment during 20-24 h when cell death approaches its maximum ...
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bioRxiv - Pathology 2024Quote: ... The total amount of TGFβ1 was determined on technical duplicates by ELISA (88-8350-22, Invitrogen), after heat denaturation followed by acidic treatment and neutralization according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance was measured at a wavelength of 450 nm using an ELISA microplate reader (Thermo Fisher). Assays were repeated at least three times.
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bioRxiv - Immunology 2023Quote: ... supernatant was collected and IFN-ɑ measured by ELISA according to manufacturer’s instructions (Invitrogen; catalog # BMS6027)
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bioRxiv - Neuroscience 2024Quote: ... Levels of cerebral Aβ40/42 were determined by ELISA according to the manufacturer’s instructions (Invitrogen, CA). Furthermore ...
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bioRxiv - Immunology 2024Quote: ... IL-5 levels were quantified in undiluted BAL fluid by ELISA following the manufacturer’s instructions (Invitrogen).
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bioRxiv - Bioengineering 2024Quote: ... Cystatin A levels were determined by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (Invitrogen) and calculated using standard curve interpolation ...
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bioRxiv - Biochemistry 2024Quote: ... was measured by ELISA of secreted mIFN-γ in cell supernatants (Invitrogen REF 88-8314-22).
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bioRxiv - Cell Biology 2023Quote: Mitochondrial membrane potential of the sperm samples was assessed with the JC-1 fluorescent probe using the kit “MitoProbe™ JC-1 Assay Kit for Flow Cytometry (M34152, Invitrogen by Thermo Fisher Scientific). Diluted sperm in SFMM was incubated for 15 min at 20 °C with JC-1 at a final concentration of 0.5 µM ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibodies were added for 30 min at room temperature and the membranes were washed 3x 5 min in TBST again and developed with ECL (SuperSignal™ West Pico PLUS Chemiluminescent Substrate, Thermo Scientific, cat#34580 or SuperSignal™ West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were analyzed by SDS PAGE and Western blot using HRP-labeled secondary antibodies and SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Films with representative exposure times were scanned ...
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bioRxiv - Cell Biology 2020Quote: ... then incubated with appropriate primary antibodies and HRP or near-IR-conjugated secondary antibodies and visualized using chemiluminescence (SuperSignal West Femto Chemiluminescent Substrate; Thermo Fisher Scientific), or a Li-Cor Odyssey fluorescence scanner ...
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bioRxiv - Neuroscience 2019Quote: ... Target protein bands detection was done in Gel Documentation system (Syngene: chemiXX9) using Super signal West Pico Chemiluminescent substrate (Thermo Scientific, #34077) and densitometric quantitation assessed using Image Studio Lite v5.2 (LI-COR Biosciences).
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bioRxiv - Cancer Biology 2019Quote: ... The membranes were then washed with TBS-T buffer followed by exposure to the appropriate horseradish peroxidase-conjugated secondary antibody for 1h and visualized on either Kodak X-ray film (GeneMate, F-9023-8×10) or BioRad ChemiDoc Imaging System using either SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, 34080) or ECL Prime Western Blotting Detection Reagent (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... samples were UV cross-linked and the biotin signal detected with HRP–conjugated streptavidin using the Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... and visualized using SuperSignal West Pico PLUS Chemiluminescent Substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Biochemistry 2021Quote: ... The blot was washed three times (15 mL PBS-T, 10 min, rt) and WGA was visualized using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific).
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bioRxiv - Biochemistry 2022Quote: ... Immunoblots were visualized by film exposure using horseradish peroxidase-conjugated secondary antibodies and SuperSignal West Femto (DNMT3A) or SuperSignal West Pico PLUS (other proteins) chemiluminescent substrates (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Proteins were quantified with Image Studio Version 5.2.4 (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was washed two times with TBS-T and one time with TBS (TBS-T without Tween-20) before SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific 34077) was used for imaging ...
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bioRxiv - Plant Biology 2022Quote: ... Biotinylated proteins or Myc-tagged miniTurbo were visualized on the membranes using a luminol-based chemiluminescent substrate that is oxidized by HRP in the presence of peroxide (Thermo Fisher; 34577). The membranes were stained with Coomassie staining solution (60 mg l-1 Coomassie brilliant blue ...
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bioRxiv - Microbiology 2022Quote: ... secondary antibodies (Kirkegaard & Perry Laboratories, Gaithersburg, MD) were used and visualized by SuperSignal West Dura chemiluminescent substrate or ECL (Thermo Fisher Scientific). All Western blots were performed with at least three biological and technical replicates and significance determined by t-test analysis.
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bioRxiv - Biophysics 2022Quote: ... Membranes were then washed three times before being subjected to SuperSignal West Pico PLUS Chemiluminescent Substrate or Dura Extended Duration Substrate (Thermo Fisher Scientific) peroxide solution treatment for visualization of antibody-bound proteins using a Bio-Rad ChemiDoc Touch Imaging System.
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bioRxiv - Immunology 2020Quote: ... the membrane was washed three times for 10 min in TBST and then treated with enhanced chemiluminescent substrate (Pierce™ ECL Western Blotting Substrate, ThermoFisher Scientific). Both gels and western blots were visualized using a ChemiDoc MP Imaging system (Biorad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then washed three times with 1x TBST for ten minutes per wash and signals were measured using an ultra-sensitive enhanced chemiluminescent (Thermofisher Cat#34096) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane stained with Clean-Blot™ IP detection reagent was treated with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher 34577) for at least 5 min at room temperature before imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... signals were detected using ImageQuant LAS400 (General Electric) and images were captured using a SuperSignal™ West Pico Plus Chemiluminescent Substrate (Thermo Scientific) or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... The signal was detected by ECL using the SuperSignal West Pico Chemiluminescent Substrate or with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher scientific) and detected with a BioRad ChemiDoc™ Xrs+ ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of proteins was performed using secondary antibodies conjugated to horseradish peroxidase-HRP and the SuperSignal West Pico Plus chemiluminescent substrate (Thermo Scientific-Pierce).
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bioRxiv - Neuroscience 2021Quote: ... The membranes were washed five times with TBST and immunoreactivity was detected using SuperSignal West Pico PLUS Chemiluminescent substrate (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2020Quote: ... The membrane was washed for two minutes five times and incubated with chemiluminescent subtrate (SuperSignal™ WEST pico PLUS, Thermo Fisher Scientific) and imaged using a G:Box (Syngene) ...