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Citations for Thermo Fisher :
3901 - 3950 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... 1.5 µg of total RNA was reverse-transcribed using Superscript II (Life Technologies). Relative expression levels of genes of interest were determined by real-time RT-PCR using the Absolute SYBR Green ROX Mix (ABgene ...
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bioRxiv - Genetics 2024Quote: AC16 cells were grown on Nunc Lab-Tek II Chamber slides (ThermoFisher, 154461). Cells were fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Immunology 2024Quote: ... reverse transcription was done with SuperScript™ II reverse transcriptase (Invitrogen, Catalog: 18064014), oligo-dT30VN (1 µM ...
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bioRxiv - Biophysics 2024Quote: ... SF9 insect cells were cultured in Sf-900™ II SFM medium (Gibco) supplemented with 1 x GlutaMax (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA synthesis was performed with 1ug RNA and SuperScript II reverse transcriptase (Invitrogen) following manufacturer’s instructions and was diluted 1:20 in water before qPCR analysis ...
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bioRxiv - Genetics 2024Quote: ... which were converted into cDNA with Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) according to manufacturer’s instruction ...
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bioRxiv - Systems Biology 2024Quote: ... RNAs were then subjected to reverse transcription using SuperScript II (Life Technologies, # 18064014) or high-capacity cDNA reverse transcription kits (Applied Biosystems ...
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bioRxiv - Systems Biology 2024Quote: ... with 4mg/mL AlbuMAX™ II Lipid-Rich Bovine Serum Albumin (GIBCO™), 1× MACS NeuroBrew-21 with Vitamin A (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by counting in chamber slide using Countess II Automated Cell Counter (Invitrogen). Resuspension buffer was added to the nuclei suspension to achieve a final concentration of 700-1200 nuclei/µl ...
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bioRxiv - Cell Biology 2024Quote: ... were transfected with the bacmid DNAs using Cellfectin II (10362100; Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... and cDNA was made using a Superscript II reverse transcriptase reaction (Invitrogen, UK). cDNA was converted to dsDNA and cleaned up using Beckman Coulter’s RNAClean XP (1.8 ratio ...
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bioRxiv - Neuroscience 2024Quote: ... about 200 ng of RNA was reverse-transcribed using Superscript II (Life Technologies). SYBR Green PCR Master Mix (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... via LR clonase (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen # 11791020). Lentiviruses expressing NLRP1 (or vector control ...
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bioRxiv - Neuroscience 2024Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). A Sequant ZIC-pHILIC column (2.1 mm i.d ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei concentrations were determined using a Countess™ II Automated Cell Counter (ThermoFisher) and nuclei quality was assessed at 10X and 40X magnification using an Evos XL Core Cell Imager (ThermoFisher).
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bioRxiv - Neuroscience 2024Quote: ... First strand synthesis mix (1X SuperScript II first strand synthesis buffer, Thermo Fisher; 5 mM DTT ...
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bioRxiv - Plant Biology 2024Quote: ... The amplicon was recombined into pDONR207 using Gateway BP Clonase II (Thermofisher Scientific), then recombined into plasmid pK7RWG2 (Karimi et al. ...
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bioRxiv - Cell Biology 2024Quote: ... and the destination vector pDestTol2CG2 were recombined using LR Clonase II Plus (Invitrogen). To make the pDestTol2CmC2 TRE:GSP-il1βmat construct ...
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bioRxiv - Molecular Biology 2024Quote: ... Spodoptera frugiperda Sf9 cells were cultured in Sf-900 II SFM medium (Gibco) at 27°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 ml of Sf9 cells cultured in suspension in SF900-II SFM (Invitrogen) at 1.5-2 x 106 cells/ml were infected with 1.5 ml of transfection supernatant ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were counted using an automated cell counter (Invitrogen Countess® II FL) using a 1:1 ratio of cells and trypan blue (Gibco ...
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bioRxiv - Genomics 2024Quote: ... and 0.5 uL of 200 U/uL SuperScript II Reverse Transcriptase (18064014, Invitrogen). The reaction was then incubated at 25°C for 10 minutes and 42°C for 1 hour.
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bioRxiv - Immunology 2024Quote: ... Cells were stimulated with CHO-SFM II macrophage media (Gibco, Waltham, MA, USA) or 1 mg/mL OxLDL ...
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bioRxiv - Immunology 2024Quote: We coated 4 well Lab-Tek II chamber coverglass slides (Thermo Fisher Scientific) with poly-l-lysine for 1 hour at 37°C prior to seeding cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... anti-mouse-MHC II-eFluor™450 (48-5321-82, Invitrogen, 1:100), anti-mouse-CD24-SuperBright 600 (63-0242-82 ...
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bioRxiv - Genomics 2024Quote: ... in RPMI 1640 media (Fisher) supplemented with hypoxanthine and 0.5% Albumax II (Invitrogen) in 2% hematocrit ...
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bioRxiv - Cancer Biology 2024Quote: ... the minced tissues were digested with 5mg/mL collagenase Type II (Gibco, 17101015) for 1 hour at 37°C followed by TryLE (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA PCR was performed using Platinum II Hotstart Mastermix (Thermo Fisher Scientific) and edited allele frequency was detected by Sanger sequencing and analyzed by ICE57 (Table S1-2) ...
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bioRxiv - Cell Biology 2024Quote: ... complementary DNA (cDNA) was generated by using a SuperScript II RT kit (Invitrogen), as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Gateway LR Clonase II enzyme mix (cat. no. 12535-029, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured on a LabTek II chambered coverglass (#155409, Thermo Fisher Scientific) at least two hours before time-lapse imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). The mobile phase consisted of buffer A ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on Lab-Tek™ II Chamber Slide (Thermo Fisher Scientific) coated with Retronectin (Clonetech) ...
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bioRxiv - Cancer Biology 2024Quote: ... via an Ion Max ion source with a HESI II probe (Thermo Scientific). The mobile phase consisted of buffer A ...
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bioRxiv - Cancer Biology 2024Quote: ... cell plated in Nunc Lab-Tek II chamber slides (Thermo Fisher, Cat#154453) were fixed for 15 min at room temperature in 4% paraformaldehyde and then washed three times with PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... The fragment was cloned into a pCR-Blunt II TOPO vector (Invitrogen K280002) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Ligated RNAs were then converted to cDNA using Superscript II (Invitrogen, Waltman, MA). To control for differences in the amount of starting material ...
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bioRxiv - Genetics 2024Quote: ... cDNA pools were generated using SuperScript II Reverse Transcriptase (Invitrogen Cat# 11904-018). qPCR was performed with LUNA qPCR MasterMix (NEB Cat# M3003 ...
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bioRxiv - Genetics 2024Quote: ... The supernatant was removed and 5ml of Digestion Solution II [Accutase (Gibco, A1110501) supplemented with 1 mg/ml DNase I at 1:1000 at room temperature)] was added to the tubules followed by incubation with end-over-end rotation for 10 mins at RT with gentle pipetting after 5 mins to disperse the tubules ...
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bioRxiv - Cell Biology 2020Quote: The calibration was verified using a phase retrieval algorithm [20] in combination with through-focus scans of 175 nm green fluorescent beads (PS-speck, ThermoFisher, P7220, dilution 1:500) mounted in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and C18 reversed-phase nano RSLC columns (Acclaim PepMap™, 25 cm x 75 μm, 2 μm particle size, 100 Å pore size; Thermo Fisher Scientific) and operated at a temperature of 40°C ...
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bioRxiv - Genomics 2021Quote: ... Normalization of the data was achieved by quantitating the cycle time at an arbitrary fluorescence intensity in the linear exponential phase using Step One Plus Real-Time system Software (Applied Biosystems Japan, Tokyo, Japan) by calculating the ratio of the relative concentration of each mRNA relative to that of average of GAPDH and ß-actin or ß-actin only ...
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bioRxiv - Bioengineering 2021Quote: ... Isoproterenol responsiveness was read out after a 2 h feeding phase of on-chip adipocytes with the fluorescently labeled fatty acid (BODIPY™ 500/510 C1, C12; D3823, Thermo Fisher Scientific Inc.) by analyzing the release of fatty acids from the adipocytes ...
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bioRxiv - Bioengineering 2021Quote: The qualitative viability of spheroids formed in 600 μm micromolds or in micromolds of the periapical lesion and at the exponential growth phase was evaluated using the Live/Dead™ Viability/Cytotoxicity Kit (Invitrogen-Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were digested with both trypsin and chymotrypsin and the resultant peptides were loaded onto a reverse phase C18 trap column (Acclaim PepMap 100, 75 μm x 2 cm, nano viper, C18, 3 μm, 100 Å, ThermoFisher, Waltham, MA, U.S.A) using an Ultimate 3000 and washed with 50 μL of solvent A at 10 μl/min ...
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bioRxiv - Systems Biology 2022Quote: Each of the digested samples was separated by nano reverse phase C18 (RP-C18) chromatography on an Easy-nLC 1000 system (Thermo Scientific, San Jose, CA) using an Acclaim C18 PepMap®100 column (75 μm x 2 cm ...
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bioRxiv - Plant Biology 2021Quote: A small fraction of all samples were pooled and fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Pierce, Thermo Fisher Scientific, San Jose). Briefly ...
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bioRxiv - Immunology 2020Quote: Analysis of IgG Fc-glycosylation was performed with nanoLC reverse phase (RP)-electrospray (ESI)-MS on an Ultimate 3000 RSLCnano system (Dionex/Thermo Scientific, Breda, The Netherlands) coupled to an amaZon speed ion trap MS (Bruker Daltonics ...
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bioRxiv - Cell Biology 2021Quote: ... The organic phase of each cell-culture sample was normalized by total soluble protein amounts and measured by BCA assay (Thermo Scientific, 23225, Waltham, MA), whereas tissue samples were normalized according to dry weight measurements ...
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bioRxiv - Immunology 2022Quote: ... neutrophil-containing top phase was transferred into a fresh tube and washed with basal RPMI 1640 without phenol red (Thermo Fisher Scientific, Waltham, USA). If required ...