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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The ScPif1-N-His protein was labeled by binding to a 6x-His Tag Monoclonal Antibody (500 nM) conjugated to a DyLight™ 550 Fluorophore (Cat. # MA1-21315-D550, ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Immunology 2022Quote: ... and cloned Pst I/Not I in a pPPI4 expression vector containing a hexahistidine (his) tag with Gibson Assembly (Thermo Fisher Scientific). All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK ...
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bioRxiv - Immunology 2022Quote: ... The libraries were sequenced on Ion Proton using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite Software v5.2.2 (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... and Esr2b (XM_020713365) cDNA was amplified by PCR and inserted into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific, Waltham, MA). Each nucleotide sequence of the 5′-flanking region of ara and arb was retrieved from the Ensembl medaka genome assembly and analyzed for potential canonical ERE-like sequences using Jaspar (version 5.0_alpha ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was used to assess the number of cycles necessary to amplify libraries to the concentration needed for sequencing by amplifying 1 uL of library with LabTAQ Green Hi Rox master mix (Labtech) and adapter-targeted primers on a StepOnePlus Real-Time PCR system (Thermofisher Applied Biosystems). Indexing PCR involved double indexing (Kircher et al ...
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bioRxiv - Cell Biology 2024Quote: ... The precipitated proteins were visualized by SDS-PAGE and Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) to determine the protein distribution in each fraction.
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bioRxiv - Plant Biology 2023Quote: The baculovirus containing either His-mCherry-HT1 or twin strep-mVenus-MPK12 was constructed using the Bac-to-Bac™ system (Thermo Scientific). Resulting baculoviruses were used to co-infect 600 ml of Sf9 insect cells in the shaker culture at the density of 2×106 cells/ml at approximate multiplicity of infection 5-10 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed once with ice-cold PBSB and incubated with 1000x dilution of mouse anti-Myc and chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Microbiology 2023Quote: The Lysobacter enzymogenes gasdermin and the Pedobacter rhizosphaerae system were both expressed under an arabinose-inducible PBAD promoter in the pBad/His A plasmid (Thermo Scientific V43001). The Lysobacter gasdermin system was previously cloned10 and the Pedobacter rhizosphaerae system was amplified from genomic DNA ordered from the DSMZ (DSM 18610 ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysate was cleared by centrifugation at 20,000 x g for 30 min at 4 °C and purified by using GE Healthcare His GraviTrap™ Kit (Cat. No. 45-002-016, Fisher Scientific). After elution ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies against ZNF524 were purified and enriched from the serum against this recombinantly expressed His-ZNF524 using the SulfoLink® Immobilization Kit for Peptides (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... were expanded and maintained in MEMα (GibcoTM 22561021) supplemented with 10% heat-inactivated FBS (HI-FBS; LabTech FB-1001/500 or Gibco 10438-026) at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: pMT-Reps-Flag and pMT-Reps-HA were generated by cloning Reps-RA isoform from FMO04419 (DGRC Stock 1612020) into pMT-V5-His vector (Invitrogen cat#V412020). pMT-Mnb-V5 was generated by cloning Mnb-RH isoform from FMO12028 (DGRC Stock 1639183 ...
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bioRxiv - Biophysics 2024Quote: ... Lysates containing Utrophin or α-actinin were loaded on a column packed with His-Pur Ni-NTA resin (ThermoFisher Scientific, Waltham, MA). The column was washed with 0.6 M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: The piggyBac piRNA target site was inserted into the middle of the V5 coding sequence in the pIB-V5/His vector (Thermo Fisher Scientific) by site-directed mutagenesis ...
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bioRxiv - Cancer Biology 2021Quote: ... Microarray was performed by the UVA DNA Sciences core using the Human Affymetrix GeneChip Array ([HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array). Gene lists were generated from microarray data corresponding to genes >1.25 LogFC and <0.75 logFC were used in downstream analyses ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Concentrations of FGF19 in human serum samples were tested using a commercial Human Fibroblast Growth Factor 19 Assay Kit (Thermo scientific, Waltham, MA, USA). The level of serum C4 was quantified by a mass spectrometry-based method developed by our group.32
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two control samples from the same pool of total human brain RNA (Ambion FirstChoice human brain reference RNA Cat#AM6050, Life Technologies Inc., Carlsbad, CA) were included with each of the five datasets ...
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bioRxiv - Synthetic Biology 2021Quote: ... supplemented with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with cells ...
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bioRxiv - Cell Biology 2020Quote: Human fibroblast cells lines were maintained in DMEM/F12 (Gibco) with 15% FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Human IL-6 and IL-8 ELISAs were from Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific, MA, USA), and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... FITC-conjugated mouse anti-human SOX2 (ThermoFisher Scientific, MA, USA), BV421-conjugated mouse anti-human CD34 (Biolegend ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney cells (HEK293) were maintained in IMDM (Gibco) supplemented with 10% FCS and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: Human PSCs resistant to neomycin were incubated with Accutase (Gibco) in 37°C for 5-10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Human staining panels included anti-CD3-Alexa 700 (Invitrogen, UCHT1), anti-CD19-APC (Biolegend ...
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bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293) T-Rex Flp-In (ThermoFisher Scientific) cells were grown in DMEM supplemented with 10% FBS in an atmosphere of 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... anti-human IgG secondary antibody labeled with Dylight 650 (Invitrogen) was applied at 1 to 1000 (∼0.5μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and 20 ng/mL human heat stable sbFGF (Gibco, PHG0367) and split using accutase (Millipore Sigma ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 2% AB human serum (Gibco; Thermo Fisher Scientific). T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2022Quote: ... supplemented with 2% AB human serum (Gibco; Thermo Fisher Scientific). T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Genomics 2020Quote: FISH probes were made from human BACs (ordered from Invitrogen, RP11-479I13 for HSPA1A locus and RP11-173P16 for HSPH1 locus) ...
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bioRxiv - Immunology 2021Quote: ... goat anti-human Ig polyvalent antibody (10 μg/ml; Invitrogen), or purified GPIIbIIIa (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... G-CSF mouse or human ProcartaPlex Simplex Kit (ThermoFisher Scientific), G-CSF ELISA kit (R&D Systems) ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: Human VIP2R (residues 24-438) was cloned into pFastBac (Invitrogen) with an N-terminal FLAG tag followed by a His8 tag ...
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bioRxiv - Immunology 2019Quote: ... labeling and hybridization to the Human GeneArray (Thermo Fisher Scientific) were performed according to the manufacturer’s protocol ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: The human neural stem cells (NSCs) were obtained from ThermoFisher/Invitrogen (N7800-100 ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... ELISA quantification of human IgG4 (#BMS2059, ThermoFisher Scientific, Waltham, MA) was carried out per manufacturer’s instruction.
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bioRxiv - Microbiology 2019Quote: ... or other components of animal or human origin (ThermoFisher, USA). HEp-2 cells were initially seeded in growth medium (DMEM medium with 5% FBS (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... Human PDA cell lines were cultured in RPMI 1640 (Gibco) containing 10% FBS ...
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bioRxiv - Physiology 2019Quote: ... and hybridization to Human GeneChip® HTA 2.0 Arrays (Affymetrix) were done as described previously (Mattiucci et al. ...
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Pluripotent Stem Cells Can Be Isolated from Human Peripheral Nerves after in vitro BMP-2 StimulationbioRxiv - Cell Biology 2019Quote: ... and 20 ng/ml human leukemia inhibitory factor (LIF, Gibco). The cells were cultured at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: Human umbilical cord stem cell derived hiPSC6.233 (Life Technologies, A18945) were grown on Matrigel-coated (MG ...
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bioRxiv - Immunology 2019Quote: ... human omental tissues were first stained with SYTOX Blue (Invitrogen) 1 in 5000 in RPMI 1640 (Sigma ...