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Citations for Thermo Fisher :
3851 - 3900 of 7824 citations for HER4 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Gibco Human Episomal iPSC clone (Control 1, Thermo Fisher Scientific) and CW30318 (Control 2 ...
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bioRxiv - Microbiology 2023Quote: ... HRP labelled goat anti-human IgE antibodies (Invitrogen, Waltham, MA) were used as the secondary antibodies for detection and the color wasa developed using TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Human female embryonic kidney HEK293FT cells (#R70007, Invitrogen; RRID: CVCL_6911), immortalized mouse embryonic fibroblasts (MEFs) ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Anti-human CD45-PerCP-Cy5.5 (45-9459-42, Affymetrix eBioscience), CD45-APC Cy7 (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... They were cultured on human recombinant vitronectin (Thermo Fisher Scientific) coated plates in StemFlex medium (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: 10 ng of fully methylated human blood (Thermo Scientific, SD1131) and Jurkat (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... A human IL-6 ELISA kit (Thermo Fisher Scientific, KHC0061) was then used as instructed by the manufacturer to measure IL-6 concentrations ...
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bioRxiv - Neuroscience 2024Quote: ... Luminex assay was performed using ProcartaPlex Human Basic Kit (Invitrogen) and a panel of ProcartaPlex Human Simplex (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and 20 ng/ml human bFGF (PHG0021, Thermo Fisher Scientific)) ...
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bioRxiv - Synthetic Biology 2024Quote: Rabbit polyclonal anti-Human ASGR1 antibody (Thermo Fisher, PA5-80356), 1:1,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant Human EGF (Invitrogen, Cat#PHG0313, 100 µg/500 mL), Hydrocortisone (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Gibco Human Astrocytes (cat. nr. K1884, Lot nr. 802268, Invitrogen) were sourced from a 20 weeks old male donor ...
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bioRxiv - Immunology 2024Quote: ... goat anti human IgG (H+L) HRP (Invitrogen, Cat#31412) was added to wells at a 1:10,000 dilution for one hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... or a human NT3 ELISA kit (Thermo Fisher Scientific, EHNTF3). Both ELISAs were performed as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed twice with human wash buffer (DMEM/F12 (Gibco), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2024Quote: ... human neutrophils were labeled with calcein AM (Thermo Fisher Scientific) and left to migrate for 2 hours before termination of the experiment ...
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bioRxiv - Bioengineering 2024Quote: ... Research-grade DynabeadsTM Human T-Expander CD3/CD28 beads (Gibco) were washed using DynaMag-2 magnet (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... CAFs were kept in Human Plasma-Like Medium (HPLM, Gibco) with ascorbic acid for 11 days ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2 Units/mL human recombinant insulin (Gibco, #12585-014), seeded on plates coated with rat tail collagen (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads Human T-Activator CD3/CD28 (11161D; Gibco; 1:100) or plate bound with anti-CD3 and anti-CD28 antibodies (0.5µg/ml each ...
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bioRxiv - Immunology 2024Quote: ... or anti-human IgG Alexa Fluor 647 conjugate (Invitrogen, A21445) or Streptavidin Alexa Fluor 647 conjugate (Invitrogen ...
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bioRxiv - Physiology 2024Quote: Human islets were cultured in CMRL medium (Gibco 11530-037) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 ng/mL recombinant human EGF (Gibco Cat# PHG0311).(22 ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs were purchased from Life Technologies (for human cell lines) and from Horizon Discovery (for human PCLS) ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human EGF (Thermo Fisher Scientific, #PHG0311, 50 ng/ml), N-acetylcysteine (Sigma Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies, Carlsbad, CA).
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bioRxiv - Molecular Biology 2021Quote: ... enrichment and chip loading was performed on an Ion Proton Chef system using the Ion PI™ Hi-Q™ Chef Kits (ThermoFisher Scientific); sequencing was performed on an Ion Proton PI™ V3 chip ...
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bioRxiv - Immunology 2022Quote: ... Human PBMCs and C57BL/6 mouse PBMCs not immediately used in any experiments were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Cell Biology 2020Quote: ... purified His-calmodulin was dialyzed in a Spectra/Por™ 4 RC Dialysis Membrane Tubing (08-667D, Thermo Fisher Scientific, Waltham, MA, USA) against 1,000 volumes of PBS supplemented with 0.5 mM EGTA at 4 °C for 4 h and replaced with fresh 1,000 volumes of PBS twice.
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Molecular Biology 2024Quote: ... were amplified from differentiated P19 mouse embryonal carcinoma cells15 and ligated into the multiple cloning site of the expression vector pcDNA3.1 myc-His B (Thermo Fisher Scientific; Waltham, MA). Snapin was cloned in-frame with the downstream myc-His tags ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Genetics 2024Quote: ... Membrane was stripped using PVDF stripping solution (DOT Scientific 65000-500) according to manufacturer instructions and reprobed with anti-6x-His primary antibody (Thermo Fisher MA1-21315) and conducted identically to the first probing ...
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bioRxiv - Neuroscience 2023Quote: An PPP2R2B-AS1 fragment containing the region 44 bp upstream of the CTG repeat and 145 bp downstream from the repeat was PCR-amplified and cloned into pcDNA3.1(-) myc-his A vector (Life Technologies, Grand Island, NY) at EcoRV site ...
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bioRxiv - Immunology 2023Quote: ... The resulting libraries were multiplexed and sequenced with Ion Proton technology and Ion Hi-Q chemistry (Ion Torrent, Thermo Fisher Scientific, Vienna, Austria). CUTADAPT 4.0 was used to trim sequencing reads of low-quality bases and poly-A tails with the options -q 20 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pull-down assay follows the protocol provided by Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA). The fluorescence intensity (excitation = 485 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
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bioRxiv - Cell Biology 2023Quote: Primary antibodies used in western blotting (WB) and immunofluorescence (IF) were anti-6x His mouse monoclonal (Invitrogen, #MA1-21315, 1:5000 in WB), anti-GST mouse monoclonal (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... to digest the phosphorylated OsPrx20-His and analyzed by the LC-MS/MS system (LTQ Orbitrap XL ETD; ThermoFisher Scientific, Waltham, MA, USA) as described previously (Gampala et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... Green fluorescent protein (GFP) standard curve was created from dilutions of Aequorea Victoria GFP His-tag recombinant protein (ThermoFisher Scientific, Cat. No. A42613) and measured on the BioTek Cytation5 Imaging Reader ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...