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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 0.4 vol glycerol (100% solution), 0.12 vol 2X dialysis buffer (100 mM HEPES-KOH at pH 7.2, 0.2 M NaCl (Invitrogen, AM9759), 0.2 mM EDTA (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested by addition of 100μl of 0.05 M ABC containing 0.3 μg of trypsin (90057, Thermo Scientific) at 37°C for 16 hours ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of total RNA was used for cDNA synthesis using the M-MLV reverse Transcriptase Kit (Invitrogen) and an oligo(dT ...
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bioRxiv - Cell Biology 2019Quote: ... To arrest cells in M phase they were treated with 1:100 KaryoMAX Colcemid solution (15212012; Gibco, UK) in PBS for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... The gonads were removed and placed in 1 M phosphate buffer saline (PBS) with 1x streptomycin penicillin (Gibco). They were then rinsed and subsequently cut into pieces of about one millimeter ...
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bioRxiv - Neuroscience 2022Quote: ... The slides were washed in 0.1 M PB and cover-slipped using Prolong Gold Antifade (Fisher Scientific P36930). Images were taken on Olympus FluoView 1200 confocal microscope or Olympus FluoView 3000 confocal microscope ...
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bioRxiv - Bioengineering 2022Quote: ... Silk was dried and subsequently dissolved in 9 M LiBr (199870025, Acros, Thermo Fisher Scientific, Breda, The Netherlands), filtered ...
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bioRxiv - Genomics 2022Quote: ... Cells were passaged by incubation in 0.5 mM EDTA-PBS (stock: 0.5 M EDTA, AM9260G, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... then incubated for 30-45 m with goat anti-mouse Alexa Fluor 555 (Invitrogen cat#A32727, 1:1000) or goat anti-rat Alexa Fluor 488 (Invitrogen cat#A11006 ...
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bioRxiv - Microbiology 2022Quote: ... First stage PCR amplification was carried out using DreamTaq Green PCR master mix (M/s Thermo scientific, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) respectively supplemented with proteinase and phosphatase inhibitors (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... before conducting immunoprecipitation based on the instructions of the M-280 Sheep anti-Rabbit IgG Dynabeads (11203D, Invitrogen). The mRNA with m6A enrichment was assayed using RT-qPCR ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 300 ng of total RNA using the M-MLV Reverse Transcriptase (ThermoFisher Scientific). SYBR® Green JumpStart™ Taq ReadyMix™ (Merck ...
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bioRxiv - Microbiology 2022Quote: ... in phosphate buffer (0.1 M, pH 7.0) before inspection using a TalosTM transmission electron microscope (TEM) (ThermoFisher, USA).
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bioRxiv - Neuroscience 2023Quote: ... Antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit or mouse IgG (Thermo Fisher Scientific) for 6h at 4°C in the following day ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed in 0.1 M PB and cover-slipped using Prolong Gold Antifade (Thermo Fisher Scientific). Images were taken using an Olympus FluoView 3000 confocal microscope and analyzed using Cellsens (Olympus ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragmented nascent RNA was purified using 30 μl of Streptavidin M-280 magnetic beads (Thermo Fisher Scientific) and ligated with 3’ RNA adapter (5’p-GAUCGUCGGACUGUAGAACUCUGAAC-/3InvdT/) ...
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bioRxiv - Systems Biology 2022Quote: ... The supernatant was separated and incubated with 10 µL of Dynabeads M-450 anti-mouse IgG (Invitrogen #11041) for 10 minutes ...
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bioRxiv - Biophysics 2022Quote: ... CD4 and CCR5 overexpressing HeLa cells (gift of David M. Rekosh, University of Virginia) were maintained in Iscove’s Modified Dulbecco’s Medium (Gibco) supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic with 0.5 mg/mL of G418 (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: Primary human fibroblasts with or without mutant mitochondrial DNA (m.3243G) were routinely maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) containing 25 mM glucose ...
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bioRxiv - Immunology 2023Quote: ... and 40 µL per mL of complex of 1 M formaldehyde prepared from 37 % stock (Thermo Fisher, 10630813). The complex was incubated for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... Annie Newell-Fugate at Texas A&M University) were cultured in Eagle’s minimal essential medium (EMEM; Fisher Scientific) using the same supplements and environments described for HFF culture ...
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bioRxiv - Cancer Biology 2024Quote: ... The biotinylated RNA-RNA complexes were pulled down by incubation with Dynabeads M-280 Streptavidin (Thermo Fisher Scientific) for 4 hr at 40C ...
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bioRxiv - Biophysics 2024Quote: ... The resulting peptide-mRNA-DNA fusions were then incubated with PfUCLH3 immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... the PBS was aspirated and cells were lysed in complete M-PER buffer (Thermo Scientific, Rockford, IL, USA). Complete M-PER buffer was prepared through addition of Protease & Phosphatase Inhibitor Cocktail (1X final ...
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bioRxiv - Evolutionary Biology 2022Quote: ... full MS/MS scans (375–1500 m/z) were performed in the Orbitrap mass analyser (Thermo Fisher Scientific) with a 60,000 resolution at 200 m/z ...
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bioRxiv - Molecular Biology 2022Quote: ... the pH was lowered to 8 by addition of 1 M Tris base and monobromobimane (mBBr, Life technologies) was added in excess to derivatize the CoASH ...
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bioRxiv - Molecular Biology 2023Quote: ... one volume of chromatin was mixed with 1/10 volume of Dynabeads M-270 Epoxy (ThermoFisher, LOT 01063817) coupled to immunoglobulin G (IgG ...
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bioRxiv - Cell Biology 2023Quote: ... A TR-FAME column (length: 30 m, inter diameter: 0.25 mm, film size: 0.25 μm, 260M142P, Thermofisher Scientific) was used with helium as carrier gas ...
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bioRxiv - Immunology 2023Quote: ... and placed into M Tubes (Miltenyi #130-093-236) with 6ml of RIPA Lysis buffer (Thermo Scientific #89900) with 1x HALT protease inhibitor cocktail (Thermo Scientific #78429) ...
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bioRxiv - Genetics 2023Quote: ... cells were washed twice with cold PBS and lysed in M-Per Mammalian Protein Extraction Reagent (Thermo Scientific) supplemented with protease inhibitors ...
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bioRxiv - Biophysics 2023Quote: ... potassium phosphate buffer (KP) 0.5 M at pH = 7 (adjusted with pH meter Orion Star A111, Thermo Fisher). BSA and urease stock solutions concentration are measured after their preparation using a UV-vis spectrophotometer (Cary 60 Spectrophotometer ...
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bioRxiv - Neuroscience 2023Quote: 45 □m Coronal slices of the raphe nuclei and hippocampus were sectioned using a cryostat (Thermo Fisher Scientific) and collected in 1x PBS ...
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bioRxiv - Immunology 2023Quote: ... Total protein from B16F10 melanoma was harvested using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) with the Complete mini EDTA-free (Roche) ...
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bioRxiv - Cell Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) and supplemented with proteinase and phosphatase inhibitors (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Immediately after thoroughly rinsing the muscle with 0.1 M phosphate-buffed saline (pH=7.4, PBS, ThermoFisher Scientific, USA), the interscutularis muscle was immersed with a solution containing 2% glutaraldehyde (Glut. ...
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bioRxiv - Immunology 2022Quote: ... The resulting monocytes were then differentiated with 50 ng/ml human M-CSF (Miltenyi) for 6 days in Iscove’s modified Dulbecco’s medium (Gibco) containing 5 % fetal calf serum (CAPRICORN ...
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bioRxiv - Genomics 2022Quote: ... and biotinylated RNA was enriched with streptavidin-coated beads (DYNAL™ Dynabeads™ M-280 Streptavidin, ThermoFisher 11205D). qRT-PCR evaluation was performed also with the primers suggested by Fuchs et al ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 µL of dissolving solution (0.1 g/ml SDS in 0.01 M HCl; Thermo Fisher, Rockfold, IL, USA) was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 μL Tris pH 6.5 1 M and 1 μL Proteinase K (20 mg/ mL, Thermo Fisher Scientific) and incubated shaking for 3 hours at 45°C ...
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bioRxiv - Immunology 2023Quote: ... cells of the cultured YUMMER1.7 cell line(31) (a gift from M. Bosenberg) were detached with 0.5% trypsin-EDTA (Gibco) for 3 minutes ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of 0.1 M CaCl2 and 0.4 μl of 100 U/μl MNase (88216, Thermo Fisher Scientific) were then added ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription reaction was performed on the same amount of total RNA using M-MLV reverse transcriptase (Invitrogen). Real-time qPCR was performed on the CFX96 Real-Time PCR Detection System using the SsoFast EvaGreen Supermix (Bio-Rad) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged by incubation in 0.5 mM EDTA-PBS (stock: 0.5 M EDTA, AM9260G, Thermo Fisher Scientific). hESCs were seeded at a density of 104 cells/cm2 in Essential 8™ medium ...
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bioRxiv - Developmental Biology 2023Quote: ... in splitless mode equipped with a TG-5MS capillary column (30 m × 0.25 mm × 0.25 μm, Thermo Scientific), using helium as carrier gas (1.0 ml/min) ...
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bioRxiv - Cell Biology 2023Quote: ... Bond-breaker TCEP solution (0.5 M) and protease inhibitor cocktail (100×, EDTA-free) were purchased from Thermo Fisher Scientific (Rockford ...
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bioRxiv - Microbiology 2023Quote: ... isolation window: 1.0 m/z) to acquire MS and MS/MS spectra by Xcalibur 4.0 (Thermo Fisher Scientific). MS/MS spectra of biogenic and synthetic molecules were acquired and directly compared ...
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bioRxiv - Molecular Biology 2023Quote: ... the PCR product was coupled to 25 μl streptavidin-coated paramagnetic beads (Dynabeads Streptavidin, M-280, Thermo Scientific) for 15 min and washed with 3 × 1000μl of PBS ...
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bioRxiv - Cell Biology 2023Quote: ... in 0.01 M PBS (sodium chloride, potassium chloride, sodium phosphate dibasic, potassium phosphate monobasic, Fisher Scientific, Pittsburgh, PA), pH 7.3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was measured spectrophotometrically (ε280 21430 M-1cm-1, MW 24.79 kDa) using NanoDrop One (Thermo Scientific). Dialysed MSP1D1 was concentrated to 5 mg/ml using a 10 kDa MWCO Amicon Ultra centrifugal concentrator (Millipore) ...