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Citations for Thermo Fisher :
3801 - 3850 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Total protein concentration was determined using the BCA Protein Assay kit (Thermo Scientific, #23225). Before LC-MS/MS analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysate concentrations were determined with Pierce Micro BCA Protein Assay kit (ThermoFisher, 23235). Equal total quantities of protein were denatured using Laemmli sample buffer supplemented with tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein concentration was measured using Bicinchoninic Acid Protein Assay Kit (Thermo Scientific, # 23227). Then ...
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bioRxiv - Cell Biology 2024Quote: ... RNA quantification was performed with Qubit RNA HS Assay Kit (catalog number: Q32852, Invitrogen). The RNA samples for RNASeq analysis were aliquoted and further processed by SBPMDI Genomic Facility ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell growth assay was performed with FluoReporter Blue Fluorometric dsDNA Quantitation Kit (Invitrogen, F2962). One 96-well plate was harvested ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein in the supernatant was quantified using the BCA protein assay kit (Thermo Scientific). Equal amounts of protein from cell lysates were resolved on a NuPAGE Bis-Tris polyacrylamide gel (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein concentration was quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific). Oil Red O extraction value was normalized to total protein in the sample ...
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bioRxiv - Cancer Biology 2024Quote: ... and EV concentration was determined using Pierce™ BCA protein assay kit (ThermoFisher Scientific) following manufacturer’s instructions and as before [34] ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined using a Pierce™ BCA Protein Assay Kit (Thermo Fisher). Lysates were boiled at 95 °C for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Extracted proteins were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific 23225). 75-100µg of protein lysates were mixed with Laemmli 6x SDS sample buffer (Boston BioProducts ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of protein was determined using the Pierce BCA protein assay kit (Thermofisher). Equal amounts of proteins were run on the 8% SDS-PAGE and were transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2024Quote: ... Protein content was measured using Pierce BCA Protein Assay Kit (23227, Thermo Fisher Scientific). Lysates were separated by SDS-PAGE under reducing conditions ...
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bioRxiv - Cell Biology 2024Quote: Protein concentration was determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific). 15-20 μg of total protein were loaded into 4-15% gradient SDS-PAGE gels (Biorad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were quantified using a Qubit dsDNA HS Assay Kit (Invitrogen cat. no. Q32854) on a Qubit4 Fluorometer and average sizes of each adapter-ligated ...
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bioRxiv - Genetics 2024Quote: ... We quantified the purified samples with the Qubit RNA Broad Range Assay Kit (Invitrogen), then synthesized cDNA using the SuperScript III First-Strand Synthesis System with random hexamers (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... Protein extracts were quantified using a Pierce BCA Protein Assay Kit (Thermo Scientific #23227). A total of 30-50 μg of proteins were separated via SDS-PAGE using Novex™ WedgeWell™ 4-20% ...
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bioRxiv - Developmental Biology 2024Quote: ... Nuclear protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: ... cDNA and library concentrations were analysed using Qubit ds HS Assay Kit (Invitrogen, 2326054) and sample size distribution using Tapestation with high sensitivity D1000 tape (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured via the Pierce BCA Protein Assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions and measured using a spectrophotometer (Tecan ...
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bioRxiv - Biochemistry 2021Quote: Total protein synthesis was quantified with the help of OPP-incorporation assay based on Click-iT™ chemistry using the Click-iT™ Plus OPP-Alexa Fluor™ 488 protein synthesis assay kit (Life Technologies). The assay was carried out in accordance with the manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... Protein content was measured in each well at the end of the assay using the Qubit Protein Assay Kit (Molecular Probes, Eugene, OR, USA). Oxygen consumption rate (OCR ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell proliferation was also assessed by 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay using Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... The amount of BS present in liver extract was normalized to liver protein content as measured by bicinchoninic acid (BCA) assay (Pierce® BCA protein assay kit, Thermo Scientific, Waltham, Massachusetts).
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bioRxiv - Molecular Biology 2024Quote: ... WT human ASNS and two ammonia tunnel variants was determined in a continuous assay format using the EnzChek™ Pyrophosphate Assay Kit (Molecular Probes, Eugene, OR). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... The fibril concentrations reported are in monomer equivalents as determined by a BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific, MA). After incubation ...
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bioRxiv - Cell Biology 2024Quote: ... The total protein concentration of the cleared lysate was then measured by Bradford assay (Pierce™ Bradford Plus Protein Assay Kits, Thermo Fisher Scientific, 23236) at 595 nm using a ClarioStar plate reader ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using the Macherey Nagel Nucleo Spin Tissue DNA kit and quantified using a Qubit fluorometer (Qubit dsDNA BR Assay Kit, Q32853, Invitrogen). Thirty-seven blood samples at T0 (pre-stress ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was then diluted 1:5 in 1x Assay buffer and processed according to the kit instructions (DNA Damage Competitive ELISA Kit, Invitrogen). The initial DNA concentration used in the assay was optimized to ensure the resulting values fall within the optimum range of kit detection limits (between Std3 and Std6) ...
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bioRxiv - Neuroscience 2021Quote: ... and quantity was assessed using Agilent BioAnalyzer RNA 6000 Pico Kit and the Invitrogen Qubit RNA HS Assay kit (Invitrogen), respectively ...
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bioRxiv - Neuroscience 2021Quote: Cell viability was determined using a commercially available dual-wavelength fluorescent kit (LIVE/DEAD Viability/Cytotoxicity Assay Kit; L3224; Invitrogen). Live cells are detected by intracellular esterase activity using the dye calcein-AM (∼495/515 nm ex/em ...
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bioRxiv - Systems Biology 2021Quote: ... DNA was extracted from 1 mL of this outgrown culture using a GeneJet Genomic DNA Purification Kit (ThermoScientific) and the concentration of genomic DNA was quantified using a Qubit dsDNA HS Assay Kit (Invitrogen). A 1 μg aliquot of genomic DNA was suspended in 15 μL of 10 mM Tris buffer ...
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bioRxiv - Microbiology 2024Quote: ... the PCR products were purified using the E-Z 96 Cycle Pure Kit (Omega) and quantified with Qubit dsDNA HS Assay Kits (Invitrogen). The purified PCR products were then pooled equally for subsequent sequencing ...
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bioRxiv - Microbiology 2023Quote: ... after which the entire pool was purified using the Qiagen MinElute purification kit and quantified via Qubit dsDNA HS assay kit (Invitrogen) and sequenced at Novogene on 1 HiSeq PE 150 lane (6 bp ...
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bioRxiv - Microbiology 2023Quote: The protein content of pEVs were quantified using Pierce BCA kit according to the manufacturer’s instructions (BCA Protein Assay Kit, Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: The TaqMan assays were performed using a one-step TaqMan probe kit (TaqManTM RNA-to CTTM 1-Step Kit, Invitrogen). As the TaqMan probe we used 12➔7 Probe FAM–ACCATCAGCCCCCTTTTTATTTCCT-MGBNFQ ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the viability of cells in 3D systems was assessed visually using a live/dead staining kit (LIVE/DEAD Viability/Cytotoxicity Assay Kit, Invitrogen Thermo Fisher Scientific Ref L32250 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was finally developed using the SuperSignal® West Pico Chemiluminescent Substrate Kit (Life Technologies), following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Protein was extracted from tissue membranes using the MEM-PER protein extraction kit (89842, ThermoFisher Scientific). Protein extracts were measured using a DPP4 ELISA kit (DY1180 and DY008 ...
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bioRxiv - Microbiology 2023Quote: ... RNA was transferred to a Nytran Super Charge membrane (Whatman) using the NorthernMaxTM-Gly kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein was examined using Pierce Reversible Protein Stain Kit for PVDF membranes (Thermo Fisher, PI24585) and imaged using Bio-Rad ChemiDoc Imaging System ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were treated with the Pierce™ ECL Western Blotting Substrate kit (Thermo Fisher Scientific, 32106) according to the manufacturer instructions and developed using a Western Blot Fusion FX7 Imager (Vilber).
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bioRxiv - Microbiology 2024Quote: ... and the ΔmelH complemented strain were determined using a BacLight Bacterial Membrane Potential Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... which were subsequently fractionated into cytosolic and membrane fractions using a Subcellular Protein Fractionation Kit (ThermoFisher) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Rheological characterization of the jammed microgel ink and PAM scaffolds was performed using parallel plates geometry (20 mm diameter, 1 mm gap) mounted on strain-controlled rheometers (Thermo Scientific HAAKE MARS 40/60 Rheometer). Jammed microgels were prepared in the same way and molded to an identical geometry to the plates ...
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bioRxiv - Bioengineering 2021Quote: For sender membrane preparation the nitrocellulose membranes (ThermoFisher Scientific, USA, 88018) were cut into squares (8 × 8 mm ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was stained with Novex reversible membrane protein stain (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Dissolved protein was analyzed for protein concentration by micro-scale BCA assay (Micro BCA Protein Assay Kit #23225; Thermo Fisher Scientific Inc., Waltham, MA, USA). Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... Quantitation of protein concentration was estimated by bicinchoninic acid (BCA) assay using the Thermo Scientific™ Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific; 23227).
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bioRxiv - Molecular Biology 2023Quote: ... Bradford assay was performed for determining the protein concentration of each sample using Pierce BCA protein assay kit (Thermo Scientific, Pittsburgh, PA, USA, Ref.23225) and a Tecan Infinite 200 plate reader (Tecan Group Ltd. ...
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bioRxiv - Cancer Biology 2023Quote: ... permeabilized with 1X Proteinase K solution (provided in the Click-It TUNEL Assay kit) followed by TUNEL staining using Click-iT TUNEL Alexa Fluor Imaging Assay for Microscopy (Thermo Fisher Scientific Cat. No. C10245). The OC cells were labeled with Pan-Keratin (1:200 ...