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Citations for Thermo Fisher :
3801 - 3850 of 10000+ citations for Coatomer Subunit Epsilon COPE Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... Cells were further washed and stained with an InlA polyclonal antibody (LSBio, Inc) or an HA monoclonal antibody conjugated with Alexa Fluor 555 (ThermoFisher Scientific) at a 1:100 concentration for 30 min prior to visualization by confocal microscopy ...
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bioRxiv - Plant Biology 2021Quote: ... antibody was used at 1:600 and secondary Alexa Fluor 546 anti-rabbit antibody was used at 1:500 (Molecular Probes).
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bioRxiv - Microbiology 2020Quote: ... Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Microbiology 2021Quote: ... The cells were then incubated for 1 hour with primary antibodies followed by the addition of secondary antibodies conjugated to Alexa Fluor 488 or 594 (Molecular Probes). Nuclei were stained for 10 min at room temperature with Hoechst 33258 at 2 μg/ mL in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were diluted 1:100 and secondary antibodies conjugated to Alexa flour 488-or 568 (Invitrogen, Thermo Fisher Scientific, UK) were diluted 1:500 in 4% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were diluted 1:100 and secondary antibodies conjugated to Alexa flour 488-or 568 (Invitrogen, Thermo Fisher Scientific, UK) were diluted 1:500 in 4% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... the primary antibody was labeled with the Alexa-488 goat anti-mouse IgG secondary antibody (Invitrogen, Life Science, Eugene OR, USA) and fluorescent foci observed and counted with an Olympus BX41 microscope equipped with an UPlanFI 4× objective and a FITC filter ...
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bioRxiv - Microbiology 2021Quote: ... Alexa 488-conjugated goat anti-mouse IgG antibody and Alexa 555-conjugated goat anti-rabbit IgG antibody were purchased from Thermo Fisher Scientific (MA ...
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bioRxiv - Microbiology 2021Quote: ... antibody 1:3000 in 1% PBS/BSA at 4°C followed by secondary antibody conjugated to Alexa Fluor 555 (Thermo Fisher) at 1:500 in PBS/BSA 1% was performed ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... NPC1-eBFP2 was detected using a rabbit anti-BFP antibody followed by a secondary anti-rabbit antibody-Alexa 405 fluorophore conjugate (Thermo Fisher). Cells were examined by immunofluorescence analysis as described under the method section ‘Immunofluorescence microscopy and immunostaining’.
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Cleaved GP was detected with the primary mouse RBS-specific anti-GP antibody MR72 followed by secondary anti-mouse antibody-Alexa 555 fluorophore conjugate (Thermo Scientific). To investigate transport of IgGs bound to VSV particles into NPC1+ endosomal compartments ...
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bioRxiv - Microbiology 2020Quote: ... the bound antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody (1:5,000, Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were blocked using 2.5% normal goat serum for 30 min then incubated in primary antibody for 1hr followed by secondary antibody mouse anti-rabbit Alexa 488 (1:1000, Molecular Probes) for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... GFP antibody and HER2/neu Ab-8 (Clone e2-4001) mouse monoclonal antibody used for IP were obtained from Thermo Fisher. C6orf97 antibody is from GeneTex ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary anti-mouse antibodies labeled either with Alexa Fluor® 488 secondary antibodies (green) or Alexa Fluor® 568 (Red) (Invitrogen) were used at 1:500 dilutions ...
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bioRxiv - Neuroscience 2020Quote: ... with the appropriate secondary antibodies: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Invitrogen, A23766) and Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... with 0.1% Tween-20 in 1X PBS pH 7.4 and then incubated overnight at 4°C with the appropriate secondary antibodies: Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 555 (Invitrogen, A32816) and Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed with PBS and then incubated with secondary antibody Alexa Fluor 488-conjugated goat anti-mouse antibody (Life Technologies) diluted at 1:100 in 5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were then incubated with primary antibodies for 1 hour and then for a further 1 hour with appropriate secondary antibodies conjugated with a fluorophore (Molecular Probes). The antibodies used and respective dilutions are displayed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Conjugated antibodies were then validated signal by IMC in suspension mode (Helios) with antibody capture beads (AbC Total compensation beads, Thermo Fisher). To confirm conjugation did not alter antibody binding efficiency ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody followed by incubation of horseradish peroxidase-linked goat anti-rabbit or anti-mouse secondary antibody (1: 5,000, Thermo Fisher Scientific). Antibody binding was detected using the enhanced chemiluminescence immunoblotting detection system (Perkin Elmer LLC WESTERN LIGHTNING PLUS-ECL ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no. A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Neuroscience 2022Quote: ... Detection of primary antibodies was achieved using secondary antibodies conjugated to Goat anti-mouse Alexa488 (1:400, Thermo Fisher, Cat#A28175), Goat anti-rabbit Alexa488(1:400 ...
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bioRxiv - Genetics 2022Quote: ... antibodies followed by AlexaFluor 488 anti-rabbit and AlexaFluor 568 anti-mouse secondary antibodies (Both at 1:1000; Thermo Fisher Scientific). To visualize the GR8A protein ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS with 0.05% Tween-20 (PBST) and incubated in primary antibody and respective secondary antibody (Alexa Fluor; Thermo Fisher Scientific) in blocking buffer diluted 1:5 with PBST ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of Kir2.1EGFP was assessed with anti-GFP antibody (rat monoclonal Ab from Nacalai, Japan, GF090R; the secondary antibody, 488-anti Rat IgG from Invitrogen, A11006) (Mizuno et al. ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with anti-Sgs1 antibody (dilution 1:500) and anti-V5 antibody (dilution 1:1000, Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were washed three times in PBST and incubated for one hour at room temperature with the fluorescent secondary antibody (Goat anti-mouse or Goat anti-rabbit antibodies conjugated with either Alexa488 or Alexa555, 1:400 dilution, Life technologies) in the same blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... The lysates were then resolved by SDS-PAGE and immunoblotted with indicated antibodies (Wapl and V5-tag antibodies were purchased from Thermo Fisher Scientific #PA5-38024 and #R960-25 ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-PrimPol antibody (Table 1) was coupled to magnetic M-270 Epoxy Dynabeads® (7.5 μg antibody/ mg beads; Life Technologies), and IPs were performed following manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Western blots were carried out using iBind with 1 ug/ml primary antibodies and 1 ug/ml secondary antibodies following iBind protocol (Invitrogen/ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were visualized using donkey anti-rabbit antibody conjugated with Alexa Fluor Plus 555 (1:1000, #A32794, Thermo Fisher Scientific) and donkey anti-goat antibody conjugated with Alexa Fluor Plus 488 (1:1000 ...
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bioRxiv - Systems Biology 2020Quote: ... Visualization of antibody signals was achieved using HRP-conjugated secondary antibodies and the chemiluminescence substrate SuperSignal West Pico (Thermo Fisher Scientific). Blots were imaged and quantitatively analyzed using a Chemidoc MP imager (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: The primary antibodies were as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183); rabbit anti-HA tag (C29F4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 2 washes to remove any excess antibody cells were stained with anti-Rabbit Alexa-546 antibody (Life technologies, Cat#A11035). After 3 washes to remove any non-specific antibody nuclei were stained using Hoechst at 1:50,000 dilution and imaged on a Nikon Ti Eclipse automated microscope ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with PBS and then incubated with secondary antibody (1:1000 goat anti-rabbit antibody or goat anti-mouse antibody conjugated to Texas Red and 1:1000 goat anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies) and 1:1000 donkey anti-goat antibody conjugate to Texas Red (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine ...
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bioRxiv - Developmental Biology 2020Quote: ... primary antibodies were detected using appropriate secondary antibodies coupled to Alexa-Fluor 488 and Alexa Fluor 594 (1:100; Molecular Probes) and diluted in PBTr for 2 days at 4°C.
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bioRxiv - Pathology 2020Quote: ... The binding sites of the primary antibodies were revealed with Alexa Fluor-594 goat anti-rabbit and Alexa Fluor-488 goat anti-mouse secondary antibodies (Invitrogen, USA). Nuclei were stained with 4’ ...
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Pathology 2020Quote: ... The secondary antibodies used to reveal LC3 and LAMP2 were Alexa 594-labeled anti-rabbit secondary antibody and Alexa 546-labeled anti-mouse secondary antibody (Invitrogen, USA), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed thrice in PBS containing 0.05% Tween 20 and incubated with secondary antibody (anti-mouse antibody conjugated to Alexa 647 or Alexa 546, Thermo Fisher Scientific) at 1:4000 dilution and GFP nano-booster (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... or rabbit TgALD1 antibody (1:200, kind gift from Dr. Kentaro Kato) and anti-rabbit HRP antibodies (Thermo Fisher, 1:10000) followed by development of signal with West Pico Plus Chemiluminescent Substrate (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2021Quote: ... as the primary antibody at a 1:100 dilution and anti-mouse IgG Alexa Fluor 488 as the secondary antibody (A11029, Life Technologies) at a 1:100 dilution ...
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bioRxiv - Microbiology 2020Quote: ... and rabbit anti-SERA5 (1:500) (54) primary antibodies in combination with appropriate fluorophore-coupled secondary antibodies (1:1,000; Thermo Fisher Scientific). Stainings with Lysosensor Blue DND-167 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: The rabbit α-NOCT polyclonal antibody was produced in rabbits inoculated with MBP-NOCT (1-431) (Thermo Fisher custom antibody services). This MBP-NOCT antigen (1-431 ...
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bioRxiv - Neuroscience 2020Quote: ... to magnetic beads at a final concentration of 10 mg of antibody / mL of beads was done following the Magnetic Dynabeads Antibody Coupling Kit from Invitrogen (14311D). Human tissue lysates were also prepared using the “Sequential Extraction of Proteins from Tissue” protocol as described above with addition of 10 mM iodoacetamide prior to homogenization ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated in secondary antibodies (Alexa Fluor 488-, 555-, 594-, 647-conjugated secondary antibodies, or anti-mouse IgG isotype-specific secondary antibodies; 1:500; Life Technologies, Carlsbad ...
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bioRxiv - Immunology 2021Quote: ... and incubated with the secondary antibody (Alexa-Fluor 647- or Alexa-Fluor 405-conjugated goat anti-mouse Ig antibodies; Invitrogen, France) for 45 min at room temperature ...