Labshake search
Citations for Thermo Fisher :
3751 - 3800 of 6996 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Total RNA was isolated from human desmoid tumor cell lines using Trizol reagent (Invitrogen) according to the manufacturer instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primed human ESC and iPSC cells were harvested with 0.25% trypsin-EDTA solution (Invitrogen), and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System ...
-
bioRxiv - Microbiology 2022Quote: ... human foreskin fibroblast (HFF) monolayers were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 25 mM glucose (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
-
bioRxiv - Molecular Biology 2021Quote: ... slides were incubated with Alexa Fluor 488 goat anti-human IgG(H+L) (Invitrogen) at 1:500 (in 3% BSA/PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... except for human XBP1S that was assessed using SYBR green master mix (Thermofisher Scientific) using primers in Supplementary Table 3.
-
bioRxiv - Molecular Biology 2021Quote: ... Stable integration of CLIP-UPF1SL into human Flp-InTM T-RExTM-293 cells (Invitrogen) and subsequent maintenance of this stable line was previously described (Kishor et al ...
-
bioRxiv - Genomics 2020Quote: The THP1 (human acute monocytic leukemia derived; ATCC number TIB-202 purchased from Thermofisher) cell line[18] was regularly maintained in RPMI 1640 medium containing GlutaMAX™ ...
-
bioRxiv - Genomics 2020Quote: ... Samples passing quality control were analyzed using the human Clariom D Assay (ThermoFisher Scientific) and analyzed using Transcriptome Analysis Console (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2019Quote: ... and stained with Alexa 647-conjugated anti-human IgG (H+L) antibody (Thermo Fisher) for 1h at 4°C ...
-
bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA ...
-
bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
-
bioRxiv - Biophysics 2019Quote: Homo sapiens SUR1 and human Kir6.2 were subcloned into pcDNA4/TO (Invitrogen; Carlsbad, CA) for expression in HEK-293T cells ...
-
bioRxiv - Genomics 2019Quote: ... dataset (which used an Affymetrix platform, [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array); the default importer in BRB-ArrayTools was used to collate the data from .cel files ...
-
bioRxiv - Immunology 2019Quote: ... anti-human CD3 conjugated to FITC (clone OKT3, cat. no. 11-0037-41, Invitrogen), anti-human CD8 conjugated to Pacific Blue (clone 3B5 ...
-
bioRxiv - Immunology 2019Quote: ... Cortactin expression was assessed using rabbit anti-human cortactin antibody (PA5-27134; Life Technologies) stained in conjugation with goat anti-rabbit Cy3 (Life Technologies) ...
-
bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
-
bioRxiv - Immunology 2019Quote: ... and the ELISPOT plate was incubated with biotinylated goat anti-human IgG Fc (Invitrogen), followed by HRP-conjugated avidin (Vector Laboratories ...
-
bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
-
bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
-
bioRxiv - Genomics 2019Quote: ... We used the Genome-Wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA, USA) for genotyping single nucleotide polymorphisms (SNPs) ...
-
bioRxiv - Immunology 2021Quote: ... coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher, #H17000) in 0.1 M carbonate buffer ...
-
bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific) and cultured in DMEM medium (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2021Quote: ... followed by the secondary goat anti-human HRP-conjugated antibody (1:4000, A18805, Invitrogen). Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
-
bioRxiv - Biochemistry 2020Quote: Human GCDH cDNA (clone ID 3142978) in pOTB7 vector was obtained from Thermo Fisher Scientific Open Biosystems repository (now part of Horizon Discovery) ...
-
bioRxiv - Biochemistry 2020Quote: ... 7.5 μg/mL human IgG and 50 μg/mL BSA-biotin (Thermo Fisher Scientific) were also printed to serve as a printing control and “intra-well signal normalizer” ...
-
bioRxiv - Biochemistry 2020Quote: ... and negative selection using the Dynabeads Untouched Human CD4 T Cells kit (Invitrogen 11346D) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: Human embryonic kidney (HEK)293T cells (ATCC, CRL-11268) were grown in DMEM (Gibco) plus 10% heat-inactivated fetal calf serum (FCS ...
-
bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
-
bioRxiv - Biophysics 2021Quote: ... CD4+ T cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) and cultured in AIM V Medium (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Biophysics 2021Quote: ... The human Gβ1 and Gγ2 subunits were integrated into the pFastBac Dual vector (Invitrogen) with an N-terminal 6 × His-tag at the N terminus of Gβ1 ...
-
bioRxiv - Cancer Biology 2020Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher, Waltham, MA) at a 2:1 bead:cell ratio for 48 h prior to electroporation ...
-
bioRxiv - Cancer Biology 2019Quote: ... and two independent human GRPR siRNAs (Santa Cruz, # sc-106924 and Life Technologies, #145216), human GRPR shRNA in Lentiviral Vector (Genomics Online ...
-
bioRxiv - Cell Biology 2021Quote: Undifferentiated human neuroblastoma SH-SY5Y cells were cultured in DMEM/F12 medium (Gibco, 11320033) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Rb polyclonal antibody anti-GMAP-210 (proteintech) or Ms monoclonal anti-Trip230 human (Invitrogen). Images were acquired on a Leica confocal TCS SP8 STED microscope ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1ẞ high sensitivity kit sold by Invitrogen. The sample for our ELISA consisted of cell culture media (referred to as sample hereafter ...
-
bioRxiv - Bioengineering 2021Quote: ... 100 μL of 5 μg/mL fibrinogen from human plasma conjugated to AF488 (Invitrogen) was added to each well ...
-
bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2020Quote: ... anti-human CD20 mouse IgG2 monoclonal antibody clone 2H7 (Invitrogen, cat: 14-0209-82), anti-human CD38 chimeric monoclonal antibody kindly provided by Centrose LLC ...
-
bioRxiv - Microbiology 2021Quote: ... Goat anti-human and anti-mouse antibodies pre-coupled to Alexa Fluor 647 (Invitrogen) were used as secondary antibodies in flow cytometry experiments ...
-
bioRxiv - Microbiology 2021Quote: ... A mating strategy was used to screen a commercial human spleen cDNA library (Invitrogen) established in the pPC86 vector to express cellular proteins in fusion downstream of the GAL4 transactivation domain (GAL4-AD) ...
-
bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
-
bioRxiv - Microbiology 2021Quote: ... Human DDX42 cDNA was amplified by RT-PCR using the SuperScript III™ (Invitrogen) from mRNAs of MDMs using primers DDX42-forward 5’-AATTAATTTAGGATCCATGAACTGGAATAAAGGTGGTCCTG and DDX42-reverse 5’-AATTAATTTACTCGAGCTAACTGTCCCATCGACTTTTCTTGCG ...
-
bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNA from mouse and human atrial tissue was extracted using Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... The Taqman probes used for human and mouse genes were purchased from Applied Biosystems and listed in Table S5.
-
bioRxiv - Microbiology 2019Quote: ... Human lung epithelial A549 cells were maintained in Gibco’s F-12 medium (Life Technologies). MDCK and A549 cells were obtained from Dr ...
-
bioRxiv - Cancer Biology 2020Quote: RNA was extracted from human breast tissue after homogenization into TRIzol (Ambion, Life Technologies) using Qiagen Tissue Lyser II with metal beads ...
-
bioRxiv - Cancer Biology 2020Quote: RNA was extracted from human breast tissue after homogenization into TRIzol (Ambion, Life Technologies) using Qiagen Tissue Lyser II with metal beads ...