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Citations for Thermo Fisher :
3751 - 3800 of 5181 citations for S100A12 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: The TR146 human buccal epithelial squamous cell carcinoma cells (MilliporeSigma) 50 were incubated in DMEM/F12 medium (Gibco, #11320033) with 10% fetal bovine serum and 1x penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: T24 human epithelial bladder cells (ATCC HTB-4) were grown in McCoy’s 5A modified medium (Gibco 16-600-082) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... activated with anti-CD3/anti-CD28 activation beads per manufacturer’s instructions (Gibco Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation, Thermofisher), stimulated with IL-2 (R&D) ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated from human samples using Ficoll-Paque density centrifugation (Corning, Fisher Scientific, CA). PBMC were cultured in RPMI 1640 medium (Corning ...
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bioRxiv - Immunology 2024Quote: ... and Spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT were co-transfected into human embryonic kidney (HEK)293 T cells (ATCC CRL_3216) with lipofectamine 2000 (ThermoFisher Scientific). Pseudo-typed viruses of SARS-CoV-2 variants were generated using the Spike protein from WA1/2020 (Wuhan/WIV04/2019 ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a secondary Alexa Fluor 647 conjugated goat anti-human IgG (H+L) antibody (Invitrogen #A-21445; 10 μg/mL) was used.
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bioRxiv - Molecular Biology 2024Quote: ... All human L1 plasmids contain the indicated fragments of L1.3 DNA (a retrotransposition-competent human L1, GenBank accession no. L19088 (32)) cloned into pCEP4 (Invitrogen) unless indicated otherwise ...
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma HCT-8 cells (ATCC) and THP-1 cells (ATCC) were maintained in Advanced RPMI 1640 (ThermoFisher Scientific) supplemented with 10% fetal bovine serum and 1X penicillin-streptomycin (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...
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bioRxiv - Biophysics 2021Quote: A stable β2AR-overexpressing cell line was produced by transfecting CHO-K1 cells (ECACC) with a plasmid coding for the human β2AR with C-terminal GFP using lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The concentrations of IgG in cell lysates and supernatants after recycling were measured with an IgG Human ELISA Kit (88-50550, Invitrogen), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... the culture medium was switched to standard human embryonic stem cell (hESC) medium containing DMEM/F12 (Thermo Fisher Scientific, 11320033), KnockOut Serum Replacement (20% ...
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bioRxiv - Cell Biology 2020Quote: ... Each labeled sample was assayed with the Affymetrix PrimeView human gene expression array read with an Affymetrix GeneChip Scanner 3000 (Affymetrix/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... cells were subsequently pulsed at 370C for different time points with 25µg/ml Alexa flour-488 or- 568-conjugated human transferrin (Invitrogen, ThermoFisher Scientific) reconstituted in starvation medium and clarified by centrifugation at 15,000 × gmax at 40C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Bovine or human TLR2 sequences of interest were cloned into pcDNA3.3 TOPO TA mammalian expression vector (Thermo Fisher Scientific, UK), and transfected into SEAP HEK cells using TurboFect transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... The extraction and purification of human genomic DNA were performed using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific) and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Immunology 2020Quote: Frozen human peripheral blood mononuclear cells (PBMCs) from blood donors were thawed and diluted into cold RPMI 1640 medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2020Quote: ... and the secondary antibodies including horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (#31439) and HRP-conjugated goat anti-human IgG (#31410) were purchased from Invitrogen. The secondary antibody for HRP-conjugated goat Anti-rabbit IgG (#12-348 ...
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bioRxiv - Genomics 2020Quote: Primary human keratinocytes were isolated from fresh surgically discarded neonatal foreskin and cultured in Keratinocyte-SFM (Life Technologies 17005-142) and Medium 154 (Life Technologies M-154-500) ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Physiology 2022Quote: ... human TACAN or their mutants were synthesized by in vitro transcription using the T7 mMESSAGE mMACHINE kit (Invitrogen, Waltham, MA) and injected into oocytes (25 ng each) ...
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bioRxiv - Systems Biology 2022Quote: ... RNA from human skin and wounds was reverse transcribed using TaqMan® Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific). Individual miRNA expression was then quantified using TaqMan® Advanced miRNA Assays (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Ambion™ Silencer™ Pre-Designed siRNA specific of human GLUT3 (SLC2A3) was purchased from Fisher Scientific (#10446914, Illkirch, France)
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bioRxiv - Neuroscience 2021Quote: DIV80 human iPSC-derived astrocytes were incubated with the calcium indicator Fluo-4 AM (1μM; ThermoFisher Scientific; Cat. No. F14201) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with the following primary antibodies: rabbit anti-Profilin 1 (Novus Biologicals, NB200-262, 1:10’000 for human lysates; Invitrogen, PA5- 17444 ...
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bioRxiv - Immunology 2022Quote: Total or individual CD8+ or CD4+ T cells were purified from PBMC suspensions from our PLWH and HIV-1 negative control study cohorts by negative immunomagnetic selection (purity >90%) using the Untouched Human T cell and CD4 T cell Dynabeads Kits (Invitrogen) and the MojoSort Human CD8 T cell (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2019Quote: ... and MΦs were differentiated by 12-days adherence culture in RPMI 1640 media containing 10% human serum and 1x cell culture pen/strep antibiotic mix (Gibco). All cells were deidentified before receipt ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Neuroscience 2020Quote: ... The next day when cells were at 60–70% confluency and were transduced with 10 µl of lysate normalized to 3ng/µl per well of tau from rTg4510 mice or 5ng/µl per well of tau from human brains plus 1% Lipofectamine 2000 (Invitrogen) diluted in OptiMEM (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA synthesis was performed with Superscript III and used for qPCR according to manufacturer’s protocol with TaqMan human iPS cell Scorecard assay (Life Technologies). Data analysis was performed with Scorecard software (online tool Life technologies) ...
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bioRxiv - Developmental Biology 2019Quote: Human genital ridges were dissected in PBS and separated from the mesonephros followed by dissociation with TrypLE Express (Life Technologies) at 37°C for 20-40 minutes (depending on the tissue size ...
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bioRxiv - Genomics 2020Quote: ... Both cell lines were maintained in culture vessels coated with a matrix of Vitronectin human recombinant protein (ThermoFisher Scientific, A14700) and batch fed daily with mTeSR (STEMCELL Technologies ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was transfected into human osteosarcoma U-2 (U2OS) cells by electroporation using the Neon Transfection System (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... were used to retro-transcribe 1 μg of total RNA from Human Liver Total RNA (ThermoFisher Scientific, Waltham, MA, USA). The reverse transcriptase reaction for the viral pellet was carried out with random primers using a commercial kit (EasyScript cDNA Synthesis kit ...
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bioRxiv - Neuroscience 2019Quote: ... or 1 µg of pCMV-SPORT6.FBXL3 and 40 nM ON-TARGETplus human CUL1 (8454) siRNA SMARTpool (Dharmacon, L-004086) using the Neon transfection system (Invitrogen) following the manufacturer instructions and 1400 V ...
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bioRxiv - Biophysics 2019Quote: Jurkat T cells (Human T cell leukemia cell line– ATCC TIB-152) were grown in RPMI-1640 with Glutamax (GIBCO), supplemented with 10 % foetal calf serum and ...
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bioRxiv - Biochemistry 2020Quote: The heavy and light chain sequences of mAb45D825 were separately cloned into pcDNA3.4 and the resulting vectors were transfected into Expi293F Human Embryonic Kidney cells (Life Technologies) using a 1:2 mass ratio of light and heavy chain DNA with the Expifectamine transfection kit (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... falciparum cell lines were cultured in 5% human O+ red blood cells (Australian Red Cross blood service) in RPMI-GlutaMAX-HEPES (Invitrogen) supplemented with 5% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... an in-house generated model of human hepatic Huh-7 cells stably overexpressing mRFP-Rab5 were cultured in DMEM/GlutaMax/High glucose (Gibco) supplemented with 10 % FBS ...
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bioRxiv - Bioengineering 2019Quote: ... envelope and one of these transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s lentiviral production protocol (Thermo Fisher Scientific Inc. ...
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bioRxiv - Developmental Biology 2019Quote: Human astrocytoma-derived 1718 cells [CCF-STTG1 (ATCC® CRL-1718TM)] were cultured in RPMI 1640 medium (ATCC modification, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2019Quote: U-2 OS cells (human bone osteosarcoma epithelial cells, ATCC Number: HTB-96 [17]) were grown in D-MEM Glutamax (Gibco) supplemented with final concentrations of 10% fetal calf serum (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: ... Primary neonatal Human Umbilical Vein Endothelial Cells (HUVEC) were sourced from three suppliers to provide genetic diversity (Lonza, Gibco, PromoCell). HUVECs were expanded in endothelial cell growth medium (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... and CD19+ B-cells were isolated by negative selection using the Dynabeads® Untouched™ Human B Cells kit (Invitrogen). Mantle cell lines (Z-138 ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2019Quote: ... Immortalized human umbilical vein endothelial cells (iHUVEC, gift from Dr. W. Muller, Northwestern University [NU]) were cultured in human endothelial serum-free medium (Gibco) supplemented with 10% FBS ...