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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... followed by three washes with 1% FCS/PBS and secondary staining using Alexa-546-anti-rabbit (Invitrogen, A11035), Alexa-488-anti-mouse (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), Epcam-BV421 (clone G8.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... were grown in DMEM (Dulbecco’s Modified Eagle’s Medium, Hyclone) supplemented with 10% (v/v) FCS (Fetal Calf Serum, Gibco), 3.7 g/l sodium bicarbonate ...
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bioRxiv - Cancer Biology 2023Quote: Viably frozen samples obtained from patients diagnosed with AML or normal CD34+ hematopoietic progenitor cells were thawed in 37°C water bath as fast as possible and placed immediately in 20% FCS Iscove’s Modified Dulbecco’s Medium (Gibco IMDM ...
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bioRxiv - Cancer Biology 2023Quote: ... suspended in 20μl sterile complete media (MEM-Alpha (Biological Industries)) supplemented with 10% Fetal Calf Serum (FCS) (Gibco) and 1% L-Glutamine/Penicillin/Streptomycin (Biological Industries) ...
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bioRxiv - Immunology 2024Quote: ... and absorbance was measured at 450 nm and 570 nm using the Multiskan FC Microplate Photometer (Thermo Scientific). To correct for optical imperfections ...
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bioRxiv - Molecular Biology 2024Quote: In order to block non-specific Fc-mediated interaction,1 μg of normal Rabbit IgG (ThermoFisher Cat: 31235) is added to the cell sample and incubated for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... and the absorbance at 750 nm was measured using a plate reader (Multiskan FC, ThermoFisher, Waltham, MA, USA). Total polyphenols content was expressed as gallic acid equivalent per gram of watermelon flesh sample.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAX [Gibco] supplemented with 5% fetal bovine calf serum (FCS; [Gibco]) and 50 μg/mL gentamicin [Gibco] ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- D-glucose (2-NBDG) (Invitrogen N13195) was used as a probe for monitoring glucose uptake ...
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bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: The human anti-DNP and anti-WTA GlcNAc-β-4497 monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (21) ...
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bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
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bioRxiv - Bioengineering 2020Quote: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041).
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bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: Silencer Select siRNA negative control (4390844) and those targeting GFAT1 (human, s5708; mouse, s66610) or GFAT2 (human, s19305) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... Other monoclonal human anti-DNP and anti-WTA IgG of the four IgG subclasses were produced recombinantly in human Expi293F cells (Life Technologies) as described before 51 with minor changes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell binding experiment was done with the human T lymphocyte cell Jurkat and anti-human IgG antibody-Ax488 (ThermoFisher Scientific). Ax488 signal changes upon binding were detected using a flow cytometer (BD Accuri C6 ...
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bioRxiv - Immunology 2023Quote: ... Human serum used for all conditions was from the same lot of pooled human AB serum (Fisher Scientific, Cat#: BP2525-100). For heat inactivation ...
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bioRxiv - Cell Biology 2024Quote: HepG2 (Human hepatoma cell line, ATCC, Manassas, Virginia, United States of America) and HEK293A (Human embryonic kidney cell line, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... non-metabolizable glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Thermo Fisher Scientific, cat.no. 11569116). Samples were prepared as described in the section ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were detected by Coomassie Blue staining or Western blotting using anti-His6-tag antibodies (Thermo Fisher Scientific MA1-21315).
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-V5-tag to amplify V5-tagged CFP (Thermo Fisher, R96025, 1:650), rat anti-mCherry (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... kcnk5b cDNA was isolated by RT-PCR from regenerating adult fin cDNA library and cloned into MCS region of pcDNA6-myc-6xHIS-tag plasmid (Invitrogen) or pBluescript harboring the hsp70 zebrafish promoter and GFP coding sequence surrounded by 2 miniTol2 sites ...
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bioRxiv - Immunology 2021Quote: ... His tag) (BLPSN-0986P, BETA Life Sciences, Fairfield, NJ, USA) was immobilized on a 96-well plate (442404, MAXISORP F96 NUNC Immuno-plate ...
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bioRxiv - Plant Biology 2022Quote: ... AtSUMO1 was cloned into destination vector pK7WGR2 (N-terminal mRFP tag, (Karimi et al., 2002)) by LR reactions using Gateway technology (Invitrogen). Vector specific primer p35s-F used in plasmid construction are listed in Table S2.
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bioRxiv - Microbiology 2022Quote: ... The two pAcgp67-RBD (residues 333–530) plasmid with a C-terminal 8×His tag were transfected into Sf9 cells using Cellfectin II Reagent (Invitrogen) to produce the recombinant baculoviruses ...
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bioRxiv - Neuroscience 2021Quote: ... of mouse Lsm10 and Lsm11 were amplified from a cDNA library generated from NIH3T3 fibroblasts and cloned into pcDNA3 as fusions with a FLAG tag sequence at the amino terminus (Invitrogen). The resulting FLAG-tagged ORFs were then cloned into pRRL.PGK.IRES.Neo and pRRL.PGK.IRES.Hygro lentiviral vectors ...
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: Samples were prepared at the EMBL-Heidelberg Proteomics Core Facility with the SP3 protocol71 and trypsin digested peptides analyzed by liquid chromatography followed by tandem mass tag (TMT)-labeled mass spectrometry using TMT10plex isobaric label reagent set (ThermoFisher). Raw data were mapped and quantified using MaxQuant (v1.6.3.4 ...
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bioRxiv - Biochemistry 2019Quote: ... pET151/D-TOPO plasmids with the sequences of interest inserted under control of Lac operon and T7 viral promoter with N-terminal hexa-histidine tag and Ampicillin selection marker was obtained from Invitrogen. Sequences were optimized for expression in E ...
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bioRxiv - Biophysics 2020Quote: A full-length cDNA encoding the methyltransferase SUV39H1 was amplified by PCR and fused with an N-terminal His6-MBP tag in pET-Duet1 (Invitrogen).
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bioRxiv - Biochemistry 2019Quote: ... the polynucleotides encoding GtACR1 mutants were fused in frame with a C-terminal eight-His tag and subcloned into the pPIC9K vector (Invitrogen) between EcoRI and NotI sites ...
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bioRxiv - Genomics 2019Quote: ... cDNA was fragmented and labeled with a 3’ biotin tag before quantifying gene expression using Affymetrix custom tiling arrays (A-AFFY-116 - Affymetrix Custom Array - S ...
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bioRxiv - Cell Biology 2019Quote: ... The ESCO2 cDNA was cloned into a pcDNA5/FRT-based Flag-tag vector and co-transfected along with a plasmid expressing the FLP recombinase (pOG44, Invitrogen) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... The peptide samples derived from individual patients were then individually labelled using the TMT10plex mass tag kit (Thermo scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Isobaric labeling of the samples was performed using 10-plex tandem mass tag (TMT) reagents (Thermo Fisher Scientific, Rockford, IL). All individual and pooled samples were labeled according to the TMT 10-plex reagent kit instructions ...
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bioRxiv - Microbiology 2021Quote: ... with a N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The recombinant baculoviruses were generated according to the manufacture’s instruction to infect Hi5 cells at a density of 2×106 cells/ml ...
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bioRxiv - Biophysics 2021Quote: The bacteriophage T7 gp15 gene was inserted into the pRSET-B vector (ampr and 6x-His tag) from Invitrogen (USA), between the XhoI and EcoRI restriction sites ...
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bioRxiv - Biophysics 2022Quote: ... and CpE with C-terminal decahistidine tags preceded by thrombin cleavage sites (claudin-4-His10, cCpE-His10, and CpE-His10) were cloned into pFastBac1 (ThermoFisher) and expressed in Tn5 (Trichoplusia ni ...
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bioRxiv - Microbiology 2020Quote: ... vatpas_enh3 and caudal tailed with T7 promoter (TAA TAC GAC TCA CTA TAG GGA GA) were used to synthesize dsRNAs using MEGAscript T7 High Yield Transcription kit (Invitrogen). Four to six-day-old females received a total 69 nl dsRNAs (4 μg/μl ...