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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: We homogenized nasal turbinates and lung tissue samples in 1:20 (w/v) of tissue protein extraction reagent (Thermo Fisher, # 78510), then centrifuged them to pellet tissue debris ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT and 200 mM NaCl containing 2.5 μL of 1/250 diluted SYPRO orange (Applied Biosystems Protein Thermal Shift, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Immunology 2020Quote: ... The solution was allowed to mix at room temperature for 1 h and then added to recently washed and dried magnetic protein G Dynabeads (Thermo Fisher). The mixture was incubated for 1.5 h and resin was separated from the supernatant magnetically ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Immunology 2021Quote: ... yoelii AMA-1 or MSP-119 proteins in sodium bicarbonate buffer were used to coat high binding Immunlon HBX plates (Thermo Scientific) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... soluble form of HO2 spanning residues 1-248 (the core of the protein) was expressed from the pET28a vector in BL21(DE) cells (Life Technologies) and purified by Ni-NTA– agarose affinity chromatography (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... Denatured tri-S protein (50 µg total) was separated by SDS-PAGE with a NuPAGE® 4-12% Bis-Tris Gel (1-well, Invitrogen), electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Immunology 2022Quote: ... Sections were then incubated with cocktails of primary antibodies: rabbit anti-SARS-CoV-2 Spike Protein (1:100, Invitrogen, #MA5-36087) + rat anti-ERα H222 (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... bound proteins were denatured by addition of 1 bed volume of 2x LDS buffer supplemented with 1x sample reducing agent (Life Technologies) and incubated at 70°C for 5 minutes.
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bioRxiv - Biochemistry 2019Quote: ... The proteins were solubilized (pipet mixing) with an appropriate amount of 1 % mass fraction sodium deoxycholate (SDC; Thermo Scientific, lot # SJ2450944) in 50 mmol/L ammonium bicarbonate resulting in a 10 µg/µL solution ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were solubilized (pipet mixing) with an appropriate amount of 1 % mass fraction sodium deoxycholate (SDC; Thermo Scientific, lot # SJ2450944) in 50 mmol/L ammonium bicarbonate resulting in a 10 µg/µL solution ...
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bioRxiv - Cell Biology 2019Quote: ... De novo synthesis of Sgo1 protein was quenched by the addition of 2% glucose and 1 mg/mL cycloheximide (Acros Organics).
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bioRxiv - Immunology 2019Quote: ... negative murine myeloma cells at ratio 5:1 by slowly adding poly ethylene glycol (PEG) to the cells pellet and Protein Free Hybridoma Media (PFHM) (Gibco, 12040077) supplemented with 20% Heat inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... spleen cells were stimulated for 4 h with plate bound anti-NK1.1 in the presence of 1 × protein transport inhibitor cocktail (PTIC) containing Brefeldin A/Monensin (eBioscience, Thermo Fisher Scientific) and anti-CD107a (eBio1D4B ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A magnetic beads were pre-blocked for 1 hour at RT with 70 μg Yeast t-RNA (Thermo Fisher Scientific). Sequencing libraries were prepared using the NEBNext DNA ultra kit (E7370 ...
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bioRxiv - Molecular Biology 2020Quote: ... and working solutions of lysates at concentrations of 15-30 µg total proteins per 30 µl were prepared with 1× lithium dodecyl sulfate (LDS) sample buffer with reducing reagent (NuPAGE, ThermoFisher Scientific) followed by heating at 70 °C for 20 min on a Thermomixer (Eppendorf) ...
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bioRxiv - Plant Biology 2020Quote: ... Protein was detected using chemiluminescence with 1:3000 Anti-Guinea pig secondary HRP-coupled antibody (Thermo Fisher Sci Cat No. A18769). Membrane was stripped and tubulin was detected as a loading control by incubation with 1:25,000 Mouse monoclonal anti-TUB DM1A (Abcam) ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin with antibody were rotated overnight at 4 °C before 1:40 protein A/G agarose beads (Thermo Fisher Scientific, #20423) were added and incubated for another 6 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... the samples were boiled at 95°C for 5 minutes before loading 1 % on a 10 % Tris-Glycine SDS-PAGE gel with 10 µL PageRuler Plus Prestained Protein Ladder (Thermo Scientific). After approximately 1 ½ hours electrophoresis proteins were immobilized on an Immobilon-P Transfer Membrane (EMD Milipore ...
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bioRxiv - Genetics 2021Quote: ... We used approximately 16 ug of nuclear protein extract from Capan-1 cells purified using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) per binding reaction.
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bioRxiv - Evolutionary Biology 2021Quote: ... surface proteins were labeled with biotin by adding 1 mg/ml membrane impermeant EZ-LinkTM Sulfo-NHS-SS-Biotin (Thermo Fisher) in 137 mM NaCl and 15 mM sodium borate ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein expression was induced by the addition of 50 μM Isopropyl β-D-1-thiogalactopyranoside (Fisher Scientific, Fair Lawn, NJ USA) to bacterial cell cultures for 2.5 hours with shaking at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed and collected by scraping and subjected to lysis in 1X PBS + 1% SDS as described in a previously reported protocol.17 Protein concentrations were determined using BCA assay (Thermo Scientific).
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bioRxiv - Biochemistry 2022Quote: Purified protein at a concentration of 1 mg/ml was chemically biotinylated using EZ link NHS-PEG4-Biotin (Thermo Scientific, A39259) at a 10-30 times molar excess ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with cold 1×PBS and the chromatin-bound proteins were extracted using the subcellular fractionation kit (Thermo Fisher). Protein concentrations were measured by the BCA assay kit (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... syp-mCh and SSF-tagged opioid receptors using the lipofectamine method were incubated for 15 minutes with Alexa-647 conjugated anti-FLAG antibody (1/1000, M1 antibody from Sigma, Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher) before neurons were washed 3 times with HBS and mounted on the microscope in HBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions containing YejA protein were collected and concentrated to 10 mg·mL-1 using a 50 kDa MW cut-off concentrator (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: BHK-21 cells were transfected with 1 μg of pCAGGS plasmid encoding the NL09 wild-type or escape mutant HA proteins using Lipofectamine (Invitrogen, USA) and Plus reagent (Invitrogen ...
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bioRxiv - Physiology 2024Quote: Titin isoforms were separated on a 1% w/v agarose gel and stained with SYPRO Ruby protein gel stain (Invitrogen, ThermoFisher Scientific) as previously described.30 All samples were measured in triplicate and the average of triplicate measurements per sample was shown.
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bioRxiv - Cell Biology 2024Quote: ... Expression of NS4B-APEX protein was induced by incubating the cells with 1 ng/ml of doxycycline (Invitrogen, Thermo Fisher Scientific) for 16 hours.
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bioRxiv - Cell Biology 2024Quote: ... Expression of NS4B-APEX protein was induced by incubating the cells with 1 ng/ml of doxycycline (Invitrogen, Thermo Fisher Scientific) for 16 hours.
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bioRxiv - Bioengineering 2024Quote: ... Membranes were washed three times with 1× PBST and proteins were detected by chemiluminescence according to manufacturer’s protocol (#RPN2232, Thermo Fisher Scientific). The following antibodies were used ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed twice with distilled water and incubated with a 1:3,000 dilution of protein A-HRP (Thermo Fisher Scientific). After 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Total biotinylated proteins in samples were detected with Alexa Fluor™ 790 Streptavidin Conjugate (1:20 000, S11378, Thermo Fisher Scientific). Band densities were quantified using Image Studio quantification software (LI-COR Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... Bicinchoninic acid (BCA) assay kit for protein quantification and MitoProbe JC-1 Assay kit for flow cytometry were procured from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The immunoprecipitated proteins were run on 4-12 % SDS-PAGE gel by 1 cm from the well and stained with SimplyBlue (Thermo Fisher) for in-gel digestion ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 1 h at 4°C with Pierce™ Protein G magnetic beads (Invitrogen) previously attached to V5 tag antibodies (R960-25) ...
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bioRxiv - Microbiology 2023Quote: ... followed by HRP-labeled anti-mouse IgG (1:50000 dilution) and quantified with Pierce™ BCA Protein Assay Kit (Life Technologies). GST-PfPP1c and PfPP1c were produced as previously described (Hollin et al. ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Cell Biology 2023Quote: ... biopsies were co-cultured with 3T3-J2 fibroblasts and Rho-associated protein kinase inhibitor (Y-27632) in epithelial cell expansion medium consisting of a 3:1 ratio DMEM:F12 (Gibco, UK; 21765), 1X penicillin/streptomycin and 5% FBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... prior to a one-hour incubation with SARS-CoV-2 N protein antibody (clone 1C7C7) at a 1:300 dilution and a AF594-conjugated Goat-anti-mouse secondary (ThermoFisher, A11005). Slides were counterstained with DAPI and scanned on an Akoya Polaris Vectra imaging system ...
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bioRxiv - Bioengineering 2023Quote: ... Two sets of 100 μl of either Protein A Magnetic Beads (Thermo PI88846) (rounds 1 and 2) or Dynabeads MyOne Streptavidin T1 (ThermoFisher Scientific) (round 3 ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: HEK293 cells were maintained at 37ºC and 5% CO2 in DMEM containing 10% FBS and 1% penicillin/streptomycin. The N protein plasmid pCAGGs-N (Plescia, David et al. 2021) was transfected using Lipofectamine™ 2000 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... or 4 μl of IgG control rabbit antibody at 1 mg/ml (SigmaAldrich I5006) were combined to 50 μl of protein A magnetic beads (Invitrogen 10001D) and added to sonicated chromatin (from 200 to 700 bp ...