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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... A 10 nM fluorescent bead solution was mixed with 250 µM of sulfo-NHS (N-hydroxysulfosuccinimide) (Thermo Scientific, 22980) and 250 µM of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride ...
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bioRxiv - Biochemistry 2024Quote: ... 100 ng dual-fluorescent reporter plasmids with various concentrations of PNAs were co-transfected into cells using lipo3000 (Invitrogen) according to the instructions ...
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bioRxiv - Microbiology 2024Quote: ... Multi-channel calibration was performed prior data acquisition using fluorescent TetraSpeckTM microspheres 0.1μm in diameter (Life-technologies, Molecular Probes). Stained cells were placed in Glox-MEA imaging buffer containing 50mM MEA ...
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bioRxiv - Microbiology 2024Quote: ... and cells were stained for 15 min with 5 μM green fluorescent nucleic acid stain SYTO™ 9 (Invitrogen) at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... and clones expressing the green fluorescent protein (GFP) were evidenced using a Safe ImagerTM 2.0 blue-light transilluminator (Invitrogen). Positive clones were selected and re-streak in LBKm plates ...
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bioRxiv - Developmental Biology 2024Quote: ... Different concentrations of acrylamide and bis-acrylamide were mixed to produce gels of different rigidity53 and mixed with 0.04% v/v fluorescent carboxylated 200 nm beads (Invitrogen), 0.05% APS (Sigma-Aldrich A3678) ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in a 12-well plate or 6-well plate using Lipofectamine 3000 (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The fluorgenic substrate (5 μM) was mixed with control and test samples in 96 well plates (Black wall plate, Nunc), incubated for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2019Quote: ... The cells were heat shocked in 50 μL (OD600 = 0.04) and transferred to plates (Nunc 96-Well Optical-Bottom Plate) containing 150 μL of their respective medium (final of OD600 = 0.01) ...
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bioRxiv - Microbiology 2020Quote: ... 10μl of lysate from the cell culture plate mixed then was transferred to a white Nunc 96-microwell plate (ThermoFisher) and 100μl of luciferase assay reagent (Promega Kit ...
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bioRxiv - Biochemistry 2022Quote: ... GTPase assays were performed in 200µl volumes in 96-well plates and the release of inorganic phosphate (Pi) was monitored at 360nm using the MultiSkan Go plate reader (ThermoFisher). Assays were performed in triplicates and a reference data set containing reaction buffer substrate (MESG ...
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bioRxiv - Immunology 2022Quote: ... the plates were read at multiple wavelengths (450 nm and 550 nm) on a plate reader (Multiskan GO, Thermo Fisher). The raw data were processed by Prism 9 (GraphPad ...
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bioRxiv - Bioengineering 2019Quote: ... the cells sampled at the same time points and diluted in PBS had their OD and YFP fluorescence (488nm excitation and 527nm emission) measured on a SynergyMX microtiter plate reader using clear-bottomed black microtiter plates (Thermofisher), and fluorescence values normalized to sample OD following the subtraction of background fluorescence from CEN.PK 113-7D ...
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bioRxiv - Synthetic Biology 2020Quote: Transformants were selected by plating first on YPD plates and then replica plating to yeast agar plates including 600 mg/L geneticin (Gibco) and incubating for a further 16 hours.
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bioRxiv - Biochemistry 2021Quote: 24.5 μL of 5 μM solution of every protein was prepared in 96-well plate MicroAmp Optical plate (Applied biosystems), and incubated at room temp ...
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bioRxiv - Immunology 2021Quote: Panning was performed against monomeric NTD directly absorbed to polystyrene plates (Maxisorb 96-well microtiter plates; Nunc, Sigma-Aldrich, USA). Blocking of plates and phages was conducted for 60 min using two blocking solutions ...
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bioRxiv - Immunology 2020Quote: ... braziliensis (MHOM/BR/1975/M2903) soluble antigen (2 μg/well) were adsorbed in microtiter plate (Nunc-immuno Plate, Roskilde, Denmark). Then ...
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bioRxiv - Developmental Biology 2022Quote: ... OPCs were seeded at 40,000 cells per well in 96-well plates or 50 million cells per 500 cm2 bioassay plate (166508, Thermo Fisher) (for generating large quantities of cells for sequencing studies ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL RNA + 95 μL H2O) into a new RNA dilution plate (96-well PCR quality plate – Thermo Scientific #AB0900). The final in-well concentration of the diluted RNA was in the range of 0.2 to 5 ng/μL.
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bioRxiv - Neuroscience 2022Quote: ... col seeded NGM plates and then moved to OP50 seeded plates containing 10 µM ThermoFisher scientific LysoTracker Blue DND-22 (Invitrogen) (1mM stock diluted 1:100 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 µL of culture with 26’000 cells was transferred in each well of the plate Nunc Edge 96-well plate (Thermo Scientific). The transfection mixture was added to the cells once it has incubated for approximately 30 min.
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bioRxiv - Cell Biology 2024Quote: HeLa cells were seeded at a density of 15,000 cell/cm2 in either 24-well plates or 12-well plates on #1.5 glass coverslips (Thermo Fisher Scientific). The following day ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fifty hydras were individually maintained at 18°C in cell culture plates (12-well plates, 1.5 mL/well, Thermo Scientific) filled with Volvic© water under a photoperiod of 12h ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Two microliters of each CFPS reaction were diluted with 33 μL of phosphate-buffered saline and analyzed in triplicate on a black 384-well plate with a multimode plate reader Varioskan Lux (ThermoFisher) at 488 nm excitation and 535 nm emission.
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bioRxiv - Molecular Biology 2023Quote: ... from one full NGM agar plate or from one well from 6 well plates and total RNA was isolated with Trizol (Invitrogen). DNAse treatment was achieved using DNA-freeTM ...
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bioRxiv - Cell Biology 2023Quote: ... In a next step 25µl of the compound cell culture media mix were carefully transferred from the intermediate plate on plates with the cells using a Beckman Coulter Biomek Fx followed by incubation in the Cytomat incubator (ThermoFisher) at 37°C and 5% CO2 for 24 and or 72 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and the supernatants were transferred to a UHPLC-adapted 96-well plate (Nunc 96-well V-bottom polypropylene plate, ThermoFisher) and sealed ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5 μM sgRNA stock plates were thawed and 5.76 μl of each guide was transferred to a 96 well conical bottom plate (Nunc, 249935) using a CyBio SELMA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and the protein concentration was determined using the Pierce™ BCA Protein Assay Kit following the manufacturer’s instructions and a Varioskan Lux plate reader (Thermo Fisher Scientific, Waltham, MA, USA). Samples were separated using the Mini-PROTEAN® TGX™ Precast Gel 4-15% (Bio–Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Products from the second PCR were purified as described above and concentrations were normalized using SequalPrep Normalization Plate (96) Kit (Thermo Fisher Scientific, Waltham, MA, USA). We pooled and concentrated the libraries using DNA Clean and Concentrator-5 Kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was conducted in 384 well plates using SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience, Cincinnati, OH, USA) in a QuantStudio 5 qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program ran according to enzyme requirements for 40 cycles ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was conducted in 384-well plates using the SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience) in a QuantStudio 5 (384-well plate) qRT-PCR cycler (Applied Biosystems, Foster City, CA, USA). The qRT-PCR program ran according to enzyme requirements for 40 cycles ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates for 60 minutes followed by TMB substrate (Thermo Fisher Scientific). Upon stopping the reaction with 1 M H2SO4 ...
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bioRxiv - Microbiology 2019Quote: ... using a Micro Amp Fast Optical 48-well reaction plate with 15 μ L reaction volume containing 1 x Power Sybr Green PCR Master Mix (Thermo Fisher), 0.2 μM of each primer and 1.5 μg of cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... Isolated PBMCs were cultured at a density of 5×105 cells/1 ml CM on 24-well plates coated with Geltrex (Thermo Fisher Scientific). After 24 h of incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:9 ratio (in 6-well plates at 60-70% confluency) using Lipofectamine®LTX with Plus™ Reagent (Invitrogen) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: MONHAs were seeded at 1 × 104 cells/cm2 on sterile glass coverslips in 24-well culture plates (Thermo Fisher Scientific, Waltham, MA). After 48 h ...
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bioRxiv - Cell Biology 2019Quote: ... HACAT and KB 3-1 cells were seeded into white 1536-well plates using a Multidrop Combi peristaltic dispenser (ThermoFisher, Waltham, MA) at a density of 250 ...
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bioRxiv - Immunology 2019Quote: ... Embryo carcasses were placed into individual 6 cm2 tissue culture plates containing 1 mL of pre-warmed 37°C TrypLE (Thermo Fisher, 12604013) and were incubated at 37°C for 20 min ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... 6.104 HL-1 cells were plated into each well of a 6-well plate and transduced overnight in 1 mL of transduction medium (OptiMEM-GlutaMAX, Life Technologies SAS) containing 5 µg/mL protamine sulfate in the presence of lentivectors (MOI 2) ...
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A key interfacial residue identified with in-cell structure characterization of a class A GPCR dimerbioRxiv - Biochemistry 2019Quote: ... and pcDNA3.1/HA-GPR17 (50 ng) or the mutant GPR17 plasmids (50 ng) per well in 96-well white plate (Thermo Scientific, Denmark). One day after transfection ...
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bioRxiv - Biophysics 2021Quote: Kinetic measurements of human erthyrocyte lysis (1%, v/v in PBS) by all ClyA mutants (at 10μM) was performed using a plate reader (Thermo Fisher - Verioscan reader) at 37◦C in shaking mode (120 RPM) ...
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bioRxiv - Neuroscience 2020Quote: ... The day before transfection, 1× 106 HEK293T cells were seeded in 10cm plate (DMEM medium supplemented with FBS and L-Glutamine, (Gibco, Ottawa, ON)) ...
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bioRxiv - Microbiology 2021Quote: The processed splenocytes were distributed at 1 x 106 per well into Nunc 96-well round bottom microwell plates (Thermo Scientific, UK). The cells were blocked by adding 5µg/ml purified rat anti-mouse CD16/CD32 (mouse BD Fc Block ...
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bioRxiv - Neuroscience 2020Quote: ... and placed in a 6-well plate filled with 1 ml/well of culture media containing 50 % Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23 % EBSS ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...