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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for 6 CHLORO 4 METHYL 3 PHENYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... or equimolar mix of two human APOBEC3A siRNAs (Silencer 45715 and 45810, respectively, with sense sequences 5’-GACCUACCUGUGCUACGAATT-3’ and 5’-GCAGUAUGCUCCCGAUCAATT-3’, Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) ...
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bioRxiv - Physiology 2021Quote: ... RNA targeting exon 3 of Ctns (gRNA-ex3 5’-ATCTTTCCAGAATCAACCGTCGG-3’) was produced using the Precision gRNA Synthesis Kit (Thermo Scientific). Online tools RGEN (http://www.rgenome.net/cas-designer/ ...
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bioRxiv - Neuroscience 2021Quote: ... or with a pool of two different USP30 siRNAs (D1: 5’-CAAAUUACCTGCCGCACAA-3’; D3, 5’-ACAGGAUGCUCACGAAUUA-3’, Dharmacon; siUSP30) by using Lipofectamine RNAiMAX (Thermo Scientific) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... or si-hsa-CHST15_0003 (n□=□3) or si-hsa-TNFRSF21_0001 (n□=□3) for 24□hours was carried out by using human Clariom™ Sassay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Developmental Biology 2021Quote: ... and human Oct 4 (For: 5’-CTTGCTGCAGAAGTGGGTGGAGGAA-3’/Rev: 5’-CTGCAGTGTGGGTTTCGGGCA-3’) PCRs were conducted using an ABI 7300 Real Time PCR System (Applied Biosystems). PCR cycling conditions were 95° C for 10 min. ...
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bioRxiv - Cancer Biology 2021Quote: ... The synthesized cDNA was amplified by EBNA 3C (1F and 1R) primers (5’-GAGAAGGGGAGC GTGTGTTGT-3’, 5’-GCTCGTTTTTGACGTCGGC-3’) by using regular PCR and adding Taq DNA polymerase (ThermoFisher, USA). The components of 25 μL reaction mixture contained 10 μL extracted DNA ...
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bioRxiv - Immunology 2021Quote: ... against the IAV nucleoprotein (forward: 5’-CAGCCTAATCAGACCAAATG-3’; reverse: 5’-TACCTGCTTCTCAGTTCAAG-3’) were assayed using SYBR Green Master Mix (Applied Biosystems) to confirm viral presence in the maternal lung.
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked for 3 h at room temperature in PBS with 3% powdered milk and 0.05% Tween 20 (Thermo Fisher Scientific). Protein detection was performed using the anti-EBOV GP (ZGP 12/1.1 ...
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bioRxiv - Genetics 2020Quote: ... mouse cDNA was amplified with primers (F: 5-gtttatgggcctcaacctcatg-3, R: 5-caggcttcactccagctttttgg-3) and then enzyme digested with BsiEI (Thermofisher #FD0894). Genotyping result using this method is shown in Fig ...
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were first loaded for 3 min onto the Acclaim PepMap 100 C18 trap column (75 µm x 2 cm, 3 µm material, Thermo Scientific) with 5 µL min-1 of 3.2% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were sonicated for 10 min in 100% HPLC EtOH (J.T baker 8462-25) and then incubated for 24 min in a mixture of 3% 3-Aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC EtOH ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates containing 60ug of total protein were electrophoresed for 3 hrs at 40C and 100 V using 3%-12% Bis-Tris polyacrylamide gels (ThermoFisher, BN1001BOX) and a running buffer containing 15mM Bis-Tris ...
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bioRxiv - Immunology 2023Quote: ... and 500 nM each of the forward primer and reverse primer (Table 3) with the following cycling conditions on either QuantStudio 3 or 5 Real-Time PCR systems (Applied Biosystems): 95°C for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... King’s B (KB) medium was prepared by mixing 20 g/L of proteose peptone #3 (BactoTM Proteose Peptone No. 3, ThermoFisher Scientific), 1.5 g/L of dipotassium phosphate (Carl Roth GmbH + Co ...
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bioRxiv - Microbiology 2023Quote: ... cells were blocked for 1 h in 3% BSA/PBS and incubated with rabbit-anti-GFP (1:50 in 3%BSA/PBS, 200 µl/well, Invitrogen, G10362) for 1 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... viable CD45-CD34+Sca-I- cells were sorted from epidermal single cell suspensions of 1-month-old EGFRΔEgr2 (n=3) and littermate controls (n=3) into Trizol LS Reagent (Thermo Fisher Scientific) using a FACS Aria III ...
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bioRxiv - Molecular Biology 2023Quote: ... HuH6 hCLDN1 [13] and Lunet N#3 and Lunet N#3 hCD81 [18] were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Thermo Fischer Scientific) supplemented with 1 % MEM non-essential amino acids (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and 643 bp downstream of 943rd nucleotide from 3’ end (3’ flank) of cysK2 were PCR amplified from Rv genomic DNA using Phusion DNA polymerase (Thermo Scientific). The amplicons were digested with BstAPI and ligated with compatible hygromycin resistance (hygr ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis (cleaved Caspase 3/7) measurements were done using the FMK-FLICA-Caspase 3/7 Kit (+ PI staining; Thermo Fisher Scientific) following the manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2 mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: RNAs were extracted from whole testes of 3 Ptbp1fl/fl and 3 Ptbp1ΔSC mice at postnatal day (P) 35 using the PureLink RNA Mini Kit (Ambion, Cat. 12183025). RNA-seq libraries were constructed using Illumina’s TruSeq Stranded mRNA sample prep kit ...
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bioRxiv - Cell Biology 2024Quote: ... GAPDH-F (5’-GGA GCGAGATCCCTCCAAAAT-3’) and GAPDH-R (5’-GGCTGTTGTCATACTTCTCATGG-3’) using Power SYBR Green PCR Master Mix (Applied Biosystems). All reactions were carried out on the StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... grown to confluence and incubated with the calcium indicator Fluo 4-AM (Fluo-4 Direct assay kit, Invitrogen) for 60 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... external cysteine residues were blocked with 107 μM 4-acetamino-4’-maleimidylstilbene-2,2’-disulfonic acid (AMS, Thermo Scientific) for 20 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4) for 10mins and later incubated with 4 uM fluo-4/AM (1 mM, Molecular probes, #F14201) and 0.002% pluronic F-127 (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... or separating the pooled samples on a 4% agarose gel (E-Gel® EX Agarose Gels, 4%, Invitrogen), and gel purification of fragments larger than adapter dimers (>150bp) ...
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bioRxiv - Neuroscience 2021Quote: ... Brain halves were either drop-fixed in phosphate buffered 4% paraformaldehyde 4°C (Thermo Fisher Scientific, Waltham, MA) for 24 hours at for immunohistochemical staining or micro-dissected (cortex ...
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bioRxiv - Immunology 2024Quote: ... The tissues were fixed overnight at 4 °C in 4% methanol-free paraformaldehyde (ThermoFisher Scientific, cat no. U01H501), and then washed three times with 1xPBS for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μl of 100 μl of sample was loaded onto 4-12% Novex Tris-glycine gel (Invitrogen, XP04120BOX). Western blotting was done using an anti-FLAG antibody ...
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bioRxiv - Bioengineering 2023Quote: ... Fluo-4 reagent solution (prepared according to manufacturer’s instructions; Fluo-4 Direct Calcium-Assay-Kit, Thermo Fisher Scientific) was added to culture medium (50% of culture medium volume ...
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bioRxiv - Neuroscience 2023Quote: ... heated for 2min at 85C and loaded on a TrisGlycine 4-20% SDS-PAGE gel (4-20%, Invitrogen) along with a PrecisionPlus Kaleidoscope protein ladder (BioRad 1610375) ...
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bioRxiv - Cell Biology 2024Quote: ... After fixing for 1h at 4°C in 4% PFA (prepared in PBS; Thermo Fisher Scientific, catalog 043368.9M), embryos were rinsed with PBS and then cryoprotected in 30% sucrose in 0.1M phosphate buffer overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 0.08 ml acetonitrile (Thermo Scientific, #A998-4), and 0.02 ml 2,4-dinitrophenylhydrazone (Beijingshiji ...
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bioRxiv - Immunology 2021Quote: ... or 4-12% Tris Bis gel (Invitrogen), transferred onto PVDF membrane ...
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bioRxiv - Developmental Biology 2021Quote: ... with a CytoSpin 4 (Thermo Fisher Scientific) at 1,000 rpm for 5 minutes followed by permeabilization with ice-cold methanol for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 4% heat-treated foetal bovine serum (Gibco), 1x non-essential amino acids (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: Fluo-4 Calcium Imaging Kit (Invitrogen F10489) was used for cytosolic Ca2+ measurement ...
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bioRxiv - Biochemistry 2020Quote: ... NativePAGE™ 4-16 % Bis-Tris (Invitrogen), according to the manufacturer’s instruction using Native PAGE™ 1X running buffer as anode buffer and NativePAGE™ 1X running buffer supplemented with NativePAGE™ 1X cathode buffer additive as cathode buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 4 mM glutamine (ThermoFisher Scientific), 1 mM pyruvate (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4 μL 0.1 M DTT (Thermo Fisher) and 2 μL SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... and quantified by Qubit 4 (Thermo scientific) for validating a concentration higher than 5 μg/μl.
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bioRxiv - Microbiology 2021Quote: ... 4 mM ATP (Thermo Fisher Scientific #R1441), and either 4 mM CaCl2 (to activate the kinase ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 uL of 10X DTT (Invitrogen something), 2 uL of SUPERase-In ...
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bioRxiv - Neuroscience 2020Quote: ... and FM™ 4-64FX (Invitrogen, F34653) to stain membranes ...
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bioRxiv - Microbiology 2022Quote: ... 4 mM L-glutamine (Thermo Fisher Scientific), 1 mM sodium pyruvate (Capricorn Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... and blasticidin (4 μg/mL; ThermoFisher Scientific) or geneticin (300 μg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4-20% Tris-Glycine gels (Invitrogen) followed by transfer onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... and fixed with 4% paraformaldehyde (PFA) (Affymetrix) for 20 min at room temperature (RT) ...