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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for 2 4 Difluoro N 2 methoxy 5 4 4 morpholinyl 6 quinazolinyl 3 pyridinyl benzenesulfonamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... were incubated with 10 µM red fluorescent Lipophilic Tracer DiD (1,1’-dioctadecyl-3, 3, 39, 39-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt; Thermo Fisher Scientific, Waltham, MA, USA) and/or 2 mM SYTO RNA-Select Green Fluorescent Cell Stain Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: The panel of synthetic peptide standards and the products of immunoprecipitated heparan-sulfate 6-O-sulfotransferase 1/2/3 (H6ST-1/2/3) in vitro Tyr sulfation assays were analyzed using Proteome Discoverer 2.4 (Thermo Scientific) in conjunction with MASCOT 59 against either a custom database of all (12 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 4 × 5 min with PBS before incubating with secondary antibodies (1:1000 Alexa fluorophores, ThermoFisher) in blocking solution for 1 hr at room temperature ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Infected cell pellets were centrifuged at 5000xg for 5 min at 4 °C and resuspended in Trizol (Ambion).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then stained with 5 µL FITC anti-human DR4 (Thermo Fisher Scientific, Clone DR-4-02) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... infected Jurkat cells were initially preloaded with 5 µM of CellTracker CMAC (7-amino-4-chloromethylcoumarin) (Life Technologies) and cocultured for 6 or 24 h with MDMs plated onto coverslips ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... clone G-A-5) followed by a secondary antibody solution (4 h, RT, AlexaFluor 594, 1:500, Invitrogen), mounted and coverslipped ...
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bioRxiv - Neuroscience 2021Quote: ... the washing medium was removed and 80 L of 5 M cell permeant Fluo-4 AM (Life technologies) in neurobasal medium was added to wells at room temperature and incubated at 37 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM DTT and 20% glycerol) at 30 °C in a Cytation 5 imaging reader (Thermo Fisher Scientific) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10µg of protein per sample was run on a 4%–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 minutes) at 4 °C and re-suspended in media composed of Minimal Essential Medium (MEM, Life Technologies) with 10%/vol fetal bovine serum (Omega) ...
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bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 60 min at 170V ...
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bioRxiv - Microbiology 2023Quote: ... 4 g/L dextrose, 5.957 g/L HEPES, 0.05 g/L hypoxanthine, 5 g/L Albumax II, Invitrogen) supplemented with 200 mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 5 min at 95°C and loaded onto a NuPAGE 4-12% Tris/Bis gel (ThermoFisher) with NuPAGE MOPS running buffer (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: Protein samples (5 µg protein) were mixed with either 4 x LDS sample buffer (Thermo-Fisher Life Technologies) or 4x LDS sample buffer + 50 mM dithiothreitol for SDS-PAGE analysis under non-reducing or reducing conditions ...
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bioRxiv - Genomics 2023Quote: ... then heated at 100°C for 5 minutes and loaded onto a 4-12% polyacrylamide gel (ThermoFisher NP0322). Transfer onto a PVDF membrane was performed in 1X NuPage Transfer Buffer (ThermoFisher NP00061 ...
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bioRxiv - Immunology 2022Quote: ... The cells were centrifuged at 400 g for 5 min at 4°C and resuspended in DPBS (GIBCO) supplemented with 5% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... 5–10mg of protein per sample was run on a 4–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 4 times before staining for 5 minutes with Pierce ECL Western Blotting Substrate (Thermo Fisher) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... cells were resuspended in 100 µL of PBS containing 5 µg/mL FM™ 4-64 (Molecular Probes). After incubation for 5 min at room temperature in the dark ...
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bioRxiv - Microbiology 2024Quote: ... cells were resuspended in 100 µL of PBS containing 5 µg/mL FM™ 4-64 (Molecular Probes). After incubation for 5 min at room temperature in the dark ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were incubated with 5 µM Fluo-4 AM and 0.02% Pluronic F-127 (Thermo Fisher Scientific, P3000MP) in a buffer solution for 30 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Cells were spun at 500G at 4°C for 5 minutes and resuspended in RNAlater ICE (Invitrogen AM7030).
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bioRxiv - Microbiology 2024Quote: ... Gametocyte cultures were seeded from asexual blood stage cultures at 2% parasitaemia and 4% haematocrit in gametocyte medium (RPMI-1640 with 5% human AB+ serum, 0.5% Albumax, 3.7% 100xHT supplement (ThermoFisher), 30 mg/ml L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in 400µL of freeze buffer (50 mM Tris-HCl pH 8.0, 40% glycerol, 5 mM MgCl2, 0.5 mM DTT, 4 u/mL RNase inhibitor [SUPERaseIN, Invitrogen]) and stored at -80°C.
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bioRxiv - Genetics 2020Quote: ... Total RNA was extracted from the left side of thorax with gynomorph-like color where siRNA was introduced and from the control region on the right side of the thorax of the same individual (N = 4 each for male and andromorphic female adults) using RNAqueous™-Micro Total RNA Isolation Kit (Thermo Fisher). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblotting of MSD2-mCherry was performed after native-PAGE or SDS-PAGE (12.5% separating gel and 4% stacking gel) with anti-mCherry primary antibody (Invitrogen, cat. n. M11217) diluted to 1:5,000 in Tris-buffered saline with 0.1% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... linker oligos with a 5’-terminal amine modification were obtained from IDT (Coralville, Iowa) and activated using sulfo-SMCC (Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) reagent (Thermo Fisher, #A39268) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the Sensation Plus FFPE Amplification and WT Labeling Kit (P/N 703089, Rev.4 Thermo Fisher Scientific Inc., Life Technologies). GeneChip ST Arrays (GeneChip Human Gene 2.1 ST Array Plate ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length genes of human RNGTT and CMTR1 were cloned into pMlink vector containing a 4×ProteinA tag at the N-terminal and were transfected to Expi293F cells (Thermo Fisher Scientific) when cell density is 2.5× 106 /ml ...
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bioRxiv - Bioengineering 2024Quote: ... which in turns intercalates with nucleic acids and emits red fluorescence detectable by microscopy.38 Frozen tissue sections from ATA samples (n=4 per group) were stained with DHE (10µM; D1168, Invitrogen; Carlsbad, CA, USA) in a dark humidified incubator at 37◦C for 20 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... L−1 of 3’-biotin-labeled primer probe purified by high-pressure liquid chromatography (HPLC) for 4-12 h at 42°C in Hybaid Shake ‘n’ Stack (Thermo Fisher Scientific) with gentle rotation ...
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bioRxiv - Physiology 2024Quote: Pancreatic islets were isolated from 13-14 week old female Rreb1+/+ and Rreb1+/- mice (n = 4 mice per genotype) and cultured overnight in RPMI-1640 GlutaMax™ (Gibco, 61870036) containing 10% FBS and 1% Pen-Strep ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... anti-desmocollin 2 and 3 (clone 7G6, Thermofisher), 1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... HSS117872 (Epsin-2) and HSS147867 (Epsin-3) (Invitrogen) on day 2 and 3 (Boucrot et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Passaged every 2-3 days with TrypleE (Gibco).
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bioRxiv - Genetics 2021Quote: ... the 700 bp PCR products were subcloned into the pCR™4-TOPO® TA vector and transfected into Escherichia coli DH5α cells (TOPO 4 Kit, ThermoFisher Scientific) followed by plasmid preparation and direct Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... pre-stained cells were loaded with Fluo-4 via by mixing with equal volumes of 2X Fluo-4 Direct™ loading solution (Fluo-4 Direct™ Calcium Assay Kit, Thermo Fisher). After a 30 min incubation at 37°C and then 30 mins at RT ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Microbiology 2020Quote: ... 4 and 24 hours after treatment and the plasma membrane was stained with FM 4-64 from Life Technologies (100 μg ml-1). Cells were observed with an epifluorescence Leica DM6000 B research microscope with a 100 x magnification.
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bioRxiv - Pathology 2023Quote: ... Sections were cut (4 µm) using a cryostat and labelled overnight at 4°C with COL6 antibody (1:500) (MA5-32412, Thermo Fisher Scientific, USA) after blocking nonspecific binding sites with 10% bovine serum albumin (A7906 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were quickly fixed overnight (ON) in 1x PBS pH7.4, 4% sucrose (Serva, Heidelberg, Germany) and 4% PFA (Paraformaldehyde; Thermo Fisher Scientific, Waltham, USA). Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, catalogue no. NW04122BOX) and transferred onto a polyvinyl difluoride membrane (Immobilon-FL ...