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Citations for Thermo Fisher :
3701 - 3750 of 5129 citations for TAGAP Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and then incubated with the secondary antibodies donkey anti-goat IgG Alexa Fluor™ 647 (1:500 in PBS blocking solution, A-21447, Invitrogen) and donkey anti-rabbit IgG Alexa Fluor™ 488 (1:400 in PBS with 0.05% tween ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with one or more of the following secondary antibodies in blocking solution for 30 minutes (Donkey anti-rabbit Alexa 488 [A21206; Fisher Scientific] ...
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bioRxiv - Cell Biology 2024Quote: ... were added at a dilution of 1:500 in blocking solution (Aligent Antibody Diluent #S202230-2) containing 1:100 Phalloidin 594 (ThermoFisher #A12381) to cells and incubated at 4 °C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by SDS-PAGE and then transferred onto nitrocellulose membranes which were blocked with Protein-Free Blocking Buffer (PFBS; Thermo Scientific) for 2 hours at room temperature in agitation ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in blocking buffer (3X SSC supplemented with 2% BSA, 0.1% Triton X100 and 2U/ul RNAse inhibitor (RiboLock, Thermofisher, Cat# EO0384)) ...
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bioRxiv - Neuroscience 2024Quote: ... Then the sections were incubated with primary antibodies in blocking buffer overnight at 4°C: AT8 (Mouse, 1:100, Thermo Scientific), and Iba1 (Rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... Per sample 80 µl of Protein G DynabeadsTM were 2 times washed with EBC lysis buffer and blocked overnight at 4 °C under constant rotation with SEA BLOCK blocking buffer (Thermo Fisher). After overnight incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then blocked for one hour at room temperature with a blocking reagent 2% bovine serum albumin (BSA; Fisher Scientific) in PBS followed by staining with primary antibodies ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were incubated overnight in a blocking buffer and primary antibody solutions containing the following: mouse anti-IBA1 (Invitrogen, 1:250), rabbit anti-IBA1 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed 4 times with PBS and then incubated with secondary antibody (goat α-mouse AlexaFluor 488 diluted 1:100 in blocking solution, Invitrogen) in the presence of 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2024Quote: ... We first incubated whole pre-fixed tissue in 1ml blocking solution (ice-cold PBS solution with 10% (v/v) Heat Inactivated Sheep Serum (HISS) (ThermoFisher, 16070096) and 0.5% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... Liquid Chromatography (LC) separation of the peptides was performed on an Ultimate 3000 Dionex RSLC-nano UHPLC (Thermo Fisher Scientific), equilibrated with 95% solvent A and 5% solvent B (equilibration solution) ...
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bioRxiv - Systems Biology 2020Quote: ... Peptides were introduced using an EASY-Spray source at 2000 V to a Fusion Tribrid Orbitrap mass spectrometer (Thermo Scientific). The ion transfer tube temperature was set to 275 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 3000 ng of the mixture tryptic peptides were analyzed using an Ultimate3000 high-performance liquid chromatography system (Thermo Fisher Scientific) coupled on-line to a Q Exactive HF-x mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were digested overnight at 37°C with trypsin (Pierce #90058) and the next day peptides were labelled with the TMTpro16plex reagents according to manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Peptides were trapped on a 2 cm column (Pepmap 100, 3μM particle size, 100 Å pore size; ThermoFisher Scientific, 164946), and separated on a 25cm EASYspray analytical column (75μM ID ...
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bioRxiv - Cell Biology 2020Quote: ... Obtained peptides were separated on a Thermo Scientific™ EASY-nLC 1000 HPLC system (Thermo Fisher Scientific™, Odense, Denmark) using a 45 min gradient from 5-60% acetonitrile with 0.1% formic acid and directly sprayed via a nano-electrospray source in a quadrupole Orbitrap mass spectrometer (Q ExactiveTM ...
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The derlin Dfm1 promotes retrotranslocation of folded protein domains from the endoplasmic reticulumbioRxiv - Cell Biology 2020Quote: ... Peptides were loaded on a C18 PepMap100 pre-column (inner diameter 300μm×5mm, 3 μm C18 beads; Thermo Fisher Scientific) and separated on a 50 cm reversed-phase C18 column (inner diameter 75 μm ...
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bioRxiv - Genetics 2021Quote: ... https://www.lifetein.com) polyclonal antisera raised against the N-terminal peptide of CenH3 [66] and anti-Rabbit-Alexa Fluor 488 (ThermoFisher Scientific). KNL2 and CENP-C were detected in root nuclei as previously described [67] using custom made anti-AtKNL2 [37] or anti-AtCENP-C antibodies (http://www.eurogentec.com/ ...
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bioRxiv - Genetics 2021Quote: Peptides were resuspended in 0.1% TFA and then analyzed using an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) coupled online to an EASY-nLC 1200 system (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... lymph-node derived cells were mixed in a 1:2 ratio with OVA-peptide pulsed spleenocytes and cultured in RPMI 1640 culture medium (Gibco) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: Splenocytes were processed as described in the previous section and stimulated with RBD peptides for 5 hours at 37°C with protein transport inhibitor (Invitrogen) and anti-mouse CD107a-FITC antibody (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: The eluted peptides were sprayed into the mass spectrometer by means of an Easy-Spray source (Thermo Fisher Scientific Inc.). All m/z values of eluting peptide ions were measured in an Orbitrap mass analyser ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were loaded directly onto a 75 μm x 50 cm PepMap-C18 EASY-Spray LC Column (Thermo Fisher Scientific) and eluted at 250 nl/min using 0.1% formic acid (Solvent A ...
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bioRxiv - Microbiology 2021Quote: ... The application of a 2.2 kV distal voltage electrosprayed the eluting peptides directly into the Thermo Exploris480 mass spectrometer equipped with an EASY-Spray source (ThermoFisher). Mass spectrometer-scanning functions and HPLC gradients were controlled by the Xcalibur data system (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... 5mM for 10−6M peptide loaded targets and 0.5mM for non-loaded negative control) and ENV641 targets were labeled with CFSE (ThermoFisher Sci) 5mM for 10−6M peptide loaded targets and 0.5mM for non-loaded negative control ...
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Resurgent Na+ currents promote ultrafast spiking in projection neurons that drive fine motor controlbioRxiv - Neuroscience 2021Quote: Mouse β4-WT (KKLITFILKKTREK) and β4-Scr (KIKIRFKTKTLELK) peptides (as previously described in Grieco et al. 200551) were synthesized by Thermofisher scientific at 98% purity and diluted to 100 μm in internal solutions for CC and VC experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... of “QTARKSTGGKAPRK” related peptides (1xAcetyl [K10]; 1xGln->pyro-Glu [N-Term]; 1xmethylations [K5]) using Qual Browser (Thermo xcalibur 4.1.50, Thermo Fisher Scientific) with ±15ppm width.
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bioRxiv - Molecular Biology 2020Quote: ... Eluting peptides were ionized at a potential of +2 kVa into a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). Intact masses were measured from m/z 350-1400 at resolution 120.000 (at m/z 200 ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted peptides were directly detected with an ion trap mass spectrometer (QExactive HF; Thermo Fisher Scientific Inc., Waltham, MA, USA). For ionization ...
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bioRxiv - Neuroscience 2020Quote: ... Percolator False Discovery Rate was set to 1% and Peptide abundance values were normalized in Proteome Discover 2.2 (Thermo Scientific). The experiments were performed in two separate sets consisting of (1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluting peptides were analyzed on a QExactive Plus (Qme IP) or QExactive HF (Nhp2 IP) mass spectrometer (Thermo Fisher Scientific). Peptide precursor m/z measurements were carried out at 70000 (Qme IP ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were injected and eluted through a 50 cm EASY-Spray column PepMap RSLC C18 (Cat. ES803; Thermo Fisher Scientific) with a 135-min gradient at constant 300 nL/min kept at 40°C ...
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bioRxiv - Immunology 2022Quote: ... Reconstituted peptides were tested for endotoxin contamination using a Limulus Amebocyte Lysate Chromogenic Endotoxin Quantitation Kit (Thermo Scientific, UK #88282).
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bioRxiv - Immunology 2022Quote: ... next peptides were fractionated based on their molecular mass using ultra performance liquid chromatography (UltiMate-3000 system, Thermo Fisher Scientific) and finally desalted and acidified on a C-18 cartridge (3M ...
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bioRxiv - Genetics 2019Quote: ... LC-MS/MS data acquisition: Peptide samples were analyzed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray ion source and a nano-flow LC system (Easy-nLC 1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The combined filtrates were desalted on a high pH reversed-phase peptide fractionation column (Pierce™, Thermo Fisher Scientific, USA). Then the peptides were collected followed by vacuum drying ...
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bioRxiv - Neuroscience 2019Quote: ... The peptides were separated and analyzed on a Dionex Ultimate 3000 RSLCnano system coupled to a Q-Exactive (Thermo Fisher). Approximately 2μg peptide from each fraction was injected into an Acclaim peptide trap column (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... Tryptic peptides were measured by nLC-MS/MS (Ultimate 3000 coupled to a Q Exactive, Thermo Fisher Scientific, Waltham, USA) applying a 3 h method (∼140 min elution window) ...
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bioRxiv - Molecular Biology 2019Quote: ... The peptides were concentrated (on-line) by reverse phase chromatography using a 0.1 mm × 20 mm C18 RP precolumn (Thermo Scientific), and then separated using a 0.075mm x 250 mm C18 RP column (Thermo Scientific ...
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bioRxiv - Biophysics 2019Quote: DSP crosslinked peptides liberated by EDT fragmentation were identified by Sequest searches of MS3 scans using Protein Discoverer 1.4 (Thermo Scientific). The searches were performed using a Swiss Prot rat database downloaded in May 2015 containing 8078 entries supplemented with the sequences of hamster SUR1 ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was dissolved in 300 µl of 0.1% TFA solution in water and subjected to fractionation using High pH Reverse-Phase Peptide Fractionation Kit (Thermo scientific) to 8 fractions and dried on a speedvac.
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bioRxiv - Cell Biology 2019Quote: ... Approximately 150 μg of peptides for each sample was labeled with 200 μg of TMT-6plex reagent (Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Systems Biology 2019Quote: ... Liquid Chromatography (LC) separation of the peptides was performed on an Ultimate 3000 Dionex RSLC-nano UHPLC (Thermo Fisher Scientific), equilibrated with 95% solvent A and 5% solvent B (equilibration solution) ...
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bioRxiv - Immunology 2020Quote: ... The peptide samples derived from individual patients were then individually labelled using the TMT10plex mass tag kit (Thermo scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The antibody was purified by affinity chromatography using the peptide immobilized on an agarose resin via the Sulfo-Link chemistry according to the manufacturer’s instruction (Thermo Scientific). The antibodies were eluted sequentially with a 0.2 M glycine solution at pH 3 and pH 2.5 ...
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bioRxiv - Cancer Biology 2020Quote: A synthetic peptide labeled with heavy arginine (13C, 15N) was formulated for the MDSTEPPYSQK(me3)R (eEF1A1_K318) peptide (HeavyPeptide AQUA custom synthesis service, Life Technologies). Synthetic peptides were spiked into the WT and SETD2-KO lysates from the HKC and 786-O cells ...
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bioRxiv - Plant Biology 2020Quote: High pH reverse phase fractionation was performed using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific PN84868) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with a N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The recombinant baculoviruses were generated according to the manufacture’s instruction to infect Hi5 cells at a density of 2×106 cells/ml ...