Labshake search
Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for GCLC Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
-
bioRxiv - Microbiology 2019Quote: ... Purified cells were cultured in a tissue culture flask containing 20 mL RPMI 1640 supplemented with recombinant murine Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) (10 ng/mL) (BioSource, Invitrogen cytokines & signaling, Camarillo, CA). At day 3 of the culture ...
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
-
bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... were prepared from 10 to 11-day-old chicken embryos (SPF eggs, VALO, Cuxhaven, Germany) using recombinant trypsin (Tryple TM, Thermo Fisher Scientific, Planegg, Germany) and maintained in VP-SFM medium ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were replated onto glass coverslips at day 12 of the differentiation in N2/B27 medium supplemented with four recombinant growth factors at 25ng/ml (BDNF; ThermoFisher, NT3, NGF, GDNF; Peprotech). CHIR90221 was included for 4 further days ...
-
bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
-
bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
-
bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific, Cat no. PHG0024). At day 6 ...
-
bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were incubated with the fluorescent secondary antibodies (Alexa Fluor 568-conjugated anti-rabbit IgG, 1:500, Invitrogen, A11011; 488-conjugated anti-rabbit IgG, Invitrogen A21206) for 3 h at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... were detected by immunofluorescence assay after acetone fixation using a polyclonal rabbit anti-PUUN serum diluted 1:1000 in PBS followed by AlexaFluor488-conjugated donkey anti-rabbit secondary antibody (Thermo Scientific). Nuclei were stained using Hoechst 33420 diluted 1:5000 in PBS ...
-
bioRxiv - Immunology 2019Quote: ... cells were blocked with 1% BSA at room temperature and incubated with primary rabbit anti-occludin antibody and secondary FITC-conjugated goat anti-rabbit antibody (Invitrogen, USA), respectively ...
-
bioRxiv - Biochemistry 2019Quote: ... ECL reagent (32132) and antibodies anti-oligomer A11 rabbit polyclonal (AHB0052) and goat anti-rabbit HRP conjugated (A16110) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... pneumoniae TIGR4 was detected using 1:2 optimized rabbit type-4 antiserum and 1:100 anti-Rabbit-Alexa-Fluor-488 (Thermo Fisher). Binding of human FH (Merck Millipore ...
-
Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... NPC1-eBFP2 was detected using a rabbit anti-BFP antibody followed by a secondary anti-rabbit antibody-Alexa 405 fluorophore conjugate (Thermo Fisher). Cells were examined by immunofluorescence analysis as described under the method section ‘Immunofluorescence microscopy and immunostaining’.
-
bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor488-labeled donkey anti-rabbit (A-21206), Alexa Fluor568-647- and 680-labeled goat anti-rabbit antibodies (A-11036, A-21245, A-21109) (ThermoFisher Scientific) as well as DyLight800-conjugated goat anti-rabbit and anti-mouse antibodies (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... Single immunofluorescence labeling of recombinant condensin I complex was processed as above except with 1 µg/mL rabbit anti-mSMC4 antibody (Lee et al., 2011) and Alexa Fluor 568-conjugated anti-rabbit IgG (1:500; Life Technologies) as previously described (Kinoshita et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... Alexa Fluor 488 Goat anti-rabbit (A-11008) and Alexa Fluor 568 Goat anti-rabbit (A-11011) were purchased from Thermo Fisher Scientific while secondary antibodies Goat anti-mouse HRP (172-1011 ...
-
bioRxiv - Immunology 2020Quote: ... gondii PVM was stained with polyclonal rabbit anti-GRA7 primary antibody (gift from John Boothroyd, Stanford University) and Alexa Fluor 488 anti-rabbit IgG (Life Technologies) at 1:3000 dilution ...
-
bioRxiv - Microbiology 2021Quote: ... or rabbit TgALD1 antibody (1:200, kind gift from Dr. Kentaro Kato) and anti-rabbit HRP antibodies (Thermo Fisher, 1:10000) followed by development of signal with West Pico Plus Chemiluminescent Substrate (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2020Quote: The rabbit α-NOCT polyclonal antibody was produced in rabbits inoculated with MBP-NOCT (1-431) (Thermo Fisher custom antibody services). This MBP-NOCT antigen (1-431 ...
-
bioRxiv - Cell Biology 2020Quote: ... Coverslips were then washed three times with PBS and incubated with AlexaFluor488 goat anti-rabbit or AlexaFluor647 goat anti-rabbit and AlexaFluor568 phalloidin (1:200; Life Technologies) diluted 1:200 in PBS for 1 hour at 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: Primary antibodies were rabbit anti-eIF4E-5 (1:500; #4524, this work) or rabbit anti-FLAG (1:500; Invitrogen, PA1-984B), and secondary antibodies were Alexa Fluor 488-conjugated anti-rabbit (1:200 ...
-
bioRxiv - Microbiology 2021Quote: Viral N protein was detected using a rabbit anti-SARS-CoV N antibody [32] as a primary antibody with AlexaFluor 568 anti-rabbit IgG or anti-rabbit IgG-HRP (Thermo Fisher) as secondary antibodies together with DAPI to stain the nucleus by indirect immunofluorescence as described previously [33].
-
bioRxiv - Immunology 2020Quote: ... PBMCs were fixed at 48hpi and stained intracellularly using CHIKV E1-specific rabbit antibody (kindly gifted by dr. G. Pijlman from Wageningen University) and secondary chicken anti-rabbit AF647 (Life Technologies). Samples were measured on Aurora flow cytometer (Cytek®) ...
-
bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-IgG 1:500, rabbit anti-Dp 212 1:500 (Dimova et al, 2003) and Dynabeads protein G (Invitrogen # 10003D). Chromatin precipitate was eluted from Dynabeads with elution buffer 1 (10mM EDTA ...
-
bioRxiv - Neuroscience 2022Quote: ... we confirmed targeting of the optic-fiber-tetrode bundle to LC by colocalization of the electrolytic lesion with immunostaining against tyrosine hydroxylase (rabbit anti-TH, ab152, Millipore, followed by donkey anti-rabbit Alexa 488, Life Technologies, or goat anti-rabbit Alexa 594 ...
-
bioRxiv - Neuroscience 2022Quote: ... matched sections were labelled in parallel with rabbit anti-μOR (1:1000, immunoStar) followed by donkey anti-rabbit Alexa Fluor 647 (1:500, Thermo Fisher). Image data were acquired using a 20X objective and z-stack tile approach on a Zeiss LSM700 confocal microscope ...
-
bioRxiv - Neuroscience 2024Quote: ... A subset of sections was stained for calbindin+ cells (1⁰ rabbit anti-calbindin (Swant #CB38) 1:500 + 2⁰ anti-rabbit-633 (Invitrogen #A21071) 1:500) ...
-
bioRxiv - Neuroscience 2024Quote: ... 1:750, 226004, Synaptic Systems; rabbit anti-PV, 1:1000, ab11427, ITK; or rabbit anti-HDAC2, 3 µg/µL, AB_2533908, Thermo Fisher) in PBS-BT for 18 hours at RT ...
-
bioRxiv - Microbiology 2024Quote: ... respectively as described above followed by incubation with a goat anti-rabbit fluorescent secondary antibody (Invitrogen Goat anti-Rabbit Alexa Fluor Plus 568™, Cat# A-11036, 1:1000 or Invitrogen Goat anti-Rabbit Alexa Fluor™ Plus 647 ...
-
bioRxiv - Immunology 2023Quote: ... or with rabbit-anti-human TG3 (PA596450, Thermo Fischer Scientific) followed by detection with Alexa 488 donkey anti-rabbit (Molecular Probes) antibody ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The secondary antibodies were diluted to 1:250 (Goat anti-rabbit IgG Cy5; Invitrogen A10523; goat anti-mouse IgG AlexaFluor 488, Invitrogen A11029; goat anti-rabbit IgG AlexaFluor 488, Invitrogen A11034). Nuclei were stained with Hoechst 33258 (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... were selected and processed as described above with primary antibody Rabbit anti-Cox-2 (1:1k, Cayman Chem, 160123) and secondary antibody AF555 donkey anti-rabbit (1:200, ThermoFisher, A31572).
-
bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h ...
-
bioRxiv - Neuroscience 2024Quote: ... and then incubated for 2 h with Alexa Fluor 488 donkey anti-rabbit IgG or Alexa Fluor 568 donkey anti-rabbit (ThermoFisher Scientific).
-
bioRxiv - Neuroscience 2024Quote: ... visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock; Goat anti rabbit AF488, Thermo Fisher Scientific A11034, RRID:AB_2576217). Slides were imaged on an ANDOR Dragonfly microscope (Oxford Instruments ...
-
bioRxiv - Microbiology 2019Quote: ... followed by immunostaining with a rabbit polyclonal MUC2 antibody and goat anti-rabbit Alexa-Fluor 488 antibody (Life Technologies, Paisley, United Kingdom). Slides were imaged using a BX51 upright microscope and a Coolsnap EZ camera (OLYMPUS ...
-
bioRxiv - Microbiology 2019Quote: ... The SFTSV foci were detected using rabbit anti-SFTS NP antibody (Abclon) and goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (Thermo Fisher Scientific). Viral plaques were visualized by incubation with NBT/BCIP solution (Roche ...
-
bioRxiv - Neuroscience 2021Quote: ... Golgin-97 was stained with rabbit anti-GM97 (CST, #D8P2K) and visualized with goat anti-rabbit conjugated to Alexa Fluor 405 (Life Technologies, #P10994). HA-Rab29 was stained with mouse anti-HA (Abcam ...
-
bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated anti-rabbit (Cytiva, NA934V) or fluorescence-based (Cy3-conjugated anti-mouse or Cy5-conjugated anti-rabbit; Invitrogen, Thermo Fisher Scientific) secondaries were used at 1:1000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated anti-rabbit (Cytiva, NA934V) or fluorescence-based (Cy3-conjugated anti-mouse or Cy5-conjugated anti-rabbit; Invitrogen, Thermo Fisher Scientific) secondaries were used at 1:1000 ...
-
bioRxiv - Microbiology 2019Quote: ... Klebsiella was labelled with rabbit anti-Klebsiella serum (1:1000) and visualised with goat anti rabbit Alexa Fluor 350 life-tech (1:1000) (Molecular Probes, A11046). Fluorescence images were captured using the ×100 objective lens on a Leica DM5500 microscope equipped with the appropriate filter sets ...
-
bioRxiv - Bioengineering 2021Quote: ... The antibody probes used were primary rabbit-anti-tGFP antibody (Pierce PA5−22688,) and secondary polyclonal antibody AlexaFluor-647-labeled donkey-anti-rabbit (Invitrogen A-31573). Bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2021Quote: ... immunostained with rabbit anti-O78 antibody followed by cross absorbed goat anti-rabbit AlexaFluor 594-conjugated IgG (H+L) (ThermoFisher A-11072). Membranes with fluorescently labeled samples were excised and mounted onto glass slides with ProLong Gold (ThermoFisher Scientific P36930) ...