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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... at a density of 1.2-1.6 million cells/mL using 25 kDa linear polyethylenimine (PEI) at a 3:1 PEI:DNA ratio in OptiMEM reduced serum medium (Gibco), with a heavy chain ...
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bioRxiv - Genomics 2020Quote: 20-5 million of cells were washed twice with ice-cold PBS before adding swelling buffer (10 mM Tris-HCL pH 7.5, 2mM MgCl2, 3 mM CaCl2, 2U/ml Superase-in (Invitrogen)) ...
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bioRxiv - Microbiology 2019Quote: ... were added and cells were grown at 37°C with aeration for 30 minutes before 0.5 mL were pelleted at 5,000 × G for 3 minutes and resuspended in 0.1 mL of phosphate buffered saline (pH 7.2; Gibco Life Technologies) containing 20 μM 3,3’-diethloxacarbocyanine iodide (DiOC2(3) ...
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bioRxiv - Microbiology 2019Quote: ... were added and cells were grown at 37°C with aeration for 30 minutes before 0.5 mL were pelleted at 5,000 × G for 3 minutes and resuspended in 0.1 mL of phosphate buffered saline (pH 7.2; Gibco Life Technologies) containing 20 μM 3,3’-diethloxacarbocyanine iodide (DiOC2(3) ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 3 hours with 1 μg/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Physiology 2022Quote: ... single cell suspension of bone marrow cells was prepared by flushing the femurs and tibias using 3 ml ice-cold staining medium (Ca2+-and Mg2+-free Hank’s balanced salt solution, HBSS (Gibco) supplemented with 10 mM EDTA (Corning ...
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bioRxiv - Synthetic Biology 2024Quote: ... a solution of 10-3 mg/ml PE- conjugated anti-human CD62E (clone P2H3, Thermofisher Scientific, #12-0627-42) has been introduced in the chips to stain the endothelium for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... 10 mM NaCl, 3 mM MgCl2, 1 mM dithiothreitol, 1% BSA, 0.1% Tween 20, and 0.6 U/mL RiboLock [Thermo Fisher]). Next ...
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bioRxiv - Immunology 2024Quote: ... The lungs were washed by bronchoalveolar lavage with 3 mL of PBS and then digested in media (DMEM (Gibco), 2 mg mL−1 collagenase (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl pH 9 using a Slide-A-Lyzer 0.5 - 3 mL 10,000 MWCO dialysis cassette (Thermo Scientific). The conjugation reaction was performed overnight at 21 °C and 750 rpm with 2 equivalents of DBCO-SL8 or DBCO-PEG4-SL8 per STxB monomer and 10 % DMSO to keep the peptides soluble ...
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bioRxiv - Biochemistry 2023Quote: ... followed by an overnight dialysis step against PBS using Slide-A-Lyzer 0.5 - 3 mL 10,000 MWCO dialysis cassettes (Thermo Scientific). sSTxB(70AN3)-SL8 conjugates formation and the absence of remaining free SL8 after purification were validated by UPLC-MS ...
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bioRxiv - Biochemistry 2023Quote: ... The excess of free G15F peptide was removed by overnight dialysis against PBS using Slide-A-Lyzer 0.5 - 3 mL 20,000 MWCO dialysis cassettes (Thermo Scientific). The formation of sSTxB(70AN3)-G15F conjugates and the absence of remaining free G15F peptides after dialysis were validated by UPLC-MS ...
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bioRxiv - Microbiology 2023Quote: ... TREx BCBL1-Rta cells transduced with lentivirus were cultured in medium further supplemented with 3 μg/ml puromycin (ThermoFisher). KSHV lytic reactivation was induced in TREx BCBL1-Rta cells using 2 μg/ml doxycycline hyclate (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Ganglioside M1 (GM1) staining was performed using recombinant Choleratoxin B labelled with AlexaFluor647 (3 µg/ml, Thermo Fisher, #C34778). Both GFP-D4 and AlexaFluor647-labelled CTB were added to FACS buffer-washed cells and incubated for 20 minutes at 4°C in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice with ice-cold PBS before adding swelling buffer (10 mM Tris-HCL pH 7.5, 2mM MgCl2, 3 mM CaCl2, 2U/ml Superbase-in (Invitrogen)) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cycling was performed using a QuantStudioTM 3-96 well 0.1 mL Block Real time PCR Detection system (ThermoFisher, USA), and transcript quantities were derived by the system software ...
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bioRxiv - Microbiology 2023Quote: ... containing 3 mL of home-made Expansion Medium consisting of a 1:1 mixture of DMEM/F-12 (Gibco) and Neurobasal Medium (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 30 μl of the proteins at 3 μg/mL were plated onto 384-well Nunc Maxisorp plate (ThermoFisher, 464718) in 1x TBS and incubated 1h at 37°C followed by slap drying and blocking with 80 μL of Casein for 1 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 30 μl of the proteins at 3 μg/mL were plated onto 384-well Nunc Maxisorp plate (ThermoFisher, 464718) in 1x TBS and incubated 1h at 37°C followed by slap drying and blocking with 80 μL of Casein for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: To assess the barrier formation of each CRC organoid, 50 μg/mL Dextran (Cascade Blue, 3 kDa) (Invitrogen, #D7132) was added to the top channel ...
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bioRxiv - Developmental Biology 2023Quote: Eyes were removed and collected in a 35 mm culture dish containing 3 mL of M199 medium (11825015, Gibco) supplemented with 2 mM L-glutamine (25030081 ...
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bioRxiv - Biochemistry 2023Quote: ... the paper was air dried and transferred to scintillation vials filled with 3 mL of ScintiVerse cocktail (Thermo fisher). The radioactivity of tritium was measured with a Beckman LS6500 counter.
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; S911, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 1 µL mouse anti-V5 primary antibody (1:10,000; R96025, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were anesthetized with 0.3% MESAB prior to spinal injection of 10 mg/mL 10,000 MW Tetramethylrhodamine dextran (10,000 MW, D1866, Thermo Fisher) in PBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... Each tube was then sequentially dosed with 3 µL carrier DNA (ThermoFisher salmon sperm DNA, sheared, 10 mg/mL) and library plasmid stock (1 µg of plasmid per tube) ...
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bioRxiv - Immunology 2024Quote: ... Epidermal cell suspensions were prepared by scraping the dermis away with forceps and incubating the layer of epidermis on 3 mL of 0.5% Trypsin-EDTA (ThermoFisher) at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed for 5 min at room temperature with 1 % methanol-free formaldehyde (Thermo Scientific, #28906). Cells were lysed using ice-cold Farnham lab buffer supplemented with complete protease inhibitor cocktail (#4693159001 ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM EGTA) then simultaneously fixed and permeabilized in 1.8% formaldehyde (methanol-free, Thermo Fisher Scientific 28906) and 0.5% Triton-X100 in CSK for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mg/mL transferrin (MilliporeSigma, T8158), 5 ng/mL sodium selenite (MilliporeSigma, S5261) and 100 units/mL penicillin-streptomycin solution (Gibco, 15140122). Human ovarian cancer NIH:OVCAR3 (OVCAR3 ...
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bioRxiv - Synthetic Biology 2019Quote: ... × 5 mm 5 µm 100 Å and an Acclaim PepMap RSLC 75 µm × 25 cm 2 µm 100 Å (Thermo Scientific). The columns were installed on an Ultimate 3000 RSLCnano system (Dionex) ...
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bioRxiv - Cancer Biology 2021Quote: ... 800 ng of digests were loaded in random order onto a pre-column (C18 PepMap 100, 5 µm, 100 A, 300 µm i.d. x 5 mm length, Thermo Fisher, 160454) at a flow rate of 50 µL/min with solvent C (0.05% TFA in water/acetonitrile 98:2).
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bioRxiv - Biochemistry 2020Quote: ... Acclaim™ PepMap™ 100 C18 LC Column (5 mm x 0.3 mm i.d., 5 μm, 100 Å, Thermo Fisher Scientific) was used as trap column at a flow rate of 25 μL min-1 kept at 45 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The peptides were first trapped for 1 min at 30 μl/min with 100% buffer A on a trap (0.3 mm by 5 mm with PepMap C18, 5 μm, 100 Å; ThermoFisher Scientific); after trapping ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were then washed with 1mL of ice-cold lysis buffer four times before elution of bound material via a 5 min incubation at 98 °C with 30 μL of 2x LDS buffer (Invitrogen). About 25 μL of eluate was collected by centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.06% Bromophenol Blue) at 98°C for 5 minutes and separated on 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... HEKT cells were grown in a humidified 37°C incubator with 5% CO2 using media supplemented with 100 I.U./mL penicillin and 100mg/mL streptomycin (Invitrogen). The human cell lines HEK293T were maintained in DMEM (VWR ...
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bioRxiv - Microbiology 2022Quote: ... One ml of fd or Pf4 phage (5 mg/ml) was incubated with 100 µg A488 fluorescent dye (ThermoFisher) for 1 hour at room temperature (RT ...
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bioRxiv - Immunology 2024Quote: ... FITC-IgG+ and biotin-blocked Per-CP streptavidin negative cells from this gate was sorted into wells containing 3 µl chilled lysis buffer (0.5X Gibco PBS, 3 U/µl RNaseOUT and 10 mM DTT) for a subsequent reverse-transcriptase reaction in a 96 well PCR plate ...
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bioRxiv - Bioengineering 2020Quote: ... and incubated with 100 nM LysoTracker Red DND-99 (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... at 100 nM and LysoTracker Red DND-99 (Thermo Fisher) at 50 nM after 30 minutes of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Freshly dissected tissues were incubated for 5 min in 50 nM LysoTracker Red DND-99 (Life Technologies, Cat# L7528) in PBS ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ T-cells were stained with LysoTracker Red DND-99 (Invitrogen; 5 μM; 1 h; 37°C), fixed (3% PFA ...
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bioRxiv - Cancer Biology 2021Quote: ... ChIP-qPCR was performed using the SYBR Green qPCR Master Mix (SolomonBio, QST-100) on the QuantStudio 3 Real-Time PCR system (Applied Biosystems). Primers were listed in Supplementary Table S1.
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bioRxiv - Neuroscience 2020Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Scientific) equilibrated with 0.05% TFA ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were injected onto an Acclaim PepMap100 C18 trap column (75 μm i.d., 2 cm long, 3 μm, 100 Å, Thermo Scientific) and further separated on an Acclaim PepMap100 C18 analytical column (75 μm i.d. ...
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bioRxiv - Molecular Biology 2021Quote: ... peptides were trapped and separated on a 100 μm x 250 mm C18 column with 3 μm particle size PepMap Easy-Spray (Thermo Scientific) using a Dionex Ultimate 3000 nUPLC at 200nl/min ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were gradient eluted at 300 nL/minute from 3% to 20% acetonitrile over 100 min into an Orbitrap Fusion mass spectrometer (Thermo Scientific). Precursor mass spectra (MS1 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were dehydrated with ethanol series (70%, 95%, then 100%) and hybridized with TAMRA-OO-(TTAGGG)3 PNA probe (Applied Biosystems) in formamide hybridization solution (70% formamide ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...