Labshake search
Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for 7 Fluoro 4 hydroxy 3 nitroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and Qubit 4 Fluorometer (Thermo Fisher Scientific), respectively ...
-
bioRxiv - Biophysics 2023Quote: ... and 4 uL RNaseA (Thermo Scientific; EN0531) in RNaseH buffer using the following program ...
-
bioRxiv - Microbiology 2023Quote: ... 4 U of turbo DNase I (Ambion) was added (37°C ...
-
bioRxiv - Immunology 2024Quote: ... 96-well Immuno 4 plates (Fisher Scientific) were coated with 10mg/ml goat anti-mouse Ig (Southern Biotechnology ...
-
bioRxiv - Bioengineering 2023Quote: ... a 4% low melting point agarose (Invitrogen) solution was prepared in sterile 1X PBS and kept in a 37°C water bath ...
-
bioRxiv - Microbiology 2024Quote: ... cells were fixed in 4% paraformaldehyde (Invitrogen) for 30 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... 4% formaldehyde (Thermo Fisher, cat. BP531-500) was applied to cells for fixation for 30 min at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: PFA 4% (ThermoFisher, cat. no. J19943.K2) ! CAUTION Very toxic and carcinogenic substance that may cause death or damage to health when inhaled ...
-
bioRxiv - Biochemistry 2024Quote: ... 4–12% Bis-Tris gels (ThermoFisher, #NP0322) and run in 1X MOPS buffer (ThermoFisher ...
-
bioRxiv - Molecular Biology 2024Quote: ... using the Vitrobot Mark 4 (Thermo Fisher) at a relative humidity of 100% and 4 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... and 4% sucrose (Fisher Scientific, S5-500) for 30 minutes at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... CD152 (CTLA-4) (Invitrogen, APC, clone 14D3), HLA-DR (BD ...
-
bioRxiv - Neuroscience 2024Quote: ... with 4% fetal bovine serum (Gibco, 175004044) and the cortices were manually dissociated using a pipette ...
-
bioRxiv - Genomics 2024Quote: ... 4% B27 supplement (ThermoFisher Scientific, #17504-044), 30 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were fixed in 4% formaldehyde (ThermoFisher) for 15 minutes at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... eyes had 15µl of 4% formalin (ThermoFisher) dropped onto them and were left to fix at room temperature for 15 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... and 4 μg/mL laminin (Life Technologies).
-
bioRxiv - Cancer Biology 2021Quote: ... clone IMAGE ID 4977050 was obtained from Source Bioscience and PCR cloned using oligos (Tet2fwd: 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAatgccaaatggcagtacagt-3’ and Tet2rev: 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTTtcatacaaatgtgttgtaag-3’) into pDonor221 (Invitrogen Gateway, ThermoFisher) and sequenced ...
-
bioRxiv - Cell Biology 2020Quote: ... or a 1:1 mixture of two siRNA to CHMP2A (5’-CAGGCCGAGAUCAUGGACAUG-3’ and 5’-GAAGAUGAAGAGGAGAGUGAC-3’) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: MiaPaCa-2 cells were seeded in 12-well plates and transfected with 3 µg of DNA (NUPR1-GFP, NUPR1-Flag or control vector) and 3 μL of Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific) per well ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer containing the Sp6 promoter (5’ ATTTAGGTGACACTATAGCCTCTTCAGTTCCTTCTTCCATC-3’).The aqp1a.1 probe was generated from whole extracted cDNA at 30hpf and amplified with the primers (5’-GTCATGAACGAGCTGAAGAGC-3’) and (5’-GGGTCACTTTGAGGACATCTC-3’),incorporated into the pCR-BluntII-TOPO vector (Invitrogen, 45-0245) (containing both the SP6 and T7 promoters) ...
-
bioRxiv - Developmental Biology 2021Quote: ... were transfected with pPyCAG-(HA)3-Nanog-IRES-Puro and pPyCAG-(Flag)3-Resf1-IRES-Puro using Lipofectamine 3000 (Invitrogen cat. L3000001). In parallel ...
-
bioRxiv - Genomics 2020Quote: ... The 16S rRNA gene was amplified from the extracted genomic DNA using 16S universal primers 27F (5’-AGA GTTTGATCMTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTTC-3’) and sequenced using an automated ABI3730XL capillary DNA sequencer (Applied Biosystems, USA) for taxonomic identification at Cosmo Genetech (Seoul ...
-
bioRxiv - Neuroscience 2021Quote: All females were ovariectomized and primed for 3 days with subcutaneous administration of estradiol benzoate (17-β-Estradiol-3-Benzoate, Fisher Scientific, 2 μg dissolved in sesame oil starting 3 days prior to experiments) ...
-
bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
-
bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
-
bioRxiv - Microbiology 2020Quote: ... 229E-RP reverse primer (5’-CCAACACGGTTGTGACAGTGA-3’) and the TaqMan minor groove binder (MGB) probe 229E-TP (FAM-5’-TCCTGAGGTCAATGCA-3’-NFQ-MGB; Thermo Fisher Scientific), derived from the HCoV 229E membrane protein gene sequence as described previously (Vijgen et al. ...
-
bioRxiv - Microbiology 2021Quote: The mcr-1 gene was amplified from the clinical isolate ESBL20150072 (European Nucleotide Archive, accession number; SAMEA104060671) using primers: 5’-TTTTGGATCCCGGTTTTCGGGCTTTTTA-3’ and 5’-ATATGTCGACTCAGCGGATGAATGCGGT-3’ and Phusion polymerase (Thermo Fisher Scientific). PCR product was digested with BamHI and SalI and ligated into pACYC184 digested with the same enzymes (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
-
bioRxiv - Biochemistry 2022Quote: ... was mixed with 10 nm gold nanoparticles and was applied to the glow discharged surface and blotted at 4 °C and 100% relative humidity for 3 s and a blot force of −3 using the Vitrobot IV System (Thermo Fisher Scientific). Grids were plunged in 100% liquid ethane ...
-
bioRxiv - Genetics 2022Quote: ... 5’-GGCAAGCUGACCCUGAAGUdTdT-3’ / 5’-ACUUCAGGGUCAGCUUGCCdTdT-3’ or with a hsa-miR-34a mimic duplex: 5’-P-UGGCAGUGUCUUAGCUGGUUGUU-3’ / 5’-P-CAAUCAGCAAGUAUACUGCCCUA-3’ according to the protocol for Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2019Quote: ... and then induced to differentiate by culturing for further 3 days in a 3:1 mixture of DMEM and Ham’s F12 media (Invitrogen, Palo Alto, CA) containing FCS (10%) ...
-
bioRxiv - Plant Biology 2021Quote: ... a fragment containing 2.21 kb upstream regulatory region of ELF3 was amplified from Col-0 genomic DNA using primers ELF3Prom-Fw (5’-CACCCTTATAAATAAAATTCC-3’) ELF3Prom-Rv (5’-CACTCACAATTCACAACCTTTTTC-3’) and cloned into the Gateway System (pENTR™ Directional TOPO® Cloning kit, Invitrogen) to obtain the pELF3-TOPO plasmid ...
-
bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
-
bioRxiv - Plant Biology 2022Quote: 5’-biotinylated RNA and unmodified RNA of Motif 3 (5’-AAAUCGCCGGAG-3’ 5’-Bio-AAAUCGCCGGAG-3’) were synthesized by Eurofins (Hamburg, Germany) and used for LightShift™ Chemiluminescent RNA EMSA Kit (Thermo Scientific). Total protein was extracted from LL and 10 min LL➔HL-treated plants using extraction buffer (25 mM Tris ...
-
bioRxiv - Microbiology 2021Quote: ... the hypervariable V3 region of the 16S rDNA gene was amplified from 20 ng of DNA using the primers 5′-CCTACGGGAGGCAGCAG-3′ and 5′-ATTACCGCGGCTGCTGG-3′ (Integrated DNA Technologies BVBA, Leuven, Belgium),(18) 1U Platinum® PCR SuperMix High Fidelity (ThermoFisher Scientific) and 10 μM of primer-mix ...
-
bioRxiv - Cancer Biology 2019Quote: ... (5’-CUAUGAACAUAAAGUCUGCTT-3’) or its non-specific control (5’-UUCUCCGAACGUGUCACGUTT-3’) were transfected into cells using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA).
-
bioRxiv - Neuroscience 2021Quote: ... Drosophila dSF2 sequence was PCR amplified (dSF2 F 5’-CACCATGGGATCACGCAACGAGTGCCG-3’ and dSF2 R 5’- ATAGTTAGAACGTGAGCGAGACCTGG-3’) was cloned into pEntry-TOPO vector (Thermo Fisher Scientific). The pENTR-dSF2 vector was recombined with Gateway plasmid pTWH (Drosophila Genomics Resource Center ...
-
bioRxiv - Genetics 2021Quote: ... SDS-Page separation was completed by running between 3 and 10μg of total protein on a NuPAGE 3%–8% Tris-acetate gel (Thermo Fisher Scientific Scientific). Next ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
-
bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
-
bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
-
bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
-
bioRxiv - Microbiology 2022Quote: ... The 16S rRNA gene was amplified with the primers 8f (5′-AGAGTTTGATCCTGGCTCAG-3′; Weisburg et al. 1991) and 1520 r (5′-AAGGAGGTGATCCAGCCGCA-3′; Edwards et al. 1989) (Invitrogen, CA, USA). A volume of 0.5 μL DNA extract was used for 50 μL PCR reactions containing 2 units Taq DNA polymerase ...
-
bioRxiv - Molecular Biology 2023Quote: ... The following day the miR-423-5p or miR-Ctrl vector was cotransfected with either the Cacna2d2-3’UTR or the Cacna2d2 mut 3’UTR reporter vectors using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2024Quote: ... and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (BODIPY FL DHPE) were purchased as solids from Invitrogen (Thermo Fischer Scientific), Avanti Polar Lipids ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10 μl of sample was saved for 3′ -biotin labeling for visualization using Pierce RNA 3′ End Biotinylation Kit (Life Technologies 20160) according to manufacturer’s protocol ...
-
bioRxiv - Physiology 2024Quote: ... 2 µl cDNA was amplified with TaqMan™ qPCR (38 cycles, 3 technical replicates of 3 biological replicates) using master mix (Thermo Fisher) and the following TaqMan assays ...
-
bioRxiv - Immunology 2024Quote: ... and SB28 RRV-IRF8 (100% transduced) cells were plated at 1x105 cells (n=3 per time point) and counted (Thermofisher Countess 3)at 24 and 48 hours post-seeding ...
-
bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...