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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-tagged USP7 WT and mutants were then subcloned into the pcDNA5/FRT/TO vector (Invitrogen, Thermo Fisher Scientific) and sequence verified before generating inducible FlpIn T-Rex cell lines ...
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bioRxiv - Cell Biology 2021Quote: N-terminally MBP-tagged (pTHMT) FUS CHOP fusion type II and CHOP oncogene were expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7–0.9 before induction with 1 mM isopropyl-b-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no. A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Microbiology 2021Quote: ... A549 cells were transiently co-transfected with 100 ng in total of an N- or C-terminal LgBiT and SmBiT tagged construct using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher). All possible combinations of the N-terminal and C-terminal-tagged split luciferase protein pairs were tested ...
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bioRxiv - Biochemistry 2021Quote: ... For staining FLAG tagged receptors we used anti-FLAG M1 antibody (1:100) labelled with DyLight594 (Thermo Scientific, Cat. no. 46412) dye in presence of 2 mM CaCl2 ...
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bioRxiv - Neuroscience 2019Quote: pLVX-EF1a-TS-EGFP-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged EGFP cDNA (DNA String byGeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Neuroscience 2019Quote: ... pLVX-EF1a-TS-OPT-FUS-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged codon optimized FUS cDNA (Gene synthesis by GeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... along with 50 ng and 100 ng of expression plasmids encoding 3xHA-tagged wildtype or mutant mouse PORCN-D as indicated using Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... The tissues were counterstained with wheat germ agglutinin (WGA) tagged with Alexa Fluor™ 647 (#A-31573, ThermoFisher Scientific, Waltham, MA) and DAPI (#H-1500 ...
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bioRxiv - Developmental Biology 2020Quote: ... All MEGF8 constructs were tagged with a C-terminal 1D4 and cloned into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Invitrogen) or pLenti CMV PURO DEST (Addgene ...
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bioRxiv - Developmental Biology 2020Quote: ... All MEGF8 constructs were tagged with a C-terminal 1D4 and cloned into pEF5/FRT/V5-DEST (Thermo Fisher Scientific, Invitrogen) or pLenti CMV PURO DEST (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... cells were rinsed thrice with PBS and incubated with secondary antibody (donkey anti-goat IgG, tagged with Alexa Fluor 488, Thermo Fisher) at 1:500 dilution for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were transfected with 100ng EGFP to mark cell bodies and 50-100ng of GFP tagged TDP-43 constructs using Lipofectamine 2000 (Invitrogen 52887), as previously described[41 ...
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bioRxiv - Biochemistry 2021Quote: Approximately 20 micrograms of purified 6xHis-tagged KRAS4BA59T were reduced with 5 mM tris(2-carboxyethyl)phosphine (ThermoFisher Scientific, Cat #77720) for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... RRV cDNA was generated from 10 μl of serum derived RNA or 1 μg of tissue derived RNA using a sequence-tagged (indicated with lower case letters) RRV-specific RT primer (5′-ggcagtatcgtgaattcgatgcAACACTCCCGTCGACAACAGA-3′) with SuperScript IV reverse tran-scriptase (Life Technologies). RRV genomes were quantified by RT-qPCR using a tag sequence-specific reverse primer (5′-GGCAGTATCGTGAATTCGATGC-3′ ...
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bioRxiv - Microbiology 2021Quote: Tagged diguanylate cyclases were generated by PCR amplification of each diguanylate cyclase followed by cloning into pENTR D-TOPO (ThermoFisher Scientific) and then transferred to either pRH016 (HA tag ...
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bioRxiv - Molecular Biology 2020Quote: ... Tagged amplicon pools were quantified using the Qubit™ 3.0 fluorometer and a Qubit™ dsDNA HS Assay Kit (Invitrogen, UK) and pooled with equimolar concentrations into a unique library ...
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bioRxiv - Molecular Biology 2020Quote: ... mESCs were transfected with 10ug pCAG vectors encoding 2xFlag-HA-tagged BAP1 wild-type or BAP1 C91S using Lipofectamine 2000 (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were grown on coverslips overnight and transfected with an mGFP-tagged donor construct (GFP-SHANK3 SPN WT or GFP SHANK3 SPN R12E/K22D) and mCherry-tagged acceptor construct (mCherry-KRASG12V) using Lipofectamine® 3000 (Invitrogen) or jet PRIME (Polyplus ...
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bioRxiv - Cancer Biology 2022Quote: ... MIA PaCa-2 cells were grown on coverslips overnight and transfected with GFP-tagged SPN WT or SPN R12E/K22D using Lipofectamine® 3000 (Invitrogen) for 24 h as described above ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated at RT for 1 hr with an anti-mouse secondary antibody tagged with AlexaFluor488 (Thermo Fisher Scientific, A11001) used at 2 μg/mL in blocking buffer ...
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bioRxiv - Genomics 2022Quote: ... The human urothelial bladder tumor cells were transfected with the doxycycline-inducible GFP-tagged APOBEC3G vector and the PiggyBac transposase vectors with the Lipofectamine2000 transfection agent (ThermoFisher Scientific). G418 was used for the selection of the clones with stable genomic insertion of the GFP-tagged APOBEC3G component ...
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bioRxiv - Systems Biology 2022Quote: ... Individual wells were transfected with 25ng of plasmid expressing one tagged Parkin variant using a Lipofectamine 3000 kit (Invitrogen, L3000-015) via manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK 293T cells were transiently transfected with 5 µg of FLAG tagged full-length HRI construct and 5 µg of GFP tagged DELE1 constructs (DELE1CTD or mutants) using Lipofectamine 3000 transfection reagent (L3000008, ThermoFisher Scientific). GFP empty vector was used as a negative control ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no. A-11081, Thermo Fisher Scientific, USA). The detailed immunostaining protocol is available upon request.
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... H10,14,243F GPR65 or B2AR N-terminally tagged with FLAG were cultured in DMEM high glucose (Cytiva, SH3024301) supplemented with 10% fetal bovine serum (FBS; Gibco, 26140079). Stable cell lines expressing one of the constructs were generated using Geneticin (Gibco ...
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bioRxiv - Genetics 2023Quote: ... We used Gibson Assembly to add a 3x FLAG tag to the appropriate end and insert the tagged coding sequence into a pcDNA5/FRT vector (Thermo Fisher). We note that we overexpressed the propeptide of PSMB4 (removing amino acids 2-45).
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bioRxiv - Microbiology 2023Quote: ... HA-tagged CDPK1 in the cWT and cMut lines was visualized with secondary goat antibodies (1:2000, 1 hr; Life Technologies) conjugated to Alexa Fluor 594 and 488 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice with filtered PBS and then incubated with AlexaFluor 555-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) and AlexaFluor 488-tagged donkey-anti-mouse (Molecular Probes ...
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bioRxiv - Biophysics 2022Quote: ... incubated for 5 min with 25 pM (single-label) or 5 pM (dual-label) biotin-tagged α-FLAG monoclonal antibody (ThermoFisher Scientific #MA1-91878-BTIN ...
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bioRxiv - Biophysics 2023Quote: Dextran-tagged Rhodamine-B (Rhod-Dex) with 10,000 Da and 70,000 Da molecular weights were from Invitrogen (Invitrogen 11466337 and 11590226). Mouse pre-osteoblast cells were cultured on 400 nm porous and non-porous surfaces in a 96-well plate 24 hours before the incubation with Rhod-Dex ...
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bioRxiv - Biochemistry 2023Quote: ... 24 hours later cells were transfected with 2 ug DNA (mCherry tagged PPP1R15A DNA and empty carrier DNA at a ratio of 1:19) using the lipofectamine LTX system (Life Technologies) at a ratio of 3 μl lipofectamine LTX to 1 μl Plus reagent for every nanogram of DNA in 200 μl of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: Fifty wildtype or myc-tagged Sox3 expressing animal pole cell explants were crosslinked for 15 minutes with 1% methanol-free formaldehyde (Life Technologies) and the reaction was quenched with 125 mM glycine in 0.5% PBST (Triton X-100) ...
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bioRxiv - Biochemistry 2024Quote: FL α-syn was covalently tagged at its lysine residues using NHS-Rhodamine (ex. 552 nm, em. 575 nm, Thermo Scientific). The probe was added to 100 µM α-syn in ∼ 8-fold molar excess ...
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bioRxiv - Microbiology 2024Quote: ... N-terminal FLAG-tagged CBP/p300 cDNA was amplified by PCR and was then inserted into pcDNA3.1 (Invitrogen Thermo, Carlsbad, CA).
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bioRxiv - Genetics 2021Quote: ... Cas9 protein (Invitrogen), a single-stranded 100-bp oligo donor nucleotide (ssODN ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein gel (Invitrogen). Antibodies were added according to the manufacturers recommended conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems] ...
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bioRxiv - Biochemistry 2020Quote: ... Purified PCR products were digested with XhoI and NheI and inserted into the pcDNA6/V5-His expression vector (Invitrogen) generating plasmid pMBA40 for POMGNT1 complementation ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA libraries were pooled for emulsion PCR using an Ion PI Hi-Q Chef Kit (Thermo Fisher Scientific). The enriched samples were loaded onto an Ion PI chip v3 with Ion Chef ...
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bioRxiv - Genomics 2021Quote: ... Sequencing was performed on the Ion Torrent PGM with the Ion PGM Hi-Q View Sequencing kit (Life Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the RNA-Seq templates were prepared using the Ion PGM Hi-Q View OT2 Kit (Thermo Fisher Scientific Inc.) on an Ion OneTouch 2 system ...
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bioRxiv - Microbiology 2020Quote: 293F cells were transfected by plasmids pOptiVec/V5-His WEAU gp120-trimer using 293fectin according to the manufacturer’s (Invitrogen) instructions ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... and C-terminal foldon trimerization motif followed by hexa-His tag) were used to transiently transfect Expi293F cells (Gibco). Four days after transfection ...