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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Secondary hybridization was performed using fluorescently tagged antibodies: AF647 goat anti-rabbit (A2l24S, Invitrogen, l:l000), AFS94 goat anti-rabbit (All037 ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T and 8E11 cell lines were maintained in DMEM medium containing 10% FBS and Pen/Strep (Invitrogen). RK13 cells were maintained in EMEM medium containing 10% FBS and Pen/Strep (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Pen-Strep-Glutamine (10.000 U/ml penicillin, 10.000 μg/ml streptomycin, 29.2 mg/ml L-glutamine (Invitrogen)) and 50 μM β-mercaptoethanol (AppliChem)) ...
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bioRxiv - Molecular Biology 2021Quote: ... 100x Penicillin/Streptomycin/L-Glutamine solution (10.000 U/ml Pen.; 10.000 μg/ml Strep., 29.2 mg/ml L-Glutamine) (Gibco)] ...
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bioRxiv - Molecular Biology 2022Quote: ... 20µL of the first Strep elution was loaded on Nu-PAGE 4%–12% Bis-Tris gels (Invitrogen) and analyzed via silver staining ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was collected by centrifugation and resuspended in Dulbecco’s modified Eagle’s medium: F12 medium (DMEM:F12) containing Pen/Strep antibiotics (Invitrogen) and Glutamax (Invitrogen) ...
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bioRxiv - Developmental Biology 2020Quote: ... Animals were then transferred into 250 ml of S-Medium supplemented with 1% Penn/Strep (Life Technologies), 0.1% nystatin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in DMEM/F12 with glutamax supplemented with 15% FBS and 1% pen/strep (Thermo Scientific, CA) and plated on Collagen I-coated 12-well tissue culture plates (#354500 ...
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bioRxiv - Genetics 2019Quote: ... 20 mM L-glutamine and 1X Pen/ Strep solution and transfected using the Neon™ system (Invitrogen). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... 20µL of the first elution Strep was loaded on Nu-PAGE 4%–12% Bis-Tris gels (Invitrogen) and analyzed via silver staining.
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bioRxiv - Neuroscience 2021Quote: ... coated dishes with mTeSR 1 medium (05851, Stemcells Technologies) supplemented with Pen/Strep 1% (15070-063, ThermoFisher). Before differentiation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and supplemented with Pen/Strep (1%, Bioconcept, Allschwil, CH) and 2% fetal bovine serum (FBS, Gibco, Thermofisher), at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and supplemented with Pen/Strep (1%, Bioconcept, Allschwil, CH) and 2% fetal bovine serum (FBS, Gibco, Thermofisher), at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Microbiology 2021Quote: ... and 100 U/ml of penicillin and 100 μg/ml of streptomycin (Pen/Strep; Invitrogen, Carlsbad, CA). 293T cells and Vero E6 cells (ATCC CRL-1586 ...
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bioRxiv - Developmental Biology 2021Quote: ... Animals were then transferred into 250 ml of S-Medium supplemented with 1% Penn/Strep (Life Technologies), 0.1% nystatin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... constituted by DMEM supplemented with Pen-Strep antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin; Gibco), 10% fetal bovine serum (FBS ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... (2% B27 supplement, 0.5% glucose, 1X Glutamax, 1% Pen/Strep, in Neurobasal medium –phenol red, all Gibco) in a 5% CO2 humidified incubator at 37 °C as previously described (Vidaki et al. ...
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bioRxiv - Bioengineering 2022Quote: ... sodium pyruvate (11360070) and penicillin/streptomycin (pen/strep. 10378016) solutions were purchased from Fisher Scientific (Waltham, MA). The High Capacity cDNA Reverse Transcription kit (4368814 ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 100 uL per well of secondary Anti-Strep-HRP (1:5,000 dilution) (Thermo Scientific) were plated for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: AriA and AriB-strep were co-expressed from a pRSF-Duet plasmid in BL21-AI cells (Invitrogen). Cultures grown at 37°C to 0.4-0.5 OD600 were induced with 0.1 mM IPTG and 0.1% L-arabinose ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mg ml−1 Streptomycin (1X Pen/Strep) and 1 X non-essential amino acids (Life Technologies). HeLa Flp-In TREx cells were cultured using DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Cancer Biology 2024Quote: ... cell media were supplemented with Pen Strep (100 U/mL penicillin and 100 ug/mL streptomycin, Gibco).
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bioRxiv - Biochemistry 2023Quote: ... 100 mg ml−1 Streptomycin (1X Pen/Strep) and 1 X non-essential amino acids (Life Technologies). HEK293 Flp-In TREx cells/Hela Flp-In cells were cultured using DMEM (Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Cell Biology 2022Quote: ... FUCCI-hESCs cells were seeded into 6 well plates using medium without Penicillin-Streptomycin (Pen/Strep, Gibco), in low density ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% fetal calf serum (FCS) and 0.5% penicillin-streptomycin (pen-strep, catalog number 15140, Life Technologies) at 37°C under 5% CO2 and were passaged twice a week ...
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bioRxiv - Neuroscience 2023Quote: ... 1% Pen-Strep by volume (v/v)) at 37 °C/5% CO2 in chambered slides (Thermo Fisher Scientific Nunc Lab-Tek II CC2 Chamber Slide ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 100 IU/mL of penicillin/100 µg/mL of streptomycin (Pen-Strep; Thermo Fisher Scientific), and 1.25 µg/mL of amphotericin B (AmpB ...
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bioRxiv - Neuroscience 2023Quote: ... and the entire small intestine was removed and lavaged with PBS containing penicillin-streptomycin (Pen-Strep; Invitrogen), then cut into 2-cm-long segments ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellet was collected by centrifugation and resuspended in Dulbecco’s modified Eagle’s medium: F12 medium (DMEM:F12) containing Pen/Strep antibiotics (Invitrogen) and Glutamax (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... and were kept in IntestiCult OGM supplemented with 100 μg/ml Pen/Strep (Catalog no. 15140122, Gibco) for maintenance ...
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bioRxiv - Neuroscience 2023Quote: ... and the entire small intestine was removed and lavaged with PBS containing penicillin-streptomycin (Pen-Strep; Invitrogen). The small intestine was then cut into 1-cm-long segments ...
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bioRxiv - Microbiology 2024Quote: ... Pen-Strep (10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin) was acquired from Gibco, USA ...
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bioRxiv - Neuroscience 2024Quote: ... larvae were unmounted from agarose and placed into normal Ringer’s solution+1% Pen Strep (Gibco, 15140-122) until imaging at 10-11 dpf ...
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bioRxiv - Developmental Biology 2023Quote: ... These were recombined into a pDEST II backbone using LR Clonase II (Invitrogen, P/N56485).
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Raw MS Data were searched against the human SwissProt protein database (Version November 2020) with Proteome Discoverer 2.4 (ThermoFisher Scientific, Bremen, Germany) using the following parameters ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: ... All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) and purified as previously described (4 ...
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bioRxiv - Neuroscience 2022Quote: ... similarly as described previously.[60] MS data were analyzed using an in-house maintained human protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific). The mass tolerances of a fragment ion and a parent ion were set as 0.5 Da and 10 ppm ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2023Quote: Plasmids coding for the soluble S ectodomain proteins as well as the RBD of human coronaviruses were transfected into Freestyle293F cells (Thermo Fisher R79007). Per 1 L of Freestyle 293F cells ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
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bioRxiv - Genetics 2021Quote: ... We first generated an atlas-GFP DNA construct by cloning the atlas protein-coding sequence into pENTR and using LR Clonase II (Thermo Fisher Scientific, Waltham, MA) to recombine the sequence with pTWG (DGRC #1076 ...
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bioRxiv - Biochemistry 2022Quote: ... glycerol] and lysed by sonication (Branson Sonifier 250, Emerson, USA) The lysates were used for protein purification by Ni(II)-NTA affinity chromatography (Invitrogen, Thermo Fisher Scientific, USA) as previously described.[58] Desalting was carried out using a PD-10 column (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...