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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were quantified using Coomassie Protein Assay Reagent (ThermoFisher). Protein samples (30 μg ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using Qubit Protein Assay (Invitrogen). Lysates were diluted to the desired concentration with 20 mM HEPES pH 7.2 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were washed three times again in PBS with 0.2% Triton X-100 and transferred into secondary antibody solution (anti-chicken Alexa Fluor 488, 1:200; anti-mouse Alexa Fluor 647, 1:200; anti-rabbit Alexa Fluor 647, 1:200; Molecular Probes, Eugene, OR, USA) and incubated for 48 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 1:200) at 4 °C 48 hours and in secondary antibodies simultaneously (Goat Anti-mouse 1:200, Invitrogen, Goat Anti-Rabbit 1:200, Jackson) at RT for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-alpha-Tubulin AA4.3-c (1:5000; DSHB)) were detected using HRP-conjugated secondary antibodies (1:10000; Thermo Fisher) and ECL Prime kit (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-mouse Alexa Fluor 546 immunostaining (Thermo Fisher #A-11003 at 1:500 for 1 hr at 37 °C), and Hoechst 33342 staining (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used were 1:400 anti-rabbit Alexa 488 and 1:250 anti-mouse Alexa 594 from Invitrogen. The cells were washed thrice in 1X PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-RBPMS (1:200; 91. Secondary antibodies used were: Alexa Fluor 488 donkey anti-mouse (1:500; ThermoFisher, A32766); Alexa Fluor 488 donkey anti-rabbit (1:500 ...
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bioRxiv - Genetics 2021Quote: ... Alexa Fluor 488 Goat Anti-mouse IgG (1:300) and Alexa Fluor 568 Goat Anti-rabbit IgG (1:300) from Invitrogen. Digital images were obtained using Leica TCS SP8 LIGHTNING confocal microscope equipped with motorized stage and LAS X software ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated overnight at 4°C with the following primary antibodies at a concentration of 1:500: mouse anti-V5 (1:500, Invitrogen), rabbit anti-γ-H2AX (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: mouse anti-brp (1:50, Developmental Studies Hybridoma Bank #nc82) and rabbit anti-GFP (1:1000, Invitrogen), with secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... and then immunostained with mouse anti-NaV1.1 (1:200; UC Davis/NIH NeuroMab Facility; K74/71) and Alexa 488-conjugated anti-mouse IgG1 secondary antibodies (ThermoFisher). Images were acquired with a Zeiss 700 confocal ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... (IF 1:200) and Alexa Fluor 647-conjugated polyclonal chicken anti-mouse antibody (A21463) (IF 1:200) were purchased from Invitrogen. Alexa Fluor 488 phalloidin was purchased from Invitrogen (whole-mount stain 1:200) ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the recommended dilution of HRP-conjugated secondary antibody (goat anti-rabbit IgG HRP 1:10000, Invitrogen; goat anti-mouse IgG HRP 1:10000, Invitrogen) at room temperature for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit Alexa Fluor 488 IgG H+L (1:400, A11008) and goat anti-mouse Alexa Fluor 568 IgG H+L (1:250, A11004) (Invitrogen, ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with anti-mouse or anti-goat Alexa Fluor 488 and Alexa Fluor 594 antibodies (1:400; 1 hr at RT; Invitrogen) and counter-stained with DAPI nuclear dye (0.5 µg/ml in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Alexafluor 488 F(ab’)2 fragment of goat anti-rabbit IgG (H+L) (1:100) and Alexafluor 488 goat anti-mouse IgG (H+L) (1:100) were all purchased from ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... using the appropriate antibody (1:10,000; rabbit polyclonal PA1-16889, Thermo Fisher or 1:10,000; mouse monoclonal MA1-140, Thermo Fisher) alongside with the targets of interest ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight with primary antibody (Thermo Fisher rabbit anti-GFP, 1:1,000; Thermo Fisher mouse anti-HA, 1:5,000). HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... or mouse antibody to Myc (1:2000, Sta. Cruz) and Clean-Blot IP detection reagent (HRP) (1:5000, Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... and washed in PBS two additional times before adding secondary anti-goat Cy5 (1:2500, 072-02-13-06, KPL) or anti-mouse Alexa Fluor 647 (1:2500, A32728, Invitrogen) and incubated at 4°C in the dark for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3 hour incubation with Alexa Fluor 647-conjugated secondary antibody (donkey anti guinea pig, 1:1000) or Cy3-conjugated secondary antibody (goat anti mouse,1:1000, Invitrogen) respectively.
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bioRxiv - Bioengineering 2021Quote: ... Samples were rinsed once with PBS++ and incubated with 1:100 dilution of mouse anti-ZO-1 antibody (Life Technologies) and 3:200 dilution of AlexaFluor 488 or 633 phalloidin for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were then washed and incubated for 1 min at RT with 1:5000 anti-mouse secondary antibody conjugated via Streptavidin with Qdot655 (Invitrogen) in Krebs solution supplemented with 10% Horse Serum and 1:1000 DMSO or forskolin ...
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bioRxiv - Cell Biology 2022Quote: ... followed by washing with Tween-20/PBS and 1 h incubation with HRP-conjugated goat anti-mouse IgG at 1:40 000 (Cat# A16066, Invitrogen) in 100 µl per well of Tween-20/PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then equilibrated in PBS and incubated 1 hour with anti-mouse Alexa flour 488 (1:2000, Thermo Fisher) and anti-rabbit Alexa flour 647 (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30 ...
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bioRxiv - Biochemistry 2020Quote: ... and then for 1 h with a 1/10 000 dilution of anti-mouse– horseradish peroxidase conjugate (Thermo Fisher Scientific). His-tagged proteins bound to the membrane by virtue of its interaction with lipid ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were washed in PBS before incubation with mouse anti-BrdU (B44, Becton Dickinson 347580, 1:200) for 1 h and subsequently with antimouse IgG AlexaFluor 488 (ThermoFisher) for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Blots were washed and then incubated for 1 hr with 1:10000 goat anti-mouse IgG-HRP (62-6520; Invitrogen). Blots were imaged using chemiluminescent buffer (Perkin Elmer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1 h with the secondary antibody which was donkey anti–mouse coupled to Alexa Fluor 488 (Invitrogen, 1:500). Alternatively ...
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bioRxiv - Immunology 2020Quote: ... or anti-mouse IgM AF488 conjugated secondary antibodies were added at 1:500 dilution for 1 hour at RT (Invitrogen). Confocal imaging was performed with a Zeiss LSM 980 using a 63x Plan-Achromatic 1.4 NA oil immersion objective ...
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bioRxiv - Immunology 2021Quote: ... and incubated with goat anti-mouse IgG-HRP (1:5000, Cwbiotech) or goat anti-monkey IgG-HRP (1:10000, Invitrogen) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibody in PBS supplemented with 3% BSA (Thermofisher, Mouse 800, 1:20000 and Thermofisher, Rabbit 680, 1:20000) was incubated in the dark for 1 hour on a rolling platform at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and mouse anti-Dlg (1:500, Developmental Studies Hybridoma Bank) and secondary antibodies goat anti-rabbit Alexa 488 (1:500, Invitrogen) and goat anti-mouse Alexa 568 (1:500 ...
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with peroxidase-conjugated goat anti-rabbit (1:5000) or goat anti-mouse (1:50000) (both from Invitrogen). Pierce™ ECL Western Blotting Substrate was used to detect protein-antibody complexes prior to visualization on CL-X Posure™ films ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies used were mouse anti-rabbit (1:10’000, Jackson, 211-032-171) and rabbit anti-sheep (1:10’000, Invitrogen, 81-8620).
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bioRxiv - Microbiology 2022Quote: ... for 1 hour at room temperature followed by additional 1 hour incubation with 1:200 dilution of fluorescence-tagged goat anti-mouse IgG Alexa Fluor 488 (Thermofisher) (Supplemental Table 5) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3) (60) followed by secondary Alexa Fluor 488 goat α-mouse IgG antibody (Invitrogen / Thermo Fisher Scientific – 1:1,000).
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-GAPDH, 1:5000 (CAB932Hu22, USCN) and horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat anti-mouse, 1:5000, G-21040, Invitrogen; Goat anti-rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... an IFA with anti-CSP antibody (1:500) followed by anti-mouse Alexafluor 488 (1:1,000; Invitrogen, Carlsbad, CA, USA) labels uninvaded parasites ...
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bioRxiv - Biochemistry 2024Quote: ... At the next day blots were washed 3x with TBST for 10 min and incubated with HRP conjugated secondary antibodies (goat anti mouse HRP, 1:1000, 34130 and goat anti rabbit, 1:1000, 34160, Thermofisher) for 1h ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 % BSA) and stained with secondary antibodies goat anti-mouse IgG (H+L) Alexa Fluor®488 (Invitrogen, 1:500), donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... and stained with conjugated primary and secondary antibodies (a-mouse AF594, 1:200, ThermoFisher and phalloidin AF488, 1:500, ThermoFisher), and imaged using a confocal microscope ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 45 minutes with 1:500 AlexaFluor 488-conjugated anti-mouse (Invitrogen, 1:500 in 4% BSA PBS) plus DAPI (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-mouse HRP-conjugated (G-21040, 1:10000) and anti-rabbit HRP-conjugated (65-6120, 1:10000) secondary antibodies from Invitrogen.
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bioRxiv - Microbiology 2022Quote: ... The plates were washed three times with PBS with 0.05% Tween 20 and then incubated for 1 h at RT with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (1:5,000, Cat# 62-6520, ThermoFisher Scientific), IgG1 (1:5,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... sections were incubated for 1 hour with a secondary antibody cocktail containing 1:250 anti-mouse IgG1 AF594 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies were used at 1 μg mL-1 for goat anti-rabbit and sheep anti-mouse linked horseradish peroxidase (Invitrogen). Blots were developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce).