Labshake search
Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... The cell pellet was resuspended in 2mL medium with sheep-anti-rat IgG Dynabeads™ (Cat#: 110-35, Invitrogen) coupled with a CD31 antibody (clone Mec13.3 ...
-
bioRxiv - Microbiology 2024Quote: ... The remainder of the lysate was incubated with 0.1 mL of Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen) previously conjugated with 10 ug of anti-HA antibody (Millipore ...
-
bioRxiv - Microbiology 2021Quote: ... and 25 ng linear pPolI-SARS-CoV2-NLuc-N plasmid using 2μl of Lipofectamine 2000 (Invitrogen) to a DNA:lipofectamine ratio of 1:3 and 100μl Opti-MEM (Gibco) ...
-
bioRxiv - Genomics 2021Quote: We measured the dsDNA amount of combined S1 and S2 fraction using Qubit® 4.0 fluorometer (Thermo Fisher Scientific) by Equalbit® 1×dsDNA HS Assay Kit (Vazyme ...
-
bioRxiv - Molecular Biology 2021Quote: ... forward primers containing the attB1 site and reverse primers containing the attB2 site (Table S1) were purchased from Invitrogen. These primers were used to amplify the F ...
-
bioRxiv - Biochemistry 2021Quote: The extracellular part of ACE2 (Q18 – S740) and RBD protein variants (Table S1) were produced in Expi293F cells (ThermoFisher). Pure DNA was transfected using ExpiFectamine 293 Transfection Kit (ThermoFisher ...
-
bioRxiv - Microbiology 2020Quote: ... RT-qPCR was carried out using primers specific (Table S1) with SYBR Green Real-Time PCR Master Mixes (Invitrogen) and the StepOnePlus Real System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA of the genes of interest (Table S1) was pre-amplified with TaqMan® PreAmp master mix (Invitrogen™) and equimolar mixture of forward and reverse strand primers designed for the genes of interest (Table S3) ...
-
bioRxiv - Neuroscience 2021Quote: ... a fragment (Table S1) encoding the 20AA axon targeting motif from Broussard et al.,20 was synthesized (Invitrogen, GeneArt) and ligated into jREX-GECO1 and GCaMP8s and m plasmids ...
-
bioRxiv - Neuroscience 2022Quote: The total amount of soluble tau in S1 fractions was determined by using an ELISA kit (human Tau, Invitrogen), according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Fractions were collected and analyzed by western blotting with polyclonal antiserum against the S1 domain (Thermo Fisher, Cat# PA581798). Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO ...
-
bioRxiv - Cancer Biology 2021Quote: ... Following overnight incubation with the immunoprecipitating antibody (Supplementary Table S1) or the IgG isotype control (Rabbit Isotype Control (Thermofisher, Cat ...
-
bioRxiv - Cancer Biology 2021Quote: ... Following overnight incubation with the immunoprecipitating antibody (Supplementary Table S1) or the Rabbit Isotype Control (Thermofisher, Cat. No 10500C) and 4h of incubation with Pierce™ Protein A/G Magnetic Beads (Thermofisher ...
-
bioRxiv - Microbiology 2021Quote: ... Fractions were collected and analyzed by western blotting with polyclonal antiserum against the S1 domain (Thermo Fisher, Cat# PA581798). Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO ...
-
bioRxiv - Microbiology 2020Quote: ... treatment was carried out after inactivation of protease with 0.56 u/µl nuclease in S1 nuclease buffer (ThermoFisher Scientific) for 60 min at 37°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... The cDNA formed was immediately pre-amplified using Taqman gene expression arrays (supplementary table S1, Fisher Scientific, Loughborough, UK) and Taqman PreAmp Mastermix (Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... Following overnight incubation with the immunoprecipitating antibody (Supplementary Table S1) or the Mouse Isotype Control (Thermofisher, Cat. No 10400C) at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... The 30S-S1 subunits were pelleted via ultracentrifugation with a Sorvall S55-S swinging-bucket rotor ultracentrifuge (Thermo Scientific) at 55 000 rpm ...
-
bioRxiv - Microbiology 2022Quote: ... PCR was performed using flanking primers mentioned in Table S1 to screen candidate colonies using Dreamtaq Master Mix (ThermoFisher). PCR products were analyzed by agarose gel electrophoresis and Sanger sequencing (Genewiz ...
-
bioRxiv - Biophysics 2023Quote: ... Recombinant bacmids were generated from the plasmids in Table S1 using MAX Efficiency DH10Bac competent cells (Gibco; Cat. 10361012) and transfected into Sf9 insect cells (Gibco ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 μg of total RNA or 1.5 μg of nuclear RNA were reverse transcribed with 0.5 μM TeloR and 0.05 μM U6 oligonucleotides (#18 and #217, respectively; Supplemental Table S1) using the SUPERSCRIPT IV reverse transcriptase (Invitrogen). Quantitative PCRs were performed with specific oligonucleotide pairs as indicated in Supplemental Table S1 using the iTaq Universal SYBR Green Supermix (BioRad ...
-
bioRxiv - Molecular Biology 2022Quote: ... using gene-specific PCR primers (Supplemental Table S1) mixed with Power SYBR Green Master Mix (Thermo Fisher Scientific, 4367659) and diluted cDNA (10-fold dilution ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA served as template in qPCR with gene-specific primers (S1 Table) and SYBR Green Master Mix (Applied Biosystems) using a StepOnePlus system according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Pelleted cells were incubated with an antibody cocktail described in Table S1 with LIVE/DEAD Fixable Aqua (Invitrogen, #L34957) for 20 minutes at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... third ventricle and bilateral SCN tissues (Fig. S1) was selected and transferred on to pre-chilled glass slides (ThermoFisher Scientific SuperFrost Plus Adhesion slides ...
-
bioRxiv - Immunology 2024Quote: ... BA.2.86 and H-CoV S proteins were transiently transfected in ExpiCHO-S cells (Thermo Fisher) using ExpiFectamine™ CHO Reagent ...
-
bioRxiv - Genetics 2021Quote: ... we added 1 µl of 1:100 dilution of ERCC spike-in mix 1 (Thermo Fisher, 4456740, LOT00418382) to 0.5–1µg total RNA to enable subsequent molecular quantification of transcripts ...
-
bioRxiv - Biochemistry 2021Quote: ... for spike protein or RBD detection and mouse anti-beta tubulin antibody (1:5000; Thermo Fisher; MA5-16308) as a loading control ...
-
bioRxiv - Cell Biology 2022Quote: ... The cell number was used to adjust the amount of ERCC RNA Spike-In Mix (ThermoFisher Scientific, 4456740). Generation of RNA-seq libraries (TruSeq Stranded Total RNA Sample Library Prep ...
-
bioRxiv - Microbiology 2021Quote: ... Virion lysates were analyzed by SDS-PAGE and western blot using Spike Protein S2 Monoclonal Antibody (1A9) (Invitrogen) (1:1000 dilution ...
-
bioRxiv - Microbiology 2020Quote: ... The spike protein gene cDNA was amplified with a SuperScript IV One-Step RT-PCR kit (ThermoFisher Scientific) using primers flanking the S gene ...
-
bioRxiv - Systems Biology 2020Quote: ... ERCC spike-in RNA (2µl diluted at 1:100) was added to each RNA extraction (Ambion, cat:4456740). A total of 9 cDNA libraries were prepared with an NEBNext Ultra II RNA Library Prep kit (NEB ...
-
bioRxiv - Immunology 2020Quote: To this aim the stabilized Spike protein was coupled to Streptavidin-PE (eBioscience # 12-4317-87, Thermo Fisher) for 30 min at 4°C and then incubated with VERO E6 cells ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl of 1:100 dilution of ERCC spike-in mix 1 (Thermo Fisher, 4456740, LOT00418382; Table S5) was added to 0.5–1 μg total RNA to enable absolute quantification of mRNA ...
-
bioRxiv - Immunology 2021Quote: ... B.1.351 or P.1 Spike protein were produced using HEK 293T cells transfected with Lipofectamine 3000 (ThermoFisher) using IgE-SARS-CoV-2 S plasmid variants (Genscript ...
-
bioRxiv - Immunology 2020Quote: ... To this aim the stabilized Spike protein was coupled to Streptavidin-PE (eBioscience # 12-4317-87, Thermo Fisher) for 1h at room temperature and then incubated with VERO E6 cells ...
-
bioRxiv - Immunology 2020Quote: ... or onto an SRT SEC-1000 4.6 x 300 mm (spike proteins) column equilibrated in 1X PBS [pH 7.4] or 1X Dulbecco’s phosphate-buffered saline (DPBS) (Gibco). Columns were flowed at a rate of 0.15 mL/min (S200 ...
-
bioRxiv - Developmental Biology 2020Quote: ... 500 ng RNA from cells that was spiked-in with ERCC Exfold Spike-in mixes (Thermo Fisher, 4456739) was used for the generation of RNA-seq libraries ...
-
bioRxiv - Immunology 2022Quote: ... Pseudotyped viruses were generated by transfection 293T cells (ATCC, CRL-3216) with pcDNA3.1-Spike with Lipofectamine 3000 (Invitrogen). The cells were subsequently infected with G*ΔG-VSV (Kerafast ...
-
bioRxiv - Immunology 2022Quote: ... Genes encoding the Omicron (BA.5) Spike was synthesized and cloned by GeneArt (Thermo Fisher Scientific, Waltham, MA) in the pcDNA3.4 expression vector ...
-
bioRxiv - Microbiology 2022Quote: ... and then purifying the spike trimer from the supernatants five days post-transfection using Ni-NTA resin (Invitrogen) according to the manufacturer’s instructions27 ...
-
bioRxiv - Cell Biology 2020Quote: ... Human PAR4 containing an N-terminal V5-epitope were stably expressed using the HEK293 Flp-In T-Rex cells according to the manufacturer’s protocol (Invitrogen). The concentration of tetracycline was titrated (700 ng/mL ...
-
bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were transfected with either NSUN6 wild-type and the miCLIP-mutant construct using Lipofectamine 2000 (Life Technologies) and harvested 24 hours later.
-
bioRxiv - Molecular Biology 2020Quote: ... GFP-N was immunoprecipitated from two independent HEK293 cell pellets using 5μg of anti-GFP antibody (Life Technologies G10362). A total of 2% input material was obtained for size-matched control libraries (SMI ...
-
bioRxiv - Molecular Biology 2020Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (Invitrogen catalogue number R780-07). Cell cultures were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
-
bioRxiv - Genetics 2019Quote: HEK293 cells (CRL-1573, ATCC, VA, USA) were cultured in high-glucose DMEM (Cat. No. 11965092, Life Technologies, USA) supplemented with 10% FBS at 37 and 5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... Human parental HEK293 cells (ATCC, CRL-1573) were cultured in DMEM supplemented with 10% FBS and antibiotics/antimycotics (Gibco).
-
bioRxiv - Biophysics 2021Quote: ... The HEK293 cells were transfected with 100 ng/well of pGL3-Fluc plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol at around 75-90% confluency in a 96 well plate ...